Use of ELISA to reveal rodent infections in plague surveillance and control programmes.

Abstract:

:To assess its potential applications in plague surveillance and control programmes, an enzyme-linked immunosorbent assay (ELISA) that captures the specific Yersinia pestis F1 antigen was used to determine the amounts of this antigen present in samples of blood and spleens from laboratory rats with acute plague, in the buboes of rats with chronic plague, and in tissues from the carcasses of rats that had died from plague. In rats with acute plague, bacteria and F1 antigen were detected in samples of blood 2-4 days after the rats had been subcutaneously inoculated with virulent Y. pestis. Although F1 antigen was not detected in blood samples at the onset of bacteraemia, average antigen levels of 10,240 ng/ml for blood clots and 4829 ng/ml for sera were detected during severe bacteraemia. Immediately after the rats died from acute plague, antigen levels in their spleens of up to 410 micrograms/g of tissue were detected. For rats with chronic plague, ELISA antigen levels of 1600 ng/g to 51 micrograms/g were detected in buboes that contained viable plague bacilli. In samples of spleen, liver, kidney and heart from rat carcasses, F1 antigen was detected up to 20 days after death. In contrast, it was possible to isolate Y. pestis bacilli from these samples only up to 6 days after death.

authors

Williams JE

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

341-5

issue

3

eissn

0042-9686

issn

1564-0604

journal_volume

68

pub_type

杂志文章
  • Epidemiology of endemic viral hepatitis in an urban area of India: a retrospective community study in Alwar.

    abstract::In a community study during a reference period of 1 year, 192 cases of jaundice were detected in an urban population of 69,440 in Alwar, Rajasthan. Detected by paramedics and confirmed by physicians, these cases gave an annual incidence of 2.76 (95% CI: 2.37-3.15) per 1000 population. At least one of these patients di...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Singh J,Prakash C,Gupta RS,Bora D,Jain DC,Datta KK

    更新日期:1997-01-01 00:00:00

  • Interventions for the control of diarrhoeal diseases among young children: measles immunization.

    abstract::The effects of measles immunization on diarrhea morbidity and mortality are reviewed using data from field studies and theoretical calculations. 2 types of measles-associated diarrhea are distinguished: with-measles diarrhea, which starts between 1 week prerash-onset and 4 weeks postrash-onset, and postmeasles diarr...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Feachem RG,Koblinsky MA

    更新日期:1983-01-01 00:00:00

  • The virulence in the guinea-pig of isoniazid-sensitive tubercle bacilli isolated from South Indian patients before treatment and after three months of chemotherapy.

    abstract::In order to find out whether chemotherapy with isoniazid affects the virulence in the guinea-pig of tubercle bacilli that do not develop resistance to the drug, virulence tests were carried out on isoniazid-sensitive cultures obtained from 20 South Indian tuberculous patients before treatment and after three months of...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: SUBBAIAH TV,BHATIA AL,GERAGHTY E,MITCHISON DA,RADHAKRISHNA S,SELKON JB

    更新日期:1961-01-01 00:00:00

  • Detection of HIV-1 antibodies in blood specimens spotted on filter-paper.

    abstract::Described are the results of an international collaborative study to evaluate the use of whole blood samples spotted on filter-paper (BSP) for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1). BSP samples were collected from 40 patients at risk for HIV-1 infection and tested blindly using com...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Lillo F,Varnier OE,Mantia E,Terragna A,van der Groen G,Van Kerckhoven I,Mortimer PP,Parry JV,Bayliss G,Tamashiro H

    更新日期:1992-01-01 00:00:00

  • ASSESSMENT OF THE POTENCY OF TUBERCULIN IN HUMANS AND GUINEA-PIGS.

    abstract::The biological effects of tuberculin in an organism infected with tubercle bacilli are due to an unknown number of different substances, which are present in varying concentrations in different tuberculin preparations. Consequently, the response to tuberculin tests with various products is likely to vary with the biol...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: HANSEN OG,LINDQVIST K,WAALER H

