Optical imaging of cancer heterogeneity with multispectral optoacoustic tomography.

Abstract:

PURPOSE:To investigate whether multispectral optoacoustic tomography (MSOT) can reveal the heterogeneous distributions of exogenous agents of interest and vascular characteristics through tumors of several millimeters in diameter in vivo. MATERIALS AND METHODS:Procedures involving animals were approved by the government of Upper Bavaria. Imaging of subcutaneous tumors in mice was performed by using an experimental MSOT setup that produces transverse images at 10 frames per second with an in-plane resolution of approximately 150 μm. To study dynamic contrast enhancement, three mice with 4T1 tumors were imaged before and immediately, 20 minutes, 4 hours, and 24 hours after systemic injection of indocyanine green (ICG). Epifluorescence imaging was used for comparison. MSOT of a targeted fluorescent agent (6 hours after injection) and hemoglobin oxygenation was performed simultaneously (4T1 tumors: n = 3). Epifluorescence of cryosections served as validation. The accumulation owing to enhanced permeability and retention in tumors (4T1 tumors: n = 4, HT29 tumors: n = 3, A2780 tumors: n = 2) was evaluated with use of long-circulating gold nanorods (before and immediately, 1 hour, 5 hours, and 24 hours after injection). Dark-field microscopy was used for validation. RESULTS:Dynamic contrast enhancement with ICG was possible. MSOT, in contrast to epifluorescence imaging, showed a heterogeneous intratumoral agent distribution. Simultaneous imaging of a targeted fluorescent agent and oxy- and deoxyhemoglobin gave functional information about tumor vasculature in addition to the related agent uptake. The accumulation of gold nanorods in tumors seen at MSOT over time also showed heterogeneous uptake. CONCLUSION:MSOT enables live high-spatial-resolution observations through tumors, producing images of distributions of fluorochromes and nanoparticles as well as tumor vasculature.

journal_name

Radiology

journal_title

Radiology

authors

Herzog E,Taruttis A,Beziere N,Lutich AA,Razansky D,Ntziachristos V

doi

10.1148/radiol.11111646

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

461-8

issue

2

eissn

0033-8419

issn

1527-1315

pii

263/2/461

journal_volume

263

pub_type

杂志文章
  • Reference values for diagnostic radiology: application and impact.

    abstract::Reference values (RVs) are recommended by the American Association of Physicists in Medicine for four radiographic projections, computed tomography, fluoroscopy, and dental radiography. RVs are used to compare radiation doses from individual pieces of radiographic equipment with doses from similar equipment assessed i...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.2352020016

    authors: Gray JE,Archer BR,Butler PF,Hobbs BB,Mettler FA Jr,Pizzutiello RJ Jr,Schueler BA,Strauss KJ,Suleiman OH,Yaffe MJ

    更新日期:2005-05-01 00:00:00

  • MR imaging of the knee. Part I. Traumatic disorders.

    abstract::One hundred thirty patients with a diversity of knee joint injuries were evaluated with a high-resolution magnetic resonance (MR) imaging technique. The authors report the accuracy of this technique in the evaluation of 105 patients with suspected meniscal tears, 26 patients with suspected cruciate ligament tears, and...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.162.2.3797670

    authors: Reicher MA,Hartzman S,Bassett LW,Mandelbaum B,Duckwiler G,Gold RH

    更新日期:1987-02-01 00:00:00

  • Autoimmune lymphoproliferative syndrome: a syndrome associated with inherited genetic defects that impair lymphocytic apoptosis--CT and US features.

    abstract:PURPOSE:To describe the imaging findings in patients with autoimmune lymphoproliferative syndrome (ALPS) and to relate the findings to the clinical and genetic features of this recently recognized syndrome. MATERIALS AND METHODS:Retrospective or prospective reviews of the computed tomographic (CT) and ultrasonographic...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.212.1.r99jl40257

    authors: Avila NA,Dwyer AJ,Dale JK,Lopatin UA,Sneller MC,Jaffe ES,Puck JM,Straus SE

