Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid films.


:In previous work, we developed novel antibacterial hybrid coatings based on dextran containing dispersed Ag NPs (~5 nm, DEX-Ag) aimed to offer dual protection against two of the most common complications associated with implant surgery, infections and rejection of the implant. However, their blood-material interactions are unknown. In this study, we assess the hemocompatibility and biocompatibility of DEX-Ag using fresh blood and two cell lines of the immune system, monocytes (THP-1 cells) and macrophages (PMA-stimulated THP-1 cells). Glass, polyurethane (PU) and bare dextran (DEX) were used as reference surfaces. PU, DEX and DEX-Ag exhibited non-hemolytic properties. Relative to glass (100%), platelet attachment on PU, DEX and DEX-Ag was 15%, 10% and 34%, respectively. Further, we assessed cell morphology and viability, pro-inflammatory cytokines expression (TNF-α and IL-1β), pro-inflammatory eicosanoid expression (Prostaglandin E2, PGE2) and release of reactive oxygen species (ROS, superoxide and H2O2) following incubation of the cells with the surfaces. The morphology and cell viability of THP-1 cells were not affected by DEX-Ag whereas DEX-Ag minimized spreading of PMA-stimulated THP-1 cells and caused a reduction in cell viability (16% relative to other surfaces). Although DEX-Ag slightly enhanced release of ROS, the expression of pro-inflammatory cytokines remained minimal with similar levels of PGE2, as compared to the other surfaces studied. These results highlight low toxicity of DEX-Ag and hold promise for future applications in vivo.


Toxicol Appl Pharmacol


Coll Ferrer MC,Eckmann UN,Composto RJ,Eckmann DM




Has Abstract


2013-11-01 00:00:00














  • Induction of plasma acetylcholinesterase activity in mice challenged with organophosphorus poisons.

    abstract::The restoration of plasma acetylcholinesterase activity in mice following inhibition by organophosphorus pesticides and nerve agents has been attributed to synthesis of new enzyme. It is generally assumed that activity levels return to normal, are stable and do not exceed the normal level. We have observed over the pa...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Duysen EG,Lockridge O

    更新日期:2011-09-01 00:00:00

  • Single-cell RNA sequencing reveals an altered gene expression pattern as a result of CRISPR/cas9-mediated deletion of Gene 33/Mig6 and chronic exposure to hexavalent chromium in human lung epithelial cells.

    abstract::Gene 33 (Mig6, ERRFI1) is an adaptor protein with multiple cellular functions. We recently reported that depletion of this protein promotes lung epithelial cell transformation induced by hexavalent chromium [Cr(VI)]. However, the early molecular events that mediate this process are not clear. In the present study, we ...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Park S,Zhang X,Li C,Yin C,Li J,Fallon JT,Huang W,Xu D

    更新日期:2017-09-01 00:00:00

  • Activation of 3,4,3',4'-tetrachlorobiphenyl to protein-bound metabolites by rat liver microsomal cytochrome P-448-containing monooxygenase system.

    abstract::The specificity of two major types of cytochrome P-450 of rat liver microsomes induced by phenobarbital (PB) and 3-methylcholanthrene (MC) toward activation of three 14C-labeled tetrachlorobiphenyl (TCB) isomers to protein-bound metabolites was examined by intact microsomal and reconstituted monooxygenase systems. The...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Shimada T,Sawabe Y

    更新日期:1983-09-30 00:00:00

  • Rapid and transient stimulation of intracellular reactive oxygen species by melatonin in normal and tumor leukocytes.

    abstract::Melatonin is a modified tryptophan with potent biological activity, exerted by stimulation of specific plasma membrane (MT1/MT2) receptors, by lower affinity intracellular enzymatic targets (quinone reductase, calmodulin), or through its strong anti-oxidant ability. Scattered studies also report a perplexing pro-oxida...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Radogna F,Paternoster L,De Nicola M,Cerella C,Ammendola S,Bedini A,Tarzia G,Aquilano K,Ciriolo M,Ghibelli L

    更新日期:2009-08-15 00:00:00

  • Arsenic-induced alteration in the expression of genes related to type 2 diabetes mellitus.

    abstract::Chronic exposure to high concentrations of arsenic in drinking water is associated with an increased risk for developing type 2 diabetes. The present revision focuses on the effect of arsenic on tissues that participate directly in glucose homeostasis, integrating the most important published information about the imp...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章,评审


    authors: Díaz-Villaseñor A,Burns AL,Hiriart M,Cebrián ME,Ostrosky-Wegman P

    更新日期:2007-12-01 00:00:00

  • Bone marrow toxicity induced by oral benzo[a]pyrene: protection resides at the level of the intestine and liver.

