Genotoxic effects of cisplatin in somatic tissue of Drosophila melanogaster.

Abstract:

:Third instar larvae of Drosophila melanogaster transdihybrid for mwh and flr were exposed to varying concentrations of cisplatin by feeding on dry media wetted with aqueous solutions of the test compound. Larval feeding continued until pupation, and surviving transdihybrid adults were collected seven days following commencement of feeding. Wings of adults were removed and scored under 400X magnification for the presence of twin spots and single spots comprised of clones of cells possessing malformed wing hairs. Cisplatin was found to induce both twin spots and single spots, and significant (p less than 0.05) linear concentration-response relationships were obtained with respect to the induction of all endpoints. Induction of twin spots demonstrates that cisplatin induces mitotic recombination in the somatic tissue of Drosophila larvae. This capacity to induce mitotic exchange in the somatic tissue of Drosophila compares well with the compound's reported ability to induce chromosome breaks in Drosophila germ cells [Brodberg et al. 1983]. However, not all compounds possess similar genotoxic profiles in the somatic and germ tissue of Drosophila.

journal_name

Environ Mol Mutagen

authors

Katz AJ

doi

10.1002/em.2850100210

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

197-203

issue

2

eissn

0893-6692

issn

1098-2280

journal_volume

10

pub_type

杂志文章
  • Salmonella mutagenicity tests: V. Results from the testing of 311 chemicals.

    abstract::311 chemicals were tested under code, for mutagenicity, in Salmonella typhimurium; 35 of the chemicals were tested more than once in the same or different laboratories. The tests were conducted using a preincubation protocol in the absence of exogenous metabolic activation, and in the presence of liver S-9 from Aroclo...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.2850190603

    authors: Zeiger E,Anderson B,Haworth S,Lawlor T,Mortelmans K

    更新日期:1992-01-01 00:00:00

  • Impact of transforming viruses on cellular mutagenesis, genome stability, and cellular transformation.

    abstract::It is estimated that 15% of all cancers are etiologically linked to viral infection. Specific cancers including adult T-cell leukemia, hepatocellular carcinoma, and uterine cervical cancer are associated with infection by human T-cell leukemia virus type I, hepatitis B virus, and high-risk human papilloma virus, respe...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,评审

    doi:10.1002/em.20088

    authors: Gatza ML,Chandhasin C,Ducu RI,Marriott SJ

    更新日期:2005-03-01 00:00:00

  • Looking backward on a century of mutation research.

    abstract::Our understanding of the mutation process and how it impacts target populations has deepened steadily during the hundred years since 1919. Recent advances in engineering genetically determined social traits points towards the logical culmination of this knowledge. ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 历史文章,杂志文章

    doi:10.1002/em.2850230605

    authors: Drake JW

    更新日期:1994-01-01 00:00:00

  • Telomere measurement in individuals occupationally exposed to pesticide mixtures in tobacco fields.

    abstract::Occupational exposure to pesticides in tobacco fields causes genetic damage in farmers. The aim of this study was to analyze tobacco farmers chronically exposed to low doses of pesticides and nicotine (present in the tobacco leaves) in relation to absolute telomere length (aTL), and explore the influence of lifestyle ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.21984

    authors: Kahl VF,Simon D,Salvador M,Branco Cdos S,Dias JF,da Silva FR,de Souza CT,da Silva J

    更新日期:2016-01-01 00:00:00

  • Epididymal sperm aneuploidies in three strains of rats detected by multicolor fluorescence in situ hybridization.

    abstract::A multicolor fluorescence in situ hybridization (FISH) method was developed to detect aneuploidy and diploidy in epididymal sperm of rats using DNA probes specific for chromosomes 4 and Y. Fourteen healthy young-adult rats from three strains were evaluated: inbred Fisher 344/N/ehs, outbred Sprague-Dawley, and outbred ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:

    authors: Lowe XR,de Stoppelaar JM,Bishop J,Cassel M,Hoebee B,Moore D 2nd,Wyrobek AJ

    更新日期:1998-01-01 00:00:00

  • Association between atmospheric ozone levels and damage to human nasal mucosa in Florence, Italy.

    abstract::We evaluated the effects of urban air pollutants on human nasal mucosa over an 8-month period on 102 subjects living in Florence, Tuscany, Italy. A group of subjects living in a city with a lower level of pollution (Sassari, Sardinia, Italy) was also analyzed. Nasal mucosa cells were harvested by brushing, a noninvasi...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.10188

    authors: Pacini S,Giovannelli L,Gulisano M,Peruzzi B,Polli G,Boddi V,Ruggiero M,Bozzo C,Stomeo F,Fenu G,Pezzatini S,Pitozzi V,Dolara P

