Abstract：
:Pathogens survive and propagate within host cells through a wide array of complex interactions. Tracking the molecular and cellular events by multidimensional fluorescence microscopy has been a widespread tool for research on intracellular pathogens. Through major advancements in 3D electron microscopy, intracellular pathogens can also be visualized in their cellular environment to an unprecedented level of detail within large volumes. Recently, multidimensional fluorescence microscopy has been correlated with volume electron microscopy, combining molecular and functional information with the overall ultrastructure of infection events. In this review, we provide a short introduction to correlative focused ion beam/scanning electron microscopy (c-FIB/SEM) tomography and illustrate its utility for intracellular pathogen research through a series of studies on Shigella, Salmonella, and Brucella cellular invasion. We conclude by discussing current limitations of and prospects for this approach.
journal_name
Trends Microbioljournal_title
Trends in microbiologyauthors
Weiner A,Enninga Jdoi
10.1016/j.tim.2018.11.011subject
Has Abstractpub_date
2019-05-01 00:00:00pages
426-439issue
5eissn
0966-842Xissn
1878-4380pii
S0966-842X(18)30273-7journal_volume
27pub_type
杂志文章,评审abstract：:The polysaccharide-rich wall, which envelopes the fungal cell, is pivotal to the maintenance of cellular integrity and for the protection of the cell from external aggressors - such as environmental fluxes and during host infection. This review considers the commonalities in the composition of the wall across the fung...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2017.05.015
更新日期：2017-12-01 00:00:00
abstract：:The blood-brain barrier (BBB) provides significant protection against microbial invasion of the brain. However, the BBB is not impenetrable, and mechanisms by which viruses breach it are becoming clearer. In vivo and in vitro model systems are enabling identification of host and viral factors contributing to breakdown...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2012.03.009
更新日期：2012-06-01 00:00:00
abstract：:The discovery of 'molecular syringes' in several important gastrointestinal pathogens including Escherichia coli, Salmonella, Shigella and Yersinia, together with a better understanding of M cells and the mucosal immune system, has advanced our appreciation of multistage microorganism-host cell interactions. Recent st...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0966-842x(00)01828-x
更新日期：2000-10-01 00:00:00
abstract：:The microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) are a family of proteins that are defined by the presence of two adjacent IgG-like folded subdomains. These promote binding to ligands by mechanisms that involve major conformational changes exemplified by the binding to fibrinogen by th...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2019.06.007
更新日期：2019-11-01 00:00:00
abstract：:Many reports have documented that Staphylococcus aureus can invade host cells and persist intracellularly for various periods of time in cell culture models. However, it is not clear whether intracellular persistence of S. aureus also occurs in the course of infections in whole organisms. This is a subject of intense ...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2008.11.005
更新日期：2009-02-01 00:00:00
abstract：:The use of quality control mechanisms to stall developmental pathways or completely remove defective cells from a population is a widespread strategy to ensure the integrity of morphogenetic programs. Endospore formation (sporulation) is a well conserved microbial developmental strategy in the Firmicutes phylum wherei...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2017.03.005
更新日期：2017-08-01 00:00:00
abstract：:Persistence of the latent reservoir remains a challenge to curing HIV infection. Using shRNA screening, new insights into the molecular mechanisms underlying latency regulation indicate that the estrogen receptor is a potent repressor of proviral reactivation and may serve as a promising therapeutic target in combinat...
journal_title：Trends in microbiology
pub_type： 评论,杂志文章
doi：10.1016/j.tim.2018.09.005
更新日期：2018-11-01 00:00:00
abstract：:Protozoans of the genus Plasmodium are the causative agents of malaria; they have a complex life cycle involving vertebrate and arthropod hosts and have three distinct invasive stages. Although the invasive stages probably invade cells using similar mechanisms, each stage has a different host cell specificity and util...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0966-842x(97)84657-4
更新日期：1997-02-01 00:00:00
abstract：:Microbiology has unraveled rich evidence of ongoing reticulate evolutionary processes and complex interactions both within and between cells. These phenomena feature real biological networks, which can logically be analyzed using network-based tools. It is thus not surprising that network sciences, a field independent...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2019.11.006
更新日期：2020-04-01 00:00:00
abstract：:Phosphite, a species of phosphorus in a P3+ oxidation state, is believed to have played an important role in the primordial Earth. Figueroa et al. used metagenomics to uncover anaerobic bacterial communities from waste water waste sludge that sustain life from energy provided by phosphite. ...