    更新日期:1999-07-01 00:00:00

  • Prognostic value of grading masticator space involvement in nasopharyngeal carcinoma according to MR imaging findings.

    abstract:PURPOSE:To derive a suitable method for grading masticator space invasion in nasopharyngeal carcinoma on the basis of magnetic resonance (MR) images and to determine its prognostic value in patients undergoing intensity-modulated radiation therapy. MATERIALS AND METHODS:After institutional review board approval and in...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.14132745

    authors: Zhang GY,Huang Y,Cai XY,Chen XP,Xu T,Wu J,Wei WH,Liu LZ,Huang ZL,Zhou MM,Xia ST,Wang YJ

    更新日期:2014-10-01 00:00:00

  • Asymptomatic and neurologically symptomatic HIV-seropositive subjects: results of long-term MR imaging and clinical follow-up.

    abstract::Cranial magnetic resonance (MR) images were prospectively obtained in 74 human immunodeficiency virus (HIV)-seropositive subjects, all of whom had undergone MR imaging 24-42 months earlier. The images were interpreted by neuroradiologists blinded to the subject's clinical status and were compared with the initial MR i...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.188.3.8351340

    authors: Post MJ,Berger JR,Duncan R,Quencer RM,Pall L,Winfield D

    更新日期:1993-09-01 00:00:00

  • Radiographic chest abnormalities in adult hemophilia.

    abstract::The chest radiographs of 33 adult hemophiliacs were reviewed for possible pulmonary features of this disease. Only 7 patients showed no abnormalities; 12 patients exhibited scarring, fibrosis, and pleural thickening of the lung, interpreted as the sequelae of intrapulmonary hemorrhage or hemothorax. The remaining 14 p...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/118.1.41

    authors: Putman CE,Gamsu G,Zinn D,McLoud T

    更新日期:1976-01-01 00:00:00

  • Chest radiography: a survey of techniques and exposure levels currently used.

    abstract::In diagnostic radiology, the routine measurement of exposure levels for a reference patient is an important part of an effective quality assurance program. In the United States, chest radiography is the most frequent examination and has the lowest exposure level of all radiologic examinations. We estimated the amount ...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.156.2.4011920

    authors: Butler PF,Conway BJ,Suleiman OH,Koustenis GH,Showalter CK

    更新日期:1985-08-01 00:00:00

  • Pulmonary Hypertension: A Nomogram Based on CT Pulmonary Angiographic Data for Prediction in Patients without Pulmonary Embolism.

    abstract:PURPOSE:To use cardiovascular data from computerized tomographic (CT) pulmonary angiography for facilitating the identification of pulmonary hypertension (PH) in patients without acute pulmonary embolism. MATERIALS AND METHODS:The institutional human research committee approved this retrospective study; informed conse...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.15141269

    authors: Aviram G,Shmueli H,Adam SZ,Bendet A,Ziv-Baran T,Steinvil A,Berliner AS,Nesher N,Ben-Gal Y,Topilsky Y

    更新日期:2015-10-01 00:00:00

  • Performing thoracic transforaminal injections: a new technique.

    abstract:PURPOSE:To evaluate a technique to perform fluoroscopically directed thoracic transforaminal epidural injections. MATERIALS AND METHODS:Institutional review board approval and a waiver of consent were obtained for this retrospective HIPAA-compliant study. Findings from 198 consecutively performed foraminal nerve block...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.09082085

    authors: Rosas HG,Gilula LA

    更新日期:2010-02-01 00:00:00

  • Aortobronchial fistula as a complication of surgery for correction of congenital aortic anomalies.

    abstract::An aortobronchial fistula is a rare condition that is invariably fatal if not diagnosed and surgically treated. Aortic aneurysms, both atherosclerotic and infective, were the main causes in the originally described cases. However, thoracic cardiovascular surgery has recently become the predominant cause. The authors d...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.175.2.2326460

    authors: Garniek A,Morag B,Schmahmann S,Rubinstein ZJ

    更新日期:1990-05-01 00:00:00

  • Staging non-small cell lung cancer with whole-body PET.

