Abstract:
:Transplantation of mesenchymal stem cells (MSCs) has been extensively studied in the field of regenerative medicine. Bone regeneration is achieved via the interaction of osteoblasts and osteoclasts. However, the influence of MSCs on osteoclasts is unknown. The purpose of this study was to investigate the effect of MSCs on the expression of genes for osteoclast differentiation factors using qPCR after indirect co-culture of MSCs and RAW264 cells. The numbers of osteoclasts after addition of soluble receptor activator of nuclear factor kappa B (NF-κB) ligand (sRANKL) were also compared. Expression of osteoprotegerin (OPG) by MSCs was significantly elevated in co-culture over time. The differentiation of RAW264 cells into mature osteoclasts following addition of sRANKL was significantly inhibited by co-culture with MSCs. Expression of RANK, colony stimulating factor 1 receptor, NF-κB, and nuclear factor of activated T-cell cytoplasmic 1 in RAW264 cells was significantly inhibited by co-culture with MSCs. Expression of OPG protein was higher in co-culture with RAW264 cells than in MSCs alone, and the expression level was clearly higher than that of RANKL. MSCs appeared to inhibit osteoclast differentiation via expression of OPG.
journal_name
J Oral Scijournal_title
Journal of oral scienceauthors
Abe T,Sumi K,Kunimatsu R,Oki N,Tsuka Y,Nakajima K,Ando K,Tanimoto Kdoi
10.2334/josnusd.17-0315subject
Has Abstractpub_date
2019-03-28 00:00:00pages
30-35issue
1eissn
1343-4934issn
1880-4926journal_volume
61pub_type
杂志文章abstract::Oral submucous fibrosis (OSMF) is a disabling, potentially malignant condition of the oral cavity. The aetiology of OSMF is multifactorial but remains obscure. Although arecanut is considered to be the most important causative agent, responses observed in individuals using arecanut vary in relation to quantity and dur...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.53.97
更新日期:2011-03-01 00:00:00
abstract::Previously, we showed that recombinant human bone morphogenetic protein-2 (rhBMP-2) increased bone augmentation beyond the skeletal envelope within a titanium cap in a rabbit calvarium; many cuboidal osteoblastic cells were observed histologically. These results suggested that the new osteoblastic cells might have dif...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.50.427
更新日期:2008-12-01 00:00:00
abstract::Once a tooth develops deep caries and the dental pulp tissue is irreversibly infected, the infected dental pulp tissue should be removed, and filling material should be placed in the root canal. Endodontically treated teeth are prone to root fracture or periapical periodontitis; however, dental pulp tissue has the pot...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.17-0456
更新日期:2018-12-29 00:00:00
abstract::This study explored differences in dental biofilm solids and fluid fluoride after ingestion of NaF or Na2FPO3 in milk or non-fluoridated milk. Eighteen volunteers ingested 1 mg fluoride in 200 mL of milk or 200 mL of non-fluoridated milk. Biofilm samples were collected at baseline, 30, 60, and 240 min and biofilm soli...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.55.233
更新日期:2013-09-01 00:00:00
abstract::Ammonium hexafluorosilicate (AHF) has been applied to arrest caries without discoloration. The purpose of this study was to observe structural and elemental changes of demineralized and AHF applied primary tooth enamel. Enamel from the labial surface of 20 primary canines was divided into an unground side and ground s...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.55.115
更新日期:2013-01-01 00:00:00
abstract::Massive osteolysis is a rare, insidious, chronic disease characterized by progressive resorption of contiguous osseous structures. In 1838 Jackson first described a case of disappearing humerus. More than 150 cases have since been described in the international literature, with fewer then 35 involving a maxillofacial ...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.52.513
更新日期:2010-09-01 00:00:00
abstract::Porphyromonas gingivalis, a gram-negative anaerobe, is one of the major causative agents of periodontal disease. In this study, the effects of chlorhexidine digluconate and hydrogen peroxide on the hemin binding of P. gingivalis and coaggregation of this bacterium with oral streptococci were examined. The pretreatment...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.43.1
更新日期:2001-03-01 00:00:00
abstract::A retrospective analysis was conducted on patients diagnosed with and treated for keratocystic odontogenic tumor (KCOT) at Mashhad School of Dentistry between 1996 and 2006. The patients comprised 44 men and 30 women with a mean age of 27.08 years. Among the total of 83 lesions, 56 (67.5%) occurred in the mandible and...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.49.229
更新日期:2007-09-01 00:00:00
abstract::Glucose transporters, such as GLUT-1, mediate the important mechanisms involved in cellular glucose influx, allowing cells to proliferate and survive. The significance of GLUT-1 expression in oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC) has been less explored, and no study has investigated i...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.57.115
