Abstract:
:Osteoblasts are believed to differentiate into osteocytes, becoming embedded in bone, or to undergo apoptosis after the bone formation phase. The regulation of this terminal differentiation seems to be critical for bone homeostasis. However the mechanism remains unclear and there is no assay system currently available to analyze this process. To address this issue, we developed a new model in which osteoblasts are cultured on a type I collagen gel layer with osteogenic supplements β-glycerophosphate and ascorbic acid. Cellular behavior was analyzed by electron microscopy, immunohistochemistry and real-time RT-PCR. Osteoblasts gradually migrated into the gel, produced collagen fibrils, and differentiated to osteocytic cells with bone lacunae- and canaliculi-like mineralization. Osteocalcin, DMP-1 and SOST protein expression was mainly expressed in the migrated cells within the mid-layer of the gel. Osteoblastic (ALP and osteocalcin) and osteocytic (PHEX, DMP-1 and SOST) mRNA expression was significantly increased compared with those of the cells cultured on plastic dishes alone after 21 days. The number of TUNEL-positive apoptotic cells gradually increased, reaching a maximum at 28 days. The cells were distributed at the surface and in the mid-layer of the gel at 7 days and after 14 days of culture, respectively. These data indicate that our model reproduces transition from osteoblasts to osteocytes, suggesting the following: 1) migration of osteoblasts into collagen gel may play a critical role in osteocytic differentiation; and 2) spatiotemporal gene expression and apoptosis may be involved in the terminal differentiation of osteoblasts. Our model will make it possible to study the mechanism of transition from osteoblast to osteocyte, and both cell type-related diseases including osteoporosis and osteonecrosis.
journal_name
Bonejournal_title
Boneauthors
Uchihashi K,Aoki S,Matsunobu A,Toda Sdoi
10.1016/j.bone.2012.09.001subject
Has Abstractpub_date
2013-01-01 00:00:00pages
102-10issue
1eissn
8756-3282issn
1873-2763pii
S8756-3282(12)01225-2journal_volume
52pub_type
杂志文章相关文献
BONE文献大全abstract:PURPOSE:Clinical and in vitro studies suggest that subchondral bone sclerosis due to abnormal osteoblasts is involved in the progression of osteoarthritis (OA). Human osteoblasts isolated from sclerotic subchondral OA bone tissue show an altered phenotype, a decreased canonical Wnt/ß-catenin pathway, and a reduced mine...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.02.001
更新日期:2015-06-01 00:00:00
abstract:INTRODUCTION:Studies on the association of vitamin D and bone mineral density (BMD) in adolescence and young adults have shown contrasting results. None of these studies have examined the course and baseline in vitamin D intake. The purpose of this study was to examine the association between baseline and the course of...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.10.055
更新日期:2009-03-01 00:00:00
abstract::Odontoblasts and osteoblasts are two among the myriads of cell types present in the craniofacial complex. Both have a common ectomesenchymal origin and secrete macromolecules that are necessary for the formation of dentin and alveolar bone via matrix-mediated mechanisms. The mineralized matrices of bone and dentin dif...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.05.013
更新日期:2005-10-01 00:00:00
abstract:INTRODUCTION:Teriparatide is a bone formation agent that increases bone turnover and mass, resulting in an increase in bone strength and a decrease in fracture risk. METHODS:The primary purpose of this analysis was to evaluate the association between pretreatment bone turnover marker (BTM) concentrations and the absol...
