Abstract：
:It is generally accepted that fungi have a number of dormant gene clusters for the synthesis of secondary metabolites, and the activation of these gene clusters can expand the diversity of secondary metabolites in culture. Recent studies have revealed that the mycolic acid-containing bacterium Tsukamurella pulmonis activates dormant gene clusters in the bacterial genus Streptomyces. However, it is not clear whether the mycolic acid-containing bacteria activate dormant gene clusters of fungi. We performed co-culture experiments using marine-derived Aspergillus niger with Mycobacterium smegmatis, a mycolic acid-containing bacteria. The co-cultivation resulted in the production of a pigment by A. niger and increased cytotoxic activity of the extract against human prostate cancer DU145 cells. An analysis of secondary metabolites in the extract of the co-culture broth revealed that the increase in cytotoxic activity was caused by the production of malformin C (1), and that TMC-256A1 (2), desmethylkotanin (3), and aurasperone C (4) were selectively produced under co-culture conditions. In addition, further study suggested that direct interaction between the two microorganisms was necessary for the production of the pigment and the cytotoxic compound malformin C (1) from A. niger. Given the biological activities of malformin C, including cytotoxic activity, our approach for increasing the production of bioactive secondary metabolites has important practical applications and may facilitate structural analyses of novel bioactive compounds.
journal_name
J Nat Medjournal_title
Journal of natural medicinesauthors
Jomori T,Hara Y,Sasaoka M,Harada K,Setiawan A,Hirata K,Kimishima A,Arai Mdoi
10.1007/s11418-019-01345-0subject
Has Abstractpub_date
2020-01-01 00:00:00pages
76-82issue
1eissn
1340-3443issn
1861-0293pii
10.1007/s11418-019-01345-0journal_volume
74pub_type
杂志文章abstract：:The evaluation of the antioxidant, anti-secretory and anti-inflammatory potentials of the ethyl acetate fraction (ELC) from the root bark of Lycium chinense against ethanol-induced gastric ulcer in mice and the possible mechanisms underlying this action was performed. The results indicated that oral administration of ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-016-0984-2
更新日期：2016-07-01 00:00:00
abstract：:This investigation was aimed to probe the pharmacological base of medicinal use of Acorus calamus in ischemic heart diseases. Effect on heart parameters was studied in isolated rabbit heart while coronary vasodilator effect was studied in isolated bovine coronary arterial rings, suspended in tissue baths filled with K...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0561-7
更新日期：2012-01-01 00:00:00
abstract：:Acanthopanax senticosus HARMS (AS) is used as a Chinese herbal medicine and as a health supplement in Japan. However, little is known about the interaction between AS and other drugs. In this study, we investigated the effect of AS extract on intestinal drug transporter (P-glycoprotein, or P-gp) and peptide transporte...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-009-0371-3
更新日期：2010-01-01 00:00:00
abstract：:The predominant feature of type 2 diabetes is insulin resistance. Identifying a drug able to reduce insulin resistance is an urgent requirement. ent-3α-Formylabieta-8(14),13(15)-dien-16,12β-olide had been identified as a new diterpene derivative which showed anticancer activity. This study explores the hypoglycemic ef...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-019-01384-7
更新日期：2020-03-01 00:00:00
abstract：:This study reports on the determination of 11 elements in 33 medicinal plants from Sudan and discusses a possible correlation between their curative effects and their trace elements content. Further, a possible accumulation of adverse heavy metals could be excluded. A total of 11 elements (cadmium, lead, mercury, tin,...
