Abstract:
:Human periodontal ligament (hPDL) fibroblasts play a major role during periodontitis and orthodontic tooth movement, mediating periodontal inflammation, osteoclastogenesis, and collagen synthesis. The highly COX-2-selective NSAID etoricoxib has a favorable systemic side effect profile and high analgesic efficacy, particularly for orthodontic pain. In this in vitro study, we investigated possible side effects of two clinically relevant etoricoxib concentrations on the expression pattern of mechanically strained hPDL fibroblasts and associated osteoclastogenesis in a model of simulated orthodontic compressive strain occurring during orthodontic tooth movement. hPDL fibroblasts were incubated for 72 h under physiological conditions with etoricoxib at 0 μM, 3.29 μM, and 5.49 μM, corresponding to clinically normal and subtoxic dosages, with and without mechanical strain by compression (2 g/cm2) for the final 48 h, simulating conditions during orthodontic tooth movement in compressive areas of the periodontal ligament. We then determined gene and/or protein expression of COX-2, IL-6, PG-E2, RANK-L, OPG, ALPL, VEGF-A, P4HA1, COL1A2, and FN1 via RT-qPCR, ELISA, and Western blot analyses as well as apoptosis, necrosis, cell viability, and cytotoxicity via FACS, MTT, and LDH assays. In addition, hPDL fibroblast-mediated osteoclastogenesis was assessed by TRAP staining in coculture with RAW267.4 cells for another 72 h. Gene and protein expression of all evaluated factors was significantly induced by the mechanical compressive strain applied. Etoricoxib at 3.29 μM and 5.49 μM significantly inhibited PG-E2 synthesis, but not COX-2 and IL-6 gene expression nor RANK-L-/OPG-mediated osteoclastogenesis or angiogenesis (VEGF-A). Extracellular matrix remodeling (COL1A2, FN1) and bone anabolism (ALPL), by contrast, were significantly stimulated particularly at 5.49 μM. In general, no adverse etoricoxib effects on hPDL fibroblasts regarding apoptosis, necrosis, cell viability, or cytotoxicity were detected. Clinically dosed etoricoxib, that is, a highly selective COX-2 inhibition, did not have substantial effects on hPDL fibroblast-mediated periodontal inflammation, extracellular matrix remodeling, RANK-L/OPG expression, and osteoclastogenesis during simulated orthodontic compressive strain.
journal_name
Mediators Inflammjournal_title
Mediators of inflammationauthors
Kirschneck C,Küchler EC,Wolf M,Spanier G,Proff P,Schröder Adoi
10.1155/2019/2514956subject
Has Abstractpub_date
2019-03-10 00:00:00pages
2514956eissn
0962-9351issn
1466-1861journal_volume
2019pub_type
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journal_title:Mediators of inflammation
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更新日期:2015-01-01 00:00:00
abstract::BCG, the only registered vaccine against Mycobacterial Tuberculosis (TB) infection, has been questioned for its protective efficacy for decades. Although lots of efforts were made to improve the BCG antigenicity, few studies were devoted to understand the role of host factors in the variability of the BCG protection. ...
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journal_title:Mediators of inflammation
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abstract:Background:Multiple sclerosis (MS) is a chronic debilitating disorder characterized by persisting damage to the brain caused by autoreactive leukocytes. Leukocyte activation is regulated by cytokines, which are readily detected in MS serum and cerebrospinal fluid (CSF). Objective:Serum and CSF levels of forty-five cyt...
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journal_title:Mediators of inflammation
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doi:10.1155/2016/7678542
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journal_title:Mediators of inflammation
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更新日期:2012-01-01 00:00:00
abstract::In respiratory diseases, there is an increased expression of multiple inflammatory proteins in the respiratory tract, including cytokines, chemokines, and adhesion molecules. Chemokines have been shown to regulate inflammation and immune cell differentiation. Moreover, many of the known inflammatory target proteins, s...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2013/791231
更新日期:2013-01-01 00:00:00
abstract::Little is known about the cause and pathophysiology of middermal elastolysis (MDE). In this condition, variable inflammatory infiltrate may be present or not together with loss of elastic fibres in the middermis that spares both papillary and lower reticular dermis. MDE may be a consequence of abnormal extracellular m...
journal_title:Mediators of inflammation
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doi:10.1155/2017/9524594
更新日期:2017-01-01 00:00:00
abstract::This study was designated to evaluate the influence of behavioral depression on the airway leukocyte recruitment in allergic animals. To achieve this, total and differential cell counts in bronchoalveolar (BAL) fluid of ovalbumin (OVA)-sensitized and depressed rats was evaluated. Inescapable electric footshock, applie...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/09629350124118
更新日期:2001-08-01 00:00:00
abstract::The clinical outcome of autologous adipose stem cell (ASC) treatment of patients with multiple sclerosis (MS) was investigated following one year of observation. Methods. The clinical and MRI outcomes of 16 ASC-treated patients with RRMS and SPMS are reported after a one-year follow-up period. Results. At 18 months of...
journal_title:Mediators of inflammation
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doi:10.1155/2016/5302120
更新日期:2016-01-01 00:00:00
abstract::Cardiovascular (CV) disease is the most common cause of premature mortality in patients with rheumatoid arthritis (RA). It is the result of an accelerated atherosclerotic process. Both RA and atherosclerosis are complex polygenic diseases. Besides traditional CV risk factors and chronic inflammation, a number of studi...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2012/574817
更新日期:2012-01-01 00:00:00
abstract::Dysfunctions of the L-arginine (L-arg)/nitric-oxide (NO) pathway are suspected to be important for the pathogenesis of multiple organ dysfunction syndrome (MODS) in septic shock. Therefore plasma concentrations of L-arg and asymmetric dimethylarginine (ADMA) were measured in 60 patients with septic shock, 30 surgical ...
journal_title:Mediators of inflammation
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doi:10.1155/2012/210454
更新日期:2012-01-01 00:00:00
abstract::Crohn's disease (CD) is a multifactorial chronic inflammatory bowel disease of unknown cause. The aim of the present study was to explore if mRNA over-expression of SSTR5 and CCR7 found in CD patients could be correlated to respective protein expression. When compared to healthy donors, SSTR5 was over-expressed 417 +/...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2009/285812
更新日期:2009-01-01 00:00:00
abstract::Increased insulin resistance and inflammatory action are observed in pregnancy-induced hypertension (PIH), but similar insulin resistance is observed also in successful pregnancy. To estimate insulin resistance and inflammatory activity in normal pregnancy and PIH, serum concentrations of free fatty acids (FFA; correc...
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pub_type: 杂志文章
doi:10.1155/2012/432575
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND AND AIM:Immunization with live virus vaccines may cause an immunosuppression with lymphopaenia, impaired cytokine production and defective lymphocyte response to mitogenes. These abnormalities were described in subjects vaccinated against measles. This study was performed to analyse the host immune response ...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/09629350310001599639
更新日期:2003-08-01 00:00:00
abstract:OBJECTIVE:The proapoptotic protein, granzyme B (GZB), was identified as a contributor to the atherosclerotic plaque instability and recently as inflammatory activator. We studied the release kinetics of GZB and other markers of inflammation such as high sensitivity C reactive protein (hsCRP), interleukin 18 (IL-18), an...
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doi:10.1155/2013/343268
更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1155/2021/8874339
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更新日期:2012-01-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is the most common respiratory virus and is associated with pediatric pneumonia, causing bronchiolitis and significant mortality in infants and young children. MicroRNAs (miRNAs) are endogenous noncoding small RNAs that function in gene regulation and are associated with host immune r...
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更新日期:2017-01-01 00:00:00
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