journal_title：Trends in microbiology
pub_type： 评论,杂志文章
doi：10.1016/j.tim.2018.01.002
更新日期：2018-03-01 00:00:00
abstract：:Bacteria have evolved complex regulatory networks to control the activity of transcription and translation, and thus the growth rate, over a range of environmental conditions. Reengineering RNA polymerase and ribosomes allows modifying naturally evolved regulatory networks and thereby profoundly reorganizing the manne...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2016.12.009
更新日期：2017-06-01 00:00:00
abstract：:The existence of peptidoglycan (PG) in chlamydiae has long been debated. Genome sequencing of members of the Chlamydiaceae family and Protochlamydia amoebophila has uncovered a nearly complete pathway for PG synthesis in these organisms. The recent use of microarray and proteomic analysis methods has revealed that PG ...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2005.12.004
更新日期：2006-02-01 00:00:00
abstract：:Antibiotic-associated diarrhoea (AAD) is a common and unintended consequence of antibiotic use. Clostridium difficile is the most common infectious aetiology of AAD; however, only approximately 25% of all AAD cases are associated with C. difficile infection, with the aetiology in the majority of cases remaining undete...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2016.02.001
更新日期：2016-06-01 00:00:00
abstract：:Recognition of pathogenic bacteria by mammalian hosts is largely mediated by membrane-bound Toll-like receptors (TLRs). Recently, a family of cytosolic proteins, termed NODs, with homology to plant disease-resistance gene products has been implicated in sensing microbes within the cytosol. The role of NOD family membe...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2004.10.001
更新日期：2004-12-01 00:00:00
abstract：:Virulence of many bacterial pathogens is based, at least in part, on the action of phospholipases. The consequences may be immediate and direct, as in the action of Clostridium perfringens alpha toxin on red cells or platelets, or subtle, as with phosphatidylinositol-specific phospholipases of Listeria monocytogenes a...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/S0966-842X(97)01005-6
更新日期：1997-04-01 00:00:00
abstract：:A major goal in microbiology is to understand the processes by which bacteria successfully colonize host tissue. Although a wealth of studies focusing on pathogenic microorganisms has revealed much about the rare interactions that result in disease, far less is known about the regulation of the ubiquitous, long-term, ...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0966-842x(99)01588-7
更新日期：1999-10-01 00:00:00
abstract：:Most membrane-anchored histidine kinases (HKs) of bacterial two-component systems (2CSs) contain an extracellular input domain that is thought to be responsible for sensing an environmental cue. By contrast, intramembrane-sensing HKs (IM-HKs) lack a sensory domain and cannot perceive their stimuli directly. Instead, a...