journal_title:Bone
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.bone.2006.02.003
更新日期:2006-08-01 00:00:00
abstract::Collagen sponge disks (6 mm diameter, 1 mm thickness) were impregnated with recombinant human bone morphogenetic protein-2 (rhBMP-2) (5 microg/disk) and implanted onto the back muscles of mice. Ten or 20 mg/kg per day of Rolipram, a selective inhibitory agent to phosphodiesterase type 4 (PDE-4), or vehicle, was inject...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00681-6
更新日期:2002-04-01 00:00:00
abstract::Hypophosphatasia (HPP) is the inborn-error-of-metabolism characterized enzymatically by insufficient activity of the tissue-nonspecific isoenzyme of alkaline phosphatase (TNSALP) and caused by either mono- or bi-allelic loss-of-function mutation(s) of the gene ALPL that encodes this cell surface phosphomonoester phosp...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.02.025
更新日期:2019-05-01 00:00:00
abstract::The influence of TGF beta 1 on bone was studied in a titanium device implanted into the tibia of rabbits. TGF beta 1 was infused via an Alzet osmotic pump calibrated to deliver at a rated of 200ng daily for 2 weeks before replacement. A hollow channel is incorporated into the device into which tissue can grow, and the...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(95)00324-7
更新日期:1995-10-01 00:00:00
abstract::Within each sex, there is an association between hip fracture risk and the size of the proximal femur, with larger femurs apparently more susceptible to fracture. Here, we investigate whether the thickness and density of the femoral cortex play a role in this association: might larger femurs harbour focal, cortical de...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.06.024
更新日期:2015-12-01 00:00:00
abstract::The combination of PTH with OPG has been proposed as a potential therapy in patients with severe osteoporosis. In the present study, we examined the bone material of aged ovariectomized (OVX) rats treated either with PTH (1-34) or OPG alone or in combination of both. The micro- and nanostructural characteristics of th...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.03.013
更新日期:2005-07-01 00:00:00
abstract::The effects of prostaglandin E2 (PGE2) were histomorphometrically evaluated in cancellous bone of the axial skeleton of ovariectomized, osteopenic rats. Four months following bilateral ovariectomy (OVX) and sham-ovariectomy (SHAM) at 3 months of age, rats received daily subcutaneous injections of PGE2 at 0, 0.3, 1.0, ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(90)90057-6
更新日期:1990-01-01 00:00:00
abstract::On the basis of studies with bone-seeking isotopes, it is generally believed that the metabolic activity of osteoblasts of the trabecular bone is greater than that of the osteoblasts of the cortex. This implies that the oxidative activity, which provides the energy for biosyntheses, will also be greater in the former ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(89)90061-6
更新日期:1989-01-01 00:00:00
abstract::Alterations in bone strength and structure were found in knockout (KO) mouse strains with deletion of several acetylcholine receptors. Interestingly, the expression of the nicotinic acetylcholine receptors (nAChR) subunit α10 was down-regulated in osteogenic differentiated mesenchymal stem cells of patients with osteo...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.11.003
更新日期:2019-03-01 00:00:00
abstract::Hypophosphatasia (HPP) is a multi-systemic inborn disease with an extraordinary spectrum of severity, ranging from the absence of mineralization to high lethality and it involves different organs including bone, muscle, kidney, lung, gastrointestinal tract and the nervous system. The disease is characterized by low le...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.10.022
更新日期:2017-01-01 00:00:00
abstract::Spinal cord injury (SCI) results in a dramatic loss of bone mineral and a marked increase in fracture incidence in the femur; however, its effect on the femur's geometric structure and strength is poorly studied. The primary purpose of the present study was to assess the geometric structure, composition, and strength ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.10.012
更新日期:2005-02-01 00:00:00
abstract::The Sost gene encodes Sclerostin, an inhibitor of Wnt-signaling, generally considered a main response gene to mechanical loading in bone. Several papers describe that unloading leads to upregulation of Sost, which in turn may lead to loss of bone. These studies were based on whole bone homogenates or cortical bone. By...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.01.018
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVE:To evaluate the morphological changes that take place in the subchondral bone and calcified cartilage zone in the experimental anterior cruciate ligament (ACL) dog model of osteoarthritis (OA) and analyze concomitant changes in the level of MMP-13 and cathepsin K, as well as examine the therapeutic effects of...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2003.11.021
更新日期:2004-03-01 00:00:00
abstract::Interleukin-1 (IL-1) has been shown to promote osteoclast (OC) differentiation, in addition to acting as a survival factor for mature osteoclasts. In this study, we investigate the expression of IL-1 during human osteoclast formation, taking advantage of a recently reported in vitro culture system that generates human...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(99)00176-3
