Abstract:
:Gene expression may occur in unexpected ectopic sites when diverse genetic elements are juxtaposed as chimeric genes in transgenic mice. To determine the specific contribution of the promoter and reporter gene in ectopic expression, we have analyzed the expression of 14 different fusion genes in transgenic mice. Chimeric genes containing the mouse metallothionein-I promoter linked to either the rat or human growth hormone gene or the calcitonin/CGRP gene are expressed in a very similar pattern of neuronal regions. This ectopic expression is not a unique feature of the metallothionein promoter, since transferring the human growth hormone gene to four other heterologous promoters resulted in varying degrees of ectopic expression in overlapping subsets of cortical and hypothalamic neurons. The novel pattern of ectopic expression suggests that these otherwise unrelated neurons share a common developmental regulatory machinery for activation of gene transcription.
journal_name
Neuronjournal_title
Neuronauthors
Russo AF,Crenshaw EB 3rd,Lira SA,Simmons DM,Swanson LW,Rosenfeld MGdoi
10.1016/0896-6273(88)90079-7subject
Has Abstractpub_date
1988-06-01 00:00:00pages
311-20issue
4eissn
0896-6273issn
1097-4199pii
0896-6273(88)90079-7journal_volume
1pub_type
杂志文章相关文献
NEURON文献大全abstract::The carboxyl terminus of presenilin 1 and 2 (PS1 and PS2) binds to the neuron-specific cell adhesion molecule telencephalin (TLN) in the brain. PS1 deficiency results in the abnormal accumulation of TLN in a yet unidentified intracellular compartment. The first transmembrane domain and carboxyl terminus of PS1 form a ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(01)00512-8
更新日期:2001-11-20 00:00:00
abstract::We recorded from single neurons in the hippocampus and entorhinal cortex (EC) of rats to investigate the role of these structures in navigation and memory representation. Our results revealed two novel phenomena: first, many cells in CA1 and the EC fired at significantly different rates when the animal was in the same...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)00018-0
更新日期:2000-07-01 00:00:00
abstract::Astrocytes invade the developing retina from the optic nerve head, over the axons of retinal ganglion cells (RGCs). RGCs express the platelet-derived growth factor A-chain (PDGF-A) and retinal astrocytes the PDGF alpha-receptor (PDGFR alpha), suggesting that PDGF mediates a paracrine interaction between these cells. T...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80244-5
更新日期:1996-12-01 00:00:00
abstract::Neurotrophins and tyrosine receptor kinase (Trk) receptors are expressed in skeletal muscle, but it is unclear what functional role Trk-mediated signaling plays during postnatal life. Full-length TrkB (trkB.FL) as well as truncated TrkB (trkB.t1) were found to be localized primarily to the postsynaptic acetylcholine r...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)81113-7
更新日期:1999-11-01 00:00:00
abstract::The Fragile X mental retardation-1 (Fmr1) gene encodes a multifunctional protein, FMRP, with intrinsic RNA binding activity. We have developed an approach, antibody-positioned RNA amplification (APRA), to identify the RNA cargoes associated with the in vivo configured FMRP messenger ribonucleoprotein (mRNP) complex. U...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(03)00034-5
更新日期:2003-02-06 00:00:00
abstract::In the brain, information is encoded by the firing patterns of neuronal ensembles and the strength of synaptic connections between individual neurons. We report here that representation of the environment by "place" cells is altered by changing synaptic weights within hippocampal networks. Long-term potentiation (LTP)...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(03)00465-3
更新日期:2003-08-28 00:00:00
abstract::A fundamental question about the neural correlates of attention concerns the earliest sensory processing stage that it can affect. We addressed this issue by recording magnetoencephalography (MEG) signals while subjects performed detection tasks, which required employment of spatial or nonspatial attention, in auditor...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2008.04.013
更新日期:2008-06-12 00:00:00
abstract::Abnormally phosphorylated tau proteins (A68) are the building blocks of Alzheimer's disease (AD) paired helical filaments. The biological consequences of the conversion of normal adult tau to A68 remain unknown. Here we demonstrate that native A68 does not bind to microtubules (MTs), yet dephosphorylated A68 regains t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90057-x
