Abstract:
OBJECTIVES:Streptococcus pneumoniae is one of the most common pathogens of otitis media (OM) that exists in biofilm, which enhances the resistance of bacteria against antibiotic killing and diagnosis, compared to the free-floating (planktonic) form. This study evaluated biofilm formation by S. pneumoniae on an abiotic surface and in the middle ear cavity in a rat model of OM. METHODS:In vitro biofilm formation was evaluated by inoculation of a 1:100 diluted S. pneumoniae cell suspension in a 96-well microplate. Adherent cells were quantified spectrophotometrically following staining with crystal violet by measurement of optical density at 570 nm. The ultrastructure of pneumococcal biofilm was assessed by scanning electron microscopy (SEM). For in vitro biofilm study, S. pneumoniae cell suspensions containing 1×10(7) colony forming units were injected through transtympanic membrane into the middle ear cavity of Sprague Dawley rats. The ultrastructure of middle ear mucus was observed by SEM 1 and 2 weeks post-inoculation. RESULTS:The in vitro study revealed robust biofilm formation by S. pneumoniae after 12-18 hours of incubation in high glucose medium, independent of exogenously supplied competence stimulating peptide and medium replacement. Adherent cells formed three-dimensional structures approximately 20-30 µm thick. The in vivo study revealed that ciliated epithelium was relatively resistant to biofilm formation and that biofilm formation occurred mainly on non-ciliated epithelium of the middle ear cavity. One week after inoculation, biofilm formation was high in 50% of the treated rats and low in 25% of the rats. After 2 weeks, biofilm formation was high and low in 25% and 37.5% of rats, respectively. CONCLUSION:The results imply that glucose level is important for the S. pneumoniae biofilm formation and S. pneumoniae biofilm formation may play important role in the pathophysiology of OM.
journal_name
Clin Exp Otorhinolaryngoljournal_title
Clinical and experimental otorhinolaryngologyauthors
Yadav MK,Chae SW,Song JJdoi
10.3342/ceo.2012.5.3.139subject
Has Abstractpub_date
2012-09-01 00:00:00pages
139-44issue
3eissn
1976-8710issn
2005-0720journal_volume
5pub_type
杂志文章abstract:OBJECTIVES:This experimental study investigated the possible protective effect of beta glucans on amikacin ototoxicity. METHODS:Thirty-eight rats with normal distortion product otoacoustic emissions (DPOAEs) were divided into four groups. Group K was the control group. Group A was injected intramuscularly (i.m.) with ...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2013.6.1.1
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVES:Free flaps are widely used to reconstruct head and neck defects. Despite the improvement in the surgical techniques and the surgeons' experiences, flap failures still occur due to thrombotic occlusion after small vessels anastomosis. To reduce the possibility of flap loss as a result of thrombotic occlusion,...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2015.01781
更新日期:2016-12-01 00:00:00
abstract::Meningioma is well known as common disease of the central nervous system, whereas primary extracranial meningioma is rare, representing 1% to 2% of all meningiomas. We have experienced a case of primary extracranial meningioma presenting as a right cheek mass. The tumor was completely excised via a right lateral rhino...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2013.6.4.266
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVES:Somatostatin inhibits lymph production and reduces lymph flow into the lymphatic duct. We hypothesized that octreotide, a long-acting somatostatin analog, would reduce drainage after neck dissection (ND) by reducing the overall lymphatic flow in the neck as well as thoracic duct flow. METHODS:From 2012 to 2...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2015.00073
更新日期:2016-06-01 00:00:00
abstract:Objectives:Fibro-osseous lesions of the paranasal sinuses can present various clinical conditions. This study aimed to report the long-term clinical course of benign fibro-osseous lesions (BFOLs) in the paranasal sinuses, including clinical and radiologic features. Methods:Radiologically confirmed BFOLs except osteoma...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2020.01522
更新日期:2020-11-04 00:00:00
abstract:OBJECTIVES:The purpose of this study was to evaluate the advantages and limitations of using a silicon tube to support the fractured orbital floor by a transantral approach. METHODS:A retrospective study was conducted from January 2000 to December. 2011 in 51 patients with pure orbital floor fractures. The patients un...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2015.8.3.250
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVES:To compare the velopharyngeal function, swallowing and speech of the conventional and modified radial forearm free flap (RFFF) for soft palate reconstruction. METHODS:Retrospective clinical study. Twenty-eight patients who underwent oropharyngeal reconstruction with RFFF were divided into two groups: 10 pat...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2008.1.3.161