更新日期:1999-09-01 00:00:00
abstract::ED-71, a novel analog of 1alpha,25-(OH)2 D3, increases bone mass to a greater extent than alfacalcidol, an 1alpha,25-(OH)2 D3 prodrug. In this study, we used a murine bone marrow ablation model to compare the effect of ED-71 on bone formation and resorption in vivo with that of 1alpha,25-(OH)2 D3. We discovered that b...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.08.017
更新日期:2007-02-01 00:00:00
abstract::Depending on populations, 15 to 40% of patients have a familial form of Paget's disease of bone (PDB), which is transmitted in an autosomal-dominant mode of inheritance with incomplete penetrance. To date, only SQSTM1 gene mutations have been linked to the disease. Several single nucleotide polymorphisms (SNPs) have b...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.01.007
更新日期:2016-03-01 00:00:00
abstract::Diabetes is associated with a number of lower extremity orthopedic conditions and complications including fractures, Charcot neuroarthropathy, plantar ulcers, and infection. These complications are of significant clinical concern in terms of morbidity, mortality, and socioeconomic costs. A review of each condition is ...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2015.07.029
更新日期:2016-01-01 00:00:00
abstract::The incidence of hip fractures in Oslo has shown a secular increase during the past decades. The main aims of the present study were to report the current incidence of hip fractures in Oslo and to determine whether there is a seasonal variation in the occurrence of fractures. Using the electronic diagnosis registers a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00603-2
更新日期:2001-11-01 00:00:00
abstract::Vertebral fracture risk is a heritable complex trait. The aim of this study was to identify genetic susceptibility factors for osteoporotic vertebral fractures applying a genome-wide association study (GWAS) approach. The GWAS discovery was based on the Rotterdam Study, a population-based study of elderly Dutch indivi...
journal_title:Bone
pub_type: 杂志文章
doi:
更新日期:2014-02-01 00:00:00
abstract::Obesity in humans is associated with increased bone mass. Leptin, a hormone produced by fat cells, functions as a sentinel of energy balance, and may mediate the putative positive effects of body mass on bone. We performed studies in male C57Bl/6 wild type (WT) and leptin-deficient ob/ob mice to determine whether body...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.10.058
更新日期:2009-03-01 00:00:00
abstract::Large, cylindrical implants of a porous calcium phosphate ceramic ("hydroxyapatite" starting material, HAC) were used to replace far greater than critical-sized sections of the midshaft of sheep tibiae and retrieved at 2 and 9 months; external fixation was used in the first 5 months. Excellent clinical function of the...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(99)00083-6
更新日期:1999-06-01 00:00:00
abstract::The deformation mechanism of bone at different hierarchical levels has been of wide interest. The important features of bone, its anisotropy and orientation dependent deformation are equally important, which have also gained a long run discussion. Most of the studies are concentrated on protein-rich collagen fibres an...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.01.394
更新日期:2009-06-01 00:00:00
abstract::Bone age is used widely by pediatricians to assess the skeletal maturity of a child and predict growth potential. This entails measuring the size of secondary ossification centers (SOCs), which develop with age in the ends of long bones, which are initially cartilaginous. However, little is presently known about the d...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115701
更新日期:2021-01-01 00:00:00
abstract::The signals that allow bone to adapt to its mechanical environment most likely involve strain-mediated fluid flow through the canalicular channels. Fluid can only be moved through bone by cyclic loading, and the shear stresses generated on bone cells are proportional to the rate of loading. The proportional relation b...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/s8756-3282(02)00707-x
更新日期:2002-05-01 00:00:00
abstract::Recent increasing evidence supports a role for neuronal type signaling in bone. Specifically glutamate receptors have been found in cells responsible for bone remodeling, namely the osteoblasts and the osteoclasts. While most studies have focused on ionotropic glutamate receptors, the relevance of the metabotropic glu...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.10.025
更新日期:2017-01-01 00:00:00
abstract::The formation of cartilage prior to bone in an endochondral bone formation process suggests that some osteogenic factors exist in the cartilage. This osteogenic activity of cartilage or cartilage cells was examined by implanting ribs and costal cartilage zone into a subcutaneous pocket of abdominal wall, or the growth...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(92)90455-6
更新日期:1992-01-01 00:00:00
abstract::Alagille syndrome (ALGS) is an autosomal dominant disorder attributed to mutations in the Notch signaling pathway. Children with ALGS are at increased risk for fragility fracture of unknown etiology. Our objective was to characterize bone mass, geometry, and microarchitecture in children with ALGS. This was a cross-se...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115576
更新日期:2020-12-01 00:00:00