Abstract:
:The need for quantitative molecular methods is growing in environmental, food, and medical fields but is hindered by low and variable DNA extraction and by co-extraction of PCR inhibitors. DNA extracts from Enterococcus faecium, seawater, and seawater spiked with E. faecium and Vibrio parahaemolyticus were tested by qPCR for target recovery and inhibition. Conventional and novel methods were tested, including Synchronous Coefficient of Drag Alteration (SCODA) and lysis and purification systems used on an automated genetic sensor (the Environmental Sample Processor, ESP). Variable qPCR target recovery and inhibition were measured, significantly affecting target quantification. An aggressive lysis method that utilized chemical, enzymatic, and mechanical disruption enhanced target recovery compared to commercial kit protocols. SCODA purification did not show marked improvement over commercial spin columns. Overall, data suggested a general need to improve sample preparation and to accurately assess and account for DNA recovery and inhibition in qPCR applications.
journal_name
Mar Pollut Bulljournal_title
Marine pollution bulletinauthors
Cox AM,Goodwin KDdoi
10.1016/j.marpolbul.2013.06.006subject
Has Abstractpub_date
2013-08-15 00:00:00pages
47-56issue
1eissn
0025-326Xissn
1879-3363pii
S0025-326X(13)00296-8journal_volume
73pub_type
杂志文章abstract::The present estimates of ice drift in the Arctic include utilization of satellite imagery data (special sensor microwave/imager) and a reconstruction of air pressure for the period 1899-1998. A significant part of the sea ice in the Arctic Ocean has its origin in the Kara Sea and melts in the Greenland and the Barents...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/s0025-326x(02)00087-5
更新日期:2002-09-01 00:00:00
abstract::Phytoplankton entrained into cooling water systems of coastal power stations are subjected to acute chemical stress due to biocides (chlorine) used for biofouling control. They are subsequently released into the environment, where they may survive/recover or succumb. Experiments were conducted to evaluate the suscepti...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2016.12.059
更新日期:2017-11-30 00:00:00
abstract::The ability to remotely detect and monitor oil spills is becoming increasingly important due to the high demand of oil-based products. Indeed, shipping routes are becoming very crowded and the likelihood of oil slick occurrence is increasing. In this frame, a fully integrated remote sensing system can be a valuable mo...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2015.07.045
更新日期:2016-01-30 00:00:00
abstract::This study aims to investigate the effects of ammonia nitrogen loading rates and salinity levels on nutrients removal rates and substrate enzyme activities of constructed wetland (CW) microcosms planted with Salicornia bigelovii treating mariculture wastewater. Activities of urease (UA), dehydrogenase (DA), protease (...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2018.04.013
更新日期:2018-06-01 00:00:00
abstract::This study reports on a sediment toxicity test developed with a native amphipod strain of Ptilohyale barbicornis where sediments from 10 estuarine stations along the western coastline of Taiwan were evaluated with respect to trace metals and PAHs. The test was validated by determining dose-response relationships for a...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2020.111797
更新日期:2020-11-09 00:00:00
abstract::A twelve year (2000-2011) study of three coastal lagoons in the Gulf of Mexico was conducted to assess the impacts of local watershed development and tropical storms on water quality. The lagoons have similar physical and hydrological characteristics, but differ substantially in the degree of watershed urban developme...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2017.06.038
更新日期:2017-09-15 00:00:00
abstract::To investigate the influence of mangrove reforestation on heavy metal accumulation and speciation in intertidal sediments, core sediments from a restored mangrove forest and adjacent mud flat in Yifeng Estuary (southeastern China) were analyzed. The chemical speciation of heavy metals (Pb, Zn, Cu, Cr and Ni) was deter...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2010.03.010
更新日期:2010-08-01 00:00:00
abstract::We evaluated the impact of ocean acidification on the early development of sea cucumber Apostichopus japonicus. The effect of pH-levels (pH 8.04, 7.85, 7.70 and 7.42) were tested on post-fertilization success, developmental (stage duration) and growth rates. Post-fertilization success decreased linearly with pH leadin...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2015.04.021
