In vitro metabolism of cyadox in rat, chicken and swine using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry.

Abstract:

:In this work, metabolites of cyadox (CYX) produced by liver microsomes, primary hepatocytes and intestinal microflora systems of rat, chicken and swine were identified and elucidated using ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS). Expected and unexpected metabolites were found out using Metabolynx(XS) software by automatically comparing MS(E) data from the sample and control. The metabolites were reliably characterized by accurate MS/MS spectra and their different fragmentation pathways. In total twenty-four metabolites were identified in the three in vitro systems, fifteen of which were novel. The results revealed the main metabolic pathways of CYX were N→O group reduction, hydroxylation and hydrolysis reactions. Besides, methylation and acetylation reactions which represented phase II drug biotransformations were recognized in the in vitro systems of chicken firstly. It also showed that the metabolic capacity and rapidity of chicken and swine was greater than rat, with some differences in metabolic fate and metabolite varieties. This work contributes to the comprehensive understanding of in vitro metabolism of CYX, and will provide an important basis for further in vivo metabolism study and toxicological safety evaluation.

journal_name

J Pharm Biomed Anal

authors

Wu H,Li L,Shen J,Wang Y,Liu K,Zhang S

doi

10.1016/j.jpba.2012.04.004

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

175-85

eissn

0731-7085

issn

1873-264X

pii

S0731-7085(12)00201-4

journal_volume

67-68

pub_type

杂志文章
  • Estimation of impurity profiles of drugs and related materials. Part 9: HPLC investigation of flumecinol.

    abstract::Two HPLC systems have been developed for the investigation of flumecinol (3-trifluoromethyl-alpha-ethyl-benzhydrol). The reversed-phase system (LiChrosorb RP-18; methanol-water, 7:3, v/v) enables the isolation and identification of the impurities. The chiral system (Chiralcel OD; hexane-2-propanol, 98:2, v/v) separate...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/0731-7085(91)80088-q

    authors: Görög S,Herényi B,Rényei M

    更新日期:1992-10-01 00:00:00

  • Serum levels of beta-trace protein and glomerular filtration rate--preliminary results.

    abstract::The aim of this study was to evaluate the relationship between serum levels of beta-trace protein (BTP), a low molecular weight (MW) protein, and glomerular filtration rate (GFR). GFR and serum levels of BTP, and for comparison creatinine (Creat), cystatin C (Cys) and beta 2-microglobulin (beta 2M), were measured in 6...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(03)00215-2

    authors: Donadio C,Lucchesi A,Ardini M,Donadio E,Giordani R

    更新日期:2003-08-08 00:00:00

  • The quality of sildenafil active substance of illegal source.

    abstract::There must be a large market for active pharmaceutical ingredients of illegal source to support the huge and lucrative business of trade in illegal medicines. The active substances found in illegal pharmaceuticals may differ from their legal counterparts concerning purity and associated risks for the health of the use...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2016.08.027

    authors: Keizers PHJ,Wiegard A,Venhuis BJ

    更新日期:2016-11-30 00:00:00

  • Flavor analysis in a pharmaceutical oral solution formulation using an electronic-nose.

    abstract::Flavors are commonly used in pharmaceutical oral solutions and oral suspensions to mask drug bitterness and to make the formulation more palatable. Flavor analysis during product development is typically performed by human organoleptic analysis, which is often expensive and less objective. A novel approach using a met...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(03)00651-4

    authors: Zhu L,Seburg RA,Tsai E,Puech S,Mifsud JC

    更新日期:2004-02-18 00:00:00

  • A simple method for assaying colistimethate sodium in pharmaceutical aerosol samples using high performance liquid chromatography.

    abstract::A rapid and simple reversed-phase high performance liquid chromatography (HPLC) method for the quantitation of colistimethate sodium in pharmaceutical formulations has been developed. The chromatographic separation was performed using a Phenomenex Kinetex XB-C18 column with gradient elution using a mobile phase contai...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2017.04.021

    authors: Metcalf AP,Hardaker LEA,Hatley RHM

    更新日期:2017-08-05 00:00:00

  • Preconcentration and determination of ultra-traces of platinum in human serum using the combined electrodeposition-electrothermal atomic absorption spectroscopy (ED-ETAAS) and chemometric method.

    abstract::Platinum compounds, including cis-dichlorodiaminoplatinum(II) or cisplatin, are an important class of anti-cancer drugs, which should be carefully monitored in the biological fluids. In this study, electrodeposition coupled with electrothermal atomic absorption spectrometry (ETAAS) was used for determination of Pt con...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2010.02.023

    authors: Najafi NM,Shahparvizi S,Rafati H,Ghasemi E,Alizadeh R

    更新日期:2010-09-21 00:00:00

  • Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging.

