Abstract：
:A major difficulty in photodynamic therapy is the poor solubility of the photosensitizer (PS) under physiological conditions which correlates with low bioavailability. PS aggregation leads to a decrease in the photodynamic efficiency and a more limited activity in vitro and in vivo. To improve the aqueous solubility and reduce the aggregation of 2,9(10),16(17),23(24)-tetrakis[(2-dimethylamino)ethylsulfanyl]phthal-ocyaninatozinc(II) (Pc9), the encapsulation into four poloxamine polymeric micelles (T304, T904, T1107 and T1307) displaying a broad spectrum of molecular weight and hydrophilic-lipophilic balance was investigated. The aqueous solubility of Pc9 was increased up to 30 times. Morphological evaluation showed the formation of Pc9-loaded spherical micelles in the nanosize range. UV/Vis and fluorescence studies indicated that Pc9 is less aggregated upon encapsulation in comparison with Pc9 in water-DMSO 2% and remained photostable. Pc9-loaded micelles generated singlet molecular oxygen in high yields. Photocytotoxicity assays using human nasopharynx KB carcinoma cells confirmed that the encapsulation of Pc9 in T1107 and T1307 increases its photocytotoxicity by 10 times in comparison with the free form in water-DMSO. In addition, Pc9 incorporated into cells was mainly localized in lysosomes.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
García Vior MC,Marino J,Roguin LP,Sosnik A,Awruch Jdoi
10.1111/j.1751-1097.2012.01229.xsubject
Has Abstractpub_date
2013-03-01 00:00:00pages
492-500issue
2eissn
0031-8655issn
1751-1097journal_volume
89pub_type
杂志文章abstract：:By a technique which combines rapid mixing of cells and hematoporphyrin (HP) with a short duration of illumination, the photodynamic inactivation of yeast cells was investigated, particularly, in seeking for the information of the location of HP at the time of action. The fluence-survival curves obtained under the con...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
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journal_title：Photochemistry and photobiology
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doi：10.1111/j.1751-1097.2012.01236.x
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb04194.x
更新日期：1990-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0678:acopia>2.0.co;2
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doi：10.1562/2006-09-09-RA-1036
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12470
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05092.x
更新日期：1994-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04120.x
更新日期：1989-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00367.x
更新日期：2008-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12361
更新日期：2015-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01052.x
更新日期：2012-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12430
更新日期：2015-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
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pub_type： 杂志文章
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.13221
更新日期：2020-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb01863.x
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb02227.x
更新日期：1992-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb08588.x
更新日期：1997-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb04229.x
更新日期：1992-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1992-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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更新日期：2005-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00285.x
更新日期：2008-09-01 00:00:00
abstract：:Solid-state NMR spectroscopy gives a powerful avenue for investigating G protein-coupled receptors and other integral membrane proteins in a native-like environment. This article reviews the use of solid-state (2)H NMR to study the retinal cofactor of rhodopsin in the dark state as well as the meta I and meta II photo...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2008.00510.x
更新日期：2009-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:The psaI and psaL genes were characterized from the cyanobacterium Synechococcus sp. strain PCC 7002. The gene organization was different from that reported for other cyanobacteria with psaI occurring upstream and being divergently transcribed from the psaL gene. Mutants lacking PsaI or PsaL were generated by interpos...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb02421.x
更新日期：1996-07-01 00:00:00
