In vivo inhibition of hypertrophic scars by implantable ginsenoside-Rg3-loaded electrospun fibrous membranes.

Abstract:

:Clinically, hypertrophic scarring (HS) is a major concern for patients and has been a challenge for surgeons, as there is a lack of treatments that can intervene early in the formation of HS. This study reports on a Chinese drug, 20(R)-ginsenoside Rg3 (GS-Rg3), which can inhibit in vivo the early formation of HS and later HS hyperplasia by inducing the apoptosis of fibroblasts, inhibiting inflammation and down-regulating VEGF expression. Implantable biodegradable GS-Rg3-loaded poly(l-lactide) (PLA) fibrous membranes were successfully fabricated using co-electrospinning technology to control drug release and improve drug utilization. The in vivo releasing time of GS-Rg3 lasts for 3 months, and the drug concentration released in rabbits can be controlled by varying the drug content of the electrospun fibers. Histological observations of HE staining indicate that GS-Rg3/PLA significantly inhibits the HS formation, with obvious improvements in terms of dermis layer thickness, epidermis layer thickness and fibroblast proliferation. The results of immunohistochemistry staining and Masson's trichrome staining demonstrate that GS-Rg3/PLA electrospun fibrous membranes significantly inhibit HS formation, with decreased expression of collagen fibers and microvessels. VEGF protein levels are much lower in the group treated with GS-Rg3/PLA eletrospun membranes compared with other groups. These results demonstrate that GS-Rg3 is a novel drug, capable of inhibiting the early formation of HS and later HS hyperplasia. GS-Rg3/PLA electrospun membrane is a very promising new treatment for early and long-term treatment of HS.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

Cheng L,Sun X,Hu C,Jin R,Sun B,Shi Y,Zhang L,Cui W,Zhang Y

doi

10.1016/j.actbio.2013.07.040

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

9461-73

issue

12

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(13)00388-7

journal_volume

9

pub_type

杂志文章
  • Cancellous bone-like porous Fe@Zn scaffolds with core-shell-structured skeletons for biodegradable bone implants.

    abstract::Three-dimensional (3D) porous zinc (Zn) with a moderate degradation rate is a promising candidate for biodegradable bone scaffolds. However, fabrication of such scaffolds with adequate mechanical properties remains a challenge. Moreover, the composition, crystallography and microstructure of the in vivo degradation pr...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2020.11.032

    authors: He J,Fang J,Wei P,Li Y,Guo H,Mei Q,Ren F

    更新日期:2021-02-01 00:00:00

  • Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for neural tissue engineering.

    abstract::The current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps that will produce results similar to autograft without requiring the harvest of autologous donor tissue. Aligned and random polycaprolactone/gelatin (PCL/gelatin) nanofibrous scaffolds...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.01.039

    authors: Gupta D,Venugopal J,Prabhakaran MP,Dev VR,Low S,Choon AT,Ramakrishna S

    更新日期:2009-09-01 00:00:00

  • Effects of clodronate and alendronate on osteoclast and osteoblast co-cultures on silk-hydroxyapatite films.

    abstract::The goal of this study was to explore the effects of osteoporosis-related therapeutics on bone remodeling in vitro. A previously established bone-tissue mimetic system consisting of silk protein biomaterials in combination with hydroxyapatite and human cells was used for the study. Silk-hydroxyapatite films were pre-c...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.09.028

    authors: Hayden RS,Vollrath M,Kaplan DL

    更新日期:2014-01-01 00:00:00

  • Phosphonium-based ionic liquids as modifiers for biomedical grade poly(vinyl chloride).

    abstract::This work reports and discusses the influence of four phosphonium-based ionic liquids (PhILs), namely trihexyl(tetradecyl) phosphonium dicyanamide, [P(6,6,6,14)][dca]; trihexyl(tetradecyl) phosphonium bis(trifluoromethylsulfonyl)imide, [P(6,6,6,14)][Tf(2)N]; tetrabutyl phosphonium bromide, [P(4,4,4,4)][Br]; and tetrab...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.10.034

    authors: Dias AM,Marceneiro S,Braga ME,Coelho JF,Ferreira AG,Simões PN,Veiga HI,Tomé LC,Marrucho IM,Esperança JM,Matias AA,Duarte CM,Rebelo LP,de Sousa HC

    更新日期:2012-03-01 00:00:00

  • Effect of diffusion coating of Nd on the corrosion resistance of biodegradable Mg implants in simulated physiological electrolyte.

