Insulin and insulin-like growth factor I binding to cultured rat glomerular mesangial cells.


:The potential effects of insulin and insulin-like growth factor I (IGF-I) on mesangial cell (MC) metabolism and growth were examined. Radiolabeled insulin or IGF-I were incubated with cell membranes from rapidly proliferating (subconfluent) or nonproliferating (confluent) MC in the presence of increasing concentrations of unlabeled heterologous and homologous ligands (0-10(-6) M). Insulin binding to MC was specific and saturable, with Scatchard analysis of binding data showing the characteristic curvilinear plot. The predicted insulin binding maximum of 4.2 X 10(-12) M/100 micrograms protein for a theoretical high affinity site was consistent with a relatively low density of receptors, which were the same in proliferating and nonproliferating cell preparations. Specific binding of IGF-I to MC was also demonstrated. Binding data for membranes from proliferating cultures generated a linear Scatchard plot, which predicted a binding maximum of 3.5-9.7 X 10(-11) M/100 micrograms protein and a Kd of 2.0-3.2 X 10(-9) M. In contrast, membranes from nonproliferating cultures had no demonstrable specific binding of IGF-I. Covalent cross-linking of radiolabeled IGF-I to membranes from subconfluent cells demonstrated specific binding to a 145K membrane protein. A 95K membrane protein from a partially purified receptor preparation demonstrated autophosphorylation when incubated with 5 X 10(-9) M IGF-I. Incubation of MC with 10(-9) M IGF-I doubled cellular growth rates, an effect that could be duplicated only with high concentrations (10(-6) M) of insulin. These observations indicate that MC express predominantly receptors for IGF-I, and that growth stimulatory effects of physiological concentrations of IGF-I and pharmacological concentrations of insulin are probably mediated through the IGF-I receptor.






Abrass CK,Raugi GJ,Gabourel LS,Lovett DH




Has Abstract


1988-11-01 00:00:00












  • Expression of messenger ribonucleic acid encoding tissue inhibitor of metalloproteinases-2 within ovine follicles and corpora lutea.

    abstract::Metalloproteinase inhibitors, such as tissue inhibitors of metalloproteinases 1 and 2 (TIMP-1 and -2), play a key role in the regulation of metalloproteinases that modify extracellular matrix composition. Although expression of TIMP-1 within ovarian tissues has been well characterized, little information is available ...


    pub_type: 杂志文章


    authors: Smith GW,McCrone S,Petersen SL,Smith MF

    更新日期:1995-02-01 00:00:00

  • des-Asp-angiotensin I: its identification in rat blood and confirmation as a substrate for converting enzyme.

    abstract::The observation that angiotensin III is present in the circulation of the rat in amounts similar to those of angiotensin II has led to the notion that it may, in part, be formed by the action of converting enzyme on des-Asp-angiotensin I without the prior formation of angiotensin II. This possibility was studied in co...


    pub_type: 杂志文章


    authors: Garcia Del Rio C,Smellie WS,Morton JJ

    更新日期:1981-02-01 00:00:00

  • Transport of maternal[3H]melatonin to suckling rats and the fate of [3H]melatonin in the neonatal rat.

    abstract::The question of whether maternal melatonin could be transported in milk to suckling rats was investigated because melatonin is probably not produced in these animals during the first 10 days of life. [3H]Melatonin was found to be rapidly transferred from the maternal circulation into lactating mammary tissue, and the ...


    pub_type: 杂志文章


    authors: Reppert SM,Klein DC

    更新日期:1978-02-01 00:00:00

  • Neuroendocrine function in adult female transgenic mice expressing the human growth hormone gene.

    abstract::Adult female transgenic mice expressing the human GH (hGH) gene with mouse metallothionein-I promoter are sterile. To evaluate the hypothalamic-pituitary function in these animals, adult female transgenic mice and nontransgenic normal littermates were ovariectomized. On days 7 and 8 after ovariectomy, mice were inject...