更新日期:2015-06-01 00:00:00
abstract::Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by recurrent thrombosis, other associated autoimmune disease, and/or obstetrical morbidity along with persistent production of antiphospholipid antibodies. Because of the nature of this systemic disease, most patients are medicated with an...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.18-0164
更新日期:2019-08-28 00:00:00
abstract::This pilot study evaluated, by culture testing, the effectiveness of lethal photosensitization for the microbiological treatment of peri-implantitis in dogs. Experimental peri-implantitis was induced by ligature placement for 2 months. Following ligature removal, plaque control was instituted by scrubbing with 0.12% c...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.45.17
更新日期:2003-03-01 00:00:00
abstract::A novel fast-setting calcium silicate cement with fluoride (CSC) has been developed for potential application in tooth crowns. This study compared the cytotoxicity of CSC compositions and a variety of dental materials. We tested CSC compositions (Protooth), MTA, Biodentine, Ketac Molar, Fuji II LC, Vitrebond, DeTrey Z...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.16-0751
更新日期:2018-01-01 00:00:00
abstract::Immunoglobulin A (IgA), which plays an important role in infection defense, is upregulated in the large intestine and oral cavity through dietary fiber intake. However, the mechanism underlying salivary IgA increase through dietary fiber intake remains unknown. This study investigated time-dependent effects of non-abs...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.16-0820
更新日期:2017-01-01 00:00:00
abstract::This study assessed push-out strength, cement layer thickness, and interfacial nanoleakage of luted fiber posts fabricated with computer-aided design/computer-assisted manufacture (CAD/CAM) technology after use of 1 of 3 scanning techniques, namely, direct scanning of the post space (DS), scanning of a polyether impre...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.17-0254
更新日期:2018-01-01 00:00:00
abstract::This study investigated the effects of indirect composite polymerization on the postcuring mechanical properties of a fiber-reinforced composite. An indirect composite seated on glass fibers preimpregnated with polymerized monomer was polymerized by 1) photoirradiation using a halogen-fluorescent polymerizing unit for...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.16-0117
更新日期:2017-03-31 00:00:00
abstract::The purpose of this study was to evaluate the presence of A. actinomycetemcomitans, P. gingivalis, P. intermedia, E. corrodens and F. nucleatum in 30 subjects with chronic periodontitis treated by scaling and root planing (SRP) plus minocycline (test group) during 12 months with regular trimester maintenance care. Add...
journal_title:Journal of oral science
pub_type: 杂志文章,随机对照试验
doi:10.2334/josnusd.50.259
更新日期:2008-09-01 00:00:00
abstract::Titanium surface characteristics, including microtopography, chemical composition, and wettability, are essential features to achieve osseointegration of dental implants, but the choice of a particular surface topography is still a debated topic among clinicians. An increased level of implant surface hydrophilicity ha...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.19-0235
更新日期:2020-03-28 00:00:00
abstract::Gingival epithelial cells and fibroblasts play important roles and have a harmonious relationship under normal and disease conditions, but the precise differences between theses cells remain unknown. To study the differences in gene expression between human gingival epithelial cells (HGE) and human gingival fibroblast...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.46.19
更新日期:2004-03-01 00:00:00
abstract::Placement of composite resin restorations in deep subgingival cavities can damage surrounding soft tissues. In addition, commonly used resin-based composites (RBCs) might interfere with wound healing and periodontal health. To clarify cellular interactions with RBCs, we used an MTT assay to investigate adhesion of pri...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.16-0827
更新日期:2018-03-24 00:00:00
abstract::It is widely accepted that vitamin E (VE) acts as an antioxidant and is involved in various metabolic systems including the regulation of gene expression and inhibition of cell proliferation. The most predominant isoform of VE in the living body is α-tocopherol. However, the influence of α-tocopherol on bone marrow me...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.17-0422
更新日期:2018-01-01 00:00:00
abstract::A rare case of primary intraosseous carcinoma (PIOC) arising from an odontogenic cyst in a 58-year-old man is reported. Clinical and radiological examinations revealed an odontogenic cyst of the maxilla. Histopathologically, the lesion was composed of a cyst with a parakeratotic epithelial lining and well-differentiat...
