Neuronal expression of chimeric genes in transgenic mice.

Abstract:

:Gene expression may occur in unexpected ectopic sites when diverse genetic elements are juxtaposed as chimeric genes in transgenic mice. To determine the specific contribution of the promoter and reporter gene in ectopic expression, we have analyzed the expression of 14 different fusion genes in transgenic mice. Chimeric genes containing the mouse metallothionein-I promoter linked to either the rat or human growth hormone gene or the calcitonin/CGRP gene are expressed in a very similar pattern of neuronal regions. This ectopic expression is not a unique feature of the metallothionein promoter, since transferring the human growth hormone gene to four other heterologous promoters resulted in varying degrees of ectopic expression in overlapping subsets of cortical and hypothalamic neurons. The novel pattern of ectopic expression suggests that these otherwise unrelated neurons share a common developmental regulatory machinery for activation of gene transcription.

journal_name

Neuron

journal_title

Neuron

authors

Russo AF,Crenshaw EB 3rd,Lira SA,Simmons DM,Swanson LW,Rosenfeld MG

doi

10.1016/0896-6273(88)90079-7

subject

Has Abstract

pub_date

1988-06-01 00:00:00

pages

311-20

issue

4

eissn

0896-6273

issn

1097-4199

pii

0896-6273(88)90079-7

journal_volume

1

pub_type

杂志文章

相关文献

NEURON文献大全
  • Interaction with telencephalin and the amyloid precursor protein predicts a ring structure for presenilins.

    abstract::The carboxyl terminus of presenilin 1 and 2 (PS1 and PS2) binds to the neuron-specific cell adhesion molecule telencephalin (TLN) in the brain. PS1 deficiency results in the abnormal accumulation of TLN in a yet unidentified intracellular compartment. The first transmembrane domain and carboxyl terminus of PS1 form a ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(01)00512-8

    authors: Annaert WG,Esselens C,Baert V,Boeve C,Snellings G,Cupers P,Craessaerts K,De Strooper B

    更新日期:2001-11-20 00:00:00

  • Trajectory encoding in the hippocampus and entorhinal cortex.

    abstract::We recorded from single neurons in the hippocampus and entorhinal cortex (EC) of rats to investigate the role of these structures in navigation and memory representation. Our results revealed two novel phenomena: first, many cells in CA1 and the EC fired at significantly different rates when the animal was in the same...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(00)00018-0

    authors: Frank LM,Brown EN,Wilson M

    更新日期:2000-07-01 00:00:00

  • PDGF mediates a neuron-astrocyte interaction in the developing retina.

    abstract::Astrocytes invade the developing retina from the optic nerve head, over the axons of retinal ganglion cells (RGCs). RGCs express the platelet-derived growth factor A-chain (PDGF-A) and retinal astrocytes the PDGF alpha-receptor (PDGFR alpha), suggesting that PDGF mediates a paracrine interaction between these cells. T...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(00)80244-5

    authors: Fruttiger M,Calver AR,Krüger WH,Mudhar HS,Michalovich D,Takakura N,Nishikawa S,Richardson WD

    更新日期:1996-12-01 00:00:00

  • Disruption of Trkb-mediated signaling induces disassembly of postsynaptic receptor clusters at neuromuscular junctions.

    abstract::Neurotrophins and tyrosine receptor kinase (Trk) receptors are expressed in skeletal muscle, but it is unclear what functional role Trk-mediated signaling plays during postnatal life. Full-length TrkB (trkB.FL) as well as truncated TrkB (trkB.t1) were found to be localized primarily to the postsynaptic acetylcholine r...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(00)81113-7

    authors: Gonzalez M,Ruggiero FP,Chang Q,Shi YJ,Rich MM,Kraner S,Balice-Gordon RJ

    更新日期:1999-11-01 00:00:00

  • RNA cargoes associating with FMRP reveal deficits in cellular functioning in Fmr1 null mice.

    abstract::The Fragile X mental retardation-1 (Fmr1) gene encodes a multifunctional protein, FMRP, with intrinsic RNA binding activity. We have developed an approach, antibody-positioned RNA amplification (APRA), to identify the RNA cargoes associated with the in vivo configured FMRP messenger ribonucleoprotein (mRNP) complex. U...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(03)00034-5

    authors: Miyashiro KY,Beckel-Mitchener A,Purk TP,Becker KG,Barret T,Liu L,Carbonetto S,Weiler IJ,Greenough WT,Eberwine J

