Fluorescence lifetime techniques in medical applications.

abstract：:We seek to construct physical and mathematical models of life. Such models allow us to test our understanding of how living systems function and how they respond to human imposed stimuli. One system is a genomically and chemically complete model of a minimal cell. This cell is a hypothetical bacterium with the fewest ...

journal_title：Annals of biomedical engineering

authors： Shuler ML

更新日期：2012-07-01 00:00:00

abstract：:Induced pluripotent stem cell derived cardiomyocytes (iPSC-CM) provide a powerful platform for disease modeling and drug development in vitro. Traditionally, electrophysiological methods or fluorescent dyes (e.g. calcium) have been used in their functional characterization. Recently, video microscopy has enabled non-i...

journal_title：Annals of biomedical engineering

authors： Ahola A,Pölönen RP,Aalto-Setälä K,Hyttinen J

更新日期：2018-01-01 00:00:00

abstract：:We and others have shown that changing surface characteristics of the spacer implanted during the first Masquelet stage alters some aspects of membrane development. Previously we demonstrated that titanium (TI) spacers create membranes that are better barriers to movement of solutes > 70 kDa in size than polymethyl me...

journal_title：Annals of biomedical engineering

authors： Toth Z,Roi M,Evans E,Watson JT,Nicolaou D,McBride-Gagyi S

更新日期：2019-01-01 00:00:00

abstract：:Interstitial fluid flow, critical for macromolecular transport, was recently shown to drive fibroblast differentiation and perpendicular cell and matrix alignment in 3D collagen cultures. Here we explore the mechanisms underlying this flow-induced cell and collagen alignment. Cell and matrix alignment was assessed fro...

journal_title：Annals of biomedical engineering

authors： Ng CP,Swartz MA

更新日期：2006-03-01 00:00:00

abstract：:Tissue engineering strategies have utilized a wide spectrum of synthetic and naturally-derived scaffold materials. Synthetic scaffolds are better defined and offer the ability to precisely and reproducibly control their properties, while naturally-derived scaffolds typically have inherent biological and structural pro...

journal_title：Annals of biomedical engineering

authors： Allen AB,Priddy LB,Li MT,Guldberg RE

更新日期：2015-03-01 00:00:00

abstract：:Opportunities to provide clinical immersion experiences to bioengineering undergraduate students have expanded over the last several years. These programs allow students to observe the clinical environment in order to better understand workflow processes, the context in which medical equipment is used, and identify un...

journal_title：Annals of biomedical engineering

authors： Kotche M,Felder AE,Wilkens K,Stirling S

更新日期：2020-09-01 00:00:00

abstract：:Wall Shear Stress (WSS) topological skeleton, composed by fixed points and the manifolds linking them, reflects the presence of blood flow features associated to adverse vascular response. However, the influence of WSS topological skeleton on vascular pathophysiology is still underexplored. This study aimed to identif...

journal_title：Annals of biomedical engineering

authors： Morbiducci U,Mazzi V,Domanin M,De Nisco G,Vergara C,Steinman DA,Gallo D

更新日期：2020-12-01 00:00:00

abstract：:High mechanical stress in atherosclerotic plaques at vulnerable sites, called critical stress, contributes to plaque rupture. The site of minimum fibrous cap (FC) thickness (FC(MIN)) and plaque shoulder are well-documented vulnerable sites. The inherent weakness of the FC material at the thinnest point increases the s...

journal_title：Annals of biomedical engineering

authors： Teng Z,Sadat U,Li Z,Huang X,Zhu C,Young VE,Graves MJ,Gillard JH

更新日期：2010-10-01 00:00:00

abstract：:Rod photoreceptors express a unique type of noninactivating potassium channels, Kx channels, which play an important role in setting the dark potential and participate in shaping the light response. Biophysical studies of Kx channels are limited. For example, the effects of a conventional blocker of potassium channels...

journal_title：Annals of biomedical engineering

authors： Liu XD,Kourennyi DE

更新日期：2004-10-01 00:00:00

abstract：:Percutaneous stent implantation has revolutionized the clinical treatment of occluded arteries. Nevertheless, there is still a large unmet need to prevent re-occlusion after implantation. Consequently, a niche exists for a cost-effective pre-clinical method of evaluating novel interventional devices in human models. T...

journal_title：Annals of biomedical engineering

authors： Punchard MA,O'Cearbhaill ED,Mackle JN,McHugh PE,Smith TJ,Stenson-Cox C,Barron V

