Abstract:
:TERT is the main functional unit of telomerase, which maintains telomere length and chromosome structure stability. TERT has been shown to act as a key factor in various biological processes, such as cell proliferation, via uncharacterized mechanisms. We transfected HEp-2 laryngeal carcinoma cells with a TERT overexpressing adenovirus (Ad-TERT) and TERT shRNA silencing adenovirus (Ad-sh-TERT), and examined the effect on TERT and the AP-1 transcription factor subunits c-Fos and c-Jun using RT-PCR and western blot analysis. TERT mRNA expression was quantified using RT-PCR in 24 human laryngeal carcinoma samples, and TERT protein co-expression with AP-1 was investigated in a human laryngeal carcinoma tissue microarray using quantum-dot based immunofluorescence. The effect of specific ERK and p38 inhibitors on ERK, p38, c-Jun and c-Fos phosphorylation was investigated in TERT-overexpressing HEp-2 cells. TERT overexpression led to increased TERT, c-Jun and c-Fos mRNA and protein expression and increased cell proliferation, while TERT silencing had the opposite effects. TERT mRNA expression levels were positively correlated with c-Fos and c-Jun mRNA in human laryngeal carcinoma tissue. TERT and AP-1 protein were expressed at high levels and positively correlated in laryngeal carcinoma tissues. Treatment of TERT-overexpressing HEp-2 cells with specific p38 and ERK inhibitors indicated that TERT modulates the expression and phosphorylation of the AP-1 subunits c-Jun and c-Fos through the p38 and ERK signaling pathways. In conclusion, the results of this study indicate that TERT is capable of promoting cell proliferation via activation of the AP-1 subunits, c-Jun and c-Fos, in laryngeal carcinoma cells.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Jiang Y,Chen C,Chen SM,Wang YQ,Xu Y,Wang Y,Chen Z,Xiao BK,Tao ZZdoi
10.3892/ol.2013.1344subject
Has Abstractpub_date
2013-07-01 00:00:00pages
75-80issue
1eissn
1792-1074issn
1792-1082pii
ol-06-01-0075journal_volume
6pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Heterogeneous radiological responses (HRRs) among tumor lesions are usually observed following chemotherapy or radiation treatment in cancer patients. When HRR is observed after chemotherapy or radiation treatment, a change in anticancer treatment is recommended due to the clinically high suspicion of resistance in th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3709
更新日期:2015-11-01 00:00:00
abstract::Few reports have explored laparoscopic adnexal tumorectomy as a treatment for large and symptomatic ovarian leiomyomas during pregnancy. The current study presents the case of a patient with a large and symptomatic ovarian leiomyoma at 14 weeks of pregnancy. A laparoscopic adnexal tumorectomy was performed without com...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2596
更新日期:2014-12-01 00:00:00
abstract::Inactivating mutations of the multiple endocrine neoplasia 1 (MEN1) gene cause MEN1 syndrome, characterized by primary hyperparathyroidism (pHPT), and parathyroid and gastro-entero-pancreatic pituitary tumors. At present, only 14 cases of malignant parathyroid tumor have been associated with the syndrome, with 6 cases...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6162
更新日期:2017-07-01 00:00:00
abstract::Silent information regulator 6 (SIRT6) is broadly considered as a tumor suppressor due to its function in the suppression of oncogene expression. However, the role of SIRT6 in colorectal cancer stem cells (CSCs) remains uncharacterized. In the present study, it was demonstrated that SIRT6 expression was reduced in col...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7989
更新日期:2018-04-01 00:00:00
abstract::Mammary fibromatosis is a rare and locally aggressive benign tumor of the breast; it originates from fibroblasts and myofibroblasts within the breast parenchyma and does not metastasize. The condition is locally aggressive and has a high rate of recurrence. The etiology of mammary fibromatosis is unknown. Breast imagi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6337
更新日期:2017-08-01 00:00:00
abstract::Homoisoflavanone-1 is a natural compound that may be extracted from the Chinese medicinal herb Polygonatum odoratum, which has pronounced antioxidant activities. The present study reports that homoisoflavanone-1 significantly inhibited tumor cell growth and induced apoptosis in A549 non-small cell lung cancer (NSCLC) ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9085
更新日期:2018-09-01 00:00:00
abstract::A standard therapeutic approach for advanced malignant thymoma has yet to be defined given the rarity of this condition. We present a patient with advanced thymoma, evaluated as inoperable at diagnosis due to multiple serosal metastases. The strong constitution and determination of the patient allowed treatment with s...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.282
更新日期:2011-05-01 00:00:00
abstract::The concentrations required for curcumin to exert its anticancer activity (IC50, 20 µM) are difficult to achieve in the blood plasma of patients, due to the low bioavailability of the compound. Therefore, much effort has been devoted to the development of curcumin analogues that exhibit stronger anticancer activity an...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4536
更新日期:2016-06-01 00:00:00
abstract::Gastrinoma is a gastrin-secreting tumor that is associated with Zollinger-Ellison syndrome. The majority of cases occur in the pancreas, followed by the duodenum. Early diagnosis is difficult due to the relative rarity of the tumor and the lack of specific symptoms. In the current study, a 68-year-old female patient p...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4397
