Abstract：
:Isolation of Coxiella Burnetii in the standard laboratory setting is hazardous; therefore most diagnoses are based on retrospective detection of a rising antibody titer to C. burnetti. As a result, this disease is usually undiagnosed or misdiagnosed. Methods for the rapid detection of C. burnetti have now been developed that utilize specific hybridization of labeled DNA probes to nucleic acid in clinical samples. One method detects the presence of C. burnetii 16S ribosomal RNA (rRNA); another uses plasmid sequences. We have developed a probe that detects C. burnetii and one that differentiates between Coxiella strains capable of causing chronic disease and those that cause the acute form. Using these probes, C. burnetii can be identified in blood, urine, and tissue samples. The plasmid-derived probes detect as few as 10(4) organisms and less than 1 ng of Coxiella DNA. A third method differentiates between chronic (endocarditis-causing) strains and those that cause acute Q fever. This method uses the polymerase chain reaction (PCR), in which the target regions of DNA are amplified by iterative cycles of Taq I DNA polymerase chain extension to produce up to a 10(6) amplification of the target sequences. When Southern blotting is used in conjunction with PCR, the test detects as few as 2-9 C. burnetti cells.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Frazier ME,Mallavia LP,Samuel JE,Baca OGdoi
10.1111/j.1749-6632.1990.tb42253.xsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
445-58eissn
0077-8923issn
1749-6632journal_volume
590pub_type
杂志文章abstract：:Taking into account that methamphetamine (MA) is a popular recreational drug among young adult women, i.e., in gestational age, the present model aims to assess the effects of its exposure during development. In this experimental model, MA effects are assessed in the rat during the first month of life, regarding both ...
journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
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更新日期：1987-01-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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更新日期：2011-02-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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doi：10.1111/j.1749-6632.1997.tb56884.x
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journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.1999.tb08684.x
更新日期：1999-10-20 00:00:00
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journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.1998.tb09602.x
更新日期：1998-05-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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doi：10.1111/j.1749-6632.1975.tb29173.x
更新日期：1975-06-30 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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doi：10.1111/j.1749-6632.1975.tb53345.x
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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更新日期：2018-12-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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更新日期：2003-12-01 00:00:00
abstract：:To identify molecular genetic markers of resistance or susceptibility to dermatophilosis in cattle, we used a functional candidate gene approach to analyze the DNA polymorphisms of targeted genes encoding molecules implicated in known mechanisms of both nonspecific and specific immune responses existing in the pathoge...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
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更新日期：2002-10-01 00:00:00
