Abstract:
:BD mCCDA Clear-HT (CCHT; Nippon Becton Dickinson Company, Ltd.) , a novel chromogenic selective medium was evaluated for its superior capacity to isolate Campylobacter jejuni/ coli. When CCHT was assessed using 142 microbes including 42 Campylobacter jejuni/ coli strains, all Campylobacter strains were found to form purple-colored colonies on CCHT whereas all the other microbes failed to grow. CCTH was then compared with commercially available selective media using 100 stool samples including 40 Campylobacter positive samples. CCHT detected Campylobacter jejuni/ coli from 39 of 40 (97.5%) stool samples whereas it allowed competitive bacteria to grow as false positive colonies from 1 (1.0%) of 100 stool samples. The values of relative sensitivity (%) and specificity (%) for CCHT were 97.5 and 98.3 in this study. Our results demonstrated that CCHT had the highest detection ratio for Campylobacter jejuni/ coli and the highest inhibition ratio against competitive bacteria among all selective media compared.
journal_name
Biocontrol Scijournal_title
Biocontrol scienceauthors
Teramura H,Ogura A,Yasuda E,Furuhata Kdoi
10.4265/bio.23.229subject
Has Abstractpub_date
2018-01-01 00:00:00pages
229-234issue
4eissn
1342-4815issn
1884-0205journal_volume
23pub_type
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