Establishment and performance assessment of preparation technology of internal quality control products for blood transfusion compatibility testing.

Abstract:

:The aim of this study was to establish and to optimize the preparation technology of whole blood internal quality control (IQC) products for blood transfusion compatibility testing. Several B-type RhD-negative blood samples collected from healthy donors were mixed. Two groups of whole blood IQC products, namely, the preservative solution group (PS group) and the saline group, were prepared. The agglutination intensity of IQC sample red cells and anti-B antibody, IgM anti-A antibody and reverse-typing A cell, IgG anti-D and O-type RhD-positive red cells, as well as free hemoglobin concentration in the supernatant of the two groups were detected. The erythrocytes in both groups were damaged to a certain extent during storage, but no evident (above moderate) hemolysis was observed in the stored sample within 42 days. The red cells remained structurally complete and the reaction activity of IgG anti-D reagent remained generally unchanged (P>0.05). Although the reaction activity oscillation of IgM anti-A reagent was observed, the agglutination intensity varied within an acceptable range of 1+. No difference was observed between the preparation methods of the samples, i.e., between the erythrocyte washed with saline and the one washed with red cell preservative solution (P>0.05). The long shelf life, low variance between tubes and stable antigen-antibody reaction activity of the whole blood IQC products prepared using the proposed method can meet the requirements of blood transfusion compatibility testing.

journal_name

Exp Ther Med

authors

Yu Y,Ma C,Feng Q,Chen X,Guan X,Zhang X,Chen L,Lin Z,Pan J,Zhang T,Luo Q,Wang D

doi

10.3892/etm.2013.994

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

1466-1470

issue

5

eissn

1792-0981

issn

1792-1015

pii

etm-05-05-1466

journal_volume

5

pub_type

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