Abstract:
:Cryopreservation of islets of Langerhans offers a number of important benefits for attempts to cure diabetes by transplantation. In the published literature, a variety of cooling rates, ranging from 0.25 to 75 degrees C/min, in conjunction with warming rates of 4-200 degrees C/min have been proposed to give optimal preservation of islets. In view of the general importance of rates of temperature change in determining survival and because of the possibility of modulating tissue immunogenicity by freezing and thawing, we have studied the interaction of cooling rate and warming rate for isolated rat islets that had been either fully or partially equilibrated with 2 M dimethyl sulfoxide (DMSO). Batches of islets were stored at -196 degrees C after cooling at 0.3, 3.0, 10, 30, 60, 150, or greater than 1000 degrees C/min and then warmed at either 10 or 50 degrees C/min. Survival was assessed by measuring the secretion of insulin during static incubation in alternating nonstimulatory and stimulatory media. Cooling rates extending over three orders of magnitude proved not to be a major determinant of survival when the islets were equilibrated with 2 M DMSO: greater than 50% survival was achieved at all cooling rates studied when the warming rate was at 50 degrees C/min. Peak survival (83%) was attained at a cooling rate of 0.3 degrees C/min, but only slightly lower recoveries were obtained at 60 and greater than 1000 degrees C/min. However, in islets only partially equilibrated with cryoprotectants, functional recovery was highly dependent on the cooling and warming rates, with peak survivals after slow cooling and rapid warming. Full permeation of the tissue with cryoprotectant offered maximal recovery of function.
journal_name
Diabetesjournal_title
Diabetesauthors
Taylor MJ,Benton MJdoi
10.2337/diab.36.1.59subject
Has Abstractpub_date
1987-01-01 00:00:00pages
59-65issue
1eissn
0012-1797issn
1939-327Xjournal_volume
36pub_type
杂志文章相关文献
DIABETES文献大全abstract::Isologous pancreatic islets were implanted into the portal vein of rats with streptozotocin-induced diabetes. At intervals of from one to 32 days after transplantation, the intrahepatic islet grafts were examined histologically and ultrastructurally, and their vascular supply was determined by later perfusion studies....
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.26.3.201
更新日期:1977-03-01 00:00:00
abstract::Genetic factors are important in conferring diabetic nephropathy (DN) risk. The insertion/deletion (I/D) polymorphism of the ACE gene has been described to be associated with DN risk and progression. The renal lesions underlying DN in type 2 diabetes are heterogeneous; only a subset of patients, characterized by a fas...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.51.1.251
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:Human islet amyloid polypeptide (hIAPP) aggregation plays a major role in the development of islet amyloidosis in type 2 diabetes. It is known that extracellular hIAPP oligomers are toxic to pancreatic beta-cells and associated with apoptosis. We therefore investigated the molecular mechanism by which extrace...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db07-0178
更新日期:2007-09-01 00:00:00
abstract::The inhibitory action of insulin and proinsulin on basal and glucagon-activated glycogenolysis was studied in cultured rat hepatocytes containing [14C]glycogen. Insulin or proinsulin given as sole hormones in the presence of 5 mM glucose decreased basal release of [14C]glucose from [14C]glycogen to 20%. Half-maximal e...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.36.5.551
更新日期:1987-05-01 00:00:00
abstract::Enhancement of arachidonic acid metabolism results in increased insulin secretion. To determine which pathways of arachidonic acid metabolism were involved in this stimulation, we studied the effects of various inhibitors of arachidonate metabolism on arginine-induced insulin and glucagon secretion in the isolated, pe...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.10.929
更新日期:1984-10-01 00:00:00
abstract::We investigated whether the effect of troglitazone on glucose disposal is associated with altered insulin signaling. Nondiabetic first-degree relatives of type 2 diabetic patients (age 30 +/- 2 years, BMI 30 +/- 1 kg/m(2); n = 20) were randomized in a double-blind manner to 3 months of troglitazone (200 mg/day) or pla...
