Abstract：
Background:The mixed-cultivation of different Panax ginseng cultivars can cause adverse effects on stability of yield and quality. K-1 is a superior cultivar with good root shape and stronger disease resistance. DNA markers mined from functional genes are clearly desirable for K-1, as they may associate with major traits and can be used for marker-assisted selection to maintain the high quality of Korean ginseng. Methods:Five genes encoding pathogenesis-related (PR) proteins of P. ginseng were amplified and compared for polymorphism mining. Primary, secondary, and tertiary structures of PR5 protein were analyzed by ExPASy-ProtParam, PSSpred, and I-TASSER methods, respectively. A coding single nucleotide polymorphism (SNP)-based specific primer was designed for K-1 by introducing a destabilizing mismatch within the 3' end. Allele-specific polymerase chain reaction (PCR) and real-time allele-specific PCR assays were conducted for molecular discrimination of K-1 from other cultivars and landraces. Results:A coding SNP leading to the modification of amino acid residue from aspartic acid to asparagine was exploited in PR5 gene of K-1 cultivar. Bioinformatics analysis showed that the modification of amino acid residue changed the secondary and tertiary structures of the PR5 protein. Primer KSR was designed for specific discrimination of K-1 from other ginseng cultivars and landraces. The developed real-time allele-specific PCR assay enabled easier automation and accurate genotyping of K-1 from a large number of ginseng samples. Conclusion:The SNP marker and the developed real-time allele-specific PCR assay will be useful not only for marker-assisted selection of K-1 cultivar but also for quality control in breeding and seed programs of P. ginseng.
journal_name
J Ginseng Resjournal_title
Journal of ginseng researchauthors
Wang H,Xu F,Wang X,Kwon WS,Yang DCdoi
10.1016/j.jgr.2018.07.001subject
Has Abstractpub_date
2019-07-01 00:00:00pages
482-487issue
3eissn
1226-8453issn
2093-4947pii
S1226-8453(18)30118-0journal_volume
43pub_type
杂志文章abstract：Background:Panax ginseng Meyer has been used as a nourishing edible herb in East Asia for thousands of years. 25-OH-PPT was first discovered as a natural rare triterpenoid saponin in ginseng stems and leaves by our group. Research found that it showed strong inhibitory effects on α-glucosidase and protein tyrosine phos...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2019.11.002
更新日期：2021-01-01 00:00:00
abstract：:Ginsenoside (ginseng saponin), the principal component of ginseng, is responsible for the pharmacological and biological activities of ginseng. We isolated lactic acid bacteria from Kimchi using esculin agar, to produce β-glucosidase. We focused on the bio-transformation of ginsenoside. Phylogenetic analysis was perfo...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.5142/jgr.2012.36.3.291
更新日期：2012-07-01 00:00:00
abstract：:The detrimental impact of air pollution as a result of frequent exposure to fine particles posed a global public health risk mainly to the pulmonary disorders in pediatric and geriatric population. Here, we reviewed the current literature regarding the role of ginseng and/or its components as antimicrobials, especiall...
journal_title：Journal of ginseng research
pub_type： 杂志文章,评审
doi：10.1016/j.jgr.2019.12.001
更新日期：2020-03-01 00:00:00
abstract：:Ginseng has been used as an herbal medicine, widely used in Asian countries, for long time. Recently, beneficial effects for immune functions of Korean red ginseng (KRG) have been reported in adults. This study was performed to investigate the effects of ginseng on immune functions in children after cessation of chemo...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.5142/jgr.2012.36.4.383
更新日期：2012-10-01 00:00:00
abstract：Background:Korean ginseng (Panax ginseng) plays an anti-inflammatory role in a variety of inflammatory diseases such as gastritis, hepatitis, and colitis. However, inflammation-regulatory activity of the calyx of the P. ginseng berry has not been thoroughly evaluated. To understand whether the calyx portion of the P. g...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2017.06.003
更新日期：2018-10-01 00:00:00
abstract：:Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.5142/jgr.2011.35.3.352
更新日期：2011-09-01 00:00:00
abstract：Background:Ginsenoside Rh2 (GRh2) is a characterized component in red ginseng widely used in Korea and China. GRh2 exhibits a wide range of pharmacological activities, such as anti-inflammatory, antioxidant, and anticancer properties. However, its effects on Toxoplasma gondii (T. gondii) infection have not been clarifi...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2019.06.002