    更新日期:2003-01-01 00:00:00

  • Apoptosis induction in human lung adenocarcinoma cells by oil-soluble allyl sulfides: triggers, pathways, and modulators.

    abstract::DAS (diallyl sulfide), DADS (diallyl disulfide), and DATS (diallyl trisulfide) are major oil-soluble allyl sulfides (OAS) that represent major garlic constituents. The anticarcinogenic and antimutagenic effects of these substances have been extensively studied during the last decades. Previous reports suggest that ind...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.20467

    authors: Wu XJ,Hu Y,Lamy E,Mersch-Sundermann V

    更新日期:2009-04-01 00:00:00

  • Evaluation of genetic damage in open-cast coal mine workers using the buccal micronucleus cytome assay.

    abstract::Coal is the largest fossil fuel source used for the generation of energy. However, coal extraction and its use constitute important pollution factors; thus, risk characterization and estimation are extremely important for the safety of coal workers and the environment. Candiota is located to the southeast of the state...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.21744

    authors: Rohr P,da Silva J,da Silva FR,Sarmento M,Porto C,Debastiani R,Dos Santos CE,Dias JF,Kvitko K

    更新日期:2013-01-01 00:00:00

  • Glycosylphosphatidylinositol (GPI) anchored protein deficiency serves as a reliable reporter of Pig-a gene Mutation: Support from an in vitro assay based on L5178Y/Tk+/- cells and the CD90.2 antigen.

    abstract::Lack of cell surface glycosylphosphatidylinositol (GPI)-anchored protein(s) has been used as a reporter of Pig-a gene mutation in several model systems. As an extension of this work, our laboratory initiated development of an in vitro mutation assay based on the flow cytometric assessment of CD90.2 expression on the c...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.22154

    authors: Bemis JC,Avlasevich SL,Labash C,McKinzie P,Revollo J,Dobrovolsky VN,Dertinger SD

    更新日期:2018-01-01 00:00:00

  • In vivo genotoxic effects of industrial waste leachates in mice following oral exposure.

    abstract::Contamination of ground water by industrial waste poses potential health hazards for man and his environment. The improper disposal of toxic wastes could allow genotoxic chemicals to percolate into ground waters, and these contaminated ground waters may produce toxicity, including mutation and eventually cancer, in ex...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.20210

    authors: Chandra S,Chauhan LK,Murthy RC,Gupta SK

    更新日期:2006-06-01 00:00:00

  • Irradiation of primary human gliomas triggers dynamic and aggressive survival responses involving microvesicle signaling.

    abstract::Malignant gliomas are heterogeneous populations of dynamically interacting cells. Genomic and transcriptional changes define this cellular hierarchy and allow certain tumor cells to co-opt metabolic machinery and adopt gene expression profiles that promote cellular reprogramming. Resultant expansion of privileged subp...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.21988

    authors: Baulch JE,Geidzinski E,Tran KK,Yu L,Zhou YH,Limoli CL

    更新日期:2016-06-01 00:00:00

  • Both PIGA and PIGL mutations cause GPI-a deficient isolates in the Tk6 cell line.

    abstract::Molecular analysis of proaerolysin selected glycosylphosphatidylinositol anchor (GPI-a) deficient isolates in the TK6 cell line was performed. Initial studies found that the expected X-linked PIGA mutations were rare among the spontaneous isolates but did increase modestly after ethyl methane sulfate (EMS) treatment (...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.21953

    authors: Nicklas JA,Carter EW,Albertini RJ

    更新日期:2015-10-01 00:00:00

  • Induction of micronuclei in human sperm-hamster egg hybrids at the two-cell stage after in vitro gamma-irradiation of human spermatozoa.

    abstract::The efficiency of the micronucleus test to assess radiation-induced chromosomal damage in human spermatozoa has been investigated. Micronuclei were scored in human sperm-hamster egg hybrids at the two-cell stage, after exposure of human spermatozoa to in vitro gamma-rays at doses of 0.00, 0.10, 0.25, 0.50, 1.00, 2.00,...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.2850260407

    authors: Tusell L,Alvarez R,Caballín MR,Genescà A,Miró R,Ribas M,Egozcue J

    更新日期:1995-01-01 00:00:00

  • Mutational spectra in transgenic animal research: data analysis and study design based upon the mutant or mutation frequency.

    abstract::Understanding chemically induced changes in mutational spectra can aid in deciphering mechanisms of mutagenesis. In this paper, we propose the use of statistical methods that are based upon the mutation frequency, rather than simple mutant counts which have no relationship to the mutation frequency. These methods have...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2280(1996)28:4<405::AID-EM15>3.

    authors: Carr GJ,Gorelick NJ

    更新日期:1996-01-01 00:00:00

  • Genetic toxicology testing of the antimalarial drugs chloroquine and a new analog, AQ-13.

