High metabolic potential may contribute to the success of ST131 uropathogenic Escherichia coli.

abstract：:An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conj...

journal_title：Journal of clinical microbiology

authors： Dittmar D,Cleary TJ,Castro A

更新日期：1979-04-01 00:00:00

abstract：:The detection of class A serine-carbapenemases among species of Enterobacteriaceae remains a challenging issue. Methods of identification for routine use in clinical microbiology laboratories have not been standardized to date. We developed a novel screening methodology suitable for countries with high basal levels of...

journal_title：Journal of clinical microbiology

authors： Pasteran F,Mendez T,Guerriero L,Rapoport M,Corso A

更新日期：2009-06-01 00:00:00

abstract：:Given the rise in the incidence of invasive fungal infections (IFIs) and the expanding spectrum of fungal pathogens, early and accurate identification of the causative pathogen is essential. We developed a panfungal PCR assay that targets the internal transcribed spacer 1 (ITS1) region of the ribosomal DNA gene cluste...

journal_title：Journal of clinical microbiology

authors： Lau A,Chen S,Sorrell T,Carter D,Malik R,Martin P,Halliday C

更新日期：2007-02-01 00:00:00

abstract：:Two in a group of five naturally seropositive donkey stallions were found to shed equine arteritis virus (EAV) in their semen as demonstrated by virus isolation. Direct intramuscular inoculation of sonicated semen from one virus-shedding stallion (S3) caused clinical disease in two donkeys from which virus was recover...

journal_title：Journal of clinical microbiology

authors： Paweska JT,Volkmann DH,Barnard BJ,Chirnside ED

更新日期：1995-12-01 00:00:00

abstract：:Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR. In this study, we attempt...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Tamura M,Asami Y,Kitamura E,Saito K,Suzuki T,Takahashi SN,Matsumoto K,Sawada S,Yokoyama E,Takasu T

更新日期：2008-05-01 00:00:00

abstract：:Charcoal-horse blood agar with 40 micrograms of cephalexin per ml, charcoal-horse blood agar with 3 micrograms of lincomycin per ml, charcoal agar with 3 micrograms of lincomycin per ml, and Legionella (buffered charcoal-yeast extract) agar with 3 micrograms of lincomycin per ml were compared for isolation of Bordetel...

journal_title：Journal of clinical microbiology

authors： Hoppe JE,Schwaderer J

更新日期：1989-05-01 00:00:00

abstract：:Colistin-nalidixic acid agar, although recently recommended as a replacement for blood agar for primary plating of urine specimens ( Fung et al., J. Clin. Microbiol. 16:632-636, 1982), has also been reported to suppress the growth of some strains of staphylococci that are susceptible to colistin (polymyxin E). The sus...

journal_title：Journal of clinical microbiology

authors： Fung JC,McKinley G,Tyburski MB,Berman M,Goldstein J

更新日期：1984-05-01 00:00:00

abstract：:Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), a...

journal_title：Journal of clinical microbiology

authors： Imaz MS,Comini MA,Zerbini E,Sequeira MD,Latini O,Claus JD,Singh M

更新日期：2004-02-01 00:00:00

abstract：:Macrorestriction profile analysis by pulsed-field gel electrophoresis (PFGE) was used to distinguish between seven isolates of Cowdria ruminantium from geographically different areas. Characteristic profiles were generated for each isolate by using the restriction endonucleases KspI, SalI, and SmaI with chromosomal si...

journal_title：Journal of clinical microbiology

authors： de Villiers EP,Brayton KA,Zweygarth E,Allsopp BA

更新日期：2000-05-01 00:00:00

abstract：:Direct tube coagulase testing for identification of Staphylococcus aureus from BACTEC culture broth showed a sensitivity, a specificity, and positive and negative predicative values of 34%, 100%, 100%, and 80.2% with 2 h of incubation and 65%, 98.7, 99.7%, and 88.6% with 4 h of incubation. Anaerobic blood culture cont...

journal_title：Journal of clinical microbiology

authors： Qian Q,Eichelberger K,Kirby JE

更新日期：2007-07-01 00:00:00

abstract：:Two extended-spectrum beta-lactamase (ESBL)-producing salmonella isolates, Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium, were analyzed. Both isolates produced the CTX-M-3 ESBL; however, their bla(CTX-M-3) genes were located on different plasmids. The serovar Typhimurium isolate a...