    更新日期:2003-02-06 00:00:00

  • Place representation within hippocampal networks is modified by long-term potentiation.

    abstract::In the brain, information is encoded by the firing patterns of neuronal ensembles and the strength of synaptic connections between individual neurons. We report here that representation of the environment by "place" cells is altered by changing synaptic weights within hippocampal networks. Long-term potentiation (LTP)...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(03)00465-3

    authors: Dragoi G,Harris KD,Buzsáki G

    更新日期:2003-08-28 00:00:00

  • Attention modulates earliest responses in the primary auditory and visual cortices.

    abstract::A fundamental question about the neural correlates of attention concerns the earliest sensory processing stage that it can affect. We addressed this issue by recording magnetoencephalography (MEG) signals while subjects performed detection tasks, which required employment of spatial or nonspatial attention, in auditor...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2008.04.013

    authors: Poghosyan V,Ioannides AA

    更新日期:2008-06-12 00:00:00

  • Abnormal tau phosphorylation at Ser396 in Alzheimer's disease recapitulates development and contributes to reduced microtubule binding.

    abstract::Abnormally phosphorylated tau proteins (A68) are the building blocks of Alzheimer's disease (AD) paired helical filaments. The biological consequences of the conversion of normal adult tau to A68 remain unknown. Here we demonstrate that native A68 does not bind to microtubules (MTs), yet dephosphorylated A68 regains t...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/0896-6273(93)90057-x

    authors: Bramblett GT,Goedert M,Jakes R,Merrick SE,Trojanowski JQ,Lee VM

    更新日期:1993-06-01 00:00:00

  • The ion channel narrow abdomen is critical for neural output of the Drosophila circadian pacemaker.

    abstract::Circadian clocks consist of transcriptional feedback loops housed in interdependent pacemaker neurons. Yet little is known about the neuronal output components essential for rhythmic behavior. Drosophila mutants of a putative ion channel, narrow abdomen (na), exhibit poor circadian rhythms and suppressed daylight acti...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2005.10.030

    authors: Lear BC,Lin JM,Keath JR,McGill JJ,Raman IM,Allada R

    更新日期:2005-12-22 00:00:00

  • What Is the Relationship between Pain and Emotion? Bridging Constructs and Communities.

    abstract::Although pain is defined as a sensory and emotional experience, it is traditionally researched and clinically treated separately from emotion. Conceptual and mechanistic relationships between these constructs highlight the need for better understanding of their bi-directional influences and the value of bridging the p...

    journal_title:Neuron

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuron.2020.05.024

    authors: Gilam G,Gross JJ,Wager TD,Keefe FJ,Mackey SC

    更新日期:2020-07-08 00:00:00

  • Insulin-like growth factor I increases brain growth and central nervous system myelination in transgenic mice.

    abstract::Insulin-like growth factor I (IGF-I) is a potent regulator of oligodendrocyte development and myelination in vitro, but its effect on myelination in vivo has never been tested directly. Therefore, we examined brain growth and myelination in a transgenic mouse line that overexpresses IGF-I. By postnatal day 55, when br...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/0896-6273(93)90173-o

    authors: Carson MJ,Behringer RR,Brinster RL,McMorris FA

    更新日期:1993-04-01 00:00:00

  • mTOR'ing across the Cortex by Chopping the Cilia.

    abstract::Somatic mutation of the MTOR gene is a genetic etiology of focal malformations of cortical development. In this issue of Neuron, Park et al. (2018) identify defective autophagy-dependent ciliogenesis/Wnt signaling as an underlying mechanism affecting neuronal migration and cortical lamination. ...

    journal_title:Neuron

    pub_type: 评论,杂志文章

    doi:10.1016/j.neuron.2018.06.042

    authors: Di Nardo A,Sahin M

    更新日期:2018-07-11 00:00:00

  • The representation of time for motor learning.

    abstract::We have identified factors that control precise motor timing by studying learning in smooth pursuit eye movements. Monkeys tracked a target that moved horizontally for a fixed time interval before changing direction through the addition of a vertical component of motion. After repeated presentations of the same target...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2004.12.017

    authors: Medina JF,Carey MR,Lisberger SG

    更新日期:2005-01-06 00:00:00

  • Closing in on the resting state of the Shaker K(+) channel.