更新日期:2008-09-01 00:00:00
abstract:OBJECTIVES:This study analyzed short-term prognosis in patients with acute low frequency hearing loss (ALHL), and also investigate hearing recovery rates in patients with ALHL accompanied vertigo. METHODS:Retrospective medical record review of the patients who received treatment for ALHL between June 2005 and June 201...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2017.00948
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:The primary aim of this study was to determine whether 18F-FDG-PET/CT (PET/CT) scans provide additional diagnostic information in addition to the direct laryngoscopic examination (L/E) and contrast-enhanced CT (CT) in patients with glottic cancer during the initial evaluation. METHODS:Fifty-five consecutive...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2008.1.1.35
更新日期:2008-03-01 00:00:00
abstract:OBJECTIVES:In onco-hematological diseases, the incidence of paranasal sinuses infection dramatically increase and requires a combination of medical and surgical therapy. Balloon dilatation surgery (DS) is a minimally invasive, tissue preserving procedure. The study evaluates the results of DS for rhinosinusitis in immu...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2014.7.4.302
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVES:Patients with bilateral vocal fold paralysis (BVFP) theoretically have difficulty producing voiceless consonants. However, perceptual studies have revealed clear production of voiceless consonants with good articulation scores in nontracheostomized patients. The purpose of this study was to clarify the produ...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2016.00626
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:To find the main cause of facial nerve dysfunction in vestibular schwannoma (VS) surgery and review the prognosis of facial function in relation to tumor size, preoperative facial function and surgical approach. METHODS:We reviewed the surgical outcome of 134 patients with VS treated in our department betwe...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2011.4.4.168
更新日期:2011-12-01 00:00:00
abstract::Cavernous hemangioma seems to most frequently arise in the posterior portion of the external auditory canal. However, they rarely occur in the tympanic membrane. A 49-year-old male patient was referred for evaluation of right-sided pulsatile tinnitus that he'd experienced for the previous 2 years. Temporal bone comput...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2011.4.2.109
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVES:The authors would report the results of definitive radiation therapy (RT) for early glottic cancer by two different radiation dose schedules. METHODS:From February of 1995 till June of 2008, 157 patients with T1-2N0 glottic cancer were treated with curative RT at Samsung Medical Center. All patients had squ...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2012.5.2.94
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVES:There have been many studies on the relationship between diabetes mellitus and presbycusis. Microangiopathy and neuropathy that's caused by chronic hyperglycemia may lead to damage to the inner ear. Several clinical studies on humans and animal studies have been performed to investigate the association betwe...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2008.1.4.211
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the effect of the presence of one or two arytenoids on early/late period swallowing-aspiration functions. METHODS:Supracricoid partial laryngectomy (SCPL) with the diagnosis of laryngeal cancer between 2012 and 2014 were retrospectively evaluated. The patients were categ...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2015.01837
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:The melanoma antigen gene (MAGE) and synovial sarcoma on X chromosome (SSX) gene families are silent in most normal adult tissues, but are expressed in a variety of malignant lesions. Therefore, detection of MAGE and SSX transcription may be useful for the diagnosis of head and neck cancers. The aim of this ...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2008.1.2.97
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVES:This study was conducted to investigate the current practices of Asian otolaryngologists for laryngopharyngeal reflux (LPR). METHODS:An online survey about LPR was sent to 2,000 members of Asian otolaryngological societies, and a subgroup analysis was performed between Western and Eastern Asian otolaryngolo...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2019.01669
