Abstract:
:The construction of the first infectious clone JFH-1 speeds up the research on hepatitis C virus (HCV). However, Huh7 cell line was the only highly permissive cell line for HCV infection and only a few clones were fully permissive. In this study, two different fully permissive clones of Huh7 cells, Huh7.5.1 and Huh7-Lunet-CD81 (Lunet-CD81) cells were compared for their responses upon HCV infection. The virus replication level was found slightly higher in Huh7.5.1 cells than that in Lunet-CD81 cells. Viability of Huh7.5.1 cells but not of Lunet-CD81 cells was reduced significantly after HCV infection. Further analysis showed that the cell cycle of infected Huh7.5.1 cells was arrested at G1 phase. The G1/S transition was blocked by HCV infection in Huh7.5.1 cells as shown by the cell cycle synchronization analysis. Genes related to cell cycle regulation was modified by HCV infection and gene interaction analysis in GeneSpring GX in Direct Interactions mode highlighted 31 genes. In conclusion, the responses of those two cell lines were different upon HCV infection. HCV infection blocked G1/S transition and cell cycle progress, thus reduced the cell viability in Huh7.5.1 cells but not in Lunet-CD81 cells. Lunet-CD81 cells might be suitable for long term infection studies of HCV.
journal_name
Virol Sinjournal_title
Virologica Sinicaauthors
Chen H,Pei R,Chen Xdoi
10.1007/s12250-013-3342-5subject
Has Abstractpub_date
2013-08-01 00:00:00pages
202-8issue
4eissn
1674-0769issn
1995-820Xjournal_volume
28pub_type
杂志文章abstract::Laos is the only landlocked country in Southeast Asia and borders Thailand, Myanmar and Cambodia, the three countries in this region that have been hardest hit by human immunodeficiency virus (HIV). Laos has been regarded as a low-HIV-prevalence country for decades. To understand the status of HIV in Laos in recent ye...
journal_title:Virologica Sinica
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journal_title:Virologica Sinica
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doi:10.1007/s12250-016-3742-4
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journal_title:Virologica Sinica
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journal_title:Virologica Sinica
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journal_title:Virologica Sinica
pub_type: 杂志文章
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更新日期:2011-04-01 00:00:00
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journal_title:Virologica Sinica
pub_type: 杂志文章,评审
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journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-013-3348-z
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journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-017-3997-4
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journal_title:Virologica Sinica
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pub_type: 杂志文章
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journal_title:Virologica Sinica
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journal_title:Virologica Sinica
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doi:10.1007/s12250-012-3234-0
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journal_title:Virologica Sinica
pub_type: 杂志文章
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journal_title:Virologica Sinica
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journal_title:Virologica Sinica
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journal_title:Virologica Sinica
pub_type: 杂志文章,评审
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journal_title:Virologica Sinica
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journal_title:Virologica Sinica
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更新日期:2016-02-01 00:00:00
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journal_title:Virologica Sinica
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更新日期:2016-04-01 00:00:00
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journal_title:Virologica Sinica
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doi:10.1007/s12250-012-3252-y
更新日期:2012-10-01 00:00:00
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更新日期:2020-09-14 00:00:00