Abstract:
:Brucellosis is a worldwide zoonotic infectious disease that has significant economic effects on animal production and human health. The host macrophage -Brucella interaction is critical to the establishment of infections. Thus, the kinetic transcriptional profile of gene expression in macrophages infected with the Brucella melitensis strain 16M was investigated in the current study using a technology based on deep sequencing. The total RNA was extracted from macrophages 0, 4, and 24 h post-infection. Data analysis showed that in the gene ontology term, the expression of genes in the endoplasmic reticulum, lysosomes, as well as those involved in programmed cell death and apoptosis significantly changed during the first 24 h post-infection. Pathway enrichment analysis indicated that the genes in the apoptosis pathway, NOD-like receptor signaling pathway, Fc gamma R-mediated phagocytosis, lysosome pathway, p53 signaling pathway, and protein processing in the endoplasmic reticulum significantly changed during the first 24 h post-infection. The B-cell receptor and toll-like receptor signaling pathways were also significantly changed 24 h post-infection compared with those 4 h post-infection. The results of the current study can contribute to an improved understanding of the manner by which host cell responses may be manipulated to prevent Brucella infection.
journal_name
Microb Pathogjournal_title
Microbial pathogenesisauthors
Liu Q,Han W,Sun C,Zhou L,Ma L,Lei L,Yan S,Liu S,Du C,Feng Xdoi
10.1016/j.micpath.2012.02.001subject
Has Abstractpub_date
2012-05-01 00:00:00pages
267-77issue
5eissn
0882-4010issn
1096-1208pii
S0882-4010(12)00020-4journal_volume
52pub_type
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journal_title:Microbial pathogenesis
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2008.08.007
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journal_title:Microbial pathogenesis
pub_type: 杂志文章,meta分析
doi:10.1016/j.micpath.2012.05.007
更新日期:2012-08-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
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更新日期:1992-06-01 00:00:00
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更新日期:2017-11-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1996.0088
更新日期:1997-01-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1994.1013
更新日期:1994-02-01 00:00:00
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journal_title:Microbial pathogenesis
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doi:10.1016/j.micpath.2019.103693
更新日期:2019-11-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1016/j.micpath.2016.06.033
更新日期:2016-09-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.2001.0456
更新日期:2001-09-01 00:00:00
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journal_title:Microbial pathogenesis
pub_type: 杂志文章
doi:10.1006/mpat.1998.0241
更新日期:1999-01-01 00:00:00
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doi:10.1016/s0882-4010(03)00152-9
更新日期:2003-12-01 00:00:00
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更新日期:2012-02-01 00:00:00
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doi:10.1006/mpat.2001.0479
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journal_title:Microbial pathogenesis
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abstract::We have described a new class of live attenuated salmonella vaccines harbouring lesions in htrA, a stress protein gene previously. The virulence and invasiveness of Salmonella htrA mutants was investigated in three models of increased susceptibility to Salmonella infection. These included BALB/c mice, either given sub...
journal_title:Microbial pathogenesis
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doi:10.1016/0882-4010(92)90049-t
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journal_title:Microbial pathogenesis
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doi:10.1016/j.micpath.2012.10.002
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