    abstract::Health benefits of wine, especially with red wine, have been linked to the presence of a wide range of phenolic antioxidants. Thus, the aim of this study was to develop a simple, high performance thin layer chromatographic (HPTLC) method combined with high resolution digital plate images to visually compare multiple w...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2014.08.031

    authors: Agatonovic-Kustrin S,Hettiarachchi CG,Morton DW,Razic S

    更新日期:2015-01-01 00:00:00

  • Analysis of phosphodiesterase type 5 inhibitors as possible adulterants of botanical-based dietary supplements: extensive survey of preparations available at the Czech market.

    abstract::Popularity of natural-based preparations supporting the sexual potency significantly increased in recent years, which also led to the increase of illegal use of phosphodiesterase type 5 inhibitor (PDE-5) in sexual performance enhancement products. In this study, a rapid U-HPLC‒HRMS/MS method has been developed to simu...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2018.11.007

    authors: Jiru M,Stranska-Zachariasova M,Dzuman Z,Hurkova K,Tomaniova M,Stepan R,Cuhra P,Hajslova J

    更新日期:2019-02-05 00:00:00

  • Simultaneous determination of lamotrigine, phenobarbitone, carbamazepine and phenytoin in human serum by high-performance liquid chromatography.

    abstract::A simple reversed-phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous estimation of the antiepileptic drugs (AEDs) lamotrigine (LTG), phenobarbitone (PB), carbamazepine (CBZ) and phenytoin (PHT) in human serum. The procedure involves extraction of the AEDs by mixing 200 microl...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2005.02.045

    authors: Patil KM,Bodhankar SL

    更新日期:2005-09-01 00:00:00

  • Application of a self-modeling curve resolution method for studying the photodegradation kinetics of nitrendipine and felodipine.

    abstract::Dihydropyridine (DHP) derivatives, as calcium channel blockers with cardiovascular activity, are highly photosensitive and converted in the presence of light to compounds that are inactive. In this work, a self-modeling curve resolution method was applied to study the photodegradation kinetics of nitrendipine and felo...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2007.11.020

    authors: Javidnia K,Hemmateenejad B,Miri R,Saeidi-Boroujeni M

    更新日期:2008-02-13 00:00:00

  • Microwave-assisted extraction for Hibiscus sabdariffa bioactive compounds.

    abstract::H. sabdariffa has demonstrated positive results against chronic diseases due to the presence of phytochemicals, mainly phenolic compounds. The extraction process of bioactive compounds increases the efficient collection of extracts with high bioactivity. Microwave-Assisted Extraction (MAE) constituted a "green technol...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2018.04.050

    authors: Pimentel-Moral S,Borrás-Linares I,Lozano-Sánchez J,Arráez-Román D,Martínez-Férez A,Segura-Carretero A

    更新日期:2018-07-15 00:00:00

  • Assay of terfenadine in pharmaceutical formulation and human plasma by adsorptive stripping voltammetry.

    abstract::The controlled adsorptive accumulation of Zn(II)-terfenadine complex (1:1) onto a hanging mercury drop electrode (HMDE) provides the basis for determination of the antihistamine drug terfenadine by differential pulse cathodic adsorptive stripping voltammetry. The adsorbed Zn(II)-terfenadine complex (1:1) at the HMDE d...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(01)00491-5

    authors: Ghoneim M,Issa R,Tawfik A

    更新日期:2001-11-01 00:00:00

  • Comparison of reversed phase and reversed phase ion pair high performance liquid chromatography for analysis of TcO and TcN complexes of L,L-ethylene dicysteine di-ethylester and its acid analogues.

    abstract::99mTc(V)-oxo complexes of L,L-ethylene dicysteine (L,L-EC) and its di-ester derivative L,L-ethyl cysteinate dimer (L,L-ECD) are useful tracer agents for evaluation of renal function and cerebral blood flow respectively. Labelling of these molecules with a 99mTc(V)-nitrido core instead of the 99mTc(V)-oxo core alters t...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(96)01736-0

    authors: Mang'era KO,Bellande E,Pasqualini R,Verbruggen A

    更新日期:1996-06-01 00:00:00

  • Difference spectrophotometric assay of 5-hydroxymethylfurfuraldehyde in hydrolysed pharmaceutical syrups-II. Isoniazid reagent.