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVES:The transoral removal of stones by sialodochoplasty has been popularized in the treatment of submandibular sialolithiasis. However, the effectiveness of sialodochoplasty is controversial, and there are no reports on the long-term outcomes of this procedure. The purpose of this study was to assess the effecti...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2012.5.1.34
更新日期:2012-03-01 00:00:00
abstract::Granulomatosis with polyangiitis (GPA) is an idiopathic vasculitis of medium and small arteries, characterized by necrotizing granulomatous inflammation. GPA typically affects upper and lower respiratory tract with coexisting glomerulonephritis. This disease is generally characterized by antineutrophil cytoplasm antib...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章,评审
doi:10.21053/ceo.2016.9.1.8
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVES:In an effort to improve hearing aid users' satisfaction, recent studies on trainable hearing aids have attempted to implement one or two environmental factors into training. However, it would be more beneficial to train the device based on the owner's personal preferences in a more expanded environmental aco...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2015.01690
更新日期:2017-03-01 00:00:00
abstract::Laser surgery (LS) or radiotherapy (RT) is normally recommended in early glottic cancer. The objective of this study was to perform a comprehensive meta-analysis of acoustic and perceptual outcomes to compare voice quality of LS or RT in early-stage glottic cancer. Data sources were obtained after searching PubMed, Go...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章,评审
doi:10.21053/ceo.2018.00990
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVES:To prevent hypoparathyroidism after thyroidectomy, preservation of the parathyroid glands and their vascularity are essential. The aim of this study was to determine the association between postoperative parathyroid function and the viability of the parathyroid glands during thyroidectomy. METHODS:We prospe...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2016.00724
更新日期:2017-09-01 00:00:00
abstract::A schwannoma of the larynx is a rare benign tumor that usually presents as a submucosal mass in the pyriform sinus and the aryepiglottic space, and this type of schwannoma constitutes a diagnostic and therapeutic challenge for otolaryngologists. We present here two cases of supraglottic schwannomas that were misdiagno...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2010.3.3.166
更新日期:2010-09-01 00:00:00
abstract:OBJECTIVES:We aimed to investigate the clinical results of atticoantrotomy in patients with an attic cholesteatoma. METHODS:Ninety-eight ears in 98 patients were operated on using atticoantrotomy between October 2002 and December 2006. A retrospective review of the otology database (operative findings and methods, pos...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2009.2.1.39
更新日期:2009-03-01 00:00:00
abstract::Juvenile hyaline fibromatosis (JHF) is a rare, progressive autosomal recessive disease that's characterized by papulonodular skin lesions, soft tissue masses, joint contractures, gingival hypertrophy and osteolytic bone lesions. We present here the case of a 2-yr-old boy with JHF along with a review of the relevant li...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2010.3.2.102
更新日期:2010-06-01 00:00:00
abstract::Prognosis in relapsed metastatic head and neck squamous cell cancer (RM-HNSCC) is dismal. Platinum based chemotherapy in combination with Cetuximab is used in first-line setting, while no further validated options are available at progression. Immunotherapy has produced durable clinical benefit in some patients with R...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2018.00150
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:Autologous costal cartilage is a promising alternative for mastoid obliteration. However, donor-site morbidities of the chest wall limit the use of this graft. To address this issue, we have developed a minimally-invasive technique of harvesting costal cartilage and report donor site morbidity associated wit...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2015.8.1.13
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVES:To compare voice parameters in subjects with different hearing level. METHODS:The evaluation consisted of Multi-Dimensional Voice Program (MDVP) and electroglottography. Group 1 consisted of normal hearing subjects which is bilateral average hearing better than 25 decibels (dB) whereas group 2 consisted of ...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.21053/ceo.2015.01900
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:The number of patients with bilateral cochlear implant (CI) has gradually increased as patients and/or parents recognize its effectiveness. The purpose of this report is to evaluate the efficacy of 29 bilateral CI out of 169 pediatric CI users, who received auditory-verbal/oral habilitation at our hearing ce...
journal_title:Clinical and experimental otorhinolaryngology
pub_type: 杂志文章
doi:10.3342/ceo.2012.5.S1.S24
更新日期:2012-04-01 00:00:00