Real-time PCR HPV genotyping in fine needle aspirations of metastatic head and neck squamous cell carcinoma: Exposing the limitations of conventional p16 immunostaining.

Abstract:

BACKGROUND:Given the propensity for HPV-positive head and neck squamous cell carcinoma (HPV-HNSCC) to metastasize to cervical lymph nodes, fine needle aspiration (FNA) plays an important diagnostic role in their initial detection. Indeed, there is now an unwavering commitment to HPV testing of FNAs even in the absence of clear methodologic guidelines and threshold criteria. A particular difficulty pertains to the interpretation of p16 staining. DESIGN:Data was collected for 210 patients with suspected regionally metastatic HNSCC that had undergone FNA as part of standard clinical care. Initial HPV screening was performed on cell blocks with real-time PCR using primers targeting L1 of high-risk HPV types. Additional genotyping was performed on HPV-positive cases. The results were compared to p16 staining and subsequent excisions when available. RESULTS:Of the 207 samples with sufficient DNA, 175 (85%) were HPV positive. HPV-16 was the most commonly detected genotype (90%). Of the HPV-positive cases, the primary site was the oropharynx (n = 154, 88.0%), supraglottic larynx (n = 2, 1.1%), nasal cavity (n = 1, 0.6%), hypopharynx (n = 1, 0.6%) or unknown (n = 17, 9.7%). On comparison with 31 paired surgical excisions, HPV status was concordant in all cases (100% correlation). Of 142 HPV-positive cases with matching p16 stains, p16 staining was reported as positive (n = 85, 60%), focal (n = 27, 19%), negative (n = 24, 17%) or non-contributory (n = 6, 4%); and only 33% reached the standard threshold limit (i.e. 70%) for HPV positivity. CONCLUSION:For patients with metastatic HNSCC, real-time PCR of FNAs reliably reflects HPV status, and is superior to conventional p16 immunostaining.

journal_name

Oral Oncol

journal_title

Oral oncology

authors

El-Salem F,Mansour M,Gitman M,Miles BA,Posner MR,Bakst RL,Genden EM,Westra WH

doi

10.1016/j.oraloncology.2019.02.006

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

74-79

eissn

1368-8375

issn

1879-0593

pii

S1368-8375(19)30042-9

journal_volume

90

pub_type

杂志文章
  • Lung metastasectomy in adenoid cystic carcinoma (ACC) of salivary gland.

    abstract::To define the role of surgical management of lung metastases in ACC. Twenty ACC patients referred to lung metastasectomy were retrospectively reviewed. Twenty-six operations were performed; at the first metastasectomy, a resection with clear margins (R0) was achieved in 11 patients (55%), 3 are alive and well. Four ou...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2005.04.014

    authors: Locati LD,Guzzo M,Bossi P,Massone PP,Conti B,Fumagalli E,Bareggi C,Cantù G,Licitra L

    更新日期:2005-10-01 00:00:00

  • Histologic pattern of invasion and epithelial-mesenchymal phenotype predict prognosis in squamous carcinoma of the head and neck.

    abstract:INTRODUCTION:Disruption of E-cadherin function and increased expression of vimentin and the transcriptional oncogene, SOX2, are thought to characterize epithelial to mesenchymal transition (EMT) in HNSCC that contributes to invasive and metastatic behavior. To determine if such changes relate to prognosis or host immun...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2018.10.010

    authors: Wolf GT,Winter W,Bellile E,Nguyen A,Donnelly CR,McHugh JB,Thomas D,Amlani L,Rozek L,Lei YL,Head and Neck SPORE Program.

    更新日期:2018-12-01 00:00:00

  • Tumor cell proliferation and microsatellite alterations in human ameloblastoma.

    abstract::Ameloblastoma is the most common odontogenic tumor. It can exhibit a variety of histological patterns, a great infiltrative potential and a high recurrence rate. Mutations in microsatellite sequences are a hallmark of neoplastic transformation but little is known about their role in ameloblastoma development. In this ...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2006.12.004

    authors: Migaldi M,Sartori G,Rossi G,Cittadini A,Sgambato A

    更新日期:2008-01-01 00:00:00

  • Validation and characterisation of prognostically significant PD-L1+ immune cells in HPV+ oropharyngeal squamous cell carcinoma.

