Uridine-cytidine kinase 2 upregulation predicts poor prognosis of hepatocellular carcinoma and is associated with cancer aggressiveness.


:Patients with advanced hepatocellular carcinoma (HCC) continue to have a dismal prognosis. Potential biomarkers to determine prognosis and select targeted therapies are urgently needed for patients with HCC. This study aimed to elucidate the role of UCK2 in HCC prognosis and tumor progression. We performed a screen of public databases to identify functional genes associated with HCC tumorigenesis, progression, and outcome. We identified uridine-cytidine kinase 2 (UCK2) as a gene of interest for further study. UCK2 promoting HCC aggressiveness was demonstrated by evaluation of clinical samples, in vitro experiments, in vivo tumorigenicity, and transcript analysis. UCK2 expression was generally elevated in HCC and was significantly correlated with poor survival and inferior clinicopathological characteristics of HCC patients. A multivariate analysis revealed that high UCK2 expression was an independent factor for poor prognosis. In HCC cell lines, UCK2 knockdown suppressed cell migration and invasion and inhibited cell proliferation, while UCK2 overexpression had an opposite effect. Animal model experiments confirmed that knockdown of UCK2 suppressed tumor growth in vivo. The bioinformatics analysis demonstrated that UCK2 might associated with metabolsim, splicesome, and adherens junction. UCK2 is highly associated with HCC malignant behavior and is a potential prognostic predictor for HCC patients in the clinic.


Mol Carcinog


Molecular carcinogenesis


Huang S,Li J,Tam NL,Sun C,Hou Y,Hughes B,Wang Z,Zhou Q,He X,Wu L




Has Abstract


2019-04-01 00:00:00












  • A high-fat diet increases angiogenesis, solid tumor growth, and lung metastasis of CT26 colon cancer cells in obesity-resistant BALB/c mice.

    abstract::We evaluated whether high-fat diet (HFD), in the absence of increased calorie intake, increases colon cancer growth and metastasis. Four-week-old male BALB/c mice were fed on an HFD (60 kcal% fat) or control diet (10 kcal% fat) for 16 wk, after which CT26 colon cancer cells were subcutaneously injected into the right ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Park H,Kim M,Kwon GT,Lim DY,Yu R,Sung MK,Lee KW,Daily JW 3rd,Park JH

    更新日期:2012-11-01 00:00:00

  • Hyperpigmentation and melanocytic hyperplasia in transgenic mice expressing the human T24 Ha-ras gene regulated by a mouse tyrosinase promoter.

    abstract::The tyrosinase promoter has been used to target expression of the mutated human T24 Ha-ras oncogene in pigment-producing cells of transgenic mice. Two independent founder mice carrying the transgene survived and showed the same distinct phenotype of mutated coat color, deeply pigmented skin with multiple nevi, and twi...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Powell MB,Hyman P,Bell OD,Balmain A,Brown K,Alberts D,Bowden GT

    更新日期:1995-02-01 00:00:00

  • Effects of supplemental calcium and vitamin D on tight-junction proteins and mucin-12 expression in the normal rectal mucosa of colorectal adenoma patients.

    abstract::The physical gut barrier, comprised of a thick mucus layer and the epithelium, plays an important role in defense against microbes and foreign antigens. Calcium and vitamin D may be involved in maintaining the integrity of the intestinal mucosal barrier, the dysfunction of which may lead to endotoxemia and inflammatio...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Mandle HB,Jahan FA,Bostick RM,Baron JA,Barry EL,Yacoub R,Merrill J,Rutherford RE,Seabrook ME,Fedirko V

    更新日期:2019-07-01 00:00:00

  • Protein phosphatase 2A is essential to maintain active Wnt signaling and its Aβ tumor suppressor subunit is not expressed in colon cancer cells.