更新日期:1993-06-01 00:00:00
abstract::Circadian clocks consist of transcriptional feedback loops housed in interdependent pacemaker neurons. Yet little is known about the neuronal output components essential for rhythmic behavior. Drosophila mutants of a putative ion channel, narrow abdomen (na), exhibit poor circadian rhythms and suppressed daylight acti...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2005.10.030
更新日期:2005-12-22 00:00:00
abstract::Although pain is defined as a sensory and emotional experience, it is traditionally researched and clinically treated separately from emotion. Conceptual and mechanistic relationships between these constructs highlight the need for better understanding of their bi-directional influences and the value of bridging the p...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/j.neuron.2020.05.024
更新日期:2020-07-08 00:00:00
abstract::Insulin-like growth factor I (IGF-I) is a potent regulator of oligodendrocyte development and myelination in vitro, but its effect on myelination in vivo has never been tested directly. Therefore, we examined brain growth and myelination in a transgenic mouse line that overexpresses IGF-I. By postnatal day 55, when br...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(93)90173-o
更新日期:1993-04-01 00:00:00
abstract::Somatic mutation of the MTOR gene is a genetic etiology of focal malformations of cortical development. In this issue of Neuron, Park et al. (2018) identify defective autophagy-dependent ciliogenesis/Wnt signaling as an underlying mechanism affecting neuronal migration and cortical lamination. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2018.06.042
更新日期:2018-07-11 00:00:00
abstract::We have identified factors that control precise motor timing by studying learning in smooth pursuit eye movements. Monkeys tracked a target that moved horizontally for a fixed time interval before changing direction through the addition of a vertical component of motion. After repeated presentations of the same target...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2004.12.017
更新日期:2005-01-06 00:00:00
abstract::Membrane depolarization causes voltage-gated ion channels to transition from a resting/closed conformation to an activated/open conformation. We used voltage-clamp fluorometry to measure protein motion at specific regions of the Shaker Kv channel. This enabled us to construct new structural models of the resting/close...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2007.09.023
更新日期:2007-10-04 00:00:00
abstract::During development, newly born neurons migrate away from their initial birth sites to their final positions in the mature brain. The neurotrophin BDNF has been shown to regulate the migration of granule cells in the cerebellum. The cellular mechanisms that mediate this chemotactic response have not been resolved. In t...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2007.06.025
更新日期:2007-07-05 00:00:00
abstract::We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocampal slices, NrbI is concentrated in dendritic spines by binding...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2004.09.022
更新日期:2004-10-14 00:00:00
abstract::Dopamine neurons in the ventral tegmental area (VTA) play an important role in the motivational systems underlying drug addiction, and recent work has suggested that they also release the excitatory neurotransmitter glutamate. To assess a physiological role for glutamate corelease, we disrupted the expression of vesic...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2010.02.012
更新日期:2010-03-11 00:00:00
abstract::Both the sec6/8 complex and septin filaments have been implicated in directing vesicles and proteins to sites of active membrane addition in yeast. The rat brain sec6/8 complex coimmunoprecipitates with a filament composed of four mammalian septins, suggesting an interaction between these complexes. One of the septins...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80493-6
更新日期:1998-06-01 00:00:00
abstract::Homomeric assembly of Kir5.1, an inward-rectifying K+ channel subunit, is believed to be nonfunctional, although the subunit exists abundantly in the brain. We show that HEK293T cells cotransfected with Kir5.1 and PSD-95 exhibit a Ba(2+)-sensitive inward-rectifying K+ current. Kir5.1 coexpressed with PSD-95 located on...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(02)00675-x
更新日期:2002-04-25 00:00:00
