Abstract:
:The length-tension (L-T) relationships in rabbit detrusor smooth muscle (DSM) are similar to those in vascular and airway smooth muscles and exhibit short-term length adaptation characterized by L-T curves that shift along the length axis as a function of activation and strain history. In contrast to skeletal muscle, the length-active tension (L-T(a)) curve for rabbit DSM strips does not have a unique peak tension value with a single ascending and descending limb. Instead, DSM can exhibit multiple ascending and descending limbs, and repeated KCl-induced contractions at a particular muscle length on an ascending or descending limb display increasingly greater tension. In the present study, mouse bladder strips with and without urothelium exhibited KCl-induced and carbachol-induced length adaptation, and the pressure-volume relationship in mouse whole bladder displayed short-term volume adaptation. Finally, prostaglandin-E(2)-induced low-level rhythmic contraction produced length adaptation in rabbit DSM strips. A likely role of length adaptation during bladder filling is to prepare DSM cells to contract efficiently over a broad range of volumes. Mammalian bladders exhibit spontaneous rhythmic contraction (SRC) during the filling phase and SRC is elevated in humans with overactive bladder (OAB). The present data identify a potential physiological role for SRC in bladder adaptation and motivate the investigation of a potential link between short-term volume adaptation and OAB with impaired contractility.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Speich JE,Wilson CW,Almasri AM,Southern JB,Klausner AP,Ratz PHdoi
10.1007/s10439-012-0590-8subject
Has Abstractpub_date
2012-10-01 00:00:00pages
2266-76issue
10eissn
0090-6964issn
1573-9686journal_volume
40pub_type
杂志文章abstract::Förster resonance energy transfer (FRET) technology has been widely used in biological and biomedical research and is a very powerful tool in elucidating protein interactions in many cellular processes. Ubiquitination and SUMOylation are multi-step cascade reactions, involving multiple enzymes and protein-protein inte...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-010-0225-x
更新日期:2011-04-01 00:00:00
abstract::The objective of this study was to develop a ferrocene mediated glucose biosensor for reverse iontophoresis. An amperometric ferrocene mediated glucose biosensor based on a three electrodes planar configuration was constructed using screen printing technique. Different combinations of glucose oxidase and ferrocene loa...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-010-9918-4
更新日期:2010-04-01 00:00:00
abstract::This study was aimed at developing a physical model, supported by experimental observations, to describe the formation and growth of microbubbles seen in patients with mitral mechanical heart valves (MHV). This phenomenon, often referred to as high intensity transient signals (HITS), appears as bright, intense, high-v...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.231
更新日期:1999-11-01 00:00:00
abstract::In 12 dogs anesthetised with alpha-chloralose and urethane, and beta-adrenergic blocked with propranolol, the cervical vagi were stimulated for 60 seconds with supramaximal constant current pulses at frequencies between 2.0 and 10 Hz. The time course of the recovery of the cardiac period response, after cessation of v...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02584303
更新日期:1991-01-01 00:00:00
abstract::In this paper, we aim to establish a new mathematical model that relates pulse wave velocity (PWV) to blood pressure (BP) for continuous and noninvasive BP measurement. For the first time, we derive an ordinary differential equation (ODE) expressing the fundamental relation between BP, elastic modulus G and PWV. The g...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-009-9759-1
更新日期:2009-11-01 00:00:00
abstract::An understanding of the mechanical deformation behavior of the liver under high strain rate loading conditions could aid in the development of vehicle safety measures to reduce the occurrence of blunt liver injury. The purpose of this study was to develop a constitutive model of the stress-strain behavior of the human...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-008-9555-3
更新日期:2008-11-01 00:00:00
abstract::Cell migration paths of mammary epithelial cells (expressing different versions of the promigratory tyrosine kinase receptor Her2/Neu) were analyzed within a bimodal framework that is a generalization of the run-and-tumble description applicable to bacterial migration. The mammalian cell trajectories were segregated i...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-008-9592-y
更新日期:2009-01-01 00:00:00