journal_title：Journal of clinical microbiology

authors： Baraniak A,Sadowy E,Hryniewicz W,Gniadkowski M

更新日期：2002-03-01 00:00:00

abstract：:Three new Salmonella serotypes belonging to Kauffmann's subgenus I (F. Kauffmann, The Bacteriology of Enterobacteriaceae, 1966) were identified. These serotypes were Salmonella brazos 6,14,18:a:e,n,z15, Salmonella midway 6,14,24:d:1,7, and Salmonella balboa 48a, 48b:z41:monophasic. ...

journal_title：Journal of clinical microbiology

authors： Sutch KE,Blackburn BO

更新日期：1980-06-01 00:00:00

abstract：:An outbreak of Clostridium perfringens food poisoning occurred among attendees of a firehouse luncheon. The predominant symptoms of diarrhea (100%) and abdominal pain (81%) among case-patients, the mean incubation period (13.4 h), and the mean duration of illness (21.2 h) were all characteristic of C. perfringens ente...

journal_title：Journal of clinical microbiology

authors： Gross TP,Kamara LB,Hatheway CL,Powers P,Libonati JP,Harmon SM,Israel E

更新日期：1989-04-01 00:00:00

abstract：:A cytochrome b (cytb) gene quantitative PCR (qPCR) assay was developed to diagnose malaria in travelers. First, manual and automated DNA extractions were compared and automated DNA extraction of 400 μl of blood was found to be more efficient. Sensitivity was estimated using the WHO international standard for Plasmodiu...

journal_title：Journal of clinical microbiology

authors： Farrugia C,Cabaret O,Botterel F,Bories C,Foulet F,Costa JM,Bretagne S

更新日期：2011-06-01 00:00:00

abstract：:The BD Phoenix system was evaluated for species-level identification of yeasts (250 clinical isolates) and compared with the Vitek 2 system, using ribosomal internal transcribed spacer (ITS) sequence analysis as the gold standard. Considering only the species included in each system's database, 96.3% (236/245) and 91....

journal_title：Journal of clinical microbiology

authors： Posteraro B,Ruggeri A,De Carolis E,Torelli R,Vella A,De Maio F,Ricciardi W,Posteraro P,Sanguinetti M

更新日期：2013-11-01 00:00:00

abstract：:Chronic infection with hepatitis B virus (HBV) is an important cause of cirrhosis and cancer of the liver. HBV is currently classified into eight genotypes, A to H. Accumulated evidence shows that the genotype influences both the clinical course of infection and the response to treatment. We describe a new method for ...

journal_title：Journal of clinical microbiology

authors： Malmström S,Berglin-Enquist I,Lindh M

更新日期：2010-04-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00

abstract：:A retrospective case record study was conducted that established a scoring tool based on clinical and iQ200 parameters, able to predict or rule out the clinical diagnosis of UTI in the majority of adult patients in an academic hospital. Automated standardized quantitative urine analysis, such as iQ200 analysis, is on ...

journal_title：Journal of clinical microbiology

authors： Foudraine DE,Bauer MP,Russcher A,Kusters E,Cobbaert CM,van der Beek MT,Stalenhoef JE

更新日期：2018-05-25 00:00:00

abstract：:Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect e...

journal_title：Journal of clinical microbiology

authors： Zanoni R,Krieg A,Peterhans E

更新日期：1989-03-01 00:00:00

abstract：:Diarrheal diseases are highly prevalent in Bangladesh. However, the relative contribution of diarrheagenic Escherichia coli organisms--those that are enterotoxigenic (ETEC), enteropathogenic (EPEC), enteroinvasive, enterohemorrhagic, enteroaggregative, and diffuse adherent--to diarrhea in Bangladeshi populations is no...

journal_title：Journal of clinical microbiology

authors： Albert MJ,Faruque SM,Faruque AS,Neogi PK,Ansaruzzaman M,Bhuiyan NA,Alam K,Akbar MS