    abstract::A rapid difference spectrophotometric assay of 5-hydroxymethylfurfuraldehyde (5-HMF) in certain degraded syrups is described. The method involves the measurement of the difference absorbance at 340 nm of the isonicotinoyl hydrazone of 5-HMF, formed at room temperature in an acidic solution of isoniazid, relative to an...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/0731-7085(88)80030-x

    authors: Davidson AG,Dawodu TO

    更新日期:1988-01-01 00:00:00

  • Isolation and characterization of a tadalafil analogue, N-cyclopentyl nortadalafil in health supplement.

    abstract::A tadalafil analogue was detected for the first time during the screening of a health supplement for undeclared sexual enhancement drugs. The compound had been isolated and purified by preparative high-pressure liquid chromatography (HPLC). Its chemical structure was elucidated using high-resolution mass spectrometry ...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2015.08.005

    authors: Xu Y,Kee CL,Ge X,Low MY,Koh HL

    更新日期:2016-01-25 00:00:00

  • The validation of column-switching LC/MS as a high-throughput approach for direct analysis of di(2-ethylhexyl) phthalate released from PVC medical devices in intravenous solution.

    abstract::Health Canada reported recently that medical devices containing di(2-ethylhexyl) phthalate (DEHP) should not be used in the clinical treatment of infants, young boys, pregnant women, and nursing mothers. The risk assessment of DEHP released from PVC medical devices is an important issue for hospitalized patients. In t...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(03)00019-0

    authors: Inoue K,Higuchi T,Okada F,Iguchi H,Yoshimura Y,Sato A,Nakazawa H

    更新日期:2003-04-10 00:00:00

  • Rutin determination in pharmaceutical formulations using a carbon paste electrode modified with poly(vinylpyrrolidone).

    abstract::A carbon paste electrode modified with poly(vinylpyrrolidone) (PVP) was developed for the determination of rutin. PVP enhances the adsorption of rutin on the electrode surface due to the presence of hydrogen bonding between the imide group in PVP and the hydroxyl group in rutin. The current responses in cyclic and lin...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2008.03.031

    authors: Franzoi AC,Spinelli A,Vieira IC

    更新日期:2008-08-05 00:00:00

  • Development and application of bio-sample quantification to evaluate stability and pharmacokinetics of inulin-type fructo-oligosaccharides from Morinda Officinalis.

    abstract::Inulin-type fructooligosaccharides (FOS) purified from Morinda Officinalis, with degrees of polymerization (DP) from 3 to 9, have been approved in China as an oral prescribed drug for mild and moderate depression episode, while the stability and oral absorption of this FOS mixtures are largely unknown. As the main act...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2018.04.028

    authors: Chi L,Chen L,Zhang J,Zhao J,Li S,Zheng Y

    更新日期:2018-07-15 00:00:00

  • Model-based strategy for bioanalytical method comparison: measurement of a soluble ligand as a biomarker.

    abstract::Ligand binding assays (LBAs) are often the method of choice for quantification of protein biomarkers and therapeutic biologics during drug development. Soluble ligand X is a glycoprotein. To understand the role of circulating ligand X in drug-target relationship, an analytical method (Method 1) was developed and valid...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2011.09.004

    authors: Thway TM,Wang J,Brunner LA,Gu W,Magana I,Padbury G,Ma M

    更新日期:2012-01-25 00:00:00

  • A simple and rapid assay for heparanase activity using homogeneous time-resolved fluorescence.

    abstract::Degradation of heparan sulfate proteoglycan by heparanase is an important process in tissue invasion by malignant tumor cells and inflammatory cells. Several heparanase assays have been reported previously, but all of them are cumbersome and time-consuming for separating degradative products from uncleaved substrates ...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2006.01.032

    authors: Enomoto K,Okamoto H,Numata Y,Takemoto H

    更新日期:2006-06-07 00:00:00

  • A quantitative LC-MS/MS method for simultaneous determination of cocaine and its metabolites in whole blood.

    abstract::As new metabolic pathways of cocaine were recently identified, a high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine cocaine and nine cocaine-related metabolites in whole blood samples. One-step solid phase extraction was used to extract all of th...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2016.11.024

    authors: Chen X,Zheng X,Ding K,Zhou Z,Zhan CG,Zheng F

    更新日期:2017-02-05 00:00:00

  • Pharmaceutical impurities and degradation products: uses and applications of NMR techniques.

    abstract::Current standards and regulations demand the pharmaceutical industry not only to produce highly pure drug substances, but to achieve a thorough understanding of the impurities accompanying their manufactured drug substances and products. These challenges have become important goals of process chemistry and have steadi...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章,评审

    doi:10.1016/j.jpba.2014.04.016

    authors: Maggio RM,Calvo NL,Vignaduzzo SE,Kaufman TS

    更新日期:2014-12-01 00:00:00

  • Analytical methods for assay of ellagic acid and its solubility studies.