    pub_type: 杂志文章


    authors: Chandrashekar V,Bartke A,Wagner TE

    更新日期:1992-04-01 00:00:00

  • Luteinizing Hormone Facilitates Antral Follicular Maturation and Survival via Thecal Paracrine Signaling in Cattle.

    abstract::LH supplementation in assisted reproductive technology cycles improves the ongoing pregnancy rate in women with poor ovarian response (POR). However, our knowledge of the precise role of LH during the follicular phase of the menstrual cycle is incomplete. To explore the role of LH in the maturation of small antral fol...


    pub_type: 杂志文章


    authors: Hattori K,Orisaka M,Fukuda S,Tajima K,Yamazaki Y,Mizutani T,Yoshida Y

    更新日期:2018-06-01 00:00:00

  • Increased Bone Turnover and Possible Accelerated Fracture Healing in a Murine Model With an Increased Circulating C-Type Natriuretic Peptide.

    abstract::Recent studies have revealed that C-type natriuretic peptide (CNP) is a potent stimulator of endochondral bone growth. Nevertheless, the effect of CNP on bone turnover has not yet been well studied. To elucidate this issue, we investigated the bone phenotype of a mouse model with elevated plasma CNP concentrations (SA...


    pub_type: 杂志文章


    authors: Kondo E,Yasoda A,Fujii T,Nakao K,Yamashita Y,Ueda-Sakane Y,Kanamoto N,Miura M,Arai H,Mukoyama M,Inagaki N,Nakao K

    更新日期:2015-07-01 00:00:00

  • Thyroid hormone economy in pregnant rats near term: a "physiological" animal model of nonthyroidal illness?

    abstract::We have studied the changes in thyroid hormone economy that occur in normal pregnant rats between 17-22 days of gestation. T4 and T3 decreased in all extrathyroidal tissues studied, namely plasma, liver, kidney, lung, heart, and skeletal muscle. The exception is the concentration of T3 in cerebral cortex, which remain...


    pub_type: 杂志文章


    authors: Calvo R,Obregon MJ,Ruiz de Oña C,Ferreiro B,Escobar Del Rey E,Morreale de Escobar G

    更新日期:1990-07-01 00:00:00

  • Effect of ACTH, alpha-MSH, and beta-Lipotropin on calcium and phosphorus metabolism in the rabbit.

    abstract::Studies were undertaken to investigate the effects of synthetic 1-24 adrenocorticotropin (ACTH), bovine alpha melanocyte-stimulating hormone (alpha-MSH), and ovine beta lipotropin (beta-LPH) on plasma calcium and phosphate in rabbits. Equimolar concentrations of these hormones were infused intravenously in intact and ...


    pub_type: 杂志文章


    authors: Gildersleeve DL,Pearson TA,Baghdiantz A,Foster GV

    更新日期:1975-12-01 00:00:00

  • Binding of radioiodinated bovine parathyroid hormone-(1-84) to canine renal cortical membranes.

    abstract::Binding of PTH to purified canine renal cortical membranes was investigated using biologically active radioiodinated bovine PTH-(1-84) [bPTH-(1-84)] as radioligand. PTH-(1-84) is thought to be the major circulating form of bioactive PTH, but oxidative inactivation upon iodination has prevented its use as a radioligand...


    pub_type: 杂志文章


    authors: Rizzoli RE,Murray TM,Marx SJ,Aurbach GD

    更新日期:1983-04-01 00:00:00

  • Rapid bidirectional effects of insulin on hypothalamic noradrenergic and serotoninergic neuronal activity in the rat: role in glucose homeostasis.

    abstract::Glucose release from the liver is mediated by hypothalamic norepinephrine (NE) neuronal activity, but glucose itself (or a metabolite of it) exerts negative feedback effects on central NE activity. The aim of the present study was to investigate a possible role for insulin in these central glucose homeostatic mechanis...