journal_title:Diabetes
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.2337/diabetes.51.9.2691
更新日期:2002-09-01 00:00:00
abstract::Exosomes are important for intercellular communication, but the role of exosomes in the communication between adipose tissue (AT) and the liver remains unknown. The aim of this study is to determine the contribution of AT-derived exosomes in nonalcoholic fatty liver disease (NAFLD). Exosome components, liver fat conte...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db20-0146
更新日期:2021-02-01 00:00:00
abstract::C57BL/6 (B6) mice develop glucose intolerance with age, whereas C3H/He (C3H) mice do not. In this study, we examined whether this differential glucose homeostasis was associated with differences of proteolytic activation of pancreatic prohormones. Radioimmunoassays showed comparable levels of fasting plasma insulin be...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.55.02.06.db05-0733
更新日期:2006-02-01 00:00:00
abstract::Diabetes diagnostic therapy and research would strongly benefit from noninvasive accurate imaging of the functional β-cells in the pancreas. Here, we developed an analysis of functional β-cell mass (BCM) by measuring manganese (Mn(2+)) uptake kinetics into glucose-stimulated β-cells by T1-weighted in vivo Mn(2+)-media...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db14-0864
更新日期:2015-06-01 00:00:00
abstract::Hyperinsulinemia was produced in fetal rhesus monkeys for 21 days in the last third of gestation by subcutaneous pork insulin injected at 19 U a day. Plasma insulin concentrations in treated fetuses (N = 4) were 3525 microU/ml. There was no difference in paired pre- and post-treatment fetal plasma glucose concentratio...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.28.12.1064
更新日期:1979-12-01 00:00:00
abstract::On its own, glucose is a major factor for proliferation of pancreatic beta-cells and is also an essential prerequisite for IGF-I and growth hormone-induced growth of these cells. p8 was originally identified as an emergency gene product upregulated in pancreatic acinar cells in response to acute pancreatitis. p8 was f...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.2007.s82
更新日期:2004-02-01 00:00:00
abstract::Capillary endothelial cells are thought to limit the transport of insulin across the endothelium, resulting in attenuated insulin action at target sites. Whether endothelial insulin transport is altered in dysglycemic insulin-resistant states is not clear and was therefore investigated in the JCR:LA-cp corpulent male ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.5.803
更新日期:2000-05-01 00:00:00
abstract::Plasma glucagon response to glucose in diabetic subjects was observed before and after treatment. In normal subjects, plasma glucagon concentrations decreased substantially after an oral glucose load. In all diabetic patients before treatment, plasma glucagon was not suppressed and rather tended to rise paradoxically ...
journal_title:Diabetes
pub_type: 临床试验,杂志文章
doi:10.2337/diab.27.12.1145
更新日期:1978-12-01 00:00:00
abstract::Insulin-dependent diabetes (type 1 diabetes) in the NOD mouse is a T-cell-mediated autoimmune disease. However, B-cells may also play a critical role in disease pathogenesis, as genetically B-cell-deficient NOD mice (NOD.microMT) have been shown to be protected from type 1 diabetes and to display reduced responses to ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.4.763
更新日期:2001-04-01 00:00:00
abstract::The influence of insulin on the intracellular free sodium and potassium ion concentrations ([Na+]i, [K+]i) and resting membrane potential of rat epididymal adipocytes was examined to determine its potential for mediating insulin's action on other cellular processes. Direct intracellular measurements of [Na+]i, [K+]i, ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.29.12.1040
更新日期:1980-12-01 00:00:00
abstract::Bariatric surgery has proven to be the most effective treatment for controlling hyperglycemia in severely obese patients with diabetes. We show that fibroblast growth factor 19 (FGF19), a gut hormone, is rapidly induced by bariatric surgery in rodents and humans. Administration of FGF19 achieves diabetes remission ind...
journal_title:Diabetes
pub_type: 杂志文章,随机对照试验
doi:10.2337/db18-1305
更新日期:2019-06-01 00:00:00
abstract::It has been suggested that the hemodynamic derangements present in diabetic ketoacidosis are the results not only of profound volume depletion but also of the effects of increased production of vasodilating prostaglandins (PGs), principally PGI2, released by adipose tissue. In animal and in vitro models, prostaglandin...