更新日期:2015-06-15 00:00:00
abstract::This paper focuses on destructive fishing practices in coastal Tanzania. I argue that the dominant conservation narrative that increased capacity and 'political will' for enforcement of marine protected areas (MPAs) reflects a form of spatial fetishization, in that it overemphasizes the influence of space over social ...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2018.06.008
更新日期:2018-08-01 00:00:00
abstract::The use of seabirds as sentinels of the condition of aquatic ecosystems has been well-established. Large environmental perturbations to aquatic food webs (e.g., chemical contamination, overfishing, particulate pollution) have all been detected or monitored by tracking seabirds at colonies. However, seabirds may elicit...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2009.08.024
更新日期:2010-01-01 00:00:00
abstract::Temporal trends in mercury concentrations ([Hg]) during the last two to three decades were determined in liver of shorthorn sculpin, ringed seal and Atlantic walrus from northwest Greenland (NWG, 77 degrees N) and in liver of shorthorn sculpin and ringed seal from central west Greenland (CWG, 69 degrees N) during the ...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2006.08.046
更新日期:2007-01-01 00:00:00
abstract::Seasonal variation in trace metal contamination in surface sediments was studied through high resolution profiles assessed monthly by DGT probes in muddy sediments of the North Sea. General parameters such as pH, temperature, dissolved oxygen and sulfides were also recorded to estimate their role in the speciation of ...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2014.07.053
更新日期:2014-10-15 00:00:00
abstract::The ecological role of intertidal microphytobenthos (MPB) is increasingly recognized in coastal production systems. MPB primary production (PP) measured in coastal wetlands of Korea, Cambodia, and Australia confirmed large variability at the global scale. Surprisingly, MPB biomass in mangrove forests almost doubled th...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2019.110707
更新日期:2020-02-01 00:00:00
abstract::Superhydrophobic metal wire mesh (SMWM) has frequently been applied for the selective and efficient separation of oil/water mixture due to its porous structure and special wettability. However, current methods for the modification of metal wire mesh to be superhydrophobic suffered from problems with respect to complex...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2016.09.010
更新日期:2016-12-15 00:00:00
abstract::Sources and transport processes of As, Cu, Cd and Pb were studied in different environmental compartments of Deception Island, an active volcano in the South Shetland Islands, Antarctica. Element concentrations in fresh water samples are consistent with the lowest values reported elsewhere in Antarctica. Interestingly...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2013.08.037
更新日期:2013-12-15 00:00:00
abstract::Seawater samples were collected in the lagoon of Nouméa (New Caledonia) along two transects from coastal bays to the oligotrophic barrier reef. Dissolved (<0.2 microm) and sub-micrometer (0.2-0.8 microm) concentrations of chromium, nickel and zinc were measured. Concentrations in the 0.2-0.8 microm size range were cor...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2009.07.017
更新日期:2009-12-01 00:00:00
abstract::Antibiotic use is a well-described practice to promote animal health whether for prevention or treatment. Nonetheless, it can also cause a number of potentially harmful effects that dictate the need to implement regulation to assure a reduction of hazards to the consumers and the environment. Chloramphenicol (CAP) is ...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2014.12.007
更新日期:2015-02-15 00:00:00
abstract::Our study incorporated a comprehensive suite of parameters (i.e., body size, age, diet and trophic position) to investigate mercury concentration in dusky groupers Epinephelus marginatus. This study was carried out in rocky bottoms in littoral and neritic habitats along the Southern Brazilian coast. We also determined...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2016.12.006
更新日期:2017-02-15 00:00:00
abstract::Oil weathering is often described subjectively after a spill. Adjectives like "moderate" and "severe" help define the extent of oil loss but fail to communicate quantitatively and reproducibly the degree of weathering. The use of subjective weathering terms often leads to misperceptions about persistence and toxicity ...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2020.111091