    更新日期:1964-01-01 00:00:00

  • Present trends and the future in rabies research.

    abstract::Until very recently, rabies research had made few notable advances since the time of Pasteur, but during the past few years the use of modern virological techniques has led to rapid progress in research on the rabies virus. This article summarizes the present state of knowledge of pathogenesis, immunology, cultivation...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Campbell JB,Kaplan MM,Koprowski H,Kuwert E,Sokol F,Wiktor TJ

    更新日期:1968-01-01 00:00:00

  • Sex- and age- specific relations between economic development, economic inequality and homicide rates in people aged 0-24 years: a cross-sectional analysis.

    abstract:OBJECTIVE:To test whether relations between economic development, economic inequality, and child and youth homicide rates are sex- and age-specific, and whether a country's wealth modifies the impact of economic inequality on homicide rates. METHODS:Outcome variables were homicide rates around 1994 in males and female...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Butchart A,Engström K

    更新日期:2002-01-01 00:00:00

  • A study on the behaviour of leprosy in serological tests for syphilis.

    abstract::Blood samples from 58 leprosy patients were tested by the MK1R and VDRL slide tests, the latter being performed on normally inactivated sera and on the same sera heated for 5 minutes at 65 degrees C. The MK1R test showed fewer false positive reactions than the VDRL test on normally inactivated sera. The VDRL test on t...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: NARULA RN,GUPTA VP

    更新日期:1953-01-01 00:00:00

  • Observations on the physiology of adult anophelines in Asia.

    abstract::Some recent physiological observations made in Afghanistan, Ceylon and Burma on adults of Anopheles culicifacies and A. aconitus are described. The specimens from Ceylon and Burma, in particular, showed a peculiar physiological process in which, at the beginning of the dry season, the coagulated, dark red, and almost ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Büttiker W

    更新日期:1958-01-01 00:00:00

  • Striving to provide universal health coverage in Kazakhstan.

    abstract::Four decades after the declaration of Alma Ata, Kazakhstan still struggles to provide basic health care to its citizens. This may now be changing. Andrey Shukshin reports. ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 新闻

    doi:10.2471/BLT.19.020419

    authors:

    更新日期:2019-04-01 00:00:00

  • Effect of a control programme on transmission of Schistosoma mansoni on an irrigated estate in Tanzania.

    abstract::Three methods were used to measure the level of transmission of infections of Schistosoma mansoni on an irrigated sugar estate in northern Tanzania. The studies were carried out over a period of 3 years, during a programme for the control of the host snail Biomphalaria pfeifferi. During the second and third years a ma...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Fenwick A

    更新日期:1972-01-01 00:00:00

  • Natural Brucella infection in Argentine wild foxes.

    abstract::In the course of an investigation in 1962-64 into the natural occurrence of brucellosis among grey foxes in Argentina, agglutination tests were performed on 728 sera of the foxes Dusicyon gymnocercus antiquus and D. griseus griseus, captured in the provinces of Buenos Aires and Rio Negro. Agglutination titres of from ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Szyfres B,González Tomé J

    更新日期:1966-01-01 00:00:00

  • ELISA in the diagnosis of Wuchereria bancrofti infection in man: a technique for reducing cross-reactivity.

    abstract::The cross-reactivity of filarial heteroantigens, the non-availability of adult Wuchereria bancrofti for antigen extraction, and the lack of structural information on purified filarial heteroantigens make the serological diagnosis of W. bancrofti infection in man a difficult problem. The use of immune sera from cattle ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Dissanayake S,Ismail MM

    更新日期:1980-01-01 00:00:00

  • Poliomyelitis in 1954.

    abstract::As in an earlier study, the authors have endeavoured to review the world incidence of poliomyelitis on the basis of the available statistical information (official notifications of cases and deaths) and to compare these data with data received from various sources regarding the prevailing viruses.It is certainly not y...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: FREYCHE MJ,PAYNE AM

