Advances in CRISPR/Cas9 Technology for in Vivo Translation.

abstract：:Here, we confirmed that stable expression of B-cell lymphoma-xL (Bcl-xL) in N18TG neuroglioma cells could suppress c-Jun N-terminal protein kinase (JNK) activation, nuclear fragmentation, and cell death caused by etoposide treatment. Moreover, additional overexpression of JNK1 led to partially antagonize the antiapopt...

journal_title：Biological & pharmaceutical bulletin

authors： Jeong HS,Choi HY,Choi TW,Kim BW,Kim JH,Lee ER,Cho SG

更新日期：2008-09-01 00:00:00

abstract：:In the investigation of chemical constituents in antiosteoporotic active fraction, 90% ethanol fraction of water extract of the rhizomes of Dioscorea spongiosa, twenty-two glycosides were isolated and their structures were elucidated. In order to know the role of each compound played in the fraction and to find the ex...

journal_title：Biological & pharmaceutical bulletin

authors： Yin J,Han N,Liu Z,Song S,Kadota S

更新日期：2010-01-01 00:00:00

abstract：:The discovery that angiogenesis is a key condition for the growth of a tumor beyond a millimeter or two, brings about a new approach in the treatment of tumors using drugs able to inhibit the formation of new blood vessels. Also, it has been realized that antiangiogenic drugs can be useful in the treatment of other pa...

journal_title：Biological & pharmaceutical bulletin

authors： González RP,Leyva A,Moraes MO

更新日期：2001-10-01 00:00:00

abstract：:Mangiferin, a natural glucosyl xanthone from the leaves of Mangifera indica L., was previously shown to exert potent hypouricemic effects associated with inhibition of the activity of xanthine dehydrogenase/oxidase. The present study aimed to evaluate its uricosuric effect and possible molecular mechanisms underlying ...

journal_title：Biological & pharmaceutical bulletin

authors： Yang H,Gao L,Niu Y,Zhou Y,Lin H,Jiang J,Kong X,Liu X,Li L

更新日期：2015-01-01 00:00:00

abstract：:Diosgenyl saponins are the most abundant steroid saponins, and exert a large variety of biological functions. In a previous report, we showed that dioscin was able to induce cytotoxicity and apoptosis in human myeloblast leukemia HL-60 cells. This study further investigated the action mechanisms underlying this effect...

journal_title：Biological & pharmaceutical bulletin

authors： Liu MJ,Wang Z,Ju Y,Zhou JB,Wang Y,Wong RN

更新日期：2004-07-01 00:00:00

abstract：:Proteasome inhibitors are a novel class of molecular-targeted anti-cancer drugs that suppress the degradation of malfolded proteins, trigger endoplasmic reticulum (ER) stress, and activate apoptosis signals. Glucose-regulated protein 78 (GRP78), a major ER chaperone, is one of the most important molecules for transduc...

journal_title：Biological & pharmaceutical bulletin

authors： Matsumura K,Sakai C,Kawakami S,Yamashita F,Hashida M

更新日期：2014-01-01 00:00:00

abstract：:Cilnidipine is a 1,4-dihydropyridine-derived voltage-dependent calcium channel (VDCC) blocker and suppresses N-type VDCC currents in addition to L-type VDCC currents. An earlier investigation has suggested that intrathecally injected cilnidipine produces antinociception by blocking N-type VDCCs in mice. The present st...

journal_title：Biological & pharmaceutical bulletin

authors： Koganei H,Shoji M,Iwata S

更新日期：2009-10-01 00:00:00

abstract：:Recently we showed significance of biosurfactants in the field of non-viral vectors for gene transfection. There, a biosurfactant, mannosylerythritol lipid A (MEL-A), especially increased the efficiency of gene transfection mediated with cationic liposomes. However, the molecular mechanism has not been well-understood...

journal_title：Biological & pharmaceutical bulletin

authors： Ueno Y,Hirashima N,Inoh Y,Furuno T,Nakanishi M

更新日期：2007-01-01 00:00:00

abstract：:By means of glycyrrhizin (GL)-affinity and Mono S column chromatographies (HPLC), at least four GL-binding proteins (p25, p17, p15-1 and p15-2) in the two Superdex fractions (P-II and P-III fractions) from Habu snake venom were selectively purified. By determination of their N-terminal partial amino acid sequences, a ...

journal_title：Biological & pharmaceutical bulletin

authors： Ohtsuki K,Abe Y,Shimoyama Y,Furuya T,Munakata H,Takasaki C

更新日期：1998-06-01 00:00:00

abstract：:Checkpoint kinase 1 (Chk1) plays important roles in genotoxic stress-induced cell cycle checkpoint and in normal cell cycle progression. Here, we show that Chk1 is cleaved in the treatment of apoptotic dose of etoposide (ETP) or cisplatin (CIS) but not of viable dose in HeLa S3 cells. The cleavage of Chk1 was complete...