    pub_type: 杂志文章


    authors: Smythe GA,Bradshaw JE,Nicholson MV,Grunstein HS,Storlien LH

    更新日期:1985-10-01 00:00:00

  • TSH regulates pendrin membrane abundance and enhances iodide efflux in thyroid cells.

    abstract::Thyroid hormones are essential for normal development and metabolism. Their synthesis requires transport of iodide into thyroid follicles. The mechanisms involving the apical efflux of iodide into the follicular lumen are poorly elucidated. The discovery of mutations in the SLC26A4 gene in patients with Pendred syndro...


    pub_type: 杂志文章


    authors: Pesce L,Bizhanova A,Caraballo JC,Westphal W,Butti ML,Comellas A,Kopp P

    更新日期:2012-01-01 00:00:00

  • Hormone release by islet B cell-enriched and A and D cell-enriched populations prepared by flow cytometry.

    abstract::Dispersed pancreatic islet cells were analyzed for their low forward angle light scatter using flow cytometry. The cells produced a distinct light scatter pattern which appeared to be a function of cell size and not cell granularity. RIA of hormone content of cells collected from different regions of the pattern revea...


    pub_type: 杂志文章


    authors: Fletcher DJ,Grogan WM,Barras E,Weir GC

    更新日期:1983-11-01 00:00:00

  • Histamine as a neuroendocrine regulator of the stress-induced release of peripheral catecholamines.

    abstract::The involvement of hypothalamic histaminergic neurons in the stress-induced release of peripheral catecholamines was studied in conscious, freely moving male rats. Blood samples were obtained via a catheter in a femoral artery. Intracerebroventricular infusion of histamine (HA; 30 micrograms) increased the plasma conc...


    pub_type: 杂志文章


    authors: Knigge U,Matzen S,Warberg J

    更新日期:1990-03-01 00:00:00

  • Integrin activates receptor-like protein tyrosine phosphatase alpha, Src, and Rho to increase prolactin gene expression through a final phosphatidylinositol 3-kinase/cytoskeletal pathway that is additive with insulin.

    abstract::We previously showed that receptor-like protein tyrosine phosphatase (RPTP)-alpha inhibited insulin-increased prolactin gene transcription. Others suggested that RPTPalpha was a key intermediary between integrins and activation of Src. We present evidence that inhibition of insulin-increased prolactin gene transcripti...


    pub_type: 杂志文章


    authors: Vulin AI,Jacob KK,Stanley FM

    更新日期:2005-08-01 00:00:00

  • Effect of continuous intravenous administration of human metastin 45-54 on the neuroendocrine activity of the hypothalamic-pituitary-testicular axis in the adult male rhesus monkey (Macaca mulatta).

    abstract::In agonadal juvenile male monkeys, continuous administration of human metastin 45-54 (hu metastin 45-54) leads to desensitization of its receptor, G protein-coupled receptor 54 (GPR54), and decreased LH. The present study extended this observation to the adult male monkey, a more preclinically relevant model in which ...


    pub_type: 杂志文章


    authors: Ramaswamy S,Seminara SB,Pohl CR,DiPietro MJ,Crowley WF Jr,Plant TM

    更新日期:2007-07-01 00:00:00

  • The effect of a null mutation in the follicle-stimulating hormone receptor gene on mouse reproduction.

    abstract::To investigate further brain-pituitary-gonadal interrelationships we have generated mice in which the gene encoding the FSH receptor has been disrupted. Female FSH receptor knockout (FSHRKO) mice were infertile. The ovaries were significantly reduced in size, with follicular development arrested at the preantral stage...


    pub_type: 杂志文章


    authors: Abel MH,Wootton AN,Wilkins V,Huhtaniemi I,Knight PG,Charlton HM

    更新日期:2000-05-01 00:00:00

  • The regulation of 24,25-dihydroxyvitamin D3 production in cultures of monkey kidney cells.

    abstract::Primary kidney cell cultures from normal rhesus monkeys were used to study the regulation of 24,25-dihydroxyvitamin D3 production. Kidney cells were grown to confluency in the modified National Cancer Institute Medium NCTC 135 with 2% fetal calf serum and then maintained in a serum-free medium (2% bovine serum albumin...