    abstract:PURPOSE:To compare the accuracies of whole-body 2-[fluorine 18]fluoro-2-deoxy-D-glucose (FDG) positron emission tomography (PET) and conventional imaging (thoracic computed tomography [CT], bone scintigraphy, and brain CT or magnetic resonance [MR] imaging) in staging bronchogenic carcinoma. MATERIALS AND METHODS:With...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.212.3.r99se21803

    authors: Marom EM,McAdams HP,Erasmus JJ,Goodman PC,Culhane DK,Coleman RE,Herndon JE,Patz EF Jr

    更新日期:1999-09-01 00:00:00

  • Systemic hypocalcemia after clinical injections of radiographic contrast media: amelioration by omission of calcium chelating agents.

    abstract::Intravascular injection of some radiographic contrast media causes a fall in the concentration of unbound serum calcium (Ca++) and an increase in serum immunoreactive parathyroid hormone (iPTH). The decrease in Ca++ levels was attributed to the presence in the contrast media of calcium chelating agents (disodium edeta...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.147.3.6405450

    authors: Mallette LE,Gomez LS

    更新日期:1983-06-01 00:00:00

  • Application of region-of-interest imaging techniques to neurointerventional radiology.

    abstract::To reduce radiation exposure to patients and staff during neurointerventional procedures, region-of-interest (ROI) techniques were used with fluoroscopy, road mapping, and digital subtraction angiography in 19 patients. ROI filters, made of multiple layers of gadolinium, were attached to the collimators. Patient skin ...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.199.3.8638020

    authors: Rudin S,Guterman LR,Granger WE,Bednarek DR,Hopkins LN

    更新日期:1996-06-01 00:00:00

  • MR cholangiography: clinical evaluation in 40 cases.

    abstract:PURPOSE:To determine the reliability and efficacy of magnetic resonance (MR) cholangiography in demonstrating the site and extent of stricture. MATERIALS AND METHODS:In 40 patients with obstructive jaundice, three-dimensional MR cholangiography was performed with a sequence of mirrored fast imaging with steady-state p...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.189.2.8210370

    authors: Hall-Craggs MA,Allen CM,Owens CM,Theis BA,Donald JJ,Paley M,Wilkinson ID,Chong WK,Hatfield AR,Lees WR

    更新日期:1993-11-01 00:00:00

  • Time Course of Lung Changes at Chest CT during Recovery from Coronavirus Disease 2019 (COVID-19).

    abstract::Background Chest CT is used to assess the severity of lung involvement in coronavirus disease 2019 (COVID-19). Purpose To determine the changes in chest CT findings associated with COVID-19 from initial diagnosis until patient recovery. Materials and Methods This retrospective review included patients with real-time p...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.2020200370

    authors: Pan F,Ye T,Sun P,Gui S,Liang B,Li L,Zheng D,Wang J,Hesketh RL,Yang L,Zheng C

    更新日期:2020-06-01 00:00:00

  • Computers in radiology. A question of compatibility.

    abstract::Given the increasing diversification of computers in most areas of radiology, the ability to solve problems resulting from incompatibility between systems is becoming essential. Manufacturers should supply hardware and software documentation to allow connections between different systems and give the radiology departm...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.139.2.7220900

    authors: Ackerman LV

    更新日期:1981-05-01 00:00:00

  • Is There an Association between STARD Statement Adherence and Citation Rate?

    abstract::Purpose To determine if adherence to the Standards for Reporting of Diagnostic Accuracy (STARD) is associated with postpublication citation rates. Materials and Methods A comprehensive search of PubMed, EMBASE, and Cochrane Library databases was performed to identify published articles that have evaluated adherence of...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.2016151384

    authors: Dilauro M,McInnes MD,Korevaar DA,van der Pol CB,Petrcich W,Walther S,Quon J,Kurowecki D,Bossuyt PM