    abstract::We previously showed in human papillomavirus positive oropharyngeal squamous cell carcinoma (HPV+OPSCC) that the presence of intratumoral (IT) PD-L1+ immune cells (ICs) or CD8+ infiltrating ICs are of prognostic value. Here we report the prognostic significance of these immune biomarkers in an independent validation c...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2019.104516

    authors: Young RJ,Bressel M,Porceddu S,Cernelc J,Savas P,Liu H,Urban D,Thai AA,Cooper C,Fua T,Neeson P,Rischin D,Solomon B

    更新日期:2020-02-01 00:00:00

  • Tubulopapillary hidradenoma-like tumor of the mandible: Two case reports of an underrecognized entity.

    abstract::Tubulopapillary hidradenoma-like tumor of the mandible is extremely rare, with only 3 cases published in the English-language literature. The clinicopathological characteristics and histogenesis of this tumor are unclear. Few pathologists and perhaps fewer clinicians are familiar with this entity, and it is likely und...

    journal_title:Oral oncology

    pub_type: 信件

    doi:10.1016/j.oraloncology.2019.104448

    authors: Zeng M,Guo X,Chen X,Yang S

    更新日期:2020-01-01 00:00:00

  • Use of artificial intelligence in diagnosis of head and neck precancerous and cancerous lesions: A systematic review.

    abstract::This systematic review analyses and describes the application and diagnostic accuracy of Artificial Intelligence (AI) methods used for detection and grading of potentially malignant (pre-cancerous) and cancerous head and neck lesions using whole slide images (WSI) of human tissue slides. Electronic databases MEDLINE v...

    journal_title:Oral oncology

    pub_type: 杂志文章,评审

    doi:10.1016/j.oraloncology.2020.104885

    authors: Mahmood H,Shaban M,Indave BI,Santos-Silva AR,Rajpoot N,Khurram SA

    更新日期:2020-11-01 00:00:00

  • Unilateral endoscopic resection with transnasal craniectomy for sinonasal intestinal-type adenocarcinoma: A bi-institutional case-control study on 54 patients.

    abstract:OBJECTIVES:Intestinal-type adenocarcinoma (ITAC) of the ethmoid mostly affects patients exposed to wood dust and has been traditionally treated with bilateral resection. The aim of the present study was to validate unilateral endoscopic resection with transnasal craniectomy (uERTC) in selected ITACs. MATERIALS AND MET...

    journal_title:Oral oncology

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.oraloncology.2018.10.027

    authors: Schreiber A,Ferrari M,Mattavelli D,Rampinelli V,Taboni S,Vural A,Bignami M,Castelnuovo P,Nicolai P

    更新日期:2018-12-01 00:00:00

  • Nasopharyngeal cancer WHO type II-III: monoinstitutional retrospective analysis with standard and accelerated hyperfractionated radiation therapy.

    abstract::The aim of this study is to assess the impact of prognostic factors in patients with locoregionally advanced nasopharyngeal cancer (NPC), WHO type II-III, treated with two different radiation therapy (RT) schedules: standard radiation therapy (SRT), and accelerated hyperfractionated radiation therapy (HART), with or w...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/s1368-8375(01)00034-3

    authors: Franchin G,Vaccher E,Talamini R,Gobitti C,Minatel E,Politi D,Sartor G,Trovò MG,Barzan L

    更新日期:2002-02-01 00:00:00

  • Comparative study of sentinel lymph node biopsy in clinically N0 oral tongue squamous cell carcinoma: Long-term oncologic outcomes between validation and application phases.

    abstract:OBJECTIVE:This study tested the long-term outcomes of sentinel lymph node biopsy (SLNB) for oral tongue squamous cell carcinoma (SCC) during the transition from validation to application phase. MATERIALS AND METHODS:Sensitivity, negative predictive value (NPV), neck control rate, disease-specific survival (DSS), disea...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2015.07.007

    authors: Chung MK,Lee GJ,Choi N,Cho JK,Jeong HS,Baek CH

    更新日期:2015-10-01 00:00:00

  • Numerical aberrations of chromosomes 8, 9, 11, and 17 in squamous cell carcinoma of the pharynx and larynx: a fluorescence in situ hybridization and DNA flow cytometric analysis of 50 cases.

    abstract::Squamous cell carcinoma of the pharynx and larynx (SCCPL) is a genetically complex disease and is frequently associated with nonrandom chromosomal alterations. Fifty primary SCC of the pharynx (oropharynx, n=11): see and hypopharynx, n=11) and larynx ( n=28) were examined for numerical aberrations of chromosomes 8, 9,...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2003.09.008

    authors: Hardisson D,Alvarez-Marcos C,Salas-Bustamante A,Alonso-Guervós M,Sastre N,Sampedro A

