Abstract:
BACKGROUND:Nonalcoholic fatty liver disease (NAFLD) consists of nonalcoholic fatty liver (NAFL) and nonalcoholic steatohepatitis (NASH); the latter progresses to liver cirrhosis and hepatocellular carcinoma. Discriminating NASH from NAFL typically involves liver biopsy. The mechanism of NASH progression is unclear but may involve immunological pathways. In this study, we examined expression levels of cytokine- and chemokine-encoding genes in peripheral blood mononuclear cells (PBMCs) from NAFLD patients and established immunological criteria for discriminating NASH from NAFL. METHODS:PBMCs were obtained from 54 patients diagnosed histologically with NAFLD (NAFL, 18; NASH, 36). mRNA was extracted from PBMCs, and expression levels of cytokine- and chemokine-encoding genes were determined by quantitative real-time PCR. Statistical analysis was performed by nonparametric test. RESULTS:Expression levels of interferon (IFN)γ, interleukin (IL)2, IL15, C-C-motif chemokine ligand (CCL)2, IL10, and C-X-C-motif chemokine ligand (CXCL)11 were significantly upregulated in NASH patients compared with NAFL patients. Moreover, their expression levels were positively correlated with the degree of ballooning of hepatocytes but not of steatosis or lobular inflammation. We focused on those encoding IL10, IFNγ, and CCL2, and developed a scoring system to discriminate NASH from NAFL. The discriminatory power of the criteria was validated in an independent cohort. CONCLUSIONS:Expression levels of the cytokine- and chemokine-encoding genes in PBMCs were positively correlated with ballooning, suggesting their utility for the diagnosis of NASH. The data indicate that peripheral as well as intrahepatic immunity is involved in the progression of NASH. Our findings afford new insight into immunological mechanisms of NASH and will facilitate its noninvasive diagnosis.
journal_name
J Gastroenteroljournal_title
Journal of gastroenterologyauthors
Kado A,Tsutsumi T,Enooku K,Fujinaga H,Ikeuchi K,Okushin K,Moriya K,Yotsuyanagi H,Koike Kdoi
10.1007/s00535-019-01565-xsubject
Has Abstractpub_date
2019-08-01 00:00:00pages
730-741issue
8eissn
0944-1174issn
1435-5922pii
10.1007/s00535-019-01565-xjournal_volume
54pub_type
杂志文章abstract:BACKGROUND AND AIMS:Insulin-like growth factor (IGF)-I receptor (IGF-IR) signaling plays important parts in both the tumorigenicity and progression of digestive/gastrointestinal malignancies. In this study, we sought to test the effectiveness of a practical approach to blocking IGF-IR signaling using RNA interference d...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-009-0151-6
更新日期:2010-02-01 00:00:00
abstract::The histopathological changes due to chronic Helicobacter pylori infection are well characterized. Nevertheless, the clinical and pathological outcomes resulting from the cure of this infection remain incompletely described. In particular, the influence of eradication of H. pylori on nonulcer dyspepsia, the long-term ...
journal_title:Journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.1007/BF02990094
更新日期:2002-01-01 00:00:00
abstract::Chronic pancreatitis is a chronic clinical disorder characterized by irreversible damage to the pancreas and the development of histologic evidence of inflammation and fibrosis, and eventually the destruction and permanent loss of exocrine and endocrine tissue. A nationwide survey in Japan revealed an increase in the ...
journal_title:Journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.1007/s005350300058
更新日期:2003-01-01 00:00:00
abstract::We report a case of synchronous gas gangrene and spontaneous bacterial peritonitis associated with liver cirrhosis. The patient was a 52-year-old man who was being followed for decompensated liver cirrhosis. He experienced sudden onset lower abdominal pain with distension and pain in the left leg. A bullous lesion, wi...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/BF02936380
更新日期:1997-04-01 00:00:00
abstract::We report a patient who survived total colonic type ulcerative colitis (UC) complicated by toxic megacolon (TM), disseminated intravascular coagulation (DIC), methicillin-resistant Staphylococcus aureus infection, and phlebothrombosis. A 69-year-old man was treated for about 4 months under the diagnosis of ischemic co...
