Abstract:
BACKGROUND:The importance of microbiota infiltrating the gut mucus layer has been reported in the pathogenesis of various gastrointestinal and systemic diseases. However, little is known about the mucosa-associated microbiota (MAM) in healthy subjects. The present study aimed to clarify the characteristics of the gastrointestinal MAM from the oral cavity to the rectum in healthy Japanese subjects. METHODS:Seventeen healthy subjects were enrolled. In this study, 5 mucosa samples from the upper gut (intraoral, mid-esophagus, gastric corpus, gastric antrum, and duodenum) and 7 from the lower gut (ileum, cecum, ascending colon, transverse colon, descending colon, sigmoid colon, and rectum) were collected with a brush under endoscopic examination. MAM profiles of each sample were analyzed by 16S-rRNA V3-V4 gene sequences. RESULTS:Collecting mucosa samples by brushing provided sufficient material for MAM profiling without causing adverse effects. The upper and lower gut MAM profiles differed significantly (p < 0.0001). In the upper and lower gut, the intra- and inter-individual MAM profiles were significantly different (p = 0.0008 and p < 0.0001 respectively). CONCLUSIONS:The MAM profiles of the upper and lower gut were significantly different. The inter-individual differences in MAM were remarkable compared to the intra-individual differences.
journal_name
Digestionjournal_title
Digestionauthors
Kashiwagi S,Naito Y,Inoue R,Takagi T,Nakano T,Inada Y,Fukui A,Katada K,Mizushima K,Kamada K,Uchiyama K,Handa O,Ishikawa T,Itoh Ydoi
10.1159/000496102subject
Has Abstractpub_date
2020-01-01 00:00:00pages
107-120issue
2eissn
0012-2823issn
1421-9867pii
000496102journal_volume
101pub_type
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