journal_title：Trends in microbiology
pub_type： 杂志文章
doi：10.1016/j.tim.2014.05.006
更新日期：2014-10-01 00:00:00
abstract：:Rolling circle DNA replication is inherently continuous and unregulated. This 'go-for-broke' strategy works well for lytic phages but is suicidal for plasmids that must coexist with their host. Plasmids have consequently evolved elaborate copy number control systems that operate at the transcriptional, translational a...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/S0966-842X(97)01143-8
更新日期：1997-11-01 00:00:00
abstract：:Badgers represent a wildlife reservoir for bovine tuberculosis in Britain, which persists in the south west despite almost 20 years of badger control. The influence of landscape structure on patterns of badger urinatory behaviour may be one reason for the localized persistence of the disease in cattle within the regio...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0966-842x(94)90124-4
更新日期：1994-02-01 00:00:00
abstract：:Hepatitis C virus populations in infected individuals consist of quasispecies with diverse mutations. These quasispecies have different biological properties, and the analysis of these variants has led to new interpretations of viral persistence, carcinogenesis and resistance to interferon therapy. ...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0966-842x(00)89000-9
更新日期：1995-11-01 00:00:00
abstract：:During cellular entry and infection, the parvovirus capsid follows a complex path from the cell surface to the nucleus, where the DNA is replicated. Various receptors have been characterized that bind to different parvoviruses and mediate their entry into cells. However, the subsequent trafficking pathways within the ...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2008.01.012
更新日期：2008-05-01 00:00:00
abstract：:The fungus Candida albicans is a benign member of the mucosal microbiota, but can cause mucosal infections and life-threatening disseminated invasive infections in susceptible individuals. The ability to switch between yeast, pseudohyphal, and hyphal growth forms (polymorphism) is one of the most investigated virulenc...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2014.09.001
更新日期：2014-12-01 00:00:00
abstract：:The following pair of articles, the first by Gil Segal and Howard Shuman, and the second by James Kirby and Ralph Isberg (Trends Microbiol. 6, 256-258), explore the genetics and function of the icm/dot genes of Legionella pneumophila. This gene family is implicated in several aspects of virulence and appears to consti...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0966-842x(98)01308-0
更新日期：1998-07-01 00:00:00
abstract：:Despite being a curable disease, tuberculosis (TB) killed more people in 2009 than during any previous year in history. Progress in TB research has been slow, and remains burdened by important gaps in our knowledge of the basic biology of Mycobacterium tuberculosis, the causative agent of TB, and its interaction with ...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2011.07.002
更新日期：2011-10-01 00:00:00
abstract：:The bacterial type IV pilus (T4P) is a versatile molecular machine with a broad range of functions. Recent advances revealed that the molecular components and the biophysical properties of the machine are well conserved among phylogenetically distant bacterial species. However, its functions are diverse, and include a...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2015.09.002
更新日期：2015-12-01 00:00:00
abstract：:Growing concerns about global climate change and energy dependence have led to an increased effort to reduce carbon emissions. A considerable reduction could be achieved by using biofuels from lignocellulosic biomass instead of fossil fuels. One major bottleneck of biofuel production from lignocellulose is the availab...
journal_title：Trends in microbiology
pub_type： 杂志文章
doi：10.1016/j.tim.2008.06.001
更新日期：2008-09-01 00:00:00
abstract：:Measles virus gene expression is attenuated in neural cells by mechanisms that affect both viral transcription and translation. Host enzymes that hypermutate viral genes, and those induced by cytokines, may act cooperatively to slow viral replication and to favor persistent measles virus infections in the human centra...
journal_title：Trends in microbiology
pub_type： 杂志文章
doi：10.1016/s0966-842x(00)88976-3
更新日期：1995-09-01 00:00:00
abstract：:Two-component systems are characterized by phosphotransfer reactions involving histidine and aspartate residues in highly conserved signalling domains. Although the basic principles of signal transduction by these systems have been elucidated, several important aspects, such as their integration into more complex cell...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0966-842x(99)01458-4
更新日期：1999-03-01 00:00:00
abstract：:If we took all of the single copy genes in all sequenced genomes, made phylogenetic trees from them individually, and then made the supertree of those trees, what would we get? Recently, David Pisani and colleagues did that experiment and their results are likely to spark much discussion. Their prokaryote tree looks v...
journal_title：Trends in microbiology
pub_type： 杂志文章
doi：10.1016/j.tim.2007.09.001
更新日期：2007-10-01 00:00:00
abstract：:The recent availability of extensive metagenomic sequences from various environmental microbial communities has extended the postgenomic era to the field of environmental microbiology. Although still restricted to a small number of studies, metaproteomic investigations have revealed interesting aspects of functional g...
journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2005.12.006
更新日期：2006-02-01 00:00:00