    更新日期:1956-01-01 00:00:00

  • Determination of physiological age in anophelines and of age distribution in anopheline populations in the USSR.

    abstract::A technique is described whereby it is possible to make an accurate determination of the physiological age of anopheline females, i.e., the number of gonotrophic cycles through which the female has passed. This technique is based on counts of the dilatations left in the ovarioles subsequent to each maturation of folli...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: BEKLEMISHEV WN,DETINOVA TS,POLOVODOVA VP

    更新日期:1959-01-01 00:00:00

  • Field application to malaria studies of the passive haemagglutination (PHA) test with lyophilized cells.

    abstract::Lyophilized cells for the passive (formerly indirect) haemagglutination test for malaria were tested in a small field trial. A comparison was made with results obtained with samples from the same persons by another method in another laboratory (Center for Disease Control, Atlanta, GA, USA). The use of lyophilized cell...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Brögger RC,Mathews HM

    更新日期:1975-01-01 00:00:00

  • The efficacy of live influenza vaccines.

    abstract::Administration of live influenza vaccine produces an asymptomatic immunizing infection of the respiratory tract with the appearance of antibodies in the secretions of the respiratory passages.Because of natural variation in the antigenic and biological properties of the influenza virus, new vaccines must be developed ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Smorodincev AA

    更新日期:1969-01-01 00:00:00

  • Antibiotic resistance of nasopharyngeal isolates of Streptococcus pneumoniae from children in Lesotho.

    abstract::Villages associated with the Lesotho Highlands Development Agency were randomized with a bias in favour of larger villages, and children < 5 years of age from cluster-randomized households in these villages were chosen for the assessment of antibiotic resistance in pneumococci. Children of the same age group attending...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Mthwalo M,Wasas A,Huebner R,Koornhof HJ,Klugman KP

    更新日期:1998-01-01 00:00:00

  • Viral hepatitis in Israel: the effect of canvassing physicians on notifications and the apparent epidemiological pattern.

    abstract::Notifications of morbidity are frequently incomplete but they represent the only practicable way of carrying out large-scale surveillance. Studies of hepatitis in the Central District of Israel offered an opportunity of assessing the reliability of routine data. The regular canvassing of physicians resulted in a 2-3-f...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Brachott D,Mosley JW

    更新日期:1972-01-01 00:00:00

  • Laboratory colonization of Toxorhynchites brevipalpis.

    abstract::Toxorhynchites brevipalpis, a predator on larvae of Aedes aegypti and other mosquitos, was successfully colonized in the laboratory. At 25 degrees C, embryonic development was completed within 50 hours of oviposition, while larval and pupal development together took a further 27-41 days. The adult mosquitos mated in c...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Trpis M,Gerberg EJ

    更新日期:1973-05-01 00:00:00

  • Making a public-private partnership work--an insider's view. Interview by John Maurice.

    abstract::In November 1999, Dr Amadou Diarra was appointed senior director of Bristol-Myers Squibb's Secure the Future Initiative. Launched 18 months ago, this public-private partnership is seeking, together with the governments of nine African countries, innovative ways of reducing the transmission and the impact of HIV/AIDS, ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 面试

    doi:

    authors: Diarra A

    更新日期:2001-01-01 00:00:00

  • Monographs for medicines on WHO's Model List of Essential Medicines.

    abstract:Objective:To raise awareness about the importance of public pharmaceutical standards, identify if and, if so, where current pharmacopeias are falling short in the development of new and complete monographs and foster collaboration among the various pharmacopeias, to prioritize, develop and make available standards for ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.17.205807

    authors: Roth L,Adler M,Jain T,Bempong D

    更新日期:2018-06-01 00:00:00

  • Reference toxins for antivenin standardization.

    abstract::Potency determinations in mice of venom samples from snakes of the species Bothrops jararaca, which had been caught in an area of less than 10 000 km(2), gave the following principal results:(1) Various methods of drying-i.e., closed and continuous vacuum-drying at 5 degrees and 37 degrees C and freeze-drying (lyophil...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: SCHOTTLER WH

