Abstract:
:The object of this study was to investigate the effect of molybdenum on the development of mouse preimplantation embryos cultured in vitro. Zygotes were flushed from one outbred mouse strain (Kunming), and then were cultured in potassium simplex optimized medium (KSOM) containing 0, 5, 10, 20, 40, 80, 120, and 160 µg/ml of molybdenum for 5 days until the mid-blastocyst stage. The addition of ≤ 20 µg/ml molybdenum did not affect the blastocyst and birth rates. Molybdenum at doses of 40 µg/ml and higher significantly decreased the cleavage, blastocyst and birth rates, the average cell number, and significantly increased the proportion of degenerative blastocysts. At 120 µg/ml molybdenum inhibited the blastocysts development to birth. At 160 µg/ml molybdenum caused overall developmental arrest (up to 16-cells) of embryos and their massive degeneration. In conclusion, molybdenum negatively affected the development of embryos in a dose-dependent manner. With lower doses (≤ 20 µg/ml), mouse embryos were not apparently damaged. With very high doses (≥ 40 µg/ml), embryo quality significantly decreased. This assessment of the effect of molybdenum on the preimplantation embryo is an initial survey of toxicological risk.
journal_name
Syst Biol Reprod Medjournal_title
Systems biology in reproductive medicineauthors
Bi CM,Zhang YL,Liu FJ,Zhou TZ,Yang ZJ,Gao SY,Wang SD,Chen XL,Zhai XW,Ma XG,Jin LJ,Wang Sdoi
10.3109/19396368.2012.743198subject
Has Abstractpub_date
2013-04-01 00:00:00pages
69-73issue
2eissn
1939-6368issn
1939-6376journal_volume
59pub_type
杂志文章abstract::Preimplantation embryos may have an increased risk of having mismatches due to the rates of cell proliferation and DNA replication. Elimination of mismatches in human gametes and embryos has not been investigated. In this study we developed a sensitive functional assay to examine the repair or elimination of mismatche...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2016.1232447
更新日期:2016-12-01 00:00:00
abstract::In order to identify unreliable data in a dataset of motility parameters obtained from a pilot study acquired by a veterinarian with experience in boar semen handling, but without experience in the operation of a computer assisted sperm analysis (CASA) system, a multivariate graphical and statistical analysis was perf...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2013.766281
更新日期:2013-06-01 00:00:00
abstract::One of the major causes of defective sperm function is oxidative stress, which limits the fertilizing potential of these cells as the result of collateral damage to proteins and lipids in the sperm plasma membrane. On this point, a derangement of both generation and neutralization of reactive oxygen species (ROS) is a...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2020.1793032
更新日期:2020-10-01 00:00:00
abstract::FSH in infertile patients may be measured in the normal range and abnormal semen analysis findings may be observed in patients with normal FSH values. A recent study predicts that the sperm morphology and concentration may be impaired if the FSH value is above 4.5 IU/L. Therefore, this study aimed to define a clinical...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2020.1758834
更新日期:2020-10-01 00:00:00
abstract::In the human, canonical histones are largely replaced by protamine 1 (P1) and protamine 2 (P2) during late spermatogenesis. Recent studies have demonstrated that abnormal replacement of the histones is associated with severe male infertility and has profound implications for early embryogenesis. In this review the his...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章,评审
doi:10.3109/19396361003653311
更新日期:2010-06-01 00:00:00
abstract::Both microdrop and open methods are commonly used for in vitro fertilization (IVF) protocols for embryo culture as well as oocyte insemination. However, few comparative studies evaluating the microdrop or open method of insemination on the fertilization outcome and subsequent embryo development have been performed. A ...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章,随机对照试验
doi:10.3109/19396368.2013.872707
更新日期:2014-06-01 00:00:00
abstract::Short gamete co-incubation (SGCO) consists in decreasing the duration of contact between oocytes and sperm from the standard overnight insemination (SOI) toward 2 hours. However, the effectiveness of this technique to improve in vitro fertilization and embryo transfer (IVF-ET) outcomes remains controversial. Our study...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2017.1336581
更新日期:2017-10-01 00:00:00
abstract::There are variant rates of oocyte degeneration after intracytoplasmic sperm injection (ICSI) among different patients. Oocyte degeneration after ICSI may reflect the cohort of oocyte quality and subsequent embryo development capacity and clinical outcome. This retrospective study analyzed 255 cycles with at least one ...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2016.1272648
更新日期:2017-04-01 00:00:00
abstract::Phthalates are ubiquitous industrial chemicals that are reported to adversely affect human reproductive outcomes. Divergent effects on semen quality have been reported in a limited number of studies. To assess the possible contribution of regional differences in phthalate exposure to these results, we wished to determ...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396360802055921