    abstract::Membrane depolarization causes voltage-gated ion channels to transition from a resting/closed conformation to an activated/open conformation. We used voltage-clamp fluorometry to measure protein motion at specific regions of the Shaker Kv channel. This enabled us to construct new structural models of the resting/close...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2007.09.023

    authors: Pathak MM,Yarov-Yarovoy V,Agarwal G,Roux B,Barth P,Kohout S,Tombola F,Isacoff EY

    更新日期:2007-10-04 00:00:00

  • Developing with BDNF: a moving experience.

    abstract::During development, newly born neurons migrate away from their initial birth sites to their final positions in the mature brain. The neurotrophin BDNF has been shown to regulate the migration of granule cells in the cerebellum. The cellular mechanisms that mediate this chemotactic response have not been resolved. In t...

    journal_title:Neuron

    pub_type: 评论,杂志文章

    doi:10.1016/j.neuron.2007.06.025

    authors: Kaplan DR,Miller FD

    更新日期:2007-07-05 00:00:00

  • Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton.

    abstract::We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocampal slices, NrbI is concentrated in dendritic spines by binding...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2004.09.022

    authors: Zito K,Knott G,Shepherd GM,Shenolikar S,Svoboda K

    更新日期:2004-10-14 00:00:00

  • Vesicular glutamate transport promotes dopamine storage and glutamate corelease in vivo.

    abstract::Dopamine neurons in the ventral tegmental area (VTA) play an important role in the motivational systems underlying drug addiction, and recent work has suggested that they also release the excitatory neurotransmitter glutamate. To assess a physiological role for glutamate corelease, we disrupted the expression of vesic...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2010.02.012

    authors: Hnasko TS,Chuhma N,Zhang H,Goh GY,Sulzer D,Palmiter RD,Rayport S,Edwards RH

    更新日期:2010-03-11 00:00:00

  • Subunit composition, protein interactions, and structures of the mammalian brain sec6/8 complex and septin filaments.

    abstract::Both the sec6/8 complex and septin filaments have been implicated in directing vesicles and proteins to sites of active membrane addition in yeast. The rat brain sec6/8 complex coimmunoprecipitates with a filament composed of four mammalian septins, suggesting an interaction between these complexes. One of the septins...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(00)80493-6

    authors: Hsu SC,Hazuka CD,Roth R,Foletti DL,Heuser J,Scheller RH

    更新日期:1998-06-01 00:00:00

  • PSD-95 mediates formation of a functional homomeric Kir5.1 channel in the brain.

    abstract::Homomeric assembly of Kir5.1, an inward-rectifying K+ channel subunit, is believed to be nonfunctional, although the subunit exists abundantly in the brain. We show that HEK293T cells cotransfected with Kir5.1 and PSD-95 exhibit a Ba(2+)-sensitive inward-rectifying K+ current. Kir5.1 coexpressed with PSD-95 located on...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(02)00675-x

    authors: Tanemoto M,Fujita A,Higashi K,Kurachi Y

    更新日期:2002-04-25 00:00:00

  • Mechanisms underlying the rapid induction and sustained expression of synaptic homeostasis.

    abstract::Homeostatic signaling systems are thought to interface with the mechanisms of neural plasticity to achieve stable yet flexible neural circuitry. However, the time course, molecular design, and implementation of homeostatic signaling remain poorly defined. Here we demonstrate that a homeostatic increase in presynaptic ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2006.09.029

    authors: Frank CA,Kennedy MJ,Goold CP,Marek KW,Davis GW

    更新日期:2006-11-22 00:00:00

  • Activation of protein kinase C alters voltage dependence of a Na+ channel.

    abstract::Phorbol esters and purified protein kinase C (PKC) have been shown to down-modulate the voltage-dependent Na+ channels expressed in Xenopus oocytes injected with chick brain RNA. We used the two-electrode voltage-clamp technique to demonstrate that a Na+ channel expressed in oocytes injected with RNA coding for the al...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/0896-6273(91)90131-i

    authors: Dascal N,Lotan I

    更新日期:1991-01-01 00:00:00

  • Injury-Induced Inhibition of Bystander Neurons Requires dSarm and Signaling from Glia.

    abstract::Nervous system injury and disease have broad effects on the functional connectivity of the nervous system, but how injury signals are spread across neural circuits remains unclear. We explored how axotomy changes the physiology of severed axons and adjacent uninjured "bystander" neurons in a simple in vivo nerve prepa...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2020.11.012

    authors: Hsu JM,Kang Y,Corty MM,Mathieson D,Peters OM,Freeman MR

    更新日期:2020-12-02 00:00:00

  • Mouse dLGN Receives Functional Input from a Diverse Population of Retinal Ganglion Cells with Limited Convergence.