    abstract::Ultra-violet (UV) spectrophotometric and high performance liquid chromatographic methods for quantitative determination of ellagic acid (EA), an antioxidant were developed. The analytical methods were validated for linearity, accuracy, intra- and inter-day variability, and precision. EA was eluted using a polyethylene...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2005.07.006

    authors: Bala I,Bhardwaj V,Hariharan S,Kumar MN

    更新日期:2006-01-23 00:00:00

  • Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using ¹H NMR spectroscopy and multivariate statistical analysis.

    abstract::Puerariae Radix was a widely used herbal medicine. Pueraria lobata (PL) and Pueraria thomsonii (PT) were the two authorized sources of Puerariae Radix (gegen) in China. In this study, metabolic differentiations between these two species were investigated using NMR spectroscopy followed by principal components analysis...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2013.05.017

    authors: Chen YG,Song YL,Wang Y,Yuan YF,Huang XJ,Ye WC,Wang YT,Zhang QW

    更新日期:2014-05-01 00:00:00

  • Application of quality by design elements for the development and optimization of an analytical method for protamine sulfate.

    abstract::The purpose of this study was to develop a robust reverse phase-HPLC method for the separation of hydrolyzed protamine sulfate peptides using a quality by design approach. A Plackett-Burman experimental design was utilized to screen the effects of mobile phase pH, flow rate, column temperature, injection volume and me...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2012.01.002

    authors: Awotwe-Otoo D,Agarabi C,Faustino PJ,Habib MJ,Lee S,Khan MA,Shah RB

    更新日期:2012-03-25 00:00:00

  • Identification of reaction products between drug substances and excipients by HPLC-SPE-NMR: ester and amide formation between citric acid and 5-aminosalicylic acid.

    abstract::The reaction between the high-dose drug substance 5-aminosalicylic acid (5-ASA) and the excipient citric acid during storage of an experimental enema preparation has been studied and three isobaric reaction products, i.e., an ester and an amide with non-symmetrically substituted citric acid moieties and a symmetrical ...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2008.12.028

    authors: Larsen J,Staerk D,Cornett C,Hansen SH,Jaroszewski JW

    更新日期:2009-04-05 00:00:00

  • Underlying assumptions in the estimation of secondary structure content in proteins by circular dichroism spectroscopy--a critical review.

    abstract::Recombinant DNA technology has made possible the large-scale production of proteins for pharmaceutical applications. As a result, there has been a renaissance in methodology which can provide information on the structural stability and character of these materials. Circular dichroism (CD) spectroscopy, with its sensit...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章,评审

    doi:10.1016/0731-7085(89)80049-4

    authors: Manning MC

    更新日期:1989-01-01 00:00:00

  • Development and validation of LC-MS/MS method for the detection and quantification of CpG oligonucleotides 107 (CpG ODN107) and its metabolites in mice plasma.

    abstract::CpG oligodeoxynucleotide 107 (CpG ODN107) could be used as a novel radiosensitizer for glioma. Herein, a novel and sensitive reversed-phase HPLC coupled with electrospray triple quadrupole mass spectrometry (LC-MS/MS) following a one-step C18 solid-phase extraction (SPE) for biological matrix removal was developed and...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2012.06.022

    authors: Cen Y,Li X,Liu D,Pan F,Cai Y,Li B,Peng W,Wu C,Jiang W,Zhou H

    更新日期:2012-11-01 00:00:00

  • Estimation of anthelmintic compound 81/470 in cow's milk by high-performance liquid chromatography: method development and validation.

    abstract::CDRI compound 81/470 is a new broad spectrum anthelmintic agent and is being developed for veterinary use. HPLC assay method for 81/470 in cow milk was developed and validated. The sample preparation consisted of protein precipitation, followed by extraction with ether. Separation was achieved on a C18 column using ac...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(96)01913-9

    authors: Nagaraja NV,Singh SK,Gupta RC

    更新日期:1997-05-01 00:00:00

  • Quantitation of uridine and L-dihydroorotic acid in human plasma by LC-MS/MS using a surrogate matrix approach.

    abstract::Uridine and L-dihydroorotate (DHO) are important intermediates of de novo as well as salvage pathways for the biosynthesis of pyrimidines, which are the building blocks of nucleic acids - DNA and RNA. These metabolites are known to be significant biomarkers of pyrimidine synthesis during the development of DHODH inhib...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2020.113669

    authors: Yin F,Ling Y,Martin J,Narayanaswamy R,McIntosh L,Li F,Liu G

    更新日期:2021-01-05 00:00:00