    abstract::Canonical Wnt signaling is altered in most cases of colorectal cancer. Experimental evidence indicates that protein phosphatase 2A (PP2A) may play either positive or negative roles in Wnt signaling but its precise in vivo functions remain elusive. In this work, using colon cultured cell lines we showed that basal PP2A...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Carmen Figueroa-Aldariz M,Castañeda-Patlán MC,Santoyo-Ramos P,Zentella A,Robles-Flores M

    更新日期:2015-11-01 00:00:00

  • Estrogen-induced cell transformation and DNA adduct formation in cultured Syrian hamster embryo cells.

    abstract::To study the possible involvement of DNA damage in cell transformation induced by estrogens, we examined whether DNA adduct formation is elicited in cultured Syrian hamster embryo (SHE) cells treated with estrogens and their derivatives by means of the 32P-postlabeling assay. Morphological transformation of the cells ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Hayashi N,Hasegawa K,Komine A,Tanaka Y,McLachian JA,Barrett JC,Tsutsui T

    更新日期:1996-07-01 00:00:00

  • Protection against oxidative DNA damage and stress in human prostate by glutathione S-transferase P1.

    abstract::The pi-class glutathione S-transferase (GSTP1) actively protect cells from carcinogens and electrophilic compounds. Loss of GSTP1 expression via promoter hypermethylation is the most common epigenetic alteration observed in human prostate cancer. Silencing of GSTP1 can increase generation of reactive oxygen species (R...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kanwal R,Pandey M,Bhaskaran N,Maclennan GT,Fu P,Ponsky LE,Gupta S

    更新日期:2014-01-01 00:00:00

  • Atypical role of sprouty in p21 dependent inhibition of cell proliferation in colorectal cancer.

    abstract::Sprouty (SPRY) appears to act as a tumor suppressor in cancer, whereas we reported that SPRY2 functions as a putative oncogene in colorectal cancer (CRC) [Oncogene, 2010, 29: 5241-5253]. In general, various studies established inhibition of cell proliferation by SPRY in cancer. The mechanisms by which SPRY regulates c...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Zhang Q,Shim K,Wright K,Jurkevich A,Khare S

    更新日期:2016-09-01 00:00:00

  • Grape seed extract and resveratrol prevent 4-nitroquinoline 1-oxide induced oral tumorigenesis in mice by modulating AMPK activation and associated biological responses.

    abstract::Preventive measures against oral carcinogenesis are urgently warranted to lower the high morbidity and mortality associated with this malignancy worldwide. Here, we investigated the chemopreventive efficacy of grape seed extract (GSE) and resveratrol (Res) in 4-nitroquinoline-1-oxide (4NQO)-induced tongue tumorigenesi...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Shrotriya S,Tyagi A,Deep G,Orlicky DJ,Wisell J,Wang XJ,Sclafani RA,Agarwal R,Agarwal C

    更新日期:2015-04-01 00:00:00

  • Magnolin targeting of ERK1/2 inhibits cell proliferation and colony growth by induction of cellular senescence in ovarian cancer cells.

    abstract::Ras/Raf/MEKs/ERKs and PI3 K/Akt/mTOR signaling pathways have key roles in cancer development and growth processes, as well as in cancer malignance and chemoresistance. In this study, we screened the therapeutic potential of magnolin using 15 human cancer cell lines and combined magnolin sensitivity with the CCLE mutao...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Song JH,Lee CJ,An HJ,Yoo SM,Kang HC,Lee JY,Kim KD,Kim DJ,Lee HS,Cho YY

    更新日期:2019-01-01 00:00:00

  • Differential susceptibility to carcinogen-induced amplification of SV40 and dhfr sequences in SV40-transformed human keratinocytes.

    abstract::Gene amplification contributes to carcinogenesis by enhancing proto-oncogene activity and causing chromosomal instability. The ease of detecting amplified tumor-virus sequences has encouraged use of this system as a surrogate for studying the molecular events involved in endogenous gene amplification. We report here a...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Rossman TG,Wolosin D