    更新日期:2004-04-01 00:00:00

  • Prognostic value of log odds of positive lymph nodes in patients with resectable oral squamous cell carcinoma.

    abstract:OBJECTIVES:Log odds of positive lymph nodes (LODDS) was reported to be significantly associated with prognosis in several malignant tumors. However, few are the studies on the correlation between LODDS and overall survival (OS) in patients with oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS:A retrospective...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2020.104709

    authors: Jin W,Zhu Z,Wu Y,Ding X,Wu H,Song X,Wu Y

    更新日期:2020-09-01 00:00:00

  • External validation of the AJCC Cancer Staging Manual, 8th edition, in an independent cohort of oral cancer patients.

    abstract:OBJECTIVES:To evaluate the new American Joint Committee on Cancer (AJCC) cancer staging manual (8th edition) in an independent cohort of patients surgically treated for oral squamous cell carcinoma in order to determine whether the upstaging of pT and pN classification was indicative of a worse prognosis. METHODS:A co...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2017.05.020

    authors: Matos LL,Dedivitis RA,Kulcsar MAV,de Mello ES,Alves VAF,Cernea CR

    更新日期:2017-08-01 00:00:00

  • Hypermethylated ZNF582 and PAX1 are effective biomarkers for detection of oral dysplasia and oral cancer.

    abstract:OBJECTIVES:This study investigated whether the methylation of ZNF582, PAX1, SOX1, NKX6.1, and PTPRR genes in oral scrapings could be used to detect oral dysplasia and oral cancer and to predict oral cancer recurrence. MATERIALS AND METHODS:Oral scrapings were collected from 65 normal oral mucosa subjects, 107 oral pre...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2016.09.007

    authors: Cheng SJ,Chang CF,Lee JJ,Chen HM,Wang HJ,Liou YL,Yen C,Chiang CP

    更新日期:2016-11-01 00:00:00

  • Effects of metformin on head and neck cancer: a systematic review.

    abstract::Conventional therapeutic approaches for head and neck squamous cell carcinoma (HNSCC) are associated with many adverse effects that reduce quality of life. Therefore, identification of new less cytotoxic treatments is highly important. Metformin, which is commonly used for type 2 diabetes, may reduce cancer risk. A fe...

    journal_title:Oral oncology

    pub_type: 杂志文章,评审

    doi:10.1016/j.oraloncology.2015.01.007

    authors: Rêgo DF,Pavan LM,Elias ST,De Luca Canto G,Guerra EN

    更新日期:2015-05-01 00:00:00

  • Depth of invasion, size and number of metastatic nodes predicts extracapsular spread in early oral cancers with occult metastases.

    abstract:OBJECTIVE:Presence of extracapsular spread (ECS) significantly decreases survival in oral cancer patients. Considering its prognostic impact, we have studied the incidence and factors predicting ECS in clinically node negative early oral cancers. MATERIALS AND METHODS:We performed a retrospective chart review of 354 t...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2018.04.015

    authors: Mair MD,Shetty R,Nair D,Mathur Y,Nair S,Deshmukh A,Thiagarajan S,Pantvaidya G,Lashkar S,Prabhash K,Chaukar D,Pai P,Cruz AD,Chaturvedi P

    更新日期:2018-06-01 00:00:00

  • Odontogenic carcinosarcoma: A systematic review.

    abstract::The aim of this study was to integrate the available data published on odontogenic carcinosarcoma into a comprehensive analysis of their features, treatment and recurrence. An electronic search with no publication date or language restriction was undertaken in March 2018 in the following databases: Medline Ovid, PubMe...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2018.08.017

    authors: Schuch LF,de Arruda JAA,Silva LVO,Abreu LG,Silva TA,Mesquita RA

    更新日期:2018-10-01 00:00:00

  • A network meta-analysis in comparing prophylactic treatments of radiotherapy-induced oral mucositis for patients with head and neck cancers receiving radiotherapy.

    abstract:OBJECTIVES:The objective of this network meta-analysis is to optimize the prophylactic treatment for radiotherapy-induced oral mucositis in patients with head and neck cancer (HNSCC) receiving postoperative or definitive radiotherapy with or without chemotherapy. MATERIALS AND METHODS:We searched electronic databases ...