    abstract::The effect of diffusion coating of Nd on the corrosion performance of Mg-1.2%Nd-0.5%Y-0.5%Zr-0.4%Ca alloy (EW10X04) used as a new structural material for biodegradable implants was evaluated in a simulated physiological electrolyte. The initial Nd layer with a thickness of 1 μm was obtained by a physical vapor deposit...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.01.009

    authors: Levy G,Aghion E

    更新日期:2013-11-01 00:00:00

  • Regulation of proteoglycan production by varying glucose concentrations controls fiber formation in tissue engineered menisci.

    abstract::Fibrillar collagens are highly prevalent in the extracellular matrix of all connective tissues and therefore commonly used as a biomaterial in tissue engineering applications. In the native environment, collagen fibers are arranged in a complex hierarchical structure that is often difficult to recreate in a tissue eng...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.09.026

    authors: McCorry MC,Kim J,Springer NL,Sandy J,Plaas A,Bonassar LJ

    更新日期:2019-12-01 00:00:00

  • Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials.

    abstract::The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular cell compatibilit...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.08.001

    authors: McKenna KA,Hinds MT,Sarao RC,Wu PC,Maslen CL,Glanville RW,Babcock D,Gregory KW

    更新日期:2012-01-01 00:00:00

  • Influence of nanostructural environment and fluid flow on osteoblast-like cell behavior: a model for cell-mechanics studies.

    abstract::Introducing nanoroughness on various biomaterials has been shown to profoundly effect cell-material interactions. Similarly, physical forces act on a diverse array of cells and tissues. Particularly in bone, the tissue experiences compressive or tensile forces resulting in fluid shear stress. The current study aimed t...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.02.011

    authors: Prodanov L,Semeins CM,van Loon JJ,te Riet J,Jansen JA,Klein-Nulend J,Walboomers XF

    更新日期:2013-05-01 00:00:00

  • Effect of cleaning and sterilization on titanium implant surface properties and cellular response.

    abstract::Titanium (Ti) has been widely used as an implant material due to the excellent biocompatibility and corrosion resistance of its oxide surface. Biomaterials must be sterile before implantation, but the effects of sterilization on their surface properties have been less well studied. The effects of cleaning and steriliz...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.11.026

    authors: Park JH,Olivares-Navarrete R,Baier RE,Meyer AE,Tannenbaum R,Boyan BD,Schwartz Z

    更新日期:2012-05-01 00:00:00

  • Decoding rejuvenating effects of mechanical loading on skeletal aging using in vivo μCT imaging and deep learning.

    abstract::Throughout the process of aging, dynamic changes of bone material, micro- and macro-architecture result in a loss of strength and therefore in an increased likelihood of fragility fractures. To date, precise contributions of age-related changes in bone (re)modeling and (de)mineralization dynamics to this fragility inc...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2020.02.007

    authors: Asgharzadeh P,Röhrle O,Willie BM,Birkhold AI

    更新日期:2020-04-01 00:00:00

  • Functional role of glycosaminoglycans in decellularized lung extracellular matrix.

    abstract::Despite progress in use of decellularized lung scaffolds in ex vivo lung bioengineering schemes, including use of gels and other materials derived from the scaffolds, the detailed composition and functional role of extracellular matrix (ECM) proteoglycans (PGs) and their glycosaminoglycan (GAG) chains remaining in dec...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.11.029

    authors: Uhl FE,Zhang F,Pouliot RA,Uriarte JJ,Rolandsson Enes S,Han X,Ouyang Y,Xia K,Westergren-Thorsson G,Malmström A,Hallgren O,Linhardt RJ,Weiss DJ

    更新日期:2020-01-15 00:00:00

  • Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices--implications in the aging of resin-dentin bonds.

    abstract::Biomineralization is a dehydration process in which water from the intrafibrillar compartments of collagen fibrils are progressively replaced by apatites. As water is an important element that induces a lack of durability of resin-dentin bonds, this study has examined the use of a biomimetic remineralization strategy ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.03.021

    authors: Kim YK,Mai S,Mazzoni A,Liu Y,Tezvergil-Mutluay A,Takahashi K,Zhang K,Pashley DH,Tay FR

    更新日期:2010-09-01 00:00:00

  • Constitutive modeling of the stress-strain behavior of F-actin filament networks.

    abstract::The central role of the cytoskeleton in both healthy and diseased cellular functions makes it a compelling subject for detailed three-dimensional (3D) micromechanical modeling. Microstructural features of the cytoskeleton govern the cell's mechanical behavior in many of the regulating cellular functions including cell...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2007.12.007

    authors: Palmer JS,Boyce MC

    更新日期:2008-05-01 00:00:00

  • A novel hydrogel-collagen composite improves functionality of an injectable extracellular matrix.