更新日期:2016-05-01 00:00:00
abstract::In the present study, the anti-tumor effects of combination treatment with an siRNA targeting B-Raf proto-oncogene serine/threonine kinase (BRAF)V600E and phosphoinositide 3-kinase (PI3K) signaling pathway inhibitors was investigated in melanoma cell lines harboring BRAFV600E. Human melanoma A375 and WM115 cells were ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8614
更新日期:2018-07-01 00:00:00
abstract::The monolocular cystic formation associated with gallbladder carcinoma is an extremely rare condition. A 79-year-old female suffering from upper abdominal pain and distention was admitted to our hospital. Ultrasonography and computed tomography revealed a monolocular cyst with an irregular wall thickness of 15 cm in d...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2010.182
更新日期:2010-11-01 00:00:00
abstract::Human papillomavirus (HPV) is widely known as a cause of cervical intraepithelial neoplasia (CIN) and cervical cancer. The mechanisms involved have been studied by numerous studies. The integration of the virus genome into the host cells results in the abnormal regulation of cell cycle control. HPV can also induce imm...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3295
更新日期:2015-08-01 00:00:00
abstract::In order to assess the metastatic potential of residual hepatocellular carcinoma (HCC) following insufficient radiofrequency ablation (RFA) and to improve the current animal model, an insufficient RFA orthotopic nude mouse model of HCC was developed in the present study. A human HCC orthotopic nude mouse model was est...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10552
更新日期:2019-09-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is generally considered one of the most common gastrointestinal malignant tumors, characterized by high invasiveness and metastatic rate, as well as insidious onset. A relationship between carcinogenicity and aberrant microRNA-139-5p (miR-139-5p) expression has been identified in multipl...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11031
更新日期:2019-12-01 00:00:00
abstract::Adenocarcinoma is one of the most serious diseases that threaten human health. Numerous studies have investigated adenocarcinoma and have obtained a considerable amount of data regarding genes and microRNA (miRNA) in adenocarcinoma. However, studies have only focused on one or a small number of genes and miRNAs, and t...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3676
更新日期:2015-11-01 00:00:00
abstract::The involvement of the female genital tract in transitional cell carcinoma (TCC) has not been fully elucidated in women, although involvement is usually associated with a poor prognosis. The vagina, in particular, is considered to be the most commonly affected gynecological organ, with an incidence of 4% of total TCC ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4894
更新日期:2016-09-01 00:00:00
abstract::Gallbladder cancer (GBC) is a rare but highly aggressive cancer for which no well-accepted prognostic biomarkers have been identified. Thymus cell antigen 1 (Thy1), also known as cluster of differentiation (CD)90, and integrin α6 (ITGA6), also known as CD49f, are important molecules in cancer and putative markers of v...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5341
更新日期:2016-12-01 00:00:00
abstract::Gliomas are the most common type of primary brain tumor in adults, where more than half of the cases are malignant, and the prognosis is poor. The early viral 1A (E1A) protein has been widely recognized to be essential for adenoviral replication and production of progeny virions in human cells, a process that is regul...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7684
更新日期:2018-03-01 00:00:00
abstract::The aim of the present study was to evaluate the utility of diffusion-weighted magnetic resonance imaging (DWI) in the diagnosis of common renal tumors. Conventional magnetic resonance imaging and DWI were performed on 85 patients with renal lesions (54 renal carcinoma and 31 renal angiomyolipoma cases). The apparent ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3605
更新日期:2015-10-01 00:00:00
abstract::In the present study, the incidence of surgical wound complications in patients with large and deep high-grade soft tissue sarcoma (STS) was investigated. In addition, the disease-specific and event-free survival rates were examined in these patients. In total, 30 adult patients with primary high-grade deep STS with a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3289
更新日期:2015-08-01 00:00:00
abstract::Recommendations concerning the intake of vitamin D and/or sunlight exposure in the handling of patients with vitamin D deficiency remain a matter of debate. The present study of the German network of dermato-oncologists (Onkoderm e.V.) refers to an inquiry conducted among general practitioners on this and related issu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.939
更新日期:2012-12-01 00:00:00
abstract::Askin tumors are rare malignant neoplasms that are localized in the thoracopulmonary region and mainly occur in children and adolescents. Further investigation with regard to the effective treatment of this disease is required, since the disease has a low incidence and limited knowledge is available on the biological ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1488
更新日期:2013-10-01 00:00:00
abstract::The Na+/K+-ATPase α subunit is highly expressed in malignant cells. Ouabain, a cardioactive glycoside, binds to the Na+/K+-ATPase α subunit and inhibits the activity of Na+/K+-ATPase. In the present study, the effect