    abstract::The Ah locus encodes a cytosolic receptor that regulates the induction of certain drug-metabolizing enzymes by polycyclic aromatic hydrocarbons such as benzo[a]pyrene. Some inbred mouse strains such as C57BL/6N have the high-affinity Ah receptor (Ahb/Ahb), others such as DBA/2N, the poor-affinity receptor (Ahd/Ahd). P...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Legraverend C,Harrison DE,Ruscetti FW,Nebert DW

    更新日期:1983-09-30 00:00:00

  • Time-varying conjugation of 7,12-dimethylbenz[a]anthracene metabolites in rainbow trout (Oncorhynchus mykiss).

    abstract::Sexually immature rainbow trout were dosed via gavage with 7,12-[14C]dimethylbenz[a]anthracene (DMBA) in order to study hepatic metabolite formation over time and at single and multiple doses. beta-Glucuronidase and aryl sulfatase hydrolyses of bile extracts and subsequent high-pressure liquid chromatography analysis ...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Schnitz AR,Squibb KS,O'Connor JM

    更新日期:1993-07-01 00:00:00

  • Gypenoside XLIX, a naturally occurring gynosaponin, PPAR-alpha dependently inhibits LPS-induced tissue factor expression and activity in human THP-1 monocytic cells.

    abstract::Tissue factor (TF) is involved not only in the progression of atherosclerosis and other cardiovascular diseases, but is also associated with tumor growth, metastasis, and angiogenesis and hence may be an attractive target for directed cancer therapeutics. Gynostemma pentaphyllum (GP) is widely used in the treatment of...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Huang TH,Tran VH,Roufogalis BD,Li Y

    更新日期:2007-01-01 00:00:00

  • Oxidative stress and hepatic stellate cell activation are key events in arsenic induced liver fibrosis in mice.

    abstract::Arsenic is an environmental toxicant and carcinogen. Exposure to arsenic is associated with development of liver fibrosis and portal hypertension through ill defined mechanisms. We evaluated hepatic fibrogenesis after long term arsenic exposure in a murine model. BALB/c mice were exposed to arsenic by daily gavages of...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Ghatak S,Biswas A,Dhali GK,Chowdhury A,Boyer JL,Santra A

    更新日期:2011-02-15 00:00:00

  • Ligand-conjugated mesoporous silica nanorattles based on enzyme targeted prodrug delivery system for effective lung cancer therapy.

    abstract::Epidermal growth factor receptor antibody (EGFRAb) conjugated silica nanorattles (SNs) were synthesized and used to develop receptor mediated endocytosis for targeted drug delivery strategies for cancer therapy. The present study determined that the rate of internalization of silica nanorattles was found to be high in...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Sundarraj S,Thangam R,Sujitha MV,Vimala K,Kannan S

    更新日期:2014-03-15 00:00:00

  • Acute doxorubicin cardiotoxicity alters cardiac cytochrome P450 expression and arachidonic acid metabolism in rats.

    abstract::Doxorubicin (DOX) is a potent anti-neoplastic antibiotic used to treat a variety of malignancies; however, its use is limited by dose-dependent cardiotoxicity. Moreover, there is a strong correlation between cytochrome P450 (CYP)-mediated arachidonic acid metabolites and the pathogenesis of many cardiovascular disease...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Zordoky BN,Anwar-Mohamed A,Aboutabl ME,El-Kadi AO

    更新日期:2010-01-01 00:00:00

  • Pulmonary neutrophil recruitment and bronchial reactivity in formaldehyde-exposed rats are modulated by mast cells and differentially by neuropeptides and nitric oxide.

    abstract::We have used a pharmacological approach to study the mechanisms underlying the rat lung injury and the airway reactivity changes induced by inhalation of formaldehyde (FA) (1% formalin solution, 90 min once a day, 4 days). The reactivity of isolated tracheae and intrapulmonary bronchi were assessed in dose-response cu...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Lino dos Santos Franco A,Damazo AS,Beraldo de Souza HR,Domingos HV,Oliveira-Filho RM,Oliani SM,Costa SK,Tavares de Lima W

    更新日期:2006-07-01 00:00:00

  • Cyclooxygenase-2 mediates interleukin-6 upregulation by vomitoxin (deoxynivalenol) in vitro and in vivo.

    abstract::Interleukin-6 (IL-6) is a central mediator of immunotoxicity that is associated with exposure to the trichothecene vomitoxin (VT). The purpose of this investigation was to test the hypothesis that the inducible cyclooxygenase-2 (COX-2) and its metabolites contribute to VT-induced IL-6 upregulation. VT at 100 to 250 ng...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Moon Y,Pestka JJ