    更新日期:1958-01-01 00:00:00

  • Further studies on BCG vaccine prepared by tween 80 method with particular reference to effect of freeze-drying.

    abstract::The immunizing potency of liquid BCG vaccine prepared by the Tween 80 method was compared with that of control vaccine prepared by the usual grinding method. The former vaccine revealed higher immunizing properties than the latter although its inoculation dose was only one-fifth as great.With sodium glutamate as adjuv...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: TAKANO K,OBAYASHI Y,NOGUCHI T

    更新日期:1957-01-01 00:00:00

  • A 5-year study of patients with pulmonary tuberculosis in a concurrent comparison of home and sanatorium treatment for one year with isoniazid plus PAS.

    abstract::This report from the Tuberculosis Chemotherapy Centre, Madras, summarizes the progress over a 5-year period of 193 patients with newly diagnosed, sputum-positive pulmonary tuberculosis who were admitted to a concurrent comparison of home and sanatorium treatment for one year with isoniazid plus PAS. Previous reports h...

    journal_title:Bulletin of the World Health Organization

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Dawson JJ,Devadatta S,Fox W,Radhakrishna S,Ramakrishnan CV,Somasundaram PR,Stott H,Tripathy SP,Velu S

    更新日期:1966-01-01 00:00:00

  • Single haemolysis for the assay of antibodies to some haemagglutinating arboviruses.

    abstract::The single radial haemolysis reaction has been shown to be suitable for the quantitative assay of specific antibody to West Nile and Sindbis viruses. Only 5 mul of undiluted serum are needed for the test, which can be performed on crude preparations of antigen and without removal of nonspecific inhibitors. It is there...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Duca M,Duca E,Ionescu L,Abdalla H

    更新日期:1979-01-01 00:00:00

  • The present status of control of Culex pipiens fatigans.

    abstract::At present, larvicidal attack is the only feasible measure for the control of Culex pipiens fatigans. However, in the cities of the tropics, which are the principal sites of the filariasis problem, this has usually been a losing battle. The proper method of larval control is to eliminate the breeding sites; instead th...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Brown AW

    更新日期:1967-01-01 00:00:00

  • Mortality from neonatal tetanus in Indonesia: results of two surveys.

    abstract::Two, 30-cluster, retrospective surveys of deaths from neonatal tetanus in Indonesia were conducted during 1982. The first survey, in the city of Jakarta, identified 16 deaths from neonatal tetanus among 2310 live births, giving a mortality rate of 6.9 per 1000 live births. The second survey covered 19 of Indonesia's 2...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Arnold RB,Soewarso TI,Karyadi A

    更新日期:1986-01-01 00:00:00

  • Prevalence of anti-Rift-Valley-fever IgM antibody in abattoir workers in the Nile delta during the 1993 outbreak in Egypt.

    abstract::In the early summer of 1993, an outbreak of Rift Valley fever (RVF) was reported among both humans and animals in Aswan Governorate, Upper Egypt. To determine whether RVF infection had spread to the Nile delta region of the country, we carried out a cross-sectional survey of 1181 occupationally exposed abattoir worker...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Abu-Elyazeed R,el-Sharkawy S,Olson J,Botros B,Soliman A,Salib A,Cummings C,Arthur R

    更新日期:1996-01-01 00:00:00

  • Experimental investigations into the therapeutic effect of griseogulvin in favus caused by Trichophyton schoenleinii.

    abstract::Experimental investigations have been carried out in 32 children on the therapeutic effect of griseofulvin in favus caused by Trichophyton schoenleinii and to determine the optimal treatment schedule.It was found that, while daily doses of 3.12 mg, 6.25 mg or 12.50 mg griseofulvin per kg of body-weight did bring about...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Grin EI,Nadazdin M

    更新日期:1965-01-01 00:00:00