更新日期：2009-07-01 00:00:00

abstract：:Glaucoma is a common optic neuropathy characterized by retinal ganglion cell death. Elevated intraocular pressure (IOP), a key risk factor for glaucoma, leads to significant biomechanical deformation of optic nerve head (ONH) cells and tissues. ONH astrocytes respond to this deformation by transforming to a reactive, ...

journal_title：Annals of biomedical engineering

authors： Mulvihill JJE,Raykin J,Snider EJ,Schildmeyer LA,Zaman I,Platt MO,Kelly DJ,Ethier CR

更新日期：2018-02-01 00:00:00

abstract：:Using a three-dimensional propagation model of the human ventricular myocardium, we studied the role of fibrous structure in generating epicardial potential maps. This model represents the myocardium as an anisotropic bidomain with an equal anisotropy ratio, and it incorporates a realistic representation of anatomical...

journal_title：Annals of biomedical engineering

authors： Hren R,Nenonen J,Horácek BM

更新日期：1998-11-01 00:00:00

abstract：:Cerebral blood flow (CBF) increases as arterial oxygen content falls with hypoxic (low PO2), anemic (low hemoglobin) and carbon monoxide (CO) (high carboxyhemoglobin) hypoxia. Despite a higher arterial PO2, CO hypoxia provokes a greater increase in CBF than hypoxic hypoxia. We analyzed published data using a compartme...

journal_title：Annals of biomedical engineering

authors： Sharan M,Popel AS,Hudak ML,Koehler RC,Traystman RJ,Jones MD Jr

更新日期：1998-01-01 00:00:00

abstract：:The blood-brain barrier (BBB) is a major obstacle for drug delivery to the brain. To seek for in vitro BBB models that are more accessible than animals for investigating drug transport across the BBB, we compared four in vitro cultured cell models: endothelial monoculture (bEnd3 cell line), coculture of bEnd3 and prim...

journal_title：Annals of biomedical engineering

authors： Li G,Simon MJ,Cancel LM,Shi ZD,Ji X,Tarbell JM,Morrison B 3rd,Fu BM

更新日期：2010-08-01 00:00:00

abstract：:Viscous shear stress and Reynolds stress are often used to predict hemolysis and thrombosis due to flow-induced stress on blood elements in cardiovascular devices. These macroscopic stresses are distinct from the true stress on an individual cell, which is determined by the local microscale flow field. In this paper t...

journal_title：Annals of biomedical engineering

authors： Quinlan NJ,Dooley PN

更新日期：2007-08-01 00:00:00

abstract：:The second equation in the section "Definition of Objective Functions and Constraints" was corrected to fix a mismatch between the PDF and HTML versions of the article. ...

journal_title：Annals of biomedical engineering

authors： Lv C,Wang S,Shi C

更新日期：2020-01-01 00:00:00

abstract：:Finite element analysis is a powerful tool for investigating the biomechanics of atherosclerosis and has thereby provided an improved understanding of acute myocardial infarction. Structural analysis of arterial walls is traditionally performed using geometry contours derived from histology. In this paper we demonstra...

journal_title：Annals of biomedical engineering

authors： Chau AH,Chan RC,Shishkov M,MacNeill B,Iftimia N,Tearney GJ,Kamm RD,Bouma BE,Kaazempur-Mofrad MR

更新日期：2004-11-01 00:00:00

abstract：:Thorax injuries mainly due to rib fractures have been associated with high rates of morbidity and mortality in motor vehicle crashes. Thoracic biomechanics has been studied extensively, but there are no robust biomechanical response targets for ribs that consider age, sex, body size, and vulnerability factors. The obj...

journal_title：Annals of biomedical engineering

authors： Kang YS,Kwon HJ,Stammen J,Moorhouse K,Agnew AM

更新日期：2021-02-01 00:00:00

abstract：:Uncomplicated acute type B aortic dissections are usually treated medically, but they can become acutely complicated by rapid expansion, rupture and malperfusion syndromes and in the longer term by chronic dilatation and aortic aneurysm formation. The objective of this study is to use computational fluid dynamics reco...

journal_title：Annals of biomedical engineering

authors： Cheng Z,Wood NB,Gibbs RG,Xu XY

更新日期：2015-01-01 00:00:00

abstract：:We examine the influence of the added mass effect (fluid inertia) on mitral valve leaflet stress during isovolumetric phases. To study this effect, oscillating flow is applied to a flexible membrane at various frequencies to control inertia. Resulting membrane strain is calculated through a three-dimensional reconstru...