更新日期:2020-05-01 00:00:00
abstract::A habitat approach was promoted in the framework of ecological status assessment of transitional waters, assuming the importance of habitat heterogeneity to the overall system status. The approach was applied to the use of fish-based multimetric indices by adapting them to seagrass and marsh habitats in the Venice lag...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2009.06.016
更新日期:2009-11-01 00:00:00
abstract::In June 2015, an individual of Diretmichthys parini (Post and Quero, 1981) was trawled at 530m depth, in the North Sea off Norway and donated to research. This capture, the first for this species in the North Sea was the northernmost recorded so far, and provided an opportunity to document some aspects of the biology ...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2016.10.074
更新日期:2017-02-15 00:00:00
abstract::Sampling of the smoke plumes from the BP Deepwater Horizon surface oil burns led to the unintentional collection of soot particles on the sail of an instrument-bearing, tethered aerostat. This first-ever plume sampling from oil burned at an actual spill provided an opportunistic sample from which to characterize the p...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2016.03.069
更新日期:2016-06-15 00:00:00
abstract::Understanding population dynamics of established invasive species is important for designing effective management measures and predicting factors such as invasiveness and ecological impact. The kelp Undaria pinnatifida has spread to most temperate regions of the world, however a basic understanding of population dynam...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2018.10.055
更新日期:2018-12-01 00:00:00
abstract::The Wetlands Reserve in Al Wusta Governorate on the Arabian Sea is important for internationally migrating birds and local wildlife, but data on marine litter in the reserve, and wider region, are rare. Seven beaches around the reserve were surveyed for litter in February 2020. Abundance and weight of litter were cate...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2020.111503
更新日期:2020-10-01 00:00:00
abstract::Among marine debris, monofilament fishing lines often result in negative impacts on marine organisms. We characterized marine debris and incidence of lost and discarded monofilament lines along beaches used by recreational fishers, and report the impact of lines on Kelp Gulls (Larus dominicanus) at the Bahía San Blas ...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2014.05.052
更新日期:2014-08-15 00:00:00
abstract::Ecostatus of Ulhas estuary, one of the most polluted estuaries along the industrialized and urbanized northwest coast of India, was evaluated by six widely accepted benthic indices viz. H'(log2), AMBI, M-AMBI, BENTIX, BOPA and BO2A to test their efficiency in a tropical setting. The mesohaline zone, which presented eu...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2017.05.014
更新日期:2017-07-15 00:00:00
abstract::Hydrodynamics and associated environmental processes have always been of major concern to coastal-dependent countries, such as Kuwait. This is due to the environmental impact that accompanies the economic and commercial activities along the coastal areas. In the current study, a three-dimensional numerical model is ut...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2017.11.049
更新日期:2018-02-01 00:00:00
abstract::Compared to open sea environments, there is still limited knowledge about microplastic levels in semi-enclosed systems such as coastal lagoons. This work aims to assess the levels of MPs in the waters of an urban lagoon (Bizerte lagoon, northern Tunisia) and to study the effects of environmental factors on their distr...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2020.111625
更新日期:2020-11-01 00:00:00
abstract::The intertidal harpacticoid copepod Tigriopus japonicus is an important species in the study of marine pollution. To facilitate molecular biomonitoring using T. japonicus, we constructed a T. japonicus unidirectional cDNA library using lambdaZAP expression vector, excised to pBluescript vector with the aid of helper p...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2005.02.014
更新日期:2005-01-01 00:00:00
abstract::Mobulid rays are targeted in fisheries for their branchial plates, for use in Chinese medicine. Branchial plate and muscle tissue from Mobula japanica were collected from fish markets in Sri Lanka, and muscle tissue biopsies from Manta alfredi in Australia. These were analysed for arsenic, cadmium, lead and mercury an...
journal_title:Marine pollution bulletin
pub_type: 杂志文章
doi:10.1016/j.marpolbul.2015.02.005
更新日期:2015-05-15 00:00:00