    更新日期:1992-01-01 00:00:00

  • Effect of energy restriction on the expression of cyclin D1 and p27 during premalignant and malignant stages of chemically induced mammary carcinogenesis.

    abstract::The restriction of energy intake has a profound inhibitory effect on carcinogenesis, yet the mechanism or mechanisms that account for this effect are unknown. In this experiment, the hypothesis tested was that energy restriction upregulates the expression of p27/kip1, a gene product associated with cell-cycle growth a...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Zhu Z,Jiang W,Thompson HJ

    更新日期:1999-04-01 00:00:00

  • MDM2 promoter polymorphism and p53 codon 72 polymorphism in chronic myeloid leukemia: the association between MDM2 promoter genotype and disease susceptibility, age of onset, and blast-free survival in chronic phase patients receiving imatinib.

    abstract::The genetic or functional inactivation of the p53 pathway plays an important role with regards to disease progression from the chronic phase (CP) to blast phase (BP) and imatinib treatment response in chronic myeloid leukemia (CML). Two functional single nucleotide polymorphisms (SNPs), p53 R72P and MDM2 SNP309, are a...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Liu YC,Hsiao HH,Yang WC,Liu TC,Chang CS,Yang MY,Lin PM,Hsu JF,Lee CP,Lin SF

    更新日期:2014-12-01 00:00:00

  • Alsterpaullone, a novel cyclin-dependent kinase inhibitor, induces apoptosis by activation of caspase-9 due to perturbation in mitochondrial membrane potential.

    abstract::The majority of human neoplasms have aberrations in the retinoblastoma pathway due to hyperactivation of cyclin-dependent kinases (CDK). Based on this observation, novel small molecules, such as flavopiridol and UCN-01, are being developed and are currently being tested in the clinic. Efforts to develop CDK modulators...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Lahusen T,De Siervi A,Kunick C,Senderowicz AM

    更新日期:2003-04-01 00:00:00

  • Inhibition of proliferation and expression of T1 and cyclin D1 genes by thyroid hormone in mammary epithelial cells.

    abstract::The relationship between thyroid hormone (triiodothyronine, T(3)) and breast cancer is unclear. We studied the effect of the c-erbA/TR alpha proto-oncogene encoding a functional T(3) receptor (TR alpha 1), of its ligand T(3), and of its retroviral, mutated counterpart, the v-erbA oncogene, on the proliferation capacit...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: González-Sancho JM,Figueroa A,López-Barahona M,López E,Beug H,Muñoz A

    更新日期:2002-05-01 00:00:00

  • Notch3-specific inhibition using siRNA knockdown or GSI sensitizes paclitaxel-resistant ovarian cancer cells.

    abstract::Notch signaling plays an important role in ovarian cancer chemoresistance, which is responsible for recurrence. Gamma-secretase inhibitor (GSI) is a broad-spectrum Notch inhibitor, but it has serious side effects. The efficacy of Notch3-specific inhibition in paclitaxel-resistant ovarian cancers was assessed in this s...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kang H,Jeong JY,Song JY,Kim TH,Kim G,Huh JH,Kwon AY,Jung SG,An HJ

    更新日期:2016-07-01 00:00:00

  • Osteoblasts-derived TGF-beta1 enhance motility and integrin upregulation through Akt, ERK, and NF-kappaB-dependent pathway in human breast cancer cells.

    abstract::Bone metastases are common complications of breast cancer. Integrins are the major adhesive molecules in mammalian cells. Here we found that osteoblast conditioned medium (OBCM) increased the migration and cell surface expression of beta1 or beta3 integrin in human breast cancer cells (MDA-MB-231 cells). beta1 or beta...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Wei YY,Chen YJ,Hsiao YC,Huang YC,Lai TH,Tang CH

    更新日期:2008-07-01 00:00:00

  • Modulation of the activities of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin by limiting energy availability with 2-deoxyglucose.