journal_title:Journal of oral science
pub_type: 杂志文章,评审
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更新日期:2002-03-01 00:00:00
abstract::Melatonin is produced by the pineal gland and regulates various physiological processes including osteoblast differentiation and bone formation. Bone sialoprotein (BSP) is a mineralized connective tissue-specific protein expressed in the early stage of cementum and bone mineralization. To elucidate the effects of mela...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.56.67
更新日期:2014-03-01 00:00:00
abstract::The external gonial angle is an important angle of the craniofacial complex. It is significant for the diagnosis of craniofacial disorders. Lateral cephalogram and orthopantomograms can be used to determine this angle. In this study, we compared the external gonial angle determined from the two mentioned radiographs i...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.51.425
更新日期:2009-09-01 00:00:00
abstract::The effect of therapeutic agents on trabecular bone loss in the mandible was investigated in ovariectomized rats. Eighty-seven Wistar SPF female rats were ovariectomized (OVX) or given a sham operation (Sham), and maintained on a diet containing 0.1% calcium. Four weeks later, groups of OVX rats were treated with estr...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.46.75
更新日期:2004-06-01 00:00:00
abstract::Despite its important role in the control of periodontal disease, mechanical plaque control is not properly practiced by most individuals. Therefore, adjunctive chemical plaque control using chlorhexidine and antibiotics has also been suggested as an additional therapeutic strategy to augment mechanical plaque control...
journal_title:Journal of oral science
pub_type: 杂志文章,评审
doi:10.2334/josnusd.54.1
更新日期:2012-03-01 00:00:00
abstract::When a tooth is avulsed and replanted following traumatic dental injury, complications such as replacement and inflammatory resorption may occur. Ultimately, resorption may result in loss of the tooth. This case report describes a traumatic injury to a permanent tooth resulting in complete root resorption within a sho...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.49.341
更新日期:2007-12-01 00:00:00
abstract::Since 1998, the authors have been working to improve the oral health of children at kindergartens and primary schools in the Kingdom of Tonga (Tonga). Our primary activity has been a school-based fluoride mouth-rinsing (FMR) program. FMR is performed using 7-10 mL of a 0.2% NaF solution for 1 min once per week at each...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.54.343
更新日期:2012-01-01 00:00:00
abstract::The purpose of the present study was to evaluate periodontal health status in children with sickle cell disease (SCD). Forty-nine children with SCD and 39 systemically healthy sex- and age-matched children were enrolled in the study. Plaque index, gingival index, bleeding on probing, pocket depth, salivary volume, and...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.16-0796
更新日期:2018-01-01 00:00:00
abstract::It is reported in the European and American literature that salivary duct cysts constitute about 10% of all cysts of the salivary glands, although they appear to be rare in Japan. Between 1975 and 1999, only 3 (0.5%) of 586 salivary gland cysts were diagnosed as salivary duct cysts at the Division of Clinical Patholog...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.43.9
更新日期:2001-03-01 00:00:00
abstract::The aims of this study were to investigate gender differences in frontal sinus morphology and to estimate differences between Fijian and Western Samoan populations. Cephalograms of 118 Fijians (59 male, 59 female), 80 W. Samoans (33 male, 47 female) and 89 Japanese (60 male, 29 female) were used in this study. The thi...
journal_title:Journal of oral science
pub_type: 杂志文章
doi:10.2334/josnusd.40.25
更新日期:1998-03-01 00:00:00