abstract::The induction of angiogenesis is a promising therapeutic strategy for the amelioration of peripheral arterial disease (PAD). This occlusive disease results in muscle ischemia, and neovascularization is a route to increasing the perfusion in the tissue. The vascular endothelial growth factor (VEGF) family of potent pro...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-007-9303-0
更新日期:2007-06-01 00:00:00
abstract::Conventional nanoporous hydrogels often lead to slow cartilage deposition by MSCs in 3D due to physical constraints and requirement for degradation. Our group has recently reported macroporous gelatin microribbon (μRB) hydrogels, which substantially accelerate MSC-based cartilage formation in vitro compared to convent...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-020-02522-z
更新日期:2020-07-01 00:00:00
abstract::A novel electrocardiogram (ECG) electrode film is developed by mixing carbon black powder and a quaternary salt with a visco-elastic polymeric adhesive. Unlike traditional wet gel-based electrodes, carbon/salt/adhesive (CSA) electrodes should theoretically have an infinite shelf life as they do not dehydrate even afte...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-015-1282-y
更新日期:2015-10-01 00:00:00
abstract::Upon nerve injury, the body creates an environment consisting of permissive and non-permissive cues that instruct the function of cells involved in nerve repair. Among other roles, the developing extracellular matrix (ECM) acts as an underlying substrate to guide the union of neurites extending from the proximal stump...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-014-0991-y
更新日期:2014-06-01 00:00:00
abstract::Twenty-one 3.5-month-old female Sprague-Dawley rats were randomly assigned to three groups: BTX group, in which each rat received a single intramuscular injection of 2 U of Clostridium botulinum toxin (BTX) in the quadriceps femoris muscle of the right hind limb; BTX + SR group, in which each rat received a BTX inject...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-011-0406-2
更新日期:2012-03-01 00:00:00
abstract::It is widely recognized that the bacteria sequestered in a biofilm on a medical implant are much more resistant to antibiotics than their planktonic counterparts. Recent studies have shown that application of antibiotic along with low power ultrasound significantly increases the killing of planktonic bacteria by the a...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02738539
更新日期:1997-01-01 00:00:00
abstract::The planar fibrous connective tissues of the body are composed of a dense extracellular network of collagen and elastin fibers embedded in a ground matrix, and thus can be thought of as biocomposites. Thus, the quantification of fiber architecture is an important step in developing an understanding of the mechanics of...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02684845
更新日期:1997-07-01 00:00:00
abstract::Although diagnostic testing with auditory evoked potentials (EPs) has become routine, quantitative measurements of signal and noise are still lacking. In this study, current signal, power, noise power, and signal-to-noise ratio (SNR) estimation formulas are reviewed and applied to auditory brainstem response averaging...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02684183
更新日期:1996-11-01 00:00:00
abstract::We propose a multiphysical mathematical model by fully coupling lipid deposition, monocytes/macrophages recruitment and angiogenesis to investigate the pathophysiological responses of an atherosclerotic plaque to the dynamic changes in the microenvironment. The time evolutions of cellular (endothelial cells, macrophag...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-018-02173-1
更新日期:2019-02-01 00:00:00
abstract::There are recent reports of hybrid tissue-fabric materials with good performance-high biocompatibility and high mechanical strength. In this study, we demonstrate the capability of a hybrid material as a long-term filter for blood proteins. Polyester fabrics were implanted into rats to fabricate hybrid tissue-fabric m...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-016-1781-5
更新日期:2017-05-01 00:00:00
abstract::Intracellular calcium concentration and transmembrane potentials are two important measurements used to study the mechanisms of cardiac activity. Fluorescent dyes have been used to measure these separately but not simultaneously in cardiac tissue. Fluo-3 and Fluo-4 (a recently improved version of Fluo-3) have been use...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.198
更新日期:1999-07-01 00:00:00
abstract::Gap junctions play a fundamental role in intercellular communication in cardiac tissue. Various types of heart disease including hypertrophy and ischemia are associated with alterations of the spatial arrangement of gap junctions. Previous studies applied two-dimensional optical and electron-microscopy to visualize ga...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-011-0369-3
