A 13C NMR study of the application of [U-13C]succinate for metabolic investigations in rabbit renal proximal convoluted tubular cells.

Abstract:

:The fate of the 13C label arising from [U-13C]succinate in rabbit renal proximal convoluted tubular (PCT) cells, incubated with [U-13C]succinate, was investigated by 13C NMR spectroscopy of the perchloric acid extracts of the cell pellets. The 13C label of [U-13C]succinate was predominantly metabolized into glutamate, glutamine, and into glucose, lactate, and alanine, which is the consequence of a carbon efflux of the label from the Krebs cycle at the stage of alpha-ketoglutarate and oxaloacetate, respectively.

journal_name

Magn Reson Med

authors

Jans AW,Willem R

doi

10.1002/mrm.1910140114

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

148-53

issue

1

eissn

0740-3194

issn

1522-2594

journal_volume

14

pub_type

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