journal_title:Diabetes
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.2337/diab.45.5.602
更新日期:1996-05-01 00:00:00
abstract::Although many studies using rodent islets and insulinoma cell lines have been performed to determine the role of insulin in the regulation of islet function, the autocrine effect of insulin on insulin gene expression is still controversial, and no consensus has yet been achieved. Because very little is known about the...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0532
更新日期:2006-10-01 00:00:00
abstract::Sterol regulatory element-binding protein (SREBP)-1 transcription factors play a central role in energy homeostasis by promoting glycolysis, lipogenesis, and adipogenesis. The sterol regulatory element-binding protein gene (SREBF)-1 is a good candidate gene for obesity and obesity-related metabolic traits such as type...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.8.2153
更新日期:2004-08-01 00:00:00
abstract::Carbohydrate-responsive element-binding protein (ChREBP) is a glucose-sensing transcription factor required for glucose-stimulated proliferation of pancreatic β-cells in rodents and humans. The full-length isoform (ChREBPα) has a low glucose inhibitory domain (LID) that restrains the transactivation domain when glucos...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db15-0239
更新日期:2015-12-01 00:00:00
abstract::The anionic charge on the surface of the erythrocyte and the erythrocyte membrane content of sialic acid and acid glycosaminoglycans (GAGs) were evaluated in insulin-dependent diabetic patients who had albumin excretion rates less than 300 mg/24 h. In these subjects a statistically significant reduction of erythrocyte...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.6.745
更新日期:1988-06-01 00:00:00
abstract::In type 1 diabetes, a lifelong autoimmune disease, T cells infiltrate the islets and the exocrine pancreas in high numbers. CD8+ T cells are the main cell type found in the insulitic lesion, and CD8+ T cells reactive against beta cell antigens have been detected in the periphery and in the pancreas of subjects with sh...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db20-0908
更新日期:2021-01-07 00:00:00
abstract::Fetal hyperinsulinemia causes fetal arterial hypoxia because fetal O2 use increases, whereas the supply of O2 to the fetus does not. To find which of the fetal tissues accounts for such an increase in fetal O2 use, we examined the effect of plasma hyperinsulinemia on O2, glucose, and lactate use by the hindquarters of...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.5.597
更新日期:1989-05-01 00:00:00
abstract::The hyperglycemic patient remains persistently at risk for infectious complications. Whether ascribable to diabetes mellitus, to the administration of glucocorticoids, or to the infusion of hyperalimentation fluids, hyperglycemia may impair several mechanisms of humoral host defense, including such varied neutrophil f...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diab.39.3.271
更新日期:1990-03-01 00:00:00
abstract::Data is accumulating indicating that impaired insulin action predisposes to increased vascular smooth muscle (VSM) tone, the hallmark of hypertension associated with diabetes. During the last several years, it has been established that VSM is an insulin-sensitive tissue like skeletal muscle and adipocytes. Investigato...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.45.3.s47
更新日期:1996-07-01 00:00:00
abstract::Extracellular ATP (eATP) activates T cells by engaging the P2X7R receptor. We identified two loss-of-function P2X7R mutations that are protective against type 1 diabetes (T1D) and thus hypothesized that eATP/P2X7R signaling may represent an early step in T1D onset. Specifically, we observed that in patients with newly...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db17-1227
更新日期:2018-10-01 00:00:00
abstract::Active maintenance of β-cell identity through fine-tuned regulation of key transcription factors ensures β-cell function. Tacrolimus, a widely used immunosuppressant, accelerates onset of diabetes after organ transplantation, but underlying molecular mechanisms are unclear. Here we show that tacrolimus induces loss of...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db19-0828
更新日期:2020-02-01 00:00:00
abstract::Vitamin E content and biosynthesis of 12-hydroxyeicosatetraenoic acid (12-HETE) have been measured in platelets from type I diabetic subjects and age- and sex-matched, nondiabetic control subjects. Platelets from diabetic subjects synthesized significantly greater quantities of 12-HETE than did platelets from control ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.34.6.526
更新日期:1985-06-01 00:00:00
abstract::In peripheral tissues, insulin signaling involves activation of the insulin receptor substrate (IRS)-phosphatidylinositol 3-kinase (PI3K) enzyme system. In the hypothalamus, insulin functions with leptin as an afferent adiposity signal important for the regulation of body fat stores and hepatic glucose metabolism. To ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.2.227
更新日期:2003-02-01 00:00:00
abstract::To investigate the effects of insulin on platelets in obesity and in non-insulin-dependent diabetes mellitus (NIDDM)--classic insulin-resistant states--we determined ADP-induced platelet aggregation and platelet cGMP (guanosine 3',5'-cyclic monophosphate) content in platelet-rich plasma obtained from nine obese subjec...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.44.11.1318
更新日期:1995-11-01 00:00:00