    abstract::Mouse vision is based on the parallel output of more than 30 functional types of retinal ganglion cells (RGCs). Little is known about how representations of visual information change between retina and dorsolateral geniculate nucleus (dLGN) of the thalamus, the main relay between retina and cortex. Here, we functional...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2019.01.040

    authors: Román Rosón M,Bauer Y,Kotkat AH,Berens P,Euler T,Busse L

    更新日期:2019-04-17 00:00:00

  • NMDA induces long-term synaptic depression and dephosphorylation of the GluR1 subunit of AMPA receptors in hippocampus.

    abstract::Brief bath application of N-methyl-D-aspartate (NMDA) to hippocampal slices produces long-term synaptic depression (LTD) in CA1 that is (1) sensitive to postnatal age, (2) saturable, (3) induced postsynaptically, (4) reversible, and (5) not associated with a change in paired pulse facilitation. Chemically induced LTD ...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(00)80632-7

    authors: Lee HK,Kameyama K,Huganir RL,Bear MF

    更新日期:1998-11-01 00:00:00

  • Transcellular Nanoalignment of Synaptic Function.

    abstract::At each of the brain's vast number of synapses, the presynaptic nerve terminal, synaptic cleft, and postsynaptic specialization form a transcellular unit to enable efficient transmission of information between neurons. While we know much about the molecular machinery within each compartment, we are only beginning to u...

    journal_title:Neuron

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuron.2017.10.006

    authors: Biederer T,Kaeser PS,Blanpied TA

    更新日期:2017-11-01 00:00:00

  • Muscarinic modulation of sodium current by activation of protein kinase C in rat hippocampal neurons.

    abstract::Phosphorylation of brain Na+ channels by protein kinase C (PKC) decreases peak Na+ current and slows macroscopic inactivation, but receptor-activated modulation of Na+ currents via the PKC pathway has not been demonstrated. We have examined modulation of Na+ channels by activation of muscarinic receptors in acutely-is...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(00)80125-7

    authors: Cantrell AR,Ma JY,Scheuer T,Catterall WA

    更新日期:1996-05-01 00:00:00

  • What does it mean to be musical?

    abstract::Music can be seen as a model system for understanding gene × environment interactions and how these can influence neurocognitive development. The concept of musicality, however, is underspecified and not well understood. Here, I propose a framework for defining musicality to provide a foundation for studying the contr...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/j.neuron.2012.01.017

    authors: Levitin DJ

    更新日期:2012-02-23 00:00:00

  • Neuronal cell types and connectivity: lessons from the retina.

    abstract::We describe recent progress toward defining neuronal cell types in the mouse retina and attempt to extract lessons that may be generally useful in the mammalian brain. Achieving a comprehensive catalog of retinal cell types now appears within reach, because researchers have achieved consensus concerning two fundamenta...

    journal_title:Neuron

    pub_type: 杂志文章,评审

    doi:10.1016/j.neuron.2014.08.054

    authors: Seung HS,Sümbül U

    更新日期:2014-09-17 00:00:00

  • Identification of PSD-95 as a regulator of dopamine-mediated synaptic and behavioral plasticity.

    abstract::To identify the molecular mechanisms underlying psychostimulant-elicited plasticity in the brain reward system, we undertook a phenotype-driven approach using genome-wide microarray profiling of striatal transcripts from three genetic and one pharmacological mouse models of psychostimulant or dopamine supersensitivity...

    journal_title:Neuron

    pub_type: 杂志文章

    doi:10.1016/s0896-6273(04)00048-0

    authors: Yao WD,Gainetdinov RR,Arbuckle MI,Sotnikova TD,Cyr M,Beaulieu JM,Torres GE,Grant SG,Caron MG

    更新日期:2004-02-19 00:00:00

  • Hooked on the D3 receptor: CaMKII's new addiction.

    abstract::The dopaminergic D3 receptor (D3R) is emerging as an antagonist of sensitized behavior induced by addictive drugs such as cocaine. New work by Wang and coworkers in this issue of Neuron now identifies CaMKII as an important negative regulator of the D3R. CaMKII relieves the D3R-mediated inhibition on sensitized behavi...

    journal_title:Neuron

    pub_type: 评论,杂志文章

    doi:10.1016/j.neuron.2009.01.025

    authors: Hell JW

    更新日期:2009-02-12 00:00:00