Abstract:
OBJECTIVE:The healing of wounds is critical in protecting the human body against environmental factors. The mechanisms involving protein expression during this complex physiological process have not been fully elucidated. APPROACH:Here, we use reverse-phase protein microarrays (RPPA) involving 94 phosphoproteins to study tissue samples from tubes implanted in healing dermal wounds in seven human subjects tracked over two weeks. We compare the proteomic profiles to proteomes of controls obtained from skin biopsies from the same subjects. MAIN RESULTS:Compared to previous proteomic studies of wound healing, our approach focuses on wound tissue instead of wound fluid, and has the sensitivity to go beyond measuring only highly abundant proteins. To study the temporal dynamics of networks involved in wound healing, we applied two network analysis methods that integrate the experimental results with prior knowledge about protein-protein physical and regulatory interactions, as well as higher-level biological processes and associated pathways. SIGNIFICANCE:We uncovered densely connected networks of proteins that are up- or down-regulated during human wound healing, as well as their relationships to microRNAs and to proteins outside of our set of targets that we measured with proteomic microarrays.
journal_name
Physiol Measjournal_title
Physiological measurementauthors
Gao X,Petricoin EF 3rd,Ward KR,Goldberg SR,Duane TM,Bonchev D,Arodz T,Diegelmann RFdoi
10.1088/1361-6579/aaee19subject
Has Abstractpub_date
2018-12-07 00:00:00pages
124002issue
12eissn
0967-3334issn
1361-6579journal_volume
39pub_type
杂志文章abstract::Virtual shunt describes the overall loss of O2 content between the alveolar gas and arterial blood. Clinicians indirectly estimate the magnitude of the virtual shunt by monitoring peripheral blood oxygen saturation (SpO2) using non-invasive pulse oximetry. An inherent limitation of this method is the variable precisio...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/36/5/967
更新日期:2015-05-01 00:00:00
abstract::The ability to predict if an atrial fibrillation (AF) episode terminates spontaneously or not through non-invasive techniques is a challenging problem of great clinical interest. This fact could avoid useless therapeutic interventions and minimize the risks for the patient. The present work introduces a robust AF pred...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/29/1/005
更新日期:2008-01-01 00:00:00
abstract::Two studies concerning multi-frequency impedance measurements are presented. The first uses tetrapolar measurements made on the thorax and the second electrical impedance tomography images, also made from the thorax. The way in which the impedance and the changes in impedance with ventilation depend upon frequency are...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/15/2a/001
更新日期:1994-05-01 00:00:00
abstract::A photoplethysmographic (PPG) signal can provide very useful information about a subject's cardiovascular status. Motion artifacts (MAs), which usually deteriorate the waveform of a PPG signal, severely obstruct its applications in the clinical diagnosis and healthcare area. To reduce the MAs from a PPG signal, in the...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/36/10/2159
更新日期:2015-10-01 00:00:00
abstract::False alarm (FA) rates as high as 86% have been reported in intensive care unit monitors. High FA rates decrease quality of care by slowing staff response times while increasing patient burdens and stresses. In this study, we proposed a rule-based and multi-channel information fusion method for accurately classifying ...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/37/8/1298
更新日期:2016-08-01 00:00:00
abstract::Analysis of human eye pupil reactivity is a very valuable diagnostic method used mainly for evaluation of the condition of the autonomic nervous system and the visual system. The paper presents an experimental pupillometer built in the Institute of Physics of the Wroclaw University of Technology. The apparatus makes i...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/28/1/006
更新日期:2007-01-01 00:00:00
abstract::During anaesthesia awareness and nociception are serious complications that may further lead to haemodynamic instability. Specific monitoring of depth of hypnosis and depth of analgesia based on heart rate variability (HRV) analysis is eligible to improve patient safety and reduce efforts in post-operative care. Conse...