    abstract::Cellular transplantation is now closer to becoming a practical clinical strategy to repair, regenerate or restore the function of skin, muscle, nerves and pancreatic islets. In this study we sought to develop a simple injectable collagen matrix that would preserve the normal cellular organization of skin cells. Three ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.04.024

    authors: Hartwell R,Leung V,Chavez-Munoz C,Nabai L,Yang H,Ko F,Ghahary A

    更新日期:2011-08-01 00:00:00

  • A strain-mediated corrosion model for bioabsorbable metallic stents.

    abstract::This paper presents a strain-mediated phenomenological corrosion model, based on the discrete finite element modelling method which was developed for use with the ANSYS Implicit finite element code. The corrosion model was calibrated from experimental data and used to simulate the corrosion performance of a WE43 magne...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.04.020

    authors: Galvin E,O'Brien D,Cummins C,Mac Donald BJ,Lally C

    更新日期:2017-06-01 00:00:00

  • 72-Hour in vivo evaluation of nitric oxide generating artificial lung gas exchange fibers in sheep.

    abstract::The large, densely packed artificial surface area of artificial lungs results in rapid clotting and device failure. Surface generated nitric oxide (NO) can be used to reduce platelet activation and coagulation on gas exchange fibers, while not inducing patient bleeding due to its short half-life in blood. To generate ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.04.004

    authors: Lai A,Demarest CT,Do-Nguyen CC,Ukita R,Skoog DJ,Carleton NM,Amoako KA,Montoya PJ,Cook KE

    更新日期:2019-05-01 00:00:00

  • Research on an Mg-Zn alloy as a degradable biomaterial.

    abstract::In this study a binary Mg-Zn magnesium alloy was researched as a degradable biomedical material. An Mg-Zn alloy fabricated with high-purity raw materials and using a clean melting process had very low levels of impurities. After solid solution treatment and hot working the grain size of the Mg-Zn alloy was finer and a...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.06.028

    authors: Zhang S,Zhang X,Zhao C,Li J,Song Y,Xie C,Tao H,Zhang Y,He Y,Jiang Y,Bian Y

    更新日期:2010-02-01 00:00:00

  • Corrosion fatigue of biomedical metallic alloys: mechanisms and mitigation.

    abstract::Cyclic stresses are often related to the premature mechanical failure of metallic biomaterials. The complex interaction between fatigue and corrosion in the physiological environment has been subject of many investigations. In this context, microstructure, heat treatments, plastic deformation, surface finishing and co...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章,评审

    doi:10.1016/j.actbio.2011.09.012

    authors: Antunes RA,de Oliveira MC

    更新日期:2012-03-01 00:00:00

  • Efficient tuning of siRNA dose response by combining mixed polymer nanocarriers with simple kinetic modeling.

    abstract::Two of the most prominent challenges that limit the clinical success of siRNA therapies are a lack of control over cargo release from the delivery vehicle and an incomplete understanding of the link between gene silencing dynamics and siRNA dosing. Herein, we address these challenges through the formulation of siRNA p...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.01.003

    authors: Greco CT,Muir VG,Epps TH 3rd,Sullivan MO

    更新日期:2017-03-01 00:00:00

  • Cell-laden hydrogel/titanium microhybrids: Site-specific cell delivery to metallic implants for improved integration.

    abstract:UNLABELLED:Porous titanium implants are widely used in dental, orthopaedic and otorhinolaryngology fields to improve implant integration to host tissue. A possible step further to improve the integration with the host is the incorporation of autologous cells in porous titanium structures via cell-laden hydrogels. Fast ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.01.023

    authors: Koenig G,Ozcelik H,Haesler L,Cihova M,Ciftci S,Dupret-Bories A,Debry C,Stelzle M,Lavalle P,Vrana NE

    更新日期:2016-03-01 00:00:00

  • The processing of ultrafine-grained Mg tubes for biodegradable stents.

    abstract::An investigation was carried out on equal-channel angular pressing (ECAP) and extrusion processing of a ZM21 Mg alloy to obtain an improved candidate material for the manufacturing of biodegradable Mg stents. Ultrafine-grain size billets of the ZM21 alloy were obtained by two-stage ECAP aimed at achieving an initial r...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.01.010

    authors: Ge Q,Dellasega D,Demir AG,Vedani M

    更新日期:2013-11-01 00:00:00

  • Peptide-mediated shape- and size-tunable synthesis of gold nanostructures.

    abstract::While several biological processes have been shown to be useful for the production of well-designed, inorganic nanostructures, the mechanism(s) controlling the size and shape of nano and micron size particles remains elusive. Here we report on the controlled size- and shape-specific production of gold nanostructures u...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.01.019

    authors: Kim J,Rheem Y,Yoo B,Chong Y,Bozhilov KN,Kim D,Sadowsky MJ,Hur HG,Myung NV

