Abstract：
BACKGROUND:Pteridine metabolic pathway is unusual features of Leishmania, which is necessary for the growth of parasite. Leishmania has evolved a complex and versatile pteridine salvage network which has the ability of scavenging a wide area of the conjugated and unconjugated pteridines especially folate and biopterin. In this study, we focus on the inhibition of ptr1 gene expression. METHODS:L. major ptr1 gene was cloned into pcDNA3 and digested using KpnI and BamHI. The gene was subcloned so that antisense will transcribe and called pcDNA-rPTR. Leishmania major was cultured and late logarithmic-phase promastigotes were harvested. The promastigotes were divided into two groups. One group was transfected with 50 µg of pcDNA-rPTR, whereas the other group was transfected with pcDNA3. Transfected cells were cultured and plated onto semi-solid media. Mouse pritonean macrophages were transfected using pcDNA-rPTR-tansfected promastigotes. Western blotting was performed on mouse transfected pritonean macrophages and extracts from transfected promastigotes of L. major using a L. major ptr1 antibody raised in rabbits. RESULTS:The PTR1 protein was not expressed in pcDNA-rPTR- tansfected promastigotes and mouse macrophage transfected with pcDNA-rPTR- tansfected promastigotes. CONCLUSION:This approach might be used to study the pteridine salvage pathway in Leishmania or to assess the possibility of using gene expression inhibition in the treatment of leishmaniasis.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Kheirandish F,Bandehpour M,Davoudi N,Mosaffa N,Dawood S,Kazemi B,Haghighi A,Khamesipour A,Masjedi H,Mohebali M,Mahboudi Fsubject
Has Abstractpub_date
2013-04-01 00:00:00pages
190-6issue
2eissn
1735-7020issn
2008-238Xjournal_volume
8pub_type
杂志文章abstract：BACKGROUND:The aim of this study was to determine the Toxoplasma antibodies in pregnant women in Zanjan, by ELISA method. METHODS:Blood samples were taken from 500 pregnant women referred to the health centers of Zanjan City, North West Iran, IgM and IgG titers were primarily evaluated. The collected data were analyze...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:We report a case of human nasal nosocomial myiasis in a 69-year-old rural man with numerous live maggots in nostrils. The patient was admitted in Emergency Ward due to dyspnea. After 72 hours the companion of patient discovered larva. The presence of the third instar larva indicated that the infestation was not more t...
journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Chemotherapy still relies on the use of pentavalent antimonials, amphotericin B, paromomycin, miltefosin, and allopurinol. In this study, we explained about the native plant that grows in different regions of Iran and used as anti-leishmanial in Iran and even many other countries. METHODS:This narrative rev...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2017-07-01 00:00:00
abstract：Background:In Iran, both forms of cutaneous (CL) and visceral leishmaniasis (VL) have been re-ported; so the accurate species identification of the parasite(s) and the analysis of genetic diversity are necessary. Methods:The investigation was conducted from 2014 to 2015 in the northwest and south of Iran, where VL is ...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2018-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2015-07-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2016-07-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2013-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章,评审
doi：
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis. METHODS:This study was performed from 2010-20...
journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-04-01 00:00:00
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2017-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2019-01-01 00:00:00
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journal_title：Iranian journal of parasitology
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doi：
更新日期：2018-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2020-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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更新日期：2014-04-01 00:00:00
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2016-01-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2011-03-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type：
doi：
更新日期：2015-04-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2017-10-01 00:00:00
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journal_title：Iranian journal of parasitology
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更新日期：2014-10-01 00:00:00
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