journal_title:Journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.1007/s005350050282
更新日期:1999-06-01 00:00:00
abstract:BACKGROUND:Polymorphisms in the gene for interleukin 1beta (IL-1B) have been found to increase the risks of gastric cancer and its precursors in response to Helicobacter pylori infection in white populations. however, there has been no independent confirmation of the role of IL-1B markers in gastric cancer patients fro...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-003-1315-4
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:DNA microarray technology has enabled genomewide analysis of gene transcript levels, yielding insight into the molecular nature of liver disease. METHODS:We compared gene expression of liver biopsy specimens in 16 patients with different stages of chronic hepatitis B, five with autoimmune hepatitis (AIH), f...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-008-2237-y
更新日期:2008-01-01 00:00:00
abstract::One complication of choledochal cyst in adulthood is the development of secondary carcinoma, usually well-differentiated adenocarcinoma. We report a case of extrahepatic bile duct carcinoma associated with choledochal cyst and presenting predominantly sarcomatous features. The patient was a 52-year-old female who pres...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/BF02365455
更新日期:1994-10-01 00:00:00
abstract::To elucidate the role played by hepatic sinusoidal cells in the regulation of the circulatory status in the liver, the effect of endothelins (ETs) on primary-cultured stellate cells was examined. Kinetic analysis with 125I-labeled ET-1 revealed that stellate cells have ET receptors with a Kd value of 141 pM and a Bmax...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/BF02349639
更新日期:1995-12-01 00:00:00
abstract::Cytosolic phospholipase A2 (PLase A2) is activated by low Ca2+ concentrations and translocates from the cytosol to the cell membrane, releasing arachidonic acid; the arachidonic acid cascade then leads to the production of many inflammatory mediators. The aim of this study, accordingly, was to investigate the role of ...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s005350050215
更新日期:1999-02-01 00:00:00
abstract:BACKGROUND:For patients with chronic hepatitis due to hepatitis B virus (HBV), factors predicting hepatocellular carcinoma (HCC) other than high levels of HBV-DNA and alanine aminotransferase (ALT) are needed to prevent HCC development, as many patients with chronic HBV infection fulfill these conditions. The purpose o...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-010-0266-9
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND:Liver cirrhosis affects the results of many of the traditional techniques currently used to evaluate nutritional status. Our aim was to compare the traditional two-compartment model (subjective global assessment and anthropometry and blood tests) of nutritional assessment with a multicompartmental model (bod...
journal_title:Journal of gastroenterology
pub_type: 评论,杂志文章
doi:10.1007/s00535-006-1794-1
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND:This hospital-based analysis was conducted to identify prognostic factors of hepatocellular carcinoma (HCC) in a hepatitis B virus endemic area. METHODS:A total of 6381 HCC cases, diagnosed from 1986 to 2002, were enrolled, and 2890 (42.3%) of them were not treated. Survival rates were analyzed by correlati...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-007-2134-9
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Whether mucosal inflammation affects gastric motility in patients with functional dyspepsia (FD) is controversial. Few reports discuss gastric motility in relation to histologic gastritis. We examined the relation between gastric motility and histologic gastritis in Japanese patients with FD. METHODS:Subjec...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-008-2172-y
更新日期:2008-01-01 00:00:00
abstract::The present study was carried out to examine the effects of submucosal administration of endothelin on gastric mucosal integrity in rats. Injection of endothelin-3 into the submucosal space of the stomach induced gastric mucosal damage dose-dependently and site-specifically. The gastric injury was localized only at th...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/BF02936360
更新日期:1997-04-01 00:00:00
abstract::From its pattern of metastasis and recurrence, gastric carcinoma can be recognized as a slow-growing malignancy whose local control is still the most important part of treatment. With application of asymptomatic population screening and awareness of the high risk of this disease among the general population in Japan, ...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:Endoscopic transpapillary brush cytology and forceps biopsy are widely used for the pathological diagnosis of suspected malignant biliary strictures (MBS). However, the sensitivity of these methods remains insufficient, and it can be difficult to confirm the diagnosis. We aimed to evaluate the diagnostic abi...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-011-0404-z
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:The T-helper (Th)1 immune reaction is essential for the eradication of hepatitis C virus (HCV) during interferon (IFN) therapy in patients with chronic hepatitis C. Osteopontin is a cytokine crucial for the initiation of the Th1 response. Recently, we identified four single-nucleotide polymorphisms (SNPs) in...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-005-1558-3
更新日期:2005-04-01 00:00:00
abstract::A new triple therapy using a proton pump inhibitor and two antibiotics shows high efficiency against Helicobacter pylori infection. The aim of this study was to determine the optimal dose and duration of lansoprazole (LA) administration in combination with amoxicillin (AMPC) and metronidazole (MNZ). A total of 91 pati...