    abstract::AQ-13 ([N1-(7-chloro-quinolin-4yl)-3-(N3,N3-diethylamino)propylamine] dihydrochloride trihydrate) is an aminoquinoline antimalarial drug that is effective against chloroquine-resistant strains of Plasmodium falciparum. It is structurally similar to the widely used chloroquine diphosphate (CQ). We evaluated these drugs...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.1052

    authors: Riccio ES,Lee PS,Winegar RA,Krogstad DJ,De D,Mirsalis JC

    更新日期:2001-01-01 00:00:00

  • The use of benchmark dose uncertainty measurements for robust comparative potency analyses.

    abstract::The Benchmark Dose (BMD) method is the favored approach for quantitative dose-response analysis where uncertainty measurements are delineated between the upper (BMDU) and lower (BMDL) confidence bounds, or confidence intervals (CIs). Little has been published on the accurate interpretation of uncertainty measurements ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.22422

    authors: Wheeldon RP,Dertinger SD,Bryce SM,Bemis JC,Johnson GE

    更新日期:2021-01-11 00:00:00

  • Mutagenicity and clastogenicity of gas stove emissions in bacterial and plant tests.

    abstract::The aim of this research was to study the gaseous and particulate emissions of genotoxic substances during cooking with two types of methane stoves (a new one and an old one). The particulates were sampled both with a cascade impactor air sampler and an impinger with ice trap and analyzed by two bacterial mutagenicity...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:

    authors: Monarca S,Zanardini A,Feretti D,Falistocco E,Antonelli P,Resola S,Moretti M,Villarini M,Nardi G

    更新日期:1998-01-01 00:00:00

  • Sources of variability in data from a lacI transgenic mouse mutation assay.

    abstract::Experimental features of a transgenic mouse mutation assay based on a lacI target transgene from Escherichia coli are considered in detail. Sources of variability in the experimental protocol that can affect the statistical nature of the observations are examined with the goal of identifying sources of excess variatio...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.2850230105

    authors: Piegorsch WW,Lockhart AM,Margolin BH,Tindall KR,Gorelick NJ,Short JM,Carr GJ,Thompson ED,Shelby MD

    更新日期:1994-01-01 00:00:00

  • Sister chromatid exchange and micronucleus frequency in human lymphocytes of 1,650 subjects in an Italian population: I. Contribution of methodological factors.

    abstract::The influence of several methodological factors on mean values of sister chromatid exchanges (SCEs) and micronuclei (MN) in peripheral lymphocytes of 1,650 subjects was analyzed. Donors belonged to a general healthy population living in Pisa and in two nearby small cities: Cascina and Navacchio (Ca-Na). Blood samples ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2280(1998)31:3<218::aid-em3>3.0

    authors: Barale R,Marrazzini A,Bacci E,Di Sibio A,Tessa A,Cocchi L,Scarcelli V,Lubrano V,Vassalle C,Landi S

    更新日期:1998-01-01 00:00:00

  • Cytosine arabinoside enhancement of gamma irradiation induced mutations in human T-lymphocytes.

    abstract::The frequency of 6-thioguanine resistant (TGr) mutants induced in human G0 phase T-lymphocytes by 200 cGy of gamma irradiation is greatly enhanced by incubation with cytosine arabinoside (ara-C) after irradiation. The mutant frequency increased with increasing incubation time in ara-C for up to 2 hr. This mutation ind...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.2850170403

    authors: O'Neill JP,Sullivan LM,Hunter TC,Nicklas JA

    更新日期:1991-01-01 00:00:00

  • Tandem-base mutations occur in mouse liver and adipose tissue preferentially as G:C to T:A transversions and accumulate with age.

    abstract::Tandem-base mutations (TBM) are associated with ultraviolet light and other mutagens. Herein, we report an age- and tissue-specific difference in the frequency of spontaneous TBM in Big Blue transgenic mice. A total of 390 mutants from liver and adipose tissue contained 17 and 4 TBM, respectively, while no TBM were de...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2280(1999)33:4<320::aid-em9>3.0

    authors: Buettner VL,Hill KA,Halangoda A,Sommer SS

    更新日期:1999-01-01 00:00:00

  • Association of cytochrome P450 1B1 haplotypes with head and neck cancer risk.

    abstract::Genetic polymorphisms have been reported in several cytochrome P450 (CYP) genes, including CYP1B1 which metabolically activates procarcinogens present in tobacco to carcinogenic intermediates. This study used a case-control approach in North Indian population to determine associations between genetic variants in CYP1B...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.22098

    authors: Katiyar T,Maurya SS,Hasan F,Singh AP,Khan AJ,Hadi R,Singh S,Bhatt MLB,Parmar D

