Abstract:
:The present study investigated the effect of the VEGF165 gene on adhesion, migration, and proliferation of endothelial progenitor cells (EPCs) derived from peripheral blood of rabbits. Peripheral blood mononuclear cells were isolated from rabbits by density gradient centrifugation with Ficoll-Plaque Plus. EPCs were characterized by immunofluorescence and immunostaining. A pcDNA 3.0-hVEGF165 expression vector was constructed and EPCs were transfected with the pcDNA 3.0-hVEGF165 gene. The EPCs derived from peripheral blood of rabbits were successfully transfected with pcDNA 3.0-hVEGF165. ELISA showed that the expression of VEGF165 increased significantly in the EPCs transfected with the hVEGF165 gene compared to control cells. Compared to control EPCs, EPCs transfected with the hVEGF165 gene had significantly enhanced adhesion, migration, and proliferative ability in vitro.
journal_name
Biosci Trendsjournal_title
Bioscience trendsauthors
Gu X,Miao X,Xu J,Cao Jdoi
10.5582/bst.2012.v6.5.270subject
Has Abstractpub_date
2012-10-01 00:00:00pages
270-5issue
5eissn
1881-7815issn
1881-7823pii
595journal_volume
6pub_type
杂志文章abstract::The aim of this study was to explore the value of contrast-enhanced ultrasound (CEUS) in differentiating small renal masses. A total of 102 small renal masses (≤ 3 cm) in 99 patients were examined using conventional ultrasound (CUS) and CEUS, and the findings were reviewed and evaluated in comparison to pathology. Sig...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2015.01080
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abstract::Laparoscopic liver resection (LLR) has garnered attention as a new form of liver surgery. In China, many hepatobiliary surgeons are now encouraging the examination and assessment of LLC in order to improve its outcomes, and several young hepatobiliary surgeons recently shared their clinical experiences and the results...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2018.01086
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2011.v5.6.264
更新日期:2011-12-01 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章,meta分析,收录出版,评审
doi:10.5582/bst.2015.01139
更新日期:2015-10-01 00:00:00
abstract::Cardiomyocyte nutrient deprivation is a common clinical event that mediates various cardiac ischemic processes and is associated with autophagy activation and cell survival or death. Luteolin-7-O-glucoside (LUTG) was one of the flavonoid glycosides isolated from Dracocephalum tanguticum. Previous research had showed t...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2017.01111
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journal_title:Bioscience trends
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journal_title:Bioscience trends
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2019.01191
更新日期:2019-11-13 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2018.01198
更新日期:2018-01-01 00:00:00
abstract::A research project involving sheets of retinal pigment epithelium constructed from iPS cells derived from patients with age-related maculopathy is one step closer to being approved for clinical trials by the Japanese Government. Now is the time to make therapies using iPS cells clinically available. ...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2013-06-01 00:00:00
abstract::On October 25, 2018, the National Health Commission of China issued the National Essential Medicines List (2018 edition) [NEML (2018)]. The NEML (2018) contains 685 drugs, which consist of 417 chemicals and biological products and 268 Chinese patent medicines. Compared to the 2012 version of the NEML, a total number o...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2018.01265
更新日期:2018-01-01 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2016.01009
更新日期:2016-05-23 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2020.03093
更新日期:2020-09-21 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2017.01307
更新日期:2017-01-01 00:00:00
abstract::The coronavirus disease 2019 (COVID-19) virus is spreading rapidly, and scientists are endeavoring to discover drugs for its efficacious treatment in China. Chloroquine phosphate, an old drug for treatment of malaria, is shown to have apparent efficacy and acceptable safety against COVID-19 associated pneumonia in mul...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2020.01047
更新日期:2020-03-16 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章,评审
doi:
更新日期:2013-02-01 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2011.v5.2.69
更新日期:2011-01-01 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2012.v6.1.26
更新日期:2012-02-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Previous studies demonstrated that miR-15a-5p was probably associated with human hepatocellular carcinoma, while the function of miR-15a-5p in OA (Osteoarthritis) still remains unknown. Here, we uncovered the potential role of miR-15a-5p on OA pathogenesis and confirmed its predicted target VEGFA (Vascular Endothelial...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2016.01187
更新日期:2017-01-16 00:00:00
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journal_title:Bioscience trends
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doi:
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2010-10-01 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2017.01070
更新日期:2017-09-12 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::Granzyme 3 (Gr3) is known as a tryptase-type member of the granzyme family and exists in the granules of immunocompetent cells. Granule proteases including granzymes, are transported into the cytoplasm of tumor cells or virus-infected cells by perforin function, degrade cytoplasmic or nuclear proteins and subsequently...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.8.126
更新日期:2014-04-01 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2017.01110
更新日期:2017-07-24 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
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更新日期:2020-09-21 00:00:00
abstract::Human serum albumin (HSA) is used as an important plasma volume expander in clinical practice. In the present study, HSA was N-terminally PEGylated and a PEGylated HAS (PEG-HSA) carrying one chain of PEG (20 kDa) per HSA molecule was obtained. The purity, secondary structure and hydrodynamic radius of the modified pro...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract::Atrazine (ATR), one of the most widely used herbicides worldwide, has caused a series of toxicological and environmental problems. This study sought to investigate the effects of ATR on the immune system of mice. Four-week-old female C57B l/6 mice were treated with 5, 25, and 125 mg/kg ATR for 28 days. On day 29, bloo...
journal_title:Bioscience trends
pub_type: 杂志文章
doi:
更新日期:2013-08-01 00:00:00
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journal_title:Bioscience trends
pub_type: 杂志文章
doi:10.5582/bst.2015.01114
更新日期:2015-12-01 00:00:00