journal_title:Journal of gastroenterology
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Subunit A of coagulation factor XIII (FXIII-A) is important for clot stability and acts in the subsequent wound healing process. Loss of plasma FXIII-A has been reported after surgery, sepsis, and inflammatory conditions. In the intestinal mucosa, FXIII-A is expressed by macrophages and cellular FXIII-A has ...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-015-1152-2
更新日期:2016-08-01 00:00:00
abstract::A case of granular cell tumor occurring in the sigmoid colon is reported. The patient, a 56-year-old man, visited our hospital for further evaluation of occult blood in his stool. Endoscopic examination revealed a yellowish, hemispheric submucosal tumor (SMT) with redness, about 6 mm in diameter, in the sigmoid colon....
journal_title:Journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.1007/s005350300068
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:The remnant stomach corresponds to the gastric cardia, but is exposed to a completely different environment. The present study was performed to investigate the role of Epstein-Barr virus (EBV) infection in patients with gastric remnant carcinoma (GRC). METHODS:Clinicopathological features, gastritis, and in...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-005-1590-3
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND:The transcription factor nuclear factor-E2-related factor-2 (Nrf2) inhibits lipid accumulation and oxidative stress in the liver by interfering with lipogenic pathways and inducing antioxidative stress genes. METHODS:The involvement of Nrf2 in defense against the development of steatohepatitis was studied i...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-012-0659-z
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:It is controversial whether patients with gastric marginal zone lymphomas of mucosa associated lymphoid tissue (MALT) have higher risk of second malignancies. The aim of this study was to define the risk of second malignancies in these patients. METHODS:We analyzed prospective follow-up data of 146 consecut...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-013-0844-8
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:Cold snare polypectomy (CSP) has not undergone sufficient histopathological evaluation. This study aimed to clarify the histopathological features of CSP specimens, including resection depth and layer, as compared with endoscopic mucosal resection (EMR). METHODS:Polyps were recruited retrospectively. Sessil...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-018-1446-2
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Mucosal breaks induced by gastroesophageal reflux of gastric contents were more frequently found on the right anterior wall of the lower esophagus. Bleeding from esophageal varices may be also derived from gastroesophageal reflux. The circumferential location of the ruptured esophageal varices was evaluated ...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-008-2232-3
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Endoscopic sphincterotomy (ES) is widely performed in patients with common bile duct stones (CBDS). However, the long-term outcomes of patients following ES have not been sufficiently elucidated. Impaired papillary function following ES may result in additional late complications. In contrast, endoscopic pap...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-012-0707-8
更新日期:2013-09-01 00:00:00
abstract::We reviewed reports on the efficacy of clarithromycin in eradicating Helicobacter pylori. Reports on dual and 1-week triple therapy that included clarithromycin were analyzed to assess the eradication rates of H. pylori infection. Dual therapy with clarithromycin and a proton pump inhibitor achieved eradication rates ...
journal_title:Journal of gastroenterology
pub_type: 杂志文章,评审
doi:
更新日期:1996-11-01 00:00:00
abstract::To determine the role of endogenous substance P in ethanol-induced mucosal damage, two experiments were performed. In the first experiment, the stomachs of anesthetized rats were doubly cannulated and gastric damage was induced with 5ml of 30% ethanol in the gastric lumen. The damage was ameliorated by pretreatment wi...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/BF02355018
更新日期:1996-06-01 00:00:00
abstract:BACKGROUND:To further clarify the clinico-epidemiological features of autoimmune pancreatitis (AIP) in Japan, we conducted the fourth nationwide epidemiological survey. METHODS:This study consisted of two stage surveys; the number of AIP patients was estimated by the first survey and their clinical features were asses...
journal_title:Journal of gastroenterology
pub_type: 杂志文章
doi:10.1007/s00535-019-01658-7
更新日期:2020-04-01 00:00:00