更新日期：2020-09-01 00:00:00
abstract：:In this study, photosynthetic parameters such as the net photosynthesis rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate were examined in selected ginseng varieties and/or lines that are resistant (Yunpoong, HTIR 1, HTIR 2, and HTIR 3) and susceptible (Chunpoong) to high temperature ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.5142/jgr.2012.36.4.461
更新日期：2012-10-01 00:00:00
abstract：Background:The root and rhizome are historically and officially utilized medicinal parts of Panax notoginseng (PN) (Burk.) F. H. Chen, which in raw and steamed forms are used differently in practice. Methods:To investigate the differences in chemical composition and bioactivities of PN root and rhizome between raw and...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2017.11.004
更新日期：2019-07-01 00:00:00
abstract：BACKGROUND:We evaluated the drug interaction profile of Red Ginseng (RG) with respect to the activities of major cytochrome P450 (CYP) enzymes and the drug transporter P-glycoprotein (P-gp) in healthy Korean volunteers. METHODS:This article describes an open-label, crossover study. CYP probe cocktail drugs, caffeine, ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2015.11.005
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND:Minor saponins or human intestinal bacterial metabolites, such as ginsenosides Rg3, F2, Rh2, and compound K, are more pharmacologically active than major saponins, such as ginsenosides Rb1, Rb2, and Rc. In this work, enzymatic hydrolysis of ginsenoside Rb1 was studied using enzyme preparations from cultured ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2015.05.007
更新日期：2016-04-01 00:00:00
abstract：BACKGROUND:Steaming of ginseng is known to change its chemical composition and biological activity. This study was carried out to investigate the effect of different steaming time-scales on chemical constituents and antiproliferative activity of Vietnamese ginseng (VG). METHODS:VG was steamed at 105°C for 2-20 h. Its ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2015.01.006
更新日期：2015-07-01 00:00:00
abstract：:Jilin Province is one of the principal production bases of traditional Chinese medicine (TCM) in China with its typical preponderance in TCM resources, research and development power, and industrialization capacity. The province has 2,790 species of TCM materials in total. Over 20% of the TCM materials in common use a...
journal_title：Journal of ginseng research
pub_type： 杂志文章,评审
doi：10.1016/j.jgr.2014.05.007
更新日期：2014-10-15 00:00:00
abstract：Background:The incidence of colorectal cancer (CRC) is increasing, with metastasis of newly diagnosed CRC reported in a large proportion of patients. However, the effect of Korean Red Ginseng extracts (KRGE) on epithelial to mesenchymal transition (EMT) in CRC is unknown. Therefore, we examined the mechanisms by which ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2017.03.008
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:Although the aerial parts of hydroponic Panax ginseng are reported to contain higher contents of total ginsenosides than those of roots, the isolation and identification of active metabolites from the aerial parts of hydroponic P. ginseng have not been carried out so far. METHODS:The aerial parts of hydropo...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2014.10.005
更新日期：2015-04-01 00:00:00
abstract：:We previously reported that in vitro anti-platelet activity of tissue-cultured mountain ginseng (TCMG) ethanol extracts show improved efficacy when compared with commercial ginseng products such as Korean red ginseng and Panax ginseng. However, information on the anti-platelet activity of the ethyl acetate fraction fr...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.5142/jgr.2011.35.4.442
更新日期：2011-11-01 00:00:00
abstract：Background:Depression is a common neuropsychiatric disease that shows astrocyte pathology. Ginsenoside Rf (G-Rf) is a saponin found in Panax ginseng which has been used to treat neuropsychiatric diseases. We aimed to investigate antidepressant properties of G-Rf when introduced into the L-alpha-aminoadipic acid (L-AAA)...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2019.08.005
更新日期：2020-07-01 00:00:00
abstract：:Longevity in medicine can be defined as a long life without mental or physical deficits. This can be prevented by Alzheimer's disease (AD). Current conventional AD treatments only alleviate the symptoms without reversing AD progression. Recent studies demonstrated that Panax ginseng extract improves AD symptoms in pat...
