Evaluation of a Novel Chromogenic Selective Medium for the Improved Detection of Campylobacter from Stool Samples.

Abstract:

:BD mCCDA Clear-HT (CCHT; Nippon Becton Dickinson Company, Ltd.) , a novel chromogenic selective medium was evaluated for its superior capacity to isolate Campylobacter jejuni/ coli. When CCHT was assessed using 142 microbes including 42 Campylobacter jejuni/ coli strains, all Campylobacter strains were found to form purple-colored colonies on CCHT whereas all the other microbes failed to grow. CCTH was then compared with commercially available selective media using 100 stool samples including 40 Campylobacter positive samples. CCHT detected Campylobacter jejuni/ coli from 39 of 40 (97.5%) stool samples whereas it allowed competitive bacteria to grow as false positive colonies from 1 (1.0%) of 100 stool samples. The values of relative sensitivity (%) and specificity (%) for CCHT were 97.5 and 98.3 in this study. Our results demonstrated that CCHT had the highest detection ratio for Campylobacter jejuni/ coli and the highest inhibition ratio against competitive bacteria among all selective media compared.

journal_name

Biocontrol Sci

journal_title

Biocontrol science

authors

Teramura H,Ogura A,Yasuda E,Furuhata K

doi

10.4265/bio.23.229

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

229-234

issue

4

eissn

1342-4815

issn

1884-0205

journal_volume

23

pub_type

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