    更新日期:2016-07-01 00:00:00

  • Bronchial collapsibility at forced expiration in healthy volunteers: assessment with multidetector CT.

    abstract:PURPOSE:To assess forced-expiratory bronchial collapsibility in healthy volunteers by using multidetector computed tomography (CT) and to compare the results with the current diagnostic criterion for bronchomalacia. MATERIALS AND METHODS:The institutional review board approved this HIPAA-compliant study. Following inf...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.10100219

    authors: Litmanovich D,O'Donnell CR,Bankier AA,Ernst A,Millett ME,Loring SH,Boiselle PM

    更新日期:2010-11-01 00:00:00

  • Screening for occlusive vascular disease with intraarterial DSA: preliminary experience with a high flow 4-F catheter.

    abstract::Forty-two patients underwent intraarterial digital subtraction angiography (DSA) of the carotid or renal arteries, utilizing a new 4-F aortic flush catheter; 37 procedures were percutaneous transbrachial, five were percutaneous transfemoral. Image quality was consistently good or excellent. The safety of nonselective ...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.153.3.6387800

    authors: Becker GJ,Hicks ME,Holden RW,Edwards MK,Jackson VP,Bendick PJ

    更新日期:1984-12-01 00:00:00

  • Complications of arthrography.

    abstract::Arthrography is considered extremely safe since reactions to intraarticular contrast media are rare. We have only seen one severe complication in the performance of more than 2,000 arthrographic procedures. To determine the incidence of complications, a questionnaire was sent to 84 radiologists experienced in arthrogr...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.155.3.4001360

    authors: Newberg AH,Munn CS,Robbins AH

    更新日期:1985-06-01 00:00:00

  • CT predictors for differentiating benign and clinically worrisome pneumatosis intestinalis in children beyond the neonatal period.

    abstract:PURPOSE:To determine whether computed tomographic (CT) findings can help differentiate between benign and clinically worrisome causes of pneumatosis intestinalis (PI) in children. MATERIALS AND METHODS:This retrospective study was approved by the institutional review board, and requirement for informed consent was wai...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.2532090168

    authors: Olson DE,Kim YW,Ying J,Donnelly LF

    更新日期:2009-11-01 00:00:00

  • Dynamic contrast-enhanced subtraction MR angiography of the lower extremities: initial evaluation with a multisection two-dimensional time-of-flight sequence.

    abstract:PURPOSE:To determine the clinical feasibility of a contrast material--enhanced two-dimensional (2D) magnetic resonance (MR) angiographic sequence in lower extremity arteries. MATERIALS AND METHODS:Four healthy volunteers and 12 patients underwent dynamic contrast-enhanced MR angiography; all patients also underwent co...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.196.3.7644630

    authors: Adamis MK,Li W,Wielopolski PA,Kim D,Sax EJ,Kent KC,Edelman RR

    更新日期:1995-09-01 00:00:00

  • Gaucher disease: sonographic appearance of the spleen.

    abstract::Ultrasonographic (US) examinations of the upper abdomen were performed in 80 patients with Gaucher disease. Of the 49 patients that had not undergone splenectomy, 47 had splenic enlargement. Sixteen patients had multiple lesions in the spleen. Most patients had discrete hypoechoic lesions that corresponded pathologica...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.160.3.3526400

    authors: Hill SC,Reinig JW,Barranger JA,Fink J,Shawker TH

    更新日期:1986-09-01 00:00:00

  • Functional and morphologic evaluation of congenital urinary tract dilatation by using combined static-dynamic MR urography: findings in kidneys with a single collecting system.