journal_title：Annals of biomedical engineering

authors： Bark DL Jr,Dasi LP

更新日期：2016-05-01 00:00:00

abstract：:Local hemodynamics are an important factor in atherosclerosis, from the development of early lesions, to the assessment of stroke risk, to determining the ultimate fate of a mature plaque. Until recently, our understanding of arterial fluid dynamics and their relationship to atherosclerosis was limited by the use of i...

journal_title：Annals of biomedical engineering

authors： Steinman DA

更新日期：2002-04-01 00:00:00

abstract：:Point cathodal stimulation of cardiac tissue was shown previously to produce both a dog-bone shaped virtual cathode transverse to the muscle fibers and two longitudinal virtual anodes. We hypothesize that virtual anodes can cause a region of delayed activation, separating two regions of early activation caused by the ...

journal_title：Annals of biomedical engineering

authors： Wu J,Roden DM,Wikswo JP Jr

更新日期：2000-11-01 00:00:00

abstract：:Phospholipids have been identified by thin-layer chromatography in appreciable quantities in pericardial fluid taken from 12 dogs and found to include sphingomyelin and phosphatidylcholines, -ethanolamines, -inositols and -serines--the cholines predominating. The extracts, the synthetic surfactants and a mixture of sy...

journal_title：Annals of biomedical engineering

authors： Hills BA,Butler BD

更新日期：1985-01-01 00:00:00

abstract：:The epidermal growth factor (EGF) is a growth factor with effects on many cell types and tissue. Morphometric and passive biomechanical properties were studied in isolated segments of the esophagus in 22 EGF-treated rats and 12 control rats. The rats were divided into groups with EGF treatment for 2, 4, 7, and 14 days...

journal_title：Annals of biomedical engineering

authors： Zhao J,Yang J,Vinter-Jensen L,Zhuang F,Gregersen H

更新日期：2003-06-01 00:00:00

abstract：:Intramuscular pressure (IMP) shows promise for estimating individual muscle tension in vivo. However, previous pressure measurements show high variability during isometric contraction and poor correlation with tension during dynamic contraction. We hypothesized that enhanced sensor anchoring/orientation would improve ...

journal_title：Annals of biomedical engineering

authors： O'Connor SM,Kaufman KR,Ward SR,Lieber RL

更新日期：2021-02-01 00:00:00

abstract：:The study was to explore the effects of BMP-2 gene modified canine bone marrow stromal cells (bMSCs) mediated by a nonviral PEI derivative (GenEscort™ II) in promoting bone formation in vitro and in vivo. Canine bMSCs were cultured and transfected with plasmids containing bone morphogenetic protein-2 gene (pBMP-2) or ...

journal_title：Annals of biomedical engineering

authors： Lü K,Zeng D,Zhang Y,Xia L,Xu L,Kaplan DL,Jiang X,Zhang F

更新日期：2011-06-01 00:00:00

abstract：:In physiological conditions, joint function involves continuously moving contact areas over the tissue surface. Such moving contacts play an important role for the durability of the tissue. It is known that in pathological joints these motion paths and contact mechanics change. Nevertheless, limited information exists...

journal_title：Annals of biomedical engineering

authors： Schätti OR,Gallo LM,Torzilli PA

更新日期：2016-08-01 00:00:00

abstract：:Acute cerebral ischemic events and thrombosis are associated with the rupture/erosion of carotid atherosclerotic plaques. The aim of the present study was to determine the impact of calcification deposition on the wall shear stress (WSS) and stresses within the plaques using 3D fluid-structure interaction (FSI) models...

journal_title：Annals of biomedical engineering

authors： Benitez J,Fontanarosa D,Wang J,Paritala PK,McGahan T,Lloyd T,Li Z

更新日期：2020-10-20 00:00:00

abstract：:This paper outlines a comprehensive parametric approach for quantifying mechanical properties of spatially heterogeneous thin biological specimens such as human breast tissue using contact-mode Atomic Force Microscopy. Using inverse finite element (FE) analysis of spherical nanoindentation, the force response from hyp...

journal_title：Annals of biomedical engineering

authors： Roy R,Desai JP

更新日期：2014-09-01 00:00:00

abstract：:A lumped parameter compartmental model for the nonsteady flow of the cerebrovascular fluid is constructed. The model assumes constant resistances that relate fluid flux to pressure gradients, and compliances between compartments that relate fluid accumulation to rate of pressure changes. Resistances are evaluated by u...

journal_title：Annals of biomedical engineering

authors： Sorek S,Bear J,Karni Z

更新日期：1989-01-01 00:00:00