Inflammatory and bone regulators expression in murine macrophages under exposure of commercial and experimental mineral trioxide aggregate.

Abstract:

BACKGROUND:Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non-surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF-α, IL-1β and IL-6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures. METHODS:Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental- and commercial-grey MTA co-cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA. RESULTS:The expression of TNF-α for both commercial and experimental MTA was higher, while the expression of IL-1β and IL-6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control. CONCLUSIONS:This study suggests that commercial and experimental MTA promotes anti-inflammatory processes, as well as bone healing capacity.

journal_name

Aust Dent J

authors

Pereda GO,Fudinaga AC,Beltrán HS,Peroni LA,Stach-Machado D

doi

10.1111/j.1834-7819.2012.01701.x

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

284-91

issue

3

eissn

0045-0421

issn

1834-7819

journal_volume

57

pub_type

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