    pub_type: 杂志文章


    authors: Juan D,DeLUCA HF

    更新日期:1977-10-01 00:00:00

  • Epidermal growth factor elevates intracellular pH in chicken granulosa cells by activating protein kinase C.

    abstract::Previous studies from our laboratory have demonstrated that epidermal growth factor (EGF), induces intracellular alkalinization in chicken granulosa cells by activating a sodium-dependent and amiloride-sensitive Na+/H+ antiporter. In the present investigation we have examined the possible involvement of protein kinase...


    pub_type: 杂志文章


    authors: Li M,Morley P,Tsang BK

    更新日期:1991-12-01 00:00:00

  • Insulinlike Growth Factor 1 Gene Variation in Vertebrates.

    abstract::IGF1-a small, single-chain, secreted peptide in mammals-is essential for normal somatic growth and is involved in a variety of other physiological and pathophysiological processes. IGF1 expression appears to be controlled by several different signaling mechanisms in mammals, with GH playing a key role by activating an...


    pub_type: 杂志文章


    authors: Rotwein P

    更新日期:2018-06-01 00:00:00

  • Scheduled feeding and 24-hour rhythms of N-acetylserotonin and melatonin in rats.

    abstract::Male rats, kept under a lighting condition of 14-h light, 10-h dark, were subjected to scheduled feeding regimens. Food was available either in the early light phase or the early dark phase. The 24-h rhythms of serum corticosterone and of N-acetylserotonin (NAS) and melatonin (MT) in the pineal and serum were determin...


    pub_type: 杂志文章


    authors: Ho AK,Burns TG,Grota LJ,Brown GM

    更新日期:1985-05-01 00:00:00

  • Studies on rat pituitary homografts. I. In vitro biosynthesis and release of growth hormone and prolactin.

    abstract::Homologous anterior pituitaries grafted under the kidney capsule in hypophysectomized rats were studied 30 days after transplantation. Some cells maintained the ultrastructural features peculiar to the various cell types of normotopic glands, while the others were characterized by few, small, dense granules, spherical...


    pub_type: 杂志文章


    authors: Zanini A,Giannattasio G,De Camilli P,Panerai AE,Müller EE,Meldolesi J

    更新日期:1979-01-01 00:00:00

  • Expression cloning of the aldosterone target cell-specific 11 beta-hydroxysteroid dehydrogenase from rabbit collecting duct cells.

    abstract::11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD) is thought to confer aldosterone specificity to mineralocorticoid target cells by protecting the mineralocorticoid receptor from occupancy by endogenous glucocorticoids. We have recently described a novel isoform of 11-OHSD in the renal aldosterone target cells (11 b...


    pub_type: 杂志文章


    authors: Náray-Fejes-Tóth A,Fejes-Tóth G

    更新日期:1995-06-01 00:00:00

  • Nitric oxide modulates the release of vasopressin from rat hypothalamic explants.

    abstract::The enzyme nitric oxide (NO) synthase is present in the paraventricular nucleus, while nitric oxide has recently been shown to inhibit the stimulated release of corticotrophin-releasing hormone (CRH) in vitro. Thus the possible role of NO in regulating, vasopressin (AVP), which also plays an important role in pituitar...


    pub_type: 杂志文章


    authors: Yasin S,Costa A,Trainer P,Windle R,Forsling ML,Grossman A

    更新日期:1993-09-01 00:00:00

  • The effect of lithium on glucose- and tolbutamide-induced insulin release and glucose tolerance in the intact rat.

    abstract::The effects of lithium on stimulus-induced insulin release and glucose tolerance were examined in intact, unanesthetized, and unrestrained rats with indwelling intravascular catheters. In the first study, a 150-mg iv glucose pulse was administered at 30 min after rapid iv injection of lithium carbonate (1 meq/kg) or v...