journal_title：Journal of natural medicines
pub_type： 信件
doi：10.1007/s11418-012-0630-6
更新日期：2012-10-01 00:00:00
abstract：:A new oxygenated lanostane-type triterpene, named lucidumol C, together with six known compounds, was isolated from the chloroform extract of the fruiting bodies of Ganoderma lingzhi. Structures were established based on extensive spectroscopic and chemical studies. Potential cytotoxic activities of the isolated compo...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-016-0976-2
更新日期：2016-07-01 00:00:00
abstract：:Advanced glycation end-products (AGEs) are associated with a non-enzymatic reaction between the amino group of a protein and the carbonyl group of a sugar during hyperglycemia. The precipitation of AGEs in different tissues leads to many complications, such as endothelial dysfunction, cardiovascular complications, ath...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-016-1051-8
更新日期：2017-01-01 00:00:00
abstract：:A new phenylethanoid, 1-O-β-D-(4-hydroxyphenyl)-ethyl-6-O-vanilloyl-glucopyranoside (1), and a new furofuran lignan, (7R,7'R,7''S,8S,8'S,8''R)-4', 4''-dihydroxy-3,3',3'',5-tetramethoxy-7,9':7'9-diepoxy-4,8''-oxy-8,8'-sesquineolignan-7'',9''-diol (2), along with five known compounds (3-7) were isolated from the roots o...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-016-0996-y
更新日期：2016-07-01 00:00:00
abstract：:A new aromatic derivative, 5-(3,4-dihydroxyphenyl)-3-methylcyclohex-2-enone (1), and three known compounds, peltigerine, solorinine, and phenyl butanoid, were isolated from the MeOH extract of the lichen Peltigera dolichorrhiza (Peltigeraceae). Their structures were determined by 1D and 2D nuclear magnetic resonance a...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-010-0409-6
更新日期：2010-07-01 00:00:00
abstract：:The core of solid tumors is characterized by hypoxia and a nutrient-starved microenvironment and has gained much attention as targets of anti-cancer drugs. In the course of search for selective growth inhibitors against the cancer cells adapted to nutrient starvation, epidithiodiketopiperazine DC1149B (1) together wit...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-019-01357-w
更新日期：2020-01-01 00:00:00
abstract：:Picrorhiza kurrooa Royle ex Benth (Scrophulariaceae), commonly known as Kutki, is a major ingredient of many ayurvedic preparations prescribed in the treatment of various diseases. Picrosides I and II are the active agents responsible for the medicinal effects of Kutki, and the variation in content of these compounds ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-010-0491-9
更新日期：2011-07-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) possesses two distinct enzymatic activities: those of RNA- and DNA-dependent DNA polymerases and RNase H. In the current HIV-1 therapy, all HIV-1 RT inhibitors inhibit the activity of DNA polymerase, but not that of RNase H. We previously reported ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-015-0885-9
更新日期：2015-07-01 00:00:00
abstract：:Activity-guided isolation of the n-butanol fraction of Chamaerhodos erecta and water soluble fraction of C. altaica resulted in the isolation of 39 compounds, including new compounds identified as 4,5-dihydroxybenzaldehyde-3-O-β-D-glucopyranoside (1) from C. erecta and quercetin-3-O-β-D-glucuronopyranosyl-4'-O-β-D-glu...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-013-0748-1
更新日期：2013-10-01 00:00:00
abstract：:(E)-methyl-4-hydroxy-3,5-dimethoxycinnamate, (E)-methyl-4-hydroxy-3-methoxycinnamate, (E)-methyl-4-hydroxycinnamate, (Z)-methyl-4-hydroxycinnamate, calycanthine, ursolic acid, 2-hydroxy-3-methylanthraquinone, 4-methoxybenzoic acid, 3,4,5-trimethoxybenzoic acid, methyl 4-hydroxybenzoate and allantoin were isolated from...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-008-0227-2
更新日期：2008-07-01 00:00:00
abstract：:Tetrandrine is a cytotoxic compound capable of exerting remarkable antitumor activity against many cancer cells in vitro and in vivo. However, little is known about its effect on human renal cell carcinoma (RCC). In the present study, using RCC 786-O, 769-P and ACHN cell lines as the model system, we demonstrated the ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-013-0765-0
更新日期：2014-01-01 00:00:00
abstract：:Three new acetylated C-glycosylflavones, 3″,6″-di-O-acetylswertiajaponin (1), 4″,6″-di-O-acetylswertiajaponin (2), and 6″-O-acetylswertiajaponin (3), together with six known compounds were isolated from the whole herb of Swertia mileensis. Their structures were elucidated on extensive NMR experiments and mass spectrom...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1225-7
更新日期：2018-09-01 00:00:00
abstract：:In recent years, Spirulina has gained more and more attention from medical scientists as a nutraceutical and a source of potential pharmaceuticals. The present study was conducted to elucidate the immunomodulatory effect of Spirulina fusiformis (a cyanobacterium of the family Oscillatoriaceae) in vivo and in vitro. Th...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-008-0308-2
更新日期：2009-04-01 00:00:00