    abstract:PURPOSE:To assess combined static-dynamic magnetic resonance (MR) urography in the evaluation of congenital urinary tract dilatation in infants and children. MATERIALS AND METHODS:Sixty-two patients with urinary tract dilatation underwent prospective examination with combined static-dynamic MR urography. A combination...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiol.2243011207

    authors: Rohrschneider WK,Haufe S,Wiesel M,Tönshoff B,Wunsch R,Darge K,Clorius JH,Tröger J

    更新日期:2002-09-01 00:00:00

  • Infrapopliteal percutaneous transluminal angioplasty: what we know.

    abstract::We believe that a substantial experience demonstrating the effectiveness and safety of infrapopliteal artery PTA has been accumulated. It is clear that the results of tibial PTA and femoropopliteal PTA are closely associated for most patients undergoing limb salvage procedures. Anatomic selection is most important; pa...

    journal_title:Radiology

    pub_type: 评论,杂志文章,评审

    doi:10.1148/radiology.200.1.8657940

    authors: Bakal CW,Cynamon J,Sprayregen S

    更新日期:1996-07-01 00:00:00

  • Rectosigmoid pseudostenosis due to urinary retention.

    abstract::Pseudostenosis of the rectosigmoid colon due to compression by a dilated urinary bladder may pose a diagnostic problem. Although probably quite common, it has not received much attention in the radiological literature. Four cases are presented and the radiological features on barium enema are described. ...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/127.3.645

    authors: Kleinhaus U,Kaftori J

    更新日期:1978-06-01 00:00:00

  • Core-needle and surgical breast biopsy: comparison of three methods of assessing cost.

    abstract:PURPOSE:To compare and evaluate the measures of costs for core-needle and surgical breast biopsies. MATERIALS AND METHODS:Three measures of costs were evaluated: (a) input resources, (b) actual payments, and (c) billed charges. A combination of methods were used for data collection from 10 sites enrolled in a large-sc...

    journal_title:Radiology

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1148/radiology.212.1.r99jl46181

    authors: Burkhardt JH,Sunshine JH

    更新日期:1999-07-01 00:00:00

  • Prostate cancer: sextant localization at MR imaging and MR spectroscopic imaging before prostatectomy--results of ACRIN prospective multi-institutional clinicopathologic study.

    abstract:PURPOSE:To determine the incremental benefit of combined endorectal magnetic resonance (MR) imaging and MR spectroscopic imaging, as compared with endorectal MR imaging alone, for sextant localization of peripheral zone (PZ) prostate cancer. MATERIALS AND METHODS:This prospective multicenter study, conducted by the Am...

    journal_title:Radiology

    pub_type: 杂志文章,多中心研究

    doi:10.1148/radiol.2511080409

    authors: Weinreb JC,Blume JD,Coakley FV,Wheeler TM,Cormack JB,Sotto CK,Cho H,Kawashima A,Tempany-Afdhal CM,Macura KJ,Rosen M,Gerst SR,Kurhanewicz J

    更新日期:2009-04-01 00:00:00

  • Detection of varicocele by radionuclide blood-pool scanning.

    abstract::Varicocele is a common and treatable cause of male subfertility. The authors describe a new technique for varicocele detection using radionuclide blood-pool imaging of the scrotum. Of 83 patients studied, 29 had a clinically palpable varicocele of various sizes, and 26 of them were demonstrated by this technique. Two ...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/radiology.137.1.7422850

    authors: Freund J,Handelsman DJ,Bautovich GJ,Conway AJ,Morris JG

    更新日期:1980-10-01 00:00:00

  • Computed tomographic cavo-urography: lower-extremity contrast infusion simultaneous with computed tomography of the retroperitoneum.

    abstract::Successful opacification and distention of the inferior vena cava was obtained simultaneously with computed tomography by contrast infusion through a lower-extremity vein. Images obtained by this method displayed retroperitoneal anatomical relationships to better advantage, permitting more accurate interpretation of t...

    journal_title:Radiology

    pub_type: 杂志文章

    doi:10.1148/130.3.797

    authors: Pillari G

    更新日期:1979-03-01 00:00:00