Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India.

Abstract:

Introduction:Coagulase Negative Staphylococci, the most commonly isolated pathogen are becoming emerging threats to the community as well as to the nosocomial environment. The present study underscores the distribution of Staphylococcal cassette chromosome mec (SCCmec) types among Methicillin resistant Coagulase Negative Staphylococci from the environmental origin. Methods and Materials:Environmental and food sample (n = 460) from different location of northeastern region of India were collected for a period of one year and were phenotypically and genotypically screened using cefoxitin disc and PCR techniques for mecA and mecC gene detection. All the MR-CoNS isolates possessing mecA gene were subjected to 16srDNA sequencing for species identification. SCCmec typing was determined by evaluating using primer sets from type I to type V. Antibiotic susceptibility testing was performed for all the isolates. Statistical analysis with chi-square test using SPSS-21 statistical software. Results:Methicillin resistance shown by one hundred forty three isolates were carried out for molecular analysis, among them 53.84% serves as mecA carrier. Distribution of Staphylococcus haemolyticus was more frequent and was found that SCCmec types II and V were predominant among the study isolates. Linezolid was the drug of choice for the CoNS isolates. Statistical analysis showed an insignificant result for the tested antibiotics and SCCmec types. Conclusion:This study therefore interprets the relative importance of SCCmec types among MR-CoNS isolates.

journal_name

Indian J Med Microbiol

authors

Bhowmik D,Chakraborty P,Bhowmick R,Sarkar R,Chetri S,Bhattacharjee A

doi

10.4103/ijmm.IJMM_18_285

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

522-525

issue

4

eissn

0255-0857

issn

1998-3646

pii

IndianJMedMicrobiol_2018_36_4_522_254391

journal_volume

36

pub_type

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