    更新日期:2017-07-01 00:00:00

  • Confirmation of the chromosome damaging effects of lamivudine in in vitro human peripheral blood lymphocytes.

    abstract::The aim of this study was to investigate genotoxic effects of lamivudine (an analogue of cytidine) using in vitro sister chromatid exchange (SCE), chromosome aberration (CA), and micronucleus (MN) tests in human peripheral lymphocytes. The cells were treated with 75, 100, 125, and 150 microg/ml concentrations of lamiv...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.20393

    authors: Bayram S,Topaktaş M

    更新日期:2008-05-01 00:00:00

  • Development of a high-throughput genotoxicity assay using Umu test strains expressing human cytochrome P450s and NADPH-P450 reductase and bacterial O-acetyltransferase.

    abstract::Umu test is one of the in vitro genotoxicity test that has been used widely. It was developed as a high-throughput test system using the 96-well microplate. We have previously constructed new umu test strains for the evaluation of genotoxicity of procarcinogenic metabolic products formed by cytochrome P450 (CYP) enzym...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.22092

    authors: Hori H,Hirata D,Fujii W,Oda Y

    更新日期:2017-05-01 00:00:00

  • Proposal of an in vivo comet assay using haemocytes of Drosophila melanogaster.

    abstract::This study presents the first application of an in vivo alkaline comet assay using haemocytes of Drosophila melanogaster larvae. These cells, which play a role similar to that of mammalian blood, can be easily obtained and represent an overall exposure of the treated larvae. To validate the assay, we evaluated the res...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.20604

    authors: Carmona ER,Guecheva TN,Creus A,Marcos R

    更新日期:2011-03-01 00:00:00

  • A functional SNP in the 3'-UTR of TAP2 gene interacts with microRNA hsa-miR-1270 to suppress the gene expression.

    abstract::The transporter associated with antigen processing 2 (TAP2) is involved in the development of multidrug resistance and the etiology of immunological diseases. In this study, we investigated whether the expression of TAP2 can be perturbed by single nucleotide polymorphisms (SNPs) located in 3'-untranslated region (3'-U...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.22159

    authors: Knox B,Wang Y,Rogers LJ,Xuan J,Yu D,Guan H,Chen J,Shi T,Ning B,Kadlubar SA

    更新日期:2018-03-01 00:00:00

  • Persistence of micronuclei in peripheral blood normochromatic erythrocytes of subchronically benzene-treated male mice.

    abstract::The kinetics of micronucleus (MN) induction and decline in blood normochromatic erythrocytes (NCE) of mice subchronically exposed to benzene was investigated during and after exposure. Swiss (ICR) male mice (10/group) were given 0.0, 36.6, 73.2, and 146.4 mg/kg body weight benzene by gavage daily for 14 days, except f...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.2860120306

    authors: Rithidech K,Au WW,Ramanujam VM,Whorton EB Jr,Legator MS

    更新日期:1988-01-01 00:00:00

  • Effect of sulforaphane on micronucleus induction in cultured human lymphocytes by four different mutagens.

    abstract::Isothiocyanates (ITCs) are commonly found in cruciferous vegetables. A variety of biological activities have been ascribed to ITCs, such as inhibition of cytochrome P450 enzymes and induction of phase II enzymes in animal models. ITCs are also able to block cell-cycle progression and induce apoptosis in human cancer c...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.20156

    authors: Fimognari C,Berti F,Cantelli-Forti G,Hrelia P

    更新日期:2005-12-01 00:00:00

  • Tissue-specific and time-dependent clonal expansion of ENU-induced mutant cells in gpt delta mice.

    abstract::DNA mutations play a crucial role in the origins of cancer, and the clonal expansion of mutant cells is one of the fundamental steps in multistage carcinogenesis. In this study, we correlated tumor incidence in B6C3F1 mice during the period after exposure to N-ethyl-N-nitrosourea (ENU) with the persistence of ENU-indu...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.22132

    authors: Nakayama T,Sawai T,Masuda I,Kaneko S,Yamauchi K,Blyth BJ,Shimada Y,Tachibana A,Kakinuma S

    更新日期:2017-10-01 00:00:00

  • Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: interlaboratory reproducibility and assessment.

    abstract::The L5178Y mouse lymphoma cell mutagenesis assay is used to detect the mutagenic activity of chemicals in a mammalian cell system. To evaluate this assay we compared the results of assays performed independently on 63 chemicals by laboratories at SRI International and Litton Bionetics, Inc. The two laboratories used s...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章

    doi:10.1002/em.2860120506

    authors: Caspary WJ,Daston DS,Myhr BC,Mitchell AD,Rudd CJ,Lee PS

    更新日期:1988-01-01 00:00:00