更新日期:2011-11-01 00:00:00
abstract::A comparison was made between turbulence calculated by subtracting an ensemble average from the instantaneous velocity and calculations made with a high pass digital filter. Velocity was measured with a laser Doppler anemometer in vitro in the region of a normal porcine aortic valve and in patients with a hot film ane...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02584215
更新日期:1983-01-01 00:00:00
abstract::We attempted to estimate in living adherent epithelial alveolar cells, the degree of structural and mechanical heterogeneity by considering two individualized cytoskeleton components, i.e., a submembranous "cortical" cytoskeleton and a "deep" cytoskeleton (CSK). F-actin structure characterizing each CSK component was ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.1616932
更新日期:2003-11-01 00:00:00
abstract::A theoretical model is presented for describing the motion of a deformable cell encapsulating a Newtonian fluid and enclosed by an elastic membrane in tube flow. In the mathematical formulation, the interior and exterior hydrodynamics are coupled with the membrane mechanics by means of surface equilibrium equations, a...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-005-8975-6
更新日期:2005-02-01 00:00:00
abstract::The concept of body force (force per unit volume) is introduced to account for the effect of the force generated in the radial direction by the active state of the myocardium in an elastic model of the left ventricle represented as a thick-walled cylinder contracting symmetrically. Experimental evidence for the validi...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02368077
更新日期:1991-01-01 00:00:00
abstract::There are a number of cell therapies that are either in clinical trials or moving toward clinical trials, particularly for diseases of the retina. One of the challenges with cell therapies is tracking the status of cells over time. Genetic manipulation can facilitate this, but it can limit the clinical application of ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-019-02393-z
更新日期:2020-07-01 00:00:00
abstract::The long held assumption that sustained drug elution from stent coatings over weeks to months is imperative for clinical efficacy has limited the choice for stent coating materials. We developed and evaluated an omega-3 fatty acid (O3FA) based stent coating that is 85% absorbed and elutes 97% of its Sirolimus analog (...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-015-1435-z
更新日期:2016-02-01 00:00:00
abstract::An MR image-based computational model of a murine KHT sarcoma is presented that allows the calculation of plasma fluid and solute transport within tissue. Such image-based models of solid tumors may be used to optimize patient-specific therapies. This model incorporates heterogeneous vasculature and tissue porosity to...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-011-0349-7
更新日期:2011-09-01 00:00:00
abstract::Microstructural characterization of cardiac tissue and its remodeling in disease is a crucial step in many basic research projects. We present a comprehensive approach for three-dimensional characterization of cardiac tissue at the submicrometer scale. We developed a compression-free mounting method as well as labelin...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-015-1465-6
更新日期:2016-05-01 00:00:00
abstract::Analysis and classification of esophageal motility records were investigated using signal processing and fuzzy-set pattern recognition techniques. A set of parameters has been extracted from the raw records and has previously been used as characterizing features. Improvements to these procedures were obtained by extra...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02368227
更新日期:1994-01-01 00:00:00
abstract::High-frequency ventilation (HFV) has not one but several histories. Proceeding along largely independent pathways are techniques using frequencies of 1-5 Hz and techniques using 10-40 Hz, the former being introduced by Sjostrand in 1971, the latter by Lunkenheimer in 1972. It was nearly ten years before it was recogni...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02407779
更新日期:1984-01-01 00:00:00
abstract::The impedance of a monopolar electrode immersed in an environmental volume conductor consists of two parts; the impedance of the active electrode-electrolyte interface, and the resistance of the environmental conductor. Two studies were carried out to quantitate these components. First, impedance-frequency data were c...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02368609
更新日期:1992-01-01 00:00:00