更新日期：1995-04-01 00:00:00

abstract：:The beta and gamma genera of papillomaviruses consist of epidermodysplasia verruciformis-related human papillomaviruses (HPVs) and phylogenetically related cutaneous HPVs. Here, we have developed a consensus primer PCR assay and reverse line blot typing system coupled thereto (referred to as beta and gamma cutaneous H...

journal_title：Journal of clinical microbiology

authors： Brink AA,Lloveras B,Nindl I,Heideman DA,Kramer D,Pol R,Fuente MJ,Meijer CJ,Snijders PJ

更新日期：2005-11-01 00:00:00

abstract：:Thirty stool filtrates known to contain Clostridium difficile toxin based on previous testing on McCoy cells were tested for toxicity on primary African green monkey kidney (AGMK), McCoy, MRC-5, primary rhesus monkey kidney (RMK), and Vero cells. All 30 filtrates showed cytotoxic effect at greater than or equal to 1:1...

journal_title：Journal of clinical microbiology

authors： Maniar AC,Williams TW,Hammond GW

更新日期：1987-10-01 00:00:00

abstract：:We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory p...

journal_title：Journal of clinical microbiology

authors： Coenye T,Spilker T,Martin A,LiPuma JJ

更新日期：2002-09-01 00:00:00

abstract：:An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

journal_title：Journal of clinical microbiology

authors： Wallis C,Melnick JL,Longoria CJ

更新日期：1981-09-01 00:00:00

abstract：:Multidrug-resistant opportunistic pathogens have become endemic to the veterinary hospital environment. Escherichia coli isolates resistant to 12 antibiotics were isolated from two dogs that were housed in the intensive care unit at The University of Georgia Veterinary Teaching Hospital within 48 h of each other. Revi...

journal_title：Journal of clinical microbiology

authors： Sanchez S,McCrackin Stevenson MA,Hudson CR,Maier M,Buffington T,Dam Q,Maurer JJ

更新日期：2002-10-01 00:00:00

abstract：:Monoclonal antibody (MAb)-based sandwich direct enzyme-linked immunosorbent assay (MSD-ELISA) methods that can detect foot-and-mouth disease virus (FMDV) antigens, both multiserotype (MSD-ELISA/MS) (for O, A, C, and Asia 1) and single-serotype (MSD-ELISA/SS) (for O, A, and Asia 1, specifically), were developed. MAb 1H...

journal_title：Journal of clinical microbiology

authors： Morioka K,Fukai K,Yoshida K,Yamazoe R,Onozato H,Ohashi S,Tsuda T,Sakamoto K

更新日期：2009-11-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) has occasionally been associated with cases of interstitial pneumonitis, mainly in individuals with impaired cellular immunity. Here we report for the first time severe interstitial pneumonitis with simultaneous HHV-6 and Pneumocystis carinii infections in the lung tissue of a young patient...

journal_title：Journal of clinical microbiology

authors： Vuorinen T,Kotilainen P,Lautenschlager I,Kujari H,Krogerus L,Oksi J

更新日期：2004-11-01 00:00:00

abstract：:A VIM metallo-beta-lactamase-producing Aeromonas hydrophila strain carrying an integron-borne bla(VIM-4) gene was isolated from a cirrhotic patient's fecal sample in a Budapest hospital. The variable region of this integron is identical with that of a previously characterized integron from Pseudomonas aeruginosa clini...

journal_title：Journal of clinical microbiology

authors： Libisch B,Giske CG,Kovács B,Tóth TG,Füzi M

更新日期：2008-05-01 00:00:00

abstract：:We enrolled 22 patients with gas-forming pyogenic liver abscess in a study to assess the mechanism of gas formation. Klebsiella pneumoniae was cultured from specimens from all patients. Gas and pus samples from abscesses revealed four major components: nitrogen, oxygen, carbon dioxide, and hydrogen; this implicates mi...

journal_title：Journal of clinical microbiology

authors： Lee HL,Lee HC,Guo HR,Ko WC,Chen KW

更新日期：2004-06-01 00:00:00

abstract：:In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here...

journal_title：Journal of clinical microbiology

authors： Lodes MJ,Suciu D,Elliott M,Stover AG,Ross M,Caraballo M,Dix K,Crye J,Webby RJ,Lyon WJ,Danley DL,McShea A

更新日期：2006-04-01 00:00:00