abstract：:Atherosclerosis is a major cause of coronary heart disease. As a result of the development of atherosclerotic lesions, the walls of blood vessels become thicker and inhibit blood circulation. Atherosclerosis is caused by a high-fat diet and vascular injury. Chronic arterial inflammation plays an important role in the ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1231-9
更新日期：2019-01-01 00:00:00
abstract：:A new pyrrolidine derivative, (5S)-hydroxyethyl 2-oxopyrrolidine-5-carboxylate (1), a new flavonol glycoside, tamaraxetin 3,7-di-O-α-L-rhamnopyranoside (2), and a new triterpene saponin, polyscioside A methyl ester (3), along with six known compounds (4-9) were isolated from the leaves of Polyscias balfouriana. Their ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-017-1081-x
更新日期：2017-07-01 00:00:00
abstract：:Six cycloartane glycosides, named tareciliosides N-S, and a known iridoid glucoside, ixoroside, were isolated in further extensive studies on the 1-BuOH-soluble fraction of a MeOH extract of the leaves of Tarenna gracilipes. From the EtOAc-soluble fraction, three known triterpenes were isolated. Their toxicities towar...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0707-2
更新日期：2013-07-01 00:00:00
abstract：:Near-infrared (NIR) spectroscopy combined with chemometrics has been utilized in predictions of natural medicine content without destroying samples. Suppositories (oren powdered extract content 0, 0.5, 1.0, 2.5, 10, 12.5, and 15%) were produced by mixing oren powdered extract with macrogol mixture consisting of 1 part...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0596-9
更新日期：2012-04-01 00:00:00
abstract：:Ancient Chinese medicine treatises on Atractylodis Macrocephalae Rhizoma (AMR), the rhizome of Atractylodes macrocephala Koidz, indicated that it possessed an expectorant effect. However, in modern times, it is commonly used as a tocolytic agent. In this study, the components of AMR that are responsible for its expect...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-015-0934-4
更新日期：2016-01-01 00:00:00
abstract：:I investigated the causative agents of licorice-induced pseudoaldosteronism, which is a frequent side effect of Japanese traditional Kampo medicines. Glycyrrhizin (GL), the main ingredient of licorice, is absorbed after being metabolized to glycyrrhetinic acid (GA) by intestinal bacteria, and then metabolized in liver...
journal_title：Journal of natural medicines
pub_type： 杂志文章,评审
doi：10.1007/s11418-021-01484-3
更新日期：2021-01-22 00:00:00
abstract：:Shakuyakukanzoto (SKT) is a kampo medicine composed of equal proportions of Glycyrrhizae radix (G. radix) and Paeoniae radix (P. radix). A double-blind study reported that SKT significantly ameliorated painful muscle cramp in cirrhosis patients without the typical severe side effects of muscle weakness and central ner...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-015-0890-z
更新日期：2015-07-01 00:00:00
abstract：:From the 1-BuOH-soluble fraction of a MeOH extract of Euphorbia hirta, collected in the Okinawa islands, three new megastigmane glucoside gallates, named hirtionosides A-C, 3-hydroxyoctanoic acid glucosides and a phenylpropanoid glucoside were isolated along with 15 known compounds. The structures of the new compounds...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0692-5
更新日期：2013-04-01 00:00:00
abstract：:We investigated the cytotoxicity of six neolignans (1-6), which are similar in structure to furanocyclohexenone with an angular allyl group, in human neuroblastoma cell lines (IMR-32, LA-N-1, NB-39, SK-N-SH) and normal cells [human umbilical vein endothelial cells (HUVEC) and human dermal fibroblasts (HDF)] using a 3-...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0514-1
更新日期：2011-07-01 00:00:00
abstract：:Evodiae fructus (EF), a fruit of Evodia rutaecarpa Bentham, has long been used as an analgesic drug in traditional Chinese and Japanese medicine. However, the underlying molecular mechanism of its pharmacological action is unclear. Here, using calcium imaging, whole-cell patch-clamp recording, and behavioral analysis,...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-015-0929-1
更新日期：2016-01-01 00:00:00
abstract：:In a previous study, we found that both synthetic 3-O-methylquercetin (3MQ) and 3,4',7-O-trimethylquercetin (34'7TMQ) increased extracellular melanin content. 34'7TMQ increased the activity of melanogenic enzymes by stimulating the p38 pathway and the expression of microphthalmia-associated transcription factor (MITF)...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1175-0
更新日期：2018-03-01 00:00:00
abstract：:The root of Angelica acutiloba Kitagawa is an important crude drug in Kampo medicines (traditional Japanese medicine). Chemical evaluation of crude drugs is crucial to ensuring the safety and efficacy of herbal medicine; however, there is currently no chemical standard for the A. acutiloba crude drug in Japanese pharm...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1190-1
更新日期：2018-06-01 00:00:00
abstract：:Smallanthus sonchifolius (yacon) is a perennial plant mostly cultivated in South America, primarily for use of the tubers as a food crop and the leaves as fodder for livestock. The antibacterial activities of the methanol extract of yacon leaves (S. sonchifolius) and its n-hexane, ethyl acetate, n-butanol and water fr...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-010-0388-7
更新日期：2010-04-01 00:00:00