    journal_title:Oral oncology

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.oraloncology.2017.11.001

    authors: Peng H,Chen BB,Chen L,Chen YP,Liu X,Tang LL,Mao YP,Li WF,Zhang Y,Lin AH,Sun Y,Ma J

    更新日期:2017-12-01 00:00:00

  • A novel scoring model to predict benefit of additional induction chemotherapy to concurrent chemoradiotherapy in stage II-IVa nasopharyngeal carcinoma.

    abstract:OBJECTIVES:Induction chemotherapy (IC) is gaining recognition for the treatment of nasopharyngeal carcinoma (NPC). We aimed to develop a model to predict benefit from additional IC to concurrent chemoradiotherapy (CCRT). MATERIALS AND METHODS:From an NPC-specific database, 7413 patients with stage II-IVa disease who r...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2018.10.007

    authors: Peng L,Chen YP,Xu C,Tang LL,Chen L,Lin AH,Liu X,Sun Y,Ma J

    更新日期:2018-11-01 00:00:00

  • Verruciform xanthoma of the oral mucosa. Report of four cases and a review of the literature.

    abstract::We present four new cases of verruciform xanthoma (VX) in the oral mucosa and review the literature. Clinical, histological, and immunohistochemical features of four new cases of VX were analysed together with cases found in a review of the literature. Expression of CD-68 was studied by immunohistochemistry. Only 162 ...

    journal_title:Oral oncology

    pub_type: 杂志文章,评审

    doi:10.1016/s1368-8375(00)00068-3

    authors: Oliveira PT,Jaeger RG,Cabral LA,Carvalho YR,Costa AL,Jaeger MM

    更新日期:2001-04-01 00:00:00

  • Gender disparities in head and neck cancer chemotherapy clinical trials participation and treatment.

    abstract:OBJECTIVES:To characterize the representation of women in clinical trials directing the National Comprehensive Cancer Network (NCCN) guidelines for chemotherapy use in head and neck squamous cell carcinoma (HNSCC), as well as the relationship between gender and chemotherapy administration in the definitive treatment of...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2019.05.009

    authors: Benchetrit L,Torabi SJ,Tate JP,Mehra S,Osborn HA,Young MR,Burtness B,Judson BL

    更新日期:2019-07-01 00:00:00

  • ERCC1 plays an important role in predicting survival outcomes and treatment response for patients with HNSCC: a meta-analysis.

    abstract:BACKGROUND:Excision repair crosscomplementing-1 (ERCC1) has been reported to play a prognostic role and may indicate the treatment response in patients with head and neck squamous carcinoma (HNSCC). Nevertheless, the strength of evidence of ERCC1 predicting these two clinical outcomes are still controversial. METHODS:...

    journal_title:Oral oncology

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.oraloncology.2015.02.094

    authors: Xuelei M,Jingwen H,Wei D,Hongyu Z,Jing Z,Changle S,Lei L

    更新日期:2015-05-01 00:00:00

  • Gene expression profiling by DNA microarrays and its application to dental research.

    abstract:UNLABELLED:DNA microarray technology has been used for genome-wide gene expression studies that incorporate molecular genetics and computer science skills on massive levels. The technology permits the simultaneous analysis of tens of thousands of genes for the purposes of gene discovery, disease diagnosis. improved dru...

    journal_title:Oral oncology

    pub_type: 杂志文章,评审

    doi:10.1016/s1368-8375(02)00013-1

    authors: Kuo WP,Whipple ME,Sonis ST,Ohno-Machado L,Jenssen TK

    更新日期:2002-10-01 00:00:00

  • Growth inhibition and apoptosis by an active component of OK-432, a streptococcal agent, via Toll-like receptor 4 in human head and neck cancer cell lines.

    abstract::Toll-like receptor 4 (TLR4) plays a significant role in cancer therapy as receptors of bacteria-derived immunotherapeutic agents such as OK-432, a streptococcal immunotherapeutic agent. In addition, recent reports demonstrated that TLRs, including TLR4, are also expressed in cancer cells as well as in immunocompetent ...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2012.02.005

    authors: Tano T,Okamoto M,Kan S,Nakashiro K,Shimodaira S,Yamashita N,Kawakami Y,Hamakawa H

    更新日期:2012-08-01 00:00:00

  • Chest radiography or chest CT plus head and neck CT versus 18F-FDG PET/CT for detection of distant metastasis and synchronous cancer in patients with head and neck cancer.

