Abstract:
:Amyotrophic lateral sclerosis (ALS) is a lethal disease characterized by motor neuron degeneration and associated with aggregation of nuclear RNA-binding proteins (RBPs), including FUS. How FUS aggregation and neurodegeneration are prevented in healthy motor neurons remain critically unanswered questions. Here, we use a combination of ALS patient autopsy tissue and induced pluripotent stem cell-derived neurons to study the effects of FUS mutations on RBP homeostasis. We show that FUS' tendency to aggregate is normally buffered by interacting RBPs, but this buffering is lost when FUS mislocalizes to the cytoplasm due to ALS mutations. The presence of aggregation-prone FUS in the cytoplasm causes imbalances in RBP homeostasis that exacerbate neurodegeneration. However, enhancing autophagy using small molecules reduces cytoplasmic FUS, restores RBP homeostasis and rescues motor function in vivo. We conclude that disruption of RBP homeostasis plays a critical role in FUS-ALS and can be treated by stimulating autophagy.
journal_name
Acta Neuropatholjournal_title
Acta neuropathologicaauthors
Marrone L,Drexler HCA,Wang J,Tripathi P,Distler T,Heisterkamp P,Anderson EN,Kour S,Moraiti A,Maharana S,Bhatnagar R,Belgard TG,Tripathy V,Kalmbach N,Hosseinzadeh Z,Crippa V,Abo-Rady M,Wegner F,Poletti A,Troost D,Adoi
10.1007/s00401-019-01998-xsubject
Has Abstractpub_date
2019-07-01 00:00:00pages
67-84issue
1eissn
0001-6322issn
1432-0533pii
10.1007/s00401-019-01998-xjournal_volume
138pub_type
杂志文章abstract::The neuropathological staging model of Alzheimer's disease proposed by Braak and Braak [Acta Neuropathol (1991) 82:259] requires that the evolution of neurofibrillary pathology follows a predictable pattern that can be ordered in a regular regional hierarchy. We have operationalized the neuropathological staging syste...
journal_title:Acta neuropathologica
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abstract::Autopsy findings of a patient, with sialidosis type I phenotype carrying V217M/G243R mutations in the lysosomal sialidase gene and biochemically defined isolated sialidase deficiency, who died of intractable lymphoma at the age of 32 years, are described. Perikaryal expansion of cytoplasm was evident, mostly in motor ...
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journal_title:Acta neuropathologica
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abstract::A 1-year-old male chinchilla with a 2-week history of conjunctivitis suffered subsequently from neurological signs comprising seizures, disorientation, recumbency and apathy. After 3 weeks of progressive central nervous disease the animal was killed in view of the poor prognosis. A non-suppurative meningitis and polio...
journal_title:Acta neuropathologica
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doi:10.1007/s00401-002-0597-6
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journal_title:Acta neuropathologica
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doi:10.1007/BF00691074
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journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/BF00690466
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journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/BF00690831
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journal_title:Acta neuropathologica
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doi:10.1007/BF00307700
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abstract::A quantitative technique involving serial sectioning and semiautomatic morphometric analysis was used to assess the severity of the reduction in size of the major brain structures in cerebral hemispheres of children congenitally infected with HIV-1. Cerebral hemispheres from 12 children (18-48 months of age) who died ...
journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/s004010050594
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journal_title:Acta neuropathologica
pub_type: 杂志文章,评审
doi:10.1007/s004010050604
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abstract::Fifteen cases of mitochondrial myopathy, three cases of hereditary motor and sensory neuropathy (HMSN) VI, and 280 cases of neuropathies of different etiologies were examined by electron microscopy for the presence of mitochondrial abnormalities in the sural nerve. Altered mitochondrial were found in most cases of mit...
journal_title:Acta neuropathologica
pub_type: 杂志文章,评审
doi:10.1007/BF00293381
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journal_title:Acta neuropathologica
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journal_title:Acta neuropathologica
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journal_title:Acta neuropathologica
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abstract::We have recently shown that glioma cell lines, as well as cells of human malignant gliomas in situ, synthesize tropoelastin. In addition, glioma cells degrade tropoelastin using metalloproteinase(s), and the resulting peptides, incapable of assembling in the extracellular fibers, interact with the 67-kDa cell surface ...
journal_title:Acta neuropathologica
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doi:10.1007/s004010051004
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abstract::Canine distemper virus (CDV) causes severe immunosuppression and neurological disease in dogs, associated with demyelination, and is a model for multiple sclerosis in man. In the early stage of the infection, demyelination is associated with viral replication in the white matter. In acute demyelinating lesions there i...
journal_title:Acta neuropathologica
pub_type: 杂志文章,评审
doi:10.1007/s00401-004-0958-4
更新日期:2005-01-01 00:00:00
abstract::Peroxidase-labeled lectins were used for detection of specific monosaccharide residues in amyloid plaques in brains of scrapie-infected mice. The lectins tested recognize the following residues: beta-D-galactosyl (Ricinus communis agglutinin 120, RCA-1), alpha-D-galactosyl and alpha-D-galactopyranoside (Bandeirea simp...
journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/BF00688295
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abstract::A histochemical study is reported of regional differences of the lipid storage in a case of Niemann-Pick disease (NPD) type C. Besides tissues known to be affected (reticulo-endothelium, hepatocytes, nervous system), storage was demonstrated in adrenal cortical spongiocytes, sweat glands, renal glomerular and tubular ...
journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/BF00688393
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abstract::Various parts of the central and peripheral nervous system of the mutant hamster with hind leg paralysis were studied in the electron microscope. A number of alterations involving the myelin sheath, the axon and certain neuronal perikarya were observed. Anterior horn cells were apparently well preserved. This conditio...
journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/BF00691701
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abstract::Neuropathological studies have revealed that the brains of HIV-1-infected AIDS patients show the typical encephalitis and, in addition, neuronal loss. More recently, this neuronal cell loss has been thought to take place via programmed cell death (apoptosis) which has been demonstrated by an in situ end labelling (ISE...
journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/s004010050409
更新日期:1996-01-01 00:00:00
abstract::Adult white rats were injected daily for 1 month with aluminum chloride and killed. Aluminum was identified, quantitated, and localized in the brains of these rats. The only detectable anomalies were crystals of aluminum and phosphorus in lysosomes and lipofuscin granules in the cytoplasm of neurons. ...
journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/BF00707114
更新日期:1980-01-01 00:00:00
abstract::Uniform neuropathological changes are described in eight cases of the progressive encephalopathy syndrome with edema, hypsarrhythmia and optic atrophy (PEHO syndrome). Two of the autopsied patients were sisters and two other cases were familial. Macroscopically, cerebral and pronounced cerebellar atrophy was seen, the...
journal_title:Acta neuropathologica
pub_type: 杂志文章
doi:10.1007/BF00227717
更新日期:1993-01-01 00:00:00