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    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Jiang W,Zhu Z,Thompson HJ

    更新日期:2008-08-01 00:00:00

  • Harnessing the gatekeepers of glucocorticoids for chemoprevention of non-melanoma skin cancer.

    abstract::Despite effective surgical methods for non-melanoma skin cancer (NMSC), patients suffer from tissue damage, scarring, or even disfigurement; thus, there is a need for chemopreventive approaches. Because of the complex interplay between glucocorticoids (GCs), inflammation, and cancer, we sought to determine the role of...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Mancha-Ramirez AM,Yang X,Liang H,Junco J,Lee KP,Bovio SF,Espinoza M,Wool J,Slaga A,Glade DC,Hanes M,Malik G,Kim DJ,DiGiovanni J,Slaga TJ

    更新日期:2019-01-01 00:00:00

  • Epigenetic induction of tissue inhibitor of matrix metalloproteinase-3 by green tea polyphenols in breast cancer cells.

    abstract::Aberrant epigenetic silencing of the tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) gene that negatively regulates matrix metalloproteinases (MMPs) activity has been implicated in the pathogenesis and metastasis of breast cancer. In the present study, we demonstrate that green tea polyphenols (GTP) and its ma...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Deb G,Thakur VS,Limaye AM,Gupta S

    更新日期:2015-06-01 00:00:00

  • Reduced expression of the Rassf1a gene and its aberrant DNA methylation in pancreatic duct adenocarcinomas induced by N-nitrosobis(2-oxopropyl)amine in hamsters.

    abstract::Alterations of the Rassf1a gene were investigated in pancreatic duct adenocarcinomas (PDAs) induced by N-nitrosobis(2-oxopropyl)amine (BOP) in hamsters. Female Syrian golden hamsters received 70 mg/kg BOP, followed by repeated exposures to an augmentation pressure regimen consisting of a choline-deficient diet combine...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Shimizu K,Itsuzaki Y,Fujii H,Honoki K,Tsujiuchi T

    更新日期:2008-02-01 00:00:00

  • Upstream stimulatory factor (USF) as a transcriptional suppressor of human telomerase reverse transcriptase (hTERT) in oral cancer cells.

    abstract::Telomerase activity is suppressed in normal human somatic tissues but is activated in cancer cells and immortal cell lines. The reverse transcriptase (RT) subunit human telomerase reverse transcriptase (hTERT) is the key regulator of telomerase activity. The hTERT promoter contains E-box elements and may allow upstrea...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Chang JT,Yang HT,Wang TC,Cheng AJ

    更新日期:2005-11-01 00:00:00

  • Triptolide sensitizes breast cancer cells to Doxorubicin through the DNA damage response inhibition.

    abstract::Triptolide is an active component from a Chinese herb, Tripterygium wilfordii which has been applied for treating immune-related diseases over centuries. Recently, it was reported that a variety of cancer cell lines could be sensitized to DNA-damage based chemotherapy drugs in combination with Triptolide treatment. In...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Deng Y,Li F,He P,Yang Y,Yang J,Zhang Y,Liu J,Tong Y,Li Q,Mei X,Shu Z,Zhao Q

    更新日期:2018-06-01 00:00:00

  • Common and distinct mechanisms of different redox-active carcinogens involved in the transformation of mouse JB6P+ cells.

    abstract::We transformed JB6P+ cells with prolonged intermittent low-dose UVB radiation or prolonged exposure to low-dose H(2)O(2) or CdCl(2). Stable transformation was confirmed by an anchorage-independence assay. The JB6P+ transformants formed more colonies (approximately six folds) in soft agar as compared to their JB6P+ par...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Yang S,Misner B,Chiu R,Meyskens FL Jr

    更新日期:2008-07-01 00:00:00

  • Mitochondrial bioenergetic background confers a survival advantage to HepG2 cells in response to chemotherapy.