journal_title：Journal of ginseng research
pub_type： 杂志文章,评审
doi：10.1016/j.jgr.2017.12.008
更新日期：2018-10-01 00:00:00
abstract：Background:Ginsenoside Rf is a ginseng saponin found only in Panax ginseng that affects lipid metabolism. It also has neuroprotective and antiinflammatory properties. We previously showed that Korean Red Ginseng (KRG) inhibited the expression of cyclooxygenase-2 (COX-2) by hypoxia via peroxisome proliferator-activated ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2018.11.007
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:The current typical drying methods for red ginseng are sun drying and hot-air drying. The purpose of this study was to investigate drying characteristics of red ginseng by using far-infrared drying. METHODS:The far-infrared drying tests on red ginseng were conducted at two drying stages: (1) high temperatur...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2015.04.001
更新日期：2015-10-01 00:00:00
abstract：Background:Ginsenoside Rg3, a derivative of steroidal saponins abundant in ginseng, has a range of effects on cancer cells, including anti-cell proliferation and anti-inflammation activity. Here, we investigate two long noncoding RNAs (lncRNAs), STXBP5-AS1 and RFX3-AS1, which are hypomethylated and hypermethylated in t...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2019.02.004
更新日期：2019-10-01 00:00:00
abstract：:An light-emitting diode (LED)-based light source was used as a monochromatic light source to determine the responses of raw ginseng roots (Panax ginseng Meyer) to specific emission spectra with respect to the production of ginsenosides. The ginsenoside content in the ginseng roots changed in response to the LED light ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.5142/jgr.2012.36.2.198
更新日期：2012-04-01 00:00:00
abstract：BACKGROUND:Roles of immune reaction and toll-like receptor-4 (TLR-4) have widely been established in the pathogenesis of alcoholic liver disease (ALD). METHODS:We evaluated the biologic efficacy of Korean Red Ginseng (KRG), urushiol, and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) in...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2014.04.002
更新日期：2014-07-01 00:00:00
abstract：Background:Recently, we identified a novel ginseng-derived lysophosphatidic acid receptor ligand, called gintonin. We showed that gintonin induces [Ca2+]i transient-mediated morphological changes, proliferation, and migration in cells expressing lysophosphatidic acid receptors and that oral administration of gintonin e...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2016.12.007
更新日期：2018-01-01 00:00:00
abstract：Background:Ginseng is a commonly used herbal medicine in treating various medical conditions. Chronic gut inflammation is a recognized factor for the development of colorectal cancer (CRC). In this project, Asian ginseng berry polysaccharide preparations were used to assess their effects on CRC and related immune regul...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2018.12.010
更新日期：2020-03-01 00:00:00
abstract：Background:The circadian rhythm is the internal clock that controls sleep-wake cycles, metabolism, cognition, and several processes in the body, and its disruption has been associated with aging. The differentiated embryo chondrocyte (Dec) gene is related to circadian rhythm. To our knowledge, there are no reports of t...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2020.09.003
更新日期：2021-01-01 00:00:00
abstract：:There seems to be some controversy about the effect of total ginseng saponin (TGS) on the secretion of catecholamines (CA) from the adrenal gland. Therefore, the present study aimed to determine whether TGS can affect the CA release in the perfused model of the adrenal medulla isolated from spontaneously hypertensive ...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.5142/jgr.2011.35.2.176
更新日期：2011-06-01 00:00:00
abstract：Background:Ginsenosides, which are bioactive components in ginseng, can be converted to smaller compounds for improvement of their pharmacological activities. The conversion methods include heating; acid, alkali, and enzymatic treatment; and microbial conversion. The aim of this study was to determine the bioconversion...
journal_title：Journal of ginseng research
pub_type： 杂志文章
doi：10.1016/j.jgr.2017.07.004
更新日期：2019-01-01 00:00:00
abstract：:Ginseng, a perennial plant belonging to the genus Panax of the Araliaceae family, is well known for its medicinal properties that help alleviate pathological symptoms, promote health, and prevent potential diseases. Among the active ingredients of ginseng are saponins, most of which are glycosides of triterpenoid agly...
journal_title：Journal of ginseng research
pub_type： 杂志文章,评审
doi：10.1016/j.jgr.2014.12.005
更新日期：2015-10-01 00:00:00
abstract：:The ginseng plant (Panax ginseng Meyer) has a large number of active ingredients including steroidal saponins with a dammarane skeleton as well as protopanaxadiol and protopanaxatriol, commonly known as ginsenosides, which have antioxidant, anticancer, antidiabetic, anti-adipocyte, and sexual enhancing effects. Though...
journal_title：Journal of ginseng research
pub_type： 杂志文章,评审
doi：10.5142/jgr.2013.37.261
更新日期：2013-07-01 00:00:00