journal_title:Physiological measurement
pub_type: 临床试验,杂志文章
doi:10.1088/0967-3334/33/2/207
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVE:Accurate bladder size estimation is an important clinical parameter that assists physicians, enabling them to provide better treatment for patients who are suffering from urinary incontinence. Electrical impedance tomography (EIT) is a non-invasive medical imaging method that estimates organ boundaries assumi...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/1361-6579/abaa56
更新日期:2020-12-18 00:00:00
abstract::The increasing use of EIT in clinical research on severely ill lung patients requires a clarification of the influence of pathologic impedance distributions on the validity of the resulting tomograms. Significant accumulation of low-conducting air (e.g. pneumothorax or emphysema) or well-conducting liquid (e.g. haemat...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/27/5/S16
更新日期:2006-05-01 00:00:00
abstract:OBJECTIVE:Arterial CO2 (PaCO2) has a strong effect on cerebral blood flow (CBF), but its influence on CBF regulatory mechanisms and circulatory systemic variables has not been fully described over the entire physiological range of PaCO2. APPROACH:CBF velocity (CBFV, transcranial Doppler), blood pressure (BP, Finometer...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/1361-6579/aac76b
更新日期:2018-06-19 00:00:00
abstract::Cycling on an ergometer is one effective means of measuring cardiovascular function while applying stress on the heart. Bathing in a hot water bath applies a low stress to the heart. The electrocardiograms of a healthy adult male (aged 35 at the start of study) were recorded while taking a hot water bath with no elect...
journal_title:Physiological measurement
pub_type: 临床试验,杂志文章
doi:10.1088/0967-3334/20/4/304
更新日期:1999-11-01 00:00:00
abstract::This study investigates the cerebral hemodynamic changes during a routine sleep-deprived video-electroencephalogram (SD-VEEG) in healthy children. Forty-two children with normal intelligence were examined. The children were 5-14 years of age, and their electroencephalograms (EEGs) were within the normal range. Each su...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/37/7/981
更新日期:2016-07-01 00:00:00
abstract::Electrical impedance tomography (EIT) could be used as a portable non-invasive means to image the development of ischaemic stroke or haemorrhage. The purpose of this study was to examine if this was possible using time difference imaging, in the anesthetised rat using 40 spring-loaded scalp electrodes with applied con...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/37/6/765
更新日期:2016-06-01 00:00:00
abstract::In the clinical environment the reliable interpretation of EIT images depends on the quality of the data. In the electrically noisy hospital environment the system performance needs to be assessed for each clinical investigation. From the model of noise presented, a figure of merit for comparisons of system performanc...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/17/4a/006
更新日期:1996-11-01 00:00:00
abstract::There is a lack of studies that examine the dynamics of heat-induced shrinkage of organ tissues. Clinical procedures such as radiofrequency ablation, microwave ablation or high-intensity focused ultrasound, use heat to treat diseases such as cancer and cardiac arrhythmia. When heat is applied to tissues, shrinkage occ...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/35/1/55
更新日期:2014-01-01 00:00:00
abstract::Health is one of the most important non-material assets and thus also has an enormous influence on material values, since treating and preventing diseases is expensive. The number one cause of death worldwide today originates in cardiovascular diseases. For these reasons the aim of understanding the functions and the ...