    abstract::Cancer cells mainly rely on glycolysis for energetic needs, and mitochondrial ATP production is almost inactive. However, cancer cells require the integrity of mitochondrial functions for their survival, such as the maintenance of the internal membrane potential gradient (DeltaPsim). It thus may be predicted that Delt...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Loiseau D,Morvan D,Chevrollier A,Demidem A,Douay O,Reynier P,Stepien G

    更新日期:2009-08-01 00:00:00

  • bcl-2 delay of alkylating agent-induced apoptotic death in a murine hemopoietic stem cell line.

    abstract::Many cytotoxic agents kill cells by invoking a specific death pathway termed physiological cell death, or apoptosis. Treatment of a murine hemopoietic stem cell line, FDCP-mix, with methylmethanesulfonate (MMS) or N'-methyl-N'-nitrosourea (MNU) leads to death by apoptosis. Retroviral gene transfer was used to overexpr...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Fairbairn LJ,Cowling GJ,Dexter TM,Rafferty JA,Margison GP,Reipert B

    更新日期:1994-09-01 00:00:00

  • Downregulation of STAT3/NF-κB potentiates gemcitabine activity in pancreatic cancer cells.

    abstract::There is an unmet need to develop new agents or strategies against therapy resistant pancreatic cancer (PanCA). Recent studies from our laboratory showed that STAT3 negatively regulates NF-κB and that inhibition of this crosstalk using Nexrutine® (Nx) reduces transcriptional activity of COX-2. Inhibition of these mole...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Gong J,Muñoz AR,Pingali S,Payton-Stewart F,Chan DE,Freeman JW,Ghosh R,Kumar AP

    更新日期:2017-02-01 00:00:00

  • Mutations of the p53 tumor suppressor gene and the ras gene family in intrahepatic cholangiocellular carcinomas in Japan and Thailand.

    abstract::The incidence and pattern of mutations of the ras oncogenes and the p53 tumor suppressor gene have been shown to differ among different cancer types and even among the same cancer types with different etiological backgrounds. For example, in a previous study we showed that not only the etiology but also the incidence ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kiba T,Tsuda H,Pairojkul C,Inoue S,Sugimura T,Hirohashi S

    更新日期:1993-01-01 00:00:00

  • Epigenetic regulation of the human ATP2A3 gene promoter in gastric and colon cancer cell lines.

    abstract::The knowledge about the role of calcium-regulated pathways in cancer cell growth and differentiation could be useful for the development of new therapeutic approaches to diminish its mortality. The ATP2A genes encode for SERCA pumps, which modulate cytosolic Ca2+ concentration, regulating various cellular processes in...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Meneses-Morales I,Izquierdo-Torres E,Flores-Peredo L,Rodríguez G,Hernández-Oliveras A,Zarain-Herzberg Á

    更新日期:2019-06-01 00:00:00

  • Endocrine disrupting chemicals promote the growth of ovarian cancer cells via the ER-CXCL12-CXCR4 signaling axis.

    abstract::The majority of ovarian cancers over-express the estrogen receptor (ERα) and grow in response to estrogens. We previously demonstrated that ER induction of the chemokine CXCL12 (stromal cell-derived factor-1) is required for estradiol (E2)-stimulated proliferation of human ovarian carcinoma cells. In the current study...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Hall JM,Korach KS

    更新日期:2013-09-01 00:00:00

  • Discordant effects of activator protein-1 transcription factor on gene regulation, invasion, and metastasis in spontaneous, radiation-induced, and fos-induced osteosarcomas.

    abstract::Bone cells are a prime target for the biological function of the fos/jun (activating protein-1 (AP-1)) transcription factor complex. Deregulated expression of c-fos or v-fos in bone cells induces tumorigenicity and the formation of non-metastatic osteosarcomas. In contrast, fos oncogenes transform fibroblasts to an in...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Rupp B,Lorenz U,Schmidt J,Werenskiold AK

    更新日期:1998-10-01 00:00:00