更新日期:2008-05-01 00:00:00
abstract::Establishing specific biomarkers for the assessment of human male fertility status is an important goal to ensure the fitness of the male contribution so as to support the birth of a healthy child. Spermatozoa are considered an optimal surrogate tissue for the evaluation of spermatogenic function. Unlike the cells of ...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章,评审
doi:10.3109/19396368.2011.637152
更新日期:2012-02-01 00:00:00
abstract::Assessment of sperm DNA damage has been suggested as a negative predictor of fertility potential. Multiple pathological factors acting at both the intra-testicular and post-testicular levels may contribute to sperm DNA damage. The relative contribution of each of these factors in an individual with high DNA damage (>3...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396360902787944
更新日期:2009-03-01 00:00:00
abstract::Pulsatile secretion of hormones in the hypothalamic-pituitary-gonadal axis is critical for normal functioning of the reproductive system. Thus, appropriate characterization of pulsatile secretion is important for identifying the (patho)physiology of reproductive conditions. Existing analysis methods often fail to adeq...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2017.1411541
更新日期:2018-02-01 00:00:00
abstract::The aim of this study was to evaluate the predictive value of the anti-Mullerian hormone (AMH) level for early pregnancy loss and to compare the significance of AMH level to age as prognostic factors of pregnancy loss in subfertile women. The outcome of 848 subfertile patients confirmed with intrauterine pregnancies b...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2020.1806944
更新日期:2020-12-01 00:00:00
abstract::Sperm progressive motility has been reported to be one of the key factors influencing in vitro fertilization rates. However, recent studies have shown that sperm DNA fragmentation is a more robust predictor of assisted reproductive outcomes including reduced fertilization rates, embryo quality, and pregnancy rates. Th...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2011.553984
更新日期:2011-06-01 00:00:00
abstract::A very common conception about the function of the spermatozoon is that its unique role is to transmit the paternal genome to the next generation. Most of the sperm genome is known to be condensed in many species by protamines, which are small and extremely positively charged proteins (50-70% arginine) with the functi...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2018.1482383
更新日期:2018-12-01 00:00:00
abstract::To date we have yet to examine whether amino acid (AA) transport in human ovarian follicles is affected by competitive inhibition. In contrast, transplacental transfer of AAs in late-gestation sheep is characterized by reciprocal competition. This phenomenon has been described by algebraic equations of umbilical uptak...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2017.1341962
更新日期:2017-10-01 00:00:00
abstract::Spermatozoa contain a complex population of RNAs including messenger RNAs (mRNAs) and small RNAs such as microRNAs (miRNA). It has been reported that these RNAs can be used to understand the mechanisms by which toxicological exposure affects spermatogenesis. The aim of our study was to compare mRNA and miRNA profiles ...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2015.1022835
更新日期:2015-06-01 00:00:00
abstract::In many species, extended semen can be stored at low temperatures to slow bacterial growth. However, boar semen performs poorly at temperatures below 15 °C and this poses unique challenges, as it is not easy to maintain a constant 15-19 °C during shipment. Some extenders have been formulated with egg yolk for storage ...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2015.1040137
更新日期:2015-01-01 00:00:00
abstract::For patients with recurrent implantation failure in IVF, histologic or transcriptomic testing of the endometrium during the mid-secretory phase is often considered. Histological dating of endometrial biopsies (Noyes criteria) can determine if endometrial morphology is consistent with the period of receptivity. Alterna...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2020.1824032
更新日期:2020-12-01 00:00:00
abstract::Complete deletions in the AZF (a, b, and c) sub-regions of the Y-chromosome have been shown to contribute to unexplained male infertility. However, the role of partial AZFc deletions in male infertility remains to be verified. Three types of partial AZFc deletions have been identified. They are gr/gr, b1/b3, and b2/b3...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2012.748851
更新日期:2013-04-01 00:00:00
abstract::For natural fertilization to occur successfully, millions of spermatozoa must be deposited in the lower end of the female genital tract during mating. Considerably fewer spermatozoa are required for fertilization when spermatozoa are deposited in the upper region of the female tract. The extreme case of assisted ferti...
