Abstract:
:Multiple lipid and protein components of the plasma membrane of a living cell are organized, both compositionally and functionally, at different spatial and temporal scales. For instance, Rab protein domains in membranes the clathrin-coated pit, or the immunological synapse are exquisite examples of functional compartmentalization in cell membranes. These assemblies consist in part of nanoscale complexes of lipids and proteins and are necessary to facilitate some specific sorting and signaling functions. It is evident that cellular functions require a regulated spatiotemporal organization of components at the nanoscale, often comprising of countable number of molecular species. Here, we describe multiple homo-FRET-based imaging methods that provide information about nanoscale interactions between fluorescently tagged molecules in live cells, at optically resolved spatial resolution.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Ghosh S,Saha S,Goswami D,Bilgrami S,Mayor Sdoi
10.1016/B978-0-12-388448-0.00024-3subject
Has Abstractpub_date
2012-01-01 00:00:00pages
291-327eissn
0076-6879issn
1557-7988pii
B978-0-12-388448-0.00024-3journal_volume
505pub_type
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