journal_title：Biological & pharmaceutical bulletin

authors： Okita N,Kudo Y,Tanuma S

更新日期：2007-02-01 00:00:00

abstract：:We studied the expression in Pseudomonas aeruginosa of an erythromycin-resistance (EMr) determinant that included the mphA gene for macrolide 2'-phosphotransferase I and originated in Escherichia coli. A recombinant plasmid, pTZ3609, that consisted of the EMr determinant and a broad-host-range vector RSF1010, endowed ...

journal_title：Biological & pharmaceutical bulletin

authors： Noguchi N,Katayama J

更新日期：1998-02-01 00:00:00

abstract：:The gastric mucus is an important factor in the gastric mucosal protection from acid, pepsin and various reagents (alcohol, aspirin, etc.). MUC5AC is the mucin secreted from surface mucous cells, and belongs to the gel-forming mucin. We examined the regulation of rat MUC5AC (rMUC5AC) mRNA by glucocorticoid in vivo and...

journal_title：Biological & pharmaceutical bulletin

authors： Tanaka T,Kobayashi T,Sunaga K,Tani S

更新日期：2001-06-01 00:00:00

abstract：:Collagen-induced arthritis (CIA) is an experimental model of rheumatoid arthritis (RA) used for studying the clinical, immunological and genetic factors of the disease. Many studies of genetic susceptibility to CIA have been performed, usually with two strains of mice, DBA/1 and B10.RIII, since they are highly suscept...

journal_title：Biological & pharmaceutical bulletin

authors： Hida S,Nagi-Miura N,Adachi Y,Ohno N

更新日期：2006-06-01 00:00:00

abstract：:An efficient protocol for the in vitro germination and propagation of Larrea divaricata CAV. (Jarilla) was established. To determine the effect of different growth regulators on the growth rates and phenol production, apical-node microshoots from in vitro germinated plantlets were incubated on the following media: 1) ...

journal_title：Biological & pharmaceutical bulletin

authors： Palacio L,Baeza MC,Cantero JJ,Cusidó R,Goleniowski ME

更新日期：2008-12-01 00:00:00

abstract：:We studied the effects of twelve 5,7-dihydroxyflavone analogs on adipogenesis in 3T3-L1 cells. Among the compounds, luteolin, diosmetin, and chrysoeriol partly inhibited adipogenesis by blocking the accumulation of triacylglycerol in the cells. Conversely, tricetin facilitated triacylglycerol accumulation in the cells...

journal_title：Biological & pharmaceutical bulletin

authors： Nishina A,Ukiya M,Fukatsu M,Koketsu M,Ninomiya M,Sato D,Yamamoto J,Kobayashi-Hattori K,Okubo T,Tokuoka H,Kimura H

更新日期：2015-01-01 00:00:00

abstract：:In this study the anti-proliferative effect of curcumin (curcuma longa) that is the active ingredient of ground dried rhizome has been studied against three local and three reference leishmanial strains, Leishmania major, Leishmania tropica and Leishmania infantum (Pakistani isolate). Curcumin has shown an average IC5...

journal_title：Biological & pharmaceutical bulletin

authors： Saleheen D,Ali SA,Ashfaq K,Siddiqui AA,Agha A,Yasinzai MM

更新日期：2002-03-01 00:00:00

abstract：:Studies have shown that angiotensin-converting enzyme 2 (ACE2) plays modulating roles in lung pathophysiology, including pulmonary fibrosis (PF) and acute lung injury. Pulmonary fibrosis is a common complication in these interstitial lung diseases, and PF always has a poor prognosis and short survival. To date, there ...

journal_title：Biological & pharmaceutical bulletin

authors： Hao Y,Liu Y

更新日期：2016-01-01 00:00:00

abstract：:We demonstrate that glycyrrhizin (GL) enhanced Fas-mediated apoptotic body formation and DNA fragmentation in T cell lines although GL alone did not induce apoptosis. The enhancement effect of Fas-mediated apoptosis by GL was dose-dependent above 0.3 microM. Time course study revealed that simultaneous co-treatment of...

journal_title：Biological & pharmaceutical bulletin

authors： Ishiwata S,Nakashita K,Ozawa Y,Niizeki M,Nozaki S,Tomioka Y,Mizugaki M

更新日期：1999-11-01 00:00:00

abstract：:Effects of supplemented docosahexaenoic acid (DHA), given as diacylglycerol (DG) rich in DHA (DHA-DG), triacylglycerol (TG) rich in DHA (DHA-TG) or fish oil concentrate (DHA-70), on the serum concentration of TG and its bioavailability in the rats with diet-induced hyperlipidemia were studied. Hypertriglyceridemia was...

journal_title：Biological & pharmaceutical bulletin

authors： Tamai T,Murota I,Maruyama K,Baba T,Toyama T,Watanabe N,Kudo N,Kawashima Y

更新日期：2007-12-01 00:00:00

abstract：:Direct detection of nitric oxide (NO) is essential for understanding the precise mechanism of its production from endothelial cells. Previously, we developed an NO detection system based on the chemiluminescence reaction between NO and luminol-H2O2. Here, we have applied this system to cultured endothelial cells for t...