    abstract:OBJECTIVES:Current guidelines recommend chest computed tomography (CT) with locoregional CT/magnetic resonance imaging for patients with head and neck squamous cell carcinoma (HNSCC), and 18F-fluorodeoxyglucose (FDG) positron-emission tomography (PET)/CT is suggested for stage III-IV patients. However, whole body scree...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2018.11.026

    authors: Kim Y,Roh JL,Kim JS,Lee JH,Choi SH,Nam SY,Kim SY

    更新日期:2019-01-01 00:00:00

  • Oncological safety of submental island flap for reconstruction of pathologically node-negative and node-positive T1-2 oral squamous cell carcinoma-related defects: A retrospective study and comparison of outcomes.

    abstract:OBJECTIVES:To evaluate the oncological safety and reliability of the submental island flap (SIF) technique in patients with pathologically node-negative (pN0) and node-positive (pN+) T1-2 oral squamous cell carcinoma (OSCC) undergoing surgical tumor resection and concurrent SIF reconstruction. PATIENTS AND METHODS:Ret...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2019.104507

    authors: Wang J,Tan Y,Shen Y,Lv M,Li J,Sun J

    更新日期:2020-03-01 00:00:00

  • Expression of vascular endothelial growth factor A, B, C, and D in oral squamous cell carcinoma.

    abstract::Vascular endothelial growth factor (VEGF) A is known to play an important role in tumor angiogenesis. The additional members of the VEGF family, VEGF B, C and D have been discovered. VEGF C and D show some selectivity toward lymphatic endothelial cells. However, whether VEGF family members play a role in tumor angioge...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/s1368-8375(03)00127-1

    authors: Shintani S,Li C,Ishikawa T,Mihara M,Nakashiro K,Hamakawa H

    更新日期:2004-01-01 00:00:00

  • Is alcohol responsible for more intra-oral cancer?

    abstract::The role of the major risk factors, tobacco smoking and alcohol consumption, on trends in intra-oral cancer mortality in England and Wales between 1911 and 1990 was investigated, using lung cancer and liver cirrhosis as surrogate markers for smoking and drinking. Standardised mortality data on lung cancer and liver ci...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/s1368-8375(00)00020-8

    authors: Hindle I,Downer MC,Moles DR,Speight PM

    更新日期:2000-07-01 00:00:00

  • miR-29b suppresses proliferation, migration, and invasion of tongue squamous cell carcinoma through PTEN-AKT signaling pathway by targeting Sp1.

    abstract:OBJECTIVES:miR-29b has been implicated in various cancers. However, the role of miR-29b in tongue squamous cell carcinoma (TSCC) remains unclear. This study aimed to investigate the role of miR-29b in TSCC progression. MATERIALS AND METHODS:The expression of miR-29b was analyzed in TSCC tissues and cells. Functional s...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2014.07.010

    authors: Jia LF,Huang YP,Zheng YF,Lyu MY,Wei SB,Meng Z,Gan YH

    更新日期:2014-11-01 00:00:00

  • Effect of photodynamic therapy using 5-aminolevulinic acid on 4-nitroquinoline-1-oxide-induced premalignant and malignant lesions of mouse tongue.

    abstract::A new photosensitizing agent 5-aminolevulinic acid (ALA)-based photodynamic therapy (PDT) has been demonstrated as a useful method for treatment of superficial neoplastic lesions. The aim of this study was to determine the effects of topically and systemically ALA-based PDT in neoplastic lesions of the oral cavity. Pr...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/s1368-8375(98)00066-9

    authors: Ma G,Ikeda H,Inokuchi T,Sano K

    更新日期:1999-01-01 00:00:00

  • Polymorphism of angiotensin I-converting enzyme gene is related to oral cancer and lymph node metastasis in male betel quid chewers.

    abstract:OBJECTIVES:Angiotensin I-converting enzyme (ACE), a type I cell surface zinc metallopeptidase, is differentially expressed in several malignancies and plays a role in tumor cell proliferation, tumor cell migration, angiogenesis, and metastatic behavior. We aimed to investigate the effects of ACE gene (rs1799752) varian...

    journal_title:Oral oncology

    pub_type: 杂志文章

    doi:10.1016/j.oraloncology.2012.06.003

    authors: Liu YT,Lin LW,Chen CY,Wang CP,Liu HP,Houng JY,Chung FM,Shieh TY

    更新日期:2012-12-01 00:00:00