abstract::Homeostatic signaling systems are thought to interface with the mechanisms of neural plasticity to achieve stable yet flexible neural circuitry. However, the time course, molecular design, and implementation of homeostatic signaling remain poorly defined. Here we demonstrate that a homeostatic increase in presynaptic ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2006.09.029
更新日期:2006-11-22 00:00:00
abstract::Phorbol esters and purified protein kinase C (PKC) have been shown to down-modulate the voltage-dependent Na+ channels expressed in Xenopus oocytes injected with chick brain RNA. We used the two-electrode voltage-clamp technique to demonstrate that a Na+ channel expressed in oocytes injected with RNA coding for the al...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90131-i
更新日期:1991-01-01 00:00:00
abstract::Nervous system injury and disease have broad effects on the functional connectivity of the nervous system, but how injury signals are spread across neural circuits remains unclear. We explored how axotomy changes the physiology of severed axons and adjacent uninjured "bystander" neurons in a simple in vivo nerve prepa...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2020.11.012
更新日期:2020-12-02 00:00:00
abstract::Mouse vision is based on the parallel output of more than 30 functional types of retinal ganglion cells (RGCs). Little is known about how representations of visual information change between retina and dorsolateral geniculate nucleus (dLGN) of the thalamus, the main relay between retina and cortex. Here, we functional...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.01.040
更新日期:2019-04-17 00:00:00
abstract::Brief bath application of N-methyl-D-aspartate (NMDA) to hippocampal slices produces long-term synaptic depression (LTD) in CA1 that is (1) sensitive to postnatal age, (2) saturable, (3) induced postsynaptically, (4) reversible, and (5) not associated with a change in paired pulse facilitation. Chemically induced LTD ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80632-7
更新日期:1998-11-01 00:00:00
abstract::At each of the brain's vast number of synapses, the presynaptic nerve terminal, synaptic cleft, and postsynaptic specialization form a transcellular unit to enable efficient transmission of information between neurons. While we know much about the molecular machinery within each compartment, we are only beginning to u...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/j.neuron.2017.10.006
更新日期:2017-11-01 00:00:00
abstract::Phosphorylation of brain Na+ channels by protein kinase C (PKC) decreases peak Na+ current and slows macroscopic inactivation, but receptor-activated modulation of Na+ currents via the PKC pathway has not been demonstrated. We have examined modulation of Na+ channels by activation of muscarinic receptors in acutely-is...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80125-7
更新日期:1996-05-01 00:00:00
abstract::Music can be seen as a model system for understanding gene × environment interactions and how these can influence neurocognitive development. The concept of musicality, however, is underspecified and not well understood. Here, I propose a framework for defining musicality to provide a foundation for studying the contr...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2012.01.017
更新日期:2012-02-23 00:00:00
abstract::We describe recent progress toward defining neuronal cell types in the mouse retina and attempt to extract lessons that may be generally useful in the mammalian brain. Achieving a comprehensive catalog of retinal cell types now appears within reach, because researchers have achieved consensus concerning two fundamenta...
journal_title:Neuron
pub_type: 杂志文章,评审
doi:10.1016/j.neuron.2014.08.054
更新日期:2014-09-17 00:00:00
abstract::To identify the molecular mechanisms underlying psychostimulant-elicited plasticity in the brain reward system, we undertook a phenotype-driven approach using genome-wide microarray profiling of striatal transcripts from three genetic and one pharmacological mouse models of psychostimulant or dopamine supersensitivity...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(04)00048-0
更新日期:2004-02-19 00:00:00
abstract::The dopaminergic D3 receptor (D3R) is emerging as an antagonist of sensitized behavior induced by addictive drugs such as cocaine. New work by Wang and coworkers in this issue of Neuron now identifies CaMKII as an important negative regulator of the D3R. CaMKII relieves the D3R-mediated inhibition on sensitized behavi...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2009.01.025
更新日期:2009-02-12 00:00:00