journal_title:Systems biology in reproductive medicine
pub_type:
doi:10.3109/19396368.2010.507860
更新日期:2011-02-01 00:00:00
abstract::Maternal obesity can cause complications for both women and their offspring for generations. Therefore, we intended to verify the repercussions of induction of transgenerational obesity on biochemical parameters, reproductive performance, and congenital anomaly frequency in Wistar rats. Female rats were used from succ...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2017.1410866
更新日期:2018-02-01 00:00:00
abstract::The ICSI procedure was invented to treat severe male infertility but is often used even if the sperm quality parameters are normal. This practice has recently been called into question, but there is still no clear answer especially in terms of sperm morphology, regarding when it is necessary to perform ICSI and when c...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2019.1666436
更新日期:2019-12-01 00:00:00
abstract::Genomic disorders are defined as diseases caused by rearrangements of the genome incited by a genomic architecture that conveys instability. Y-chromosome related dysfunctions such as male infertility are frequently associated with gross DNA rearrangements resulting from its peculiar genomic architecture. The Y-chromos...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章,评审
doi:10.3109/19396368.2010.527427
更新日期:2011-02-01 00:00:00
abstract::The vascular endothelial growth factor (VEGF), a major angiogenic factor, is known to play an important role in the development of endometriosis. The aim of this study was to investigate the association of three VEGF (-460 C/T, +405 G/C, and +936 C/T) polymorphisms with the risk of endometriosis in the Tunisian popula...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2015.1041622
更新日期:2015-01-01 00:00:00
abstract::Multiple prevention therapy has gained importance for the prevention and treatment of sexually transmitted diseases, especially HIV/AIDS. Antiretroviral drugs encapsulated in nanoparticles have been developed for efficient delivery of the drugs to the vaginal surface. Lactoferrin nanoparticles (LFNPs) encapsulating an...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.1080/19396368.2018.1519047
更新日期:2019-06-01 00:00:00
abstract::At least in mammals, retinoic acid is a pivotal factor in maintaining the functionality of the testis, in particular, for the progression of germ cells from mitosis to meiosis. Removal of dietary vitamin A or a targeted deletion of retinoic acid receptor alpha gene (Rara), the receptor for retinoic acid, in mice, led ...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396360902896844
更新日期:2009-08-01 00:00:00
abstract::Sexual dysfunction is one of the diabetic complications in males. The present study aimed to evaluate the antidiabetic effect of α-mangostin and its protective role in sexual dysfunction of streptozotocin (STZ) induced diabetic male rats. Male Wistar rats were divided as control, diabetic control, diabetic rats admini...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2013.820369
更新日期:2013-12-01 00:00:00
abstract::The GIRK2-containing inward-rectifying K(+) ion channels have been implicated in mammalian spermatogenesis. While the Girk2 null mice are fertile, the male weaver transgenic mice carrying a gain-of-function mutation in the Girk2 gene are infertile. To establish the exact period of spermatogenesis affected by this muta...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2011.631685
更新日期:2011-12-01 00:00:00
abstract::Although intracytoplasmic sperm injection (ICSI) is now a widely-used technique, it is still of interest to improve our knowledge as to which is the best spermatozoon to be selected for ICSI. Infertile men have increased risks of producing aneuploid spermatozoa. Using hyaluronic acid (HA)-binding sperm selection may r...
journal_title:Systems biology in reproductive medicine
pub_type: 杂志文章
doi:10.3109/19396368.2014.948102
更新日期:2014-12-01 00:00:00