    pub_type: 杂志文章


    authors: Shah JH,Pishdad G

    更新日期:1980-11-01 00:00:00

  • Osteoprotegerin-deficient male mice as a model for severe alveolar bone loss: comparison with RANKL-overexpressing transgenic male mice.

    abstract::Periodontitis, an inflammatory disease of periodontal tissues, is characterized by excessive alveolar bone resorption. An increase in the receptor activator of nuclear factor-κB ligand (RANKL) to osteoprotegerin (OPG) ratio is thought to reflect the severity of periodontitis. Here, we examined alveolar bone loss in OP...


    pub_type: 杂志文章


    authors: Koide M,Kobayashi Y,Ninomiya T,Nakamura M,Yasuda H,Arai Y,Okahashi N,Yoshinari N,Takahashi N,Udagawa N

    更新日期:2013-02-01 00:00:00

  • In vitro evidence that an 11-kilodalton N-terminal fragment of proopiomelanocortin is a growth factor specifically stimulating the development of lactotrophs in rat pituitary during postnatal life.

    abstract::We have previously shown that in reaggregate cell cultures of 14-day-old female rat pituitary, LHRH is capable of stimulating lactotrophs to enter the S-phase of the cell cycle, and that this effect is mediated by paracrine growth factors secreted from gonadotrophs. In the present report we describe the isolation, pur...


    pub_type: 杂志文章


    authors: Tilemans D,Andries M,Proost P,Devreese B,Van Beeumen J,Denef C

    更新日期:1994-07-01 00:00:00

  • Regulation of cholecystokinin octapeptide secretion by rat cerebral cortical cells in primary culture.

    abstract::The regulation of cholecystokinin octapeptide (CCK-8) secretion was studied using a fetal cerebral cortical cell culture system. After 2-3 weeks in culture, the cells were utilized for short-term release experiments. CCK-8 was measured by RIA and its identity was confirmed by HPLC. Depolarization of the cells with K+ ...


    pub_type: 杂志文章


    authors: Abucham J,Reichlin S

    更新日期:1991-12-01 00:00:00

  • Production of insulin-like growth factors, platelet-derived growth factor, and transforming growth factors and their role in the density-dependent growth regulation of a differentiated embryonal carcinoma cell line.

    abstract::P19 EPI-7, a differentiated murine embryonal carcinoma cell line with an epithelioid morphology, does not require external growth factors for proliferation under clonal and subconfluent conditions. At saturation density, however, cells become quiescent in the G1/G0 phase of the cell cycle from which they can be restim...


    pub_type: 杂志文章


    authors: van Zoelen EJ,Koornneef I,Holthuis JC,Ward-van Oostwaard TM,Feijen A,de Poorter TL,Mummery CL,van Buul-Offers SC

    更新日期:1989-04-01 00:00:00

  • Cyclic adenosine monophosphate signaling in the primate corpus luteum: maintenance of protein kinase A activity throughout the luteal phase of the menstrual cycle.

    abstract::Recent studies from our laboratory (Endocrinology 136:4762-4768, 1995) demonstrating that the expression of cAMP-dependent nuclear transcription factor CREB (cAMP response element binding protein) is lost following ovulation in macaques has revealed a novel mechanism by which the cytoplasmic and nuclear actions the cA...


    pub_type: 杂志文章


    authors: Benyo DF,Zeleznik AJ

    更新日期:1997-08-01 00:00:00

  • C-terminal fragments of the gastrin-releasing peptide precursor stimulate cell proliferation via a novel receptor.

    abstract::There are many precedents for the production from a single precursor of multiple peptides, with independent receptors and different bioactivities. Gastrin-releasing peptide (GRP) is initially synthesized as amino acids 1-27 of a 125-residue precursor, proGRP, and is subsequently cleaved and amidated to form GRP18-27. ...


    pub_type: 杂志文章


    authors: Patel O,Dumesny C,Shulkes A,Baldwin GS

    更新日期:2007-03-01 00:00:00