journal_title:Physiological measurement
pub_type: 杂志文章,评审
doi:10.1088/0967-3334/37/5/R46
更新日期:2016-05-01 00:00:00
abstract::This observational study investigated digital auscultation for the purpose of assessing the clinical feasibility of monitoring vascular sounds in pregnancy. The study was performed at the Regional Hospital Viborg, Denmark, and included 29 pregnant women, 10 non-pregnant women and 10 male participants. Digital ausculta...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/37/7/1163
更新日期:2016-07-01 00:00:00
abstract::The widening application of automatic external defibrillators (AEDs) presents very strong requirements for external electrocardiogram (ECG) signal analysis. Highly accurate detection of shockable rhythms is required, aimed to approach the maximum of 100% sensitivity and specificity. In a previous study the performance...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/22/2/302
更新日期:2001-05-01 00:00:00
abstract::The back-projected independent components (BICs) of single-trial, auditory P300 and contingent negative variation (CNV) evoked potentials (EPs) were derived using independent component analysis (ICA) and cluster analysis. The method was tested in simulation including a study of the electric dipole equivalents of the s...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/28/8/001
更新日期:2007-08-01 00:00:00
abstract::The heart rate responds dynamically to various intrinsic and environmental stimuli. The autonomic nervous system is said to play a major role in this response. Multifractal analysis offers a novel method to assess the response of cardiac interbeat intervals. Twenty-four hour ECG recordings of RR interbeat intervals (o...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/32/10/014
更新日期:2011-10-01 00:00:00
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journal_title:Physiological measurement
pub_type: 临床试验,杂志文章
doi:10.1088/0967-3334/26/2/003
更新日期:2005-04-01 00:00:00
abstract::In this paper we present the concept, the design and the test procedure for a DSP-based high-precision and high-performance wide-band (up to 10 MHz) bioimpedance analyser module for application in EIT or bioimpedance spectroscopy. The module implements a digital concept with appropriate signal conditioning hardware fo...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/22/1/303
更新日期:2001-02-01 00:00:00
abstract::A new algorithm for classifying ECG recording quality based on the detection of commonly observed ECG contaminants which often render the ECG unusable for diagnostic purposes was evaluated. Contaminants (baseline drift, flat line, QRS-artefact, spurious spikes, amplitude stepwise changes, noise) were detected on indiv...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/33/9/1435
更新日期:2012-09-01 00:00:00
abstract::Wearables such as accelerometers are emerging as powerful tools for quantifying gait in various environments. Flexibility in wearable location may improve ease of use and data acquisition during instrumented testing. However, change of location may impact algorithm functionality when evaluating associated gait charact...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/37/10/1785
更新日期:2016-10-01 00:00:00
abstract:OBJECTIVE:Non-contact physiological measurement is a growing research area that allows capturing vital signs such as heart rate (HR) and breathing rate (BR) comfortably and unobtrusively with remote devices. However, most of the approaches work only in bright environments in which subtle photoplethysmographic and balli...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/1361-6579/aae625
更新日期:2018-10-30 00:00:00
abstract::The transthoracic electrical impedance is an important defibrillation parameter, affecting the defibrillating current amplitude and energy, and therefore the defibrillation efficiency. A close relationship between transthoracic impedance and defibrillation success rate was observed. Pre-shock measurements (using low a...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/21/1/318
更新日期:2000-02-01 00:00:00
abstract::A new method for the measurement of pulmonary gas exchange during inhalational anaesthesia is described which measures fresh gas and exhaust gas flows using carbon dioxide as an extractable marker gas. The theoretical precision of the method was compared by Monte Carlo modelling with other approaches which use marker ...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/25/1/001
更新日期:2004-02-01 00:00:00
abstract::Unstable gait is a risk factor for falls. Wearable accelerometers enable remote monitoring of daily walking. Here, new methods for measuring stride-to-stride oscillations are validated against optical motion capture, normative data determined, and dependency on walking speed investigated. Walks by 13 young people (mea...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/36/5/857
更新日期:2015-05-01 00:00:00
abstract::Tetrapolar linear arrays are commonly used in geophysics to explore the vertical distribution of resistivity values in a geological section of a specific point on the earth. This technique, known as vertical electric sounding (VES), uses different electrode configurations. For our experiments, we utilized its more pop...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/18/1/007
更新日期:1997-02-01 00:00:00
abstract::The outside of the skin of the forearm is typically 15 mV more negative than the inside. Stretching the skin causes a reduction in the magnitude of this skin potential V, which we observe as a motion artifact delta V. We seek to determine the origin of this motion artifact by successively stripping 12 layers of the sk...
journal_title:Physiological measurement
pub_type: 杂志文章
doi:10.1088/0967-3334/17/2/003
更新日期:1996-05-01 00:00:00