journal_title：Biological & pharmaceutical bulletin

authors： Kurumatani H,Kikuchi K,Nagano T,Hirobe M,Yamazaki J,Nagao T

更新日期：1998-12-01 00:00:00

abstract：:The skeletal renin–angiotensin system (RAS) is involved in the progression of osteoporosis and the active peptide within the RAS, angiotensin II (ANG II), has deleterious effects on bones. This study was performed to investigate whether suppression of the rate-limiting step of the RAS cascade by the renin inhibitor al...

journal_title：Biological & pharmaceutical bulletin

authors： Zhang FY,Yang FJ,Yang JL,Wang L,Zhang Y

更新日期：2014-01-01 00:00:00

abstract：:The correlation between the steady-state level of inducible nitric oxide synthase (iNOS) mRNA and skin tumors induced following treatment with 12-o-tetradecanoylphorbol-13-acetate (TPA) was investigated in transgenic TG-AC mice, which carry the v-Ha-ras oncogene fused to the promoter of the mouse embryonic alpha-like,...

journal_title：Biological & pharmaceutical bulletin

authors： Lee BM,Kim HS,Jeohn GH

更新日期：2000-07-01 00:00:00

abstract：:This study aims to determine the potential renal protective effects of Opuntia ficus-indica (L.) Miller (OFI) fruits against cisplatin-induced nephrotoxicity in mice. The antioxidant activity of OFI methanol extract was calculated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfo...

journal_title：Biological & pharmaceutical bulletin

authors： Okur ME,Ayla Ş,Karadağ AE,Çiçek Polat D,Demirci S,Seçkin İ

更新日期：2020-01-01 00:00:00

abstract：:Chronic fatigue (CF) is a common reason for consulting a physician due to affecting quality of life, but only a few effective treatments are available. The aim of this study was to examine the effectiveness of subcutaneous injection of the human placental extract (HPE) on medically indescribable cases of CF and safety...

journal_title：Biological & pharmaceutical bulletin

authors： Park SB,Kim KN,Sung E,Lee SY,Shin HC

更新日期：2016-05-01 00:00:00

abstract：:We investigated whether poly-L-arginine (PLA) could improve permeation of the hydrophilic compounds, FITC-labeled dextran (MW 3800, FD-4) and pyridoxamine, through ocular surface tissues. Samples of cornea, conjunctiva, or conjunctiva/sclera composite from Japanese white rabbits were mounted in Ussing chambers to meas...

journal_title：Biological & pharmaceutical bulletin

authors： Nemoto E,Takahashi H,Kobayashi D,Ueda H,Morimoto Y

更新日期：2006-01-01 00:00:00

abstract：:The hypoglycemic and hypolipidemic effect of docosahexaenoic acid (DHA; C22: 6omega-3) ethyl ester was examined in KK-Ay mice and neonatal streptozotocin-induced diabetic (NSZ) which are respectively obese and lean animal models of non-insulin-dependent diabetes mellitus (NIDDM), and in ddY normal mice. Single adminis...

journal_title：Biological & pharmaceutical bulletin

authors： Shimura T,Miura T,Usami M,Ishihara E,Tanigawa K,Ishida H,Seino Y

更新日期：1997-05-01 00:00:00

abstract：:The prophylactic action of scymnol in a rat peripheral arterial occlusion model, involving injection of 5% lactic acid into the femoral artery, was investigated. Increases in serum lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) activities and in plasma leve...

journal_title：Biological & pharmaceutical bulletin

authors： Ishida H,Nakayasu H,Nukaya H,Tsuji K

更新日期：1999-08-01 00:00:00

abstract：:Methylsulfonylmethane (MSM), also known as dimethyl sulfone and methyl sulfone, is an organic sulfur-containing compound that occurs naturally in a variety of fruits, vegetables, grains, and animals, including humans. In the present study, we demonstrated the anti-inflammatory effects of MSM in lipopolysaccharide (LPS...

journal_title：Biological & pharmaceutical bulletin

authors： Kim YH,Kim DH,Lim H,Baek DY,Shin HK,Kim JK

更新日期：2009-04-01 00:00:00

abstract：:To search for possible anti-tumor-promoters, we carried out a primary screening of 21 cucurbitane triterpenoids using an in vitro assay system. Of these triterpenoids, scandenoside R6 (6), 23,24-dihydrocucurbitacin F (14), 25-acetyl-23,24-dihydrocucurbitacin F (15), 2-O-beta-D-glucopyranosyl-23,24-dihydrocucurbitacin ...

journal_title：Biological & pharmaceutical bulletin

authors： Konoshima T,Takasaki M,Tatsumoto T,Kozuka M,Kasai R,Tanaka O,Nie RL,Tokuda H,Nishino H,Iwashima A

更新日期：1994-05-01 00:00:00

abstract：:Inhibition of Rho kinase (ROCK) ameliorates many cardiovascular dysfunctions. The aim of the current study is to investigate the anti-inflammatory effects of fasudil, a selective ROCK inhibitor, on high cholesterol diet-induced hypercholesterolemic rats and its possible mechanisms. In hypercholesterolemic rats, we fou...

journal_title：Biological & pharmaceutical bulletin

authors： Ma Z,Zhang J,Du R,Ji E,Chu L

更新日期：2011-01-01 00:00:00