    更新日期:2010-07-01 00:00:00

  • Absorption performance of iodixanol-imprinted polymers in aqueous and blood plasma media.

    abstract::This paper presents the preparation and absorption performance of iodixanol-imprinted polymers in aqueous and blood plasma media in vitro for biomedical applications. The imprinted polymers were prepared by non-covalent imprinting of iodixanol in a matrix of poly(4-vinylpyridine) crosslinked by ethylene glycol dimetha...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.11.007

    authors: Liu Z,Bucknall DG,Allen MG

    更新日期:2010-06-01 00:00:00

  • Bioengineered surfaces to improve the blood compatibility of biomaterials through direct thrombin inactivation.

    abstract::Thrombus formation, due to thrombin generation, is a major problem affecting blood-contacting medical devices. This work aimed to develop a new strategy to improve the hemocompatibility of such devices by the immobilization of a naturally occurring thrombin inhibitor into a nanostructured surface. Boophilin, a direct ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.07.020

    authors: Freitas SC,Cereija TB,Figueiredo AC,Osório H,Pereira PJ,Barbosa MA,Martins MC

    更新日期:2012-11-01 00:00:00

  • Substrate stiffness orchestrates epithelial cellular heterogeneity with controlled proliferative pattern via E-cadherin/β-catenin mechanotransduction.

    abstract:UNLABELLED:Epithelial cellular heterogeneity has been observed in pathological tissues with abnormal matrix stiffness and cells cultured on rigid substrates. However, it remains unclear how matrix stiffness influences cellular heterogeneity formation in multi-cellular population. Here, we demonstrated that cellular het...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.05.025

    authors: Wang B,Qin P,Zhao H,Xia T,Wang J,Liu L,Zhu L,Xu J,Huang C,Shi Y,Du Y

    更新日期:2016-09-01 00:00:00

  • In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects.

    abstract::The easy clinical handling and applicability of biomaterials has become a focus of materials research due to rapidly increasing time and cost pressures in the public health sector. The present study assesses the in vitro and in vivo performance of a flexible, mouldable, cottonwool-like nanocomposite based on poly(lact...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2008.11.030

    authors: Schneider OD,Weber F,Brunner TJ,Loher S,Ehrbar M,Schmidlin PR,Stark WJ

    更新日期:2009-06-01 00:00:00

  • Synthesis, characterization and osteoblastic activity of polycaprolactone nanofibers coated with biomimetic calcium phosphate.

    abstract::Immersion of electrospun polycaprolactone (PCL) nanofiber mats in calcium phosphate solutions similar to simulated body fluid resulted in deposition of biomimetic calcium phosphate layer on the nanofibers and thus a highly bioactive novel scaffold has been developed for bone tissue engineering. Coatings with adequate ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.04.037

    authors: Mavis B,Demirtaş TT,Gümüşderelioğlu M,Gündüz G,Colak U

    更新日期:2009-10-01 00:00:00

  • Comparison of PEG chain length and density on amphiphilic macromolecular nanocarriers: self-assembled and unimolecular micelles.

    abstract::Two classes of amphiphilic macromolecules were evaluated for drug delivery applications: those that exist as unimolecular micelles and those that self-assemble in aqueous solution to form micelles. This study compares the poly(ethylene glycol) (PEG) chain length and density that constitute the corona of both classes. ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2008.10.019

    authors: Wang J,del Rosario LS,Demirdirek B,Bae A,Uhrich KE

    更新日期:2009-03-01 00:00:00

  • Early osteogenic signal expression of rat bone marrow stromal cells is influenced by both hydroxyapatite nanoparticle content and initial cell seeding density in biodegradable nanocomposite scaffolds.

    abstract::Incorporation of hydroxyapatite (HA) within a degradable polymeric scaffold may provide a favorable synthetic microenvironment that more closely mimics natural bone tissue physiology. Both incorporation of HA nanoparticles and alterations of the paracrine cell-cell signaling distance may affect the intercellular signa...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.11.007

    authors: Kim K,Dean D,Lu A,Mikos AG,Fisher JP

    更新日期:2011-03-01 00:00:00

  • In vivo degradation of low temperature calcium and magnesium phosphate ceramics in a heterotopic model.

    abstract::Bone replacement using synthetic and degradable materials is desirable in various clinical conditions. Most applied commercial materials are based on hydroxyapatite, which is not chemically degradable under physiological conditions. Here we report the effect of a long-term intramuscular implantation regime on the diss...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.05.022

    authors: Klammert U,Ignatius A,Wolfram U,Reuther T,Gbureck U

    更新日期:2011-09-01 00:00:00