    更新日期:2003-03-01 00:00:00

  • Risk of carotid atherosclerosis associated with genetic polymorphisms of apolipoprotein E and inflammatory genes among arsenic exposed residents in Taiwan.

    abstract::Arsenic had been reported to be associated with carotid atherosclerosis. However, there were few studies to evaluate the association between the susceptible gene of lipid metabolism and inflammation and carotid atherosclerosis among arsenic exposure residents. The aim of the study was to investigate the associations b...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Hsieh YC,Hsieh FI,Lien LM,Chou YL,Chiou HY,Chen CJ

    更新日期:2008-02-15 00:00:00

  • Selective inhibition of phospholipases by atiprimod, a macrophage targeting antiarthritic compound.

    abstract::Azaspiranes are cationic amphiphilic compounds that are active in a number of models of autoimmune disease and transplantation. Repeated administration of cationic amphiphiles induces phospholipid accumulation in a variety of species. The present study was conducted to explore the mechanism of phospholipid accumulatio...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Handler JA,Badger A,Genell CA,Klinkner AM,Kassis S,Waites CR,Bugelski PJ

    更新日期:1999-08-15 00:00:00

  • Mechanistic review of drug-induced steatohepatitis.

    abstract::Drug-induced steatohepatitis is a rare form of liver injury known to be caused by only a handful of compounds. These compounds stimulate the development of steatohepatitis through their toxicity to hepatocyte mitochondria; inhibition of beta-oxidation, mitochondrial respiration, and/or oxidative phosphorylation. Other...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章,评审


    authors: Schumacher JD,Guo GL

    更新日期:2015-11-15 00:00:00

  • Ginsenoside-Rg1 induces angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a.

    abstract::Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. Ginsenoside-Rg1 (Rg1), one of the most abundant active components of ginseng, has been demonstrated as an angiogenesis-stimulating compound in different models. There is increasing evidence implicating microRNAs (miRNAs), a group of n...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Kwok HH,Chan LS,Poon PY,Yue PY,Wong RN

    更新日期:2015-09-15 00:00:00

  • Microarray analysis of altered gene expression in murine fibroblasts transformed by nickel(II) to nickel(II)-resistant malignant phenotype.

    abstract::B200 cells are Ni(II)-transformed mouse BALB/c-3T3 fibroblasts displaying a malignant phenotype and increased resistance to Ni(II) toxicity. In an attempt to find genes whose expression has been altered by the transformation, the Atlas Mouse Stress/Toxicology cDNA Expression Array (Clontech Laboratories, Inc., Palo Al...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Kowara R,Karaczyn A,Cheng RY,Salnikow K,Kasprzak KS

    更新日期:2005-05-15 00:00:00

  • High fat diet-fed obese rats are highly sensitive to doxorubicin-induced cardiotoxicity.

    abstract::Often, chemotherapy by doxorubicin (Adriamycin) is limited due to life threatening cardiotoxicity in patients during and posttherapy. Recently, we have shown that moderate diet restriction remarkably protects against doxorubicin-induced cardiotoxicity. This cardioprotection is accompanied by decreased cardiac oxidativ...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Mitra MS,Donthamsetty S,White B,Mehendale HM

    更新日期:2008-09-15 00:00:00

  • Lactate dehydrogenase isoenzymes in hamster lung lavage fluid after lung injury.

    abstract::Lactate Dehydrogenase Isoenzymes in Hamster Lung Lavage Fluid after Lung Injury. Beck, B. D., Gerson, B., Feldman, H. A., and Brain, J. D. (1983). Toxicol. Appl. Pharmacol. 71, 59-71. Lactate dehydrogenase (LD) levels and isoenzyme patterns were determined in the cell-free supernatant fractions of lung lavage fluid fr...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Beck BD,Gerson B,Feldman HA,Brain JD

    更新日期:1983-10-01 00:00:00

  • Sex-specific modulation of hepatic covalent DNA modifications (I-compounds) by the cytochrome P450 inducer, pregnenolone-16 alpha-carbonitrile.

    abstract::I-compounds are recently discovered, age-dependent covalent DNA modifications, which are detectable by 32P-postlabeling assay for DNA adducts. The effects of the catatoxic antiglucocorticoid, pregnenolone-16 alpha-carbonitrile (PCN), on hepatic and renal I-compound levels have been studied in male and female Sprague-D...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Moorthy B,van Golen KL,Randerath K

    更新日期:1992-04-01 00:00:00

  • Fullerene C60 exposure elicits an oxidative stress response in embryonic zebrafish.

    abstract::Due to its unique physicochemical and optical properties, C60 has raised interest in commercialization for a variety of products. While several reports have determined this nanomaterial to act as a powerful antioxidant, many other studies have demonstrated a strong oxidative potential through photoactivation. To direc...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Usenko CY,Harper SL,Tanguay RL

    更新日期:2008-05-15 00:00:00

  • Assessment of energetic costs of AhR activation by β-naphthoflavone in rainbow trout (Oncorhynchus mykiss) hepatocytes using metabolic flux analysis.

    abstract::Exposure to environmental contaminants such as activators of the aryl hydrocarbon receptor (AhR) leads to the induction of defense and detoxification mechanisms. While these mechanisms allow organisms to metabolize and excrete at least some of these environmental contaminants, it has been proposed that these mechanism...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Nault R,Abdul-Fattah H,Mironov GG,Berezovski MV,Moon TW

    更新日期:2013-08-15 00:00:00

  • Gentamicin-induced renal metabolic alterations in newborn rat kidney: lack of potentiation by vancomycin.

    abstract::Daily subcutaneous administration of 20 or 100 mg/kg gentamicin for 4 days significantly decreased pyridoxal-5'-phosphate and lysosomal specific phosphatidylinositol-phospholipase C (PI-PLC) in newborn rat kidney. The fall in PI-PLC was associated with an elevation in renal phosphatidylinositol, phosphatidylserine, an...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Kacew S,Hewitt WR,Hook JB

    更新日期:1989-06-01 00:00:00

  • The effect of piperonyl butoxide on hepatic cytochrome P-450-dependent monooxygenase activities in rainbow trout (Salmo gairdneri).

    abstract::Although piperonyl butoxide (PBO) is commonly used both in vivo and in vitro as an inhibitor of cytochrome P-450-dependent monooxygenase (MO) activity in a wide variety of species, the effect of PBO on the hepatic MO of fishes has never been characterized. The MO activity in hepatic microsomes from rainbow trout expos...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Erickson DA,Goodrich MS,Lech JJ

    更新日期:1988-06-15 00:00:00

  • Strain differences in tissue concentrations of mercury in inbred mice treated with mercuric chloride.

    abstract::Tissue concentrations of mercury were determined by cold vapor atomic absorption spectrometry in different inbred mouse strains after continuous treatment with HgCl2 (3 weekly sc injections of 0.5 mg/kg bw) for up to 12 weeks. Except for the thymus, in which steadily increasing mercury concentrations were found, in st...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Griem P,Scholz E,Turfeld M,Zander D,Wiesner U,Dunemann L,Gleichmann E

    更新日期:1997-05-01 00:00:00

  • Speciation of arsenic in biological samples.

    abstract::Speciation of arsenicals in biological samples is an essential tool to gain insight into its distribution in tissues and its species-specific toxicity to target organs. Biological samples (urine, hair, fingernail) examined in the present study were collected from 41 people of West Bengal, India, who were drinking arse...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Mandal BK,Ogra Y,Anzai K,Suzuki KT

    更新日期:2004-08-01 00:00:00

  • Mutagenicity testing of agent orange components and related chemicals.

    abstract::Components of the herbicide Agent Orange--2,4-dichlorophenoxyacetic acid (2,4,-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) and their esters, and the contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)--and related chemicals were tested for mutagenicity using Salmonella typhimurium strains TA98, TA100, TA1535...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Mortelmans K,Haworth S,Speck W,Zeiger E

    更新日期:1984-08-01 00:00:00

  • Two distinct classes of CCAAT box elements that bind nuclear factor-Y/alpha-actinin-4: potential role in human CYP1A1 regulation.

    abstract::A negative regulatory element (NRE1; position -794 to -774) was previously identified that mediates the downregulation of CYP1A1, including partial suppression of Ah receptor-dependent induction. The CCAAT-box binding protein, nuclear factor-Y (NF-Y), is a component of one of two protein complexes that specifically an...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Poch MT,Al-Kassim L,Smolinski SM,Hines RN

    更新日期:2004-09-15 00:00:00

  • Tissue-specific changes in glutathione and cysteine after buthionine sulfoximine treatment of rats and the potential for artifacts in thiol levels resulting from tissue preparation.

    abstract::L-Buthionine-S,R-sulfoximine (BSO), a potent inhibitor of gamma-glutamylcysteine synthetase, is commonly used as an experimental tool for the specific depletion of glutathione. Since cysteine is a key precursor for glutathione biosynthesis, we investigated the possibility that BSO might also affect the free cysteine p...

    journal_title:Toxicology and applied pharmacology

    pub_type: 杂志文章


    authors: Standeven AM,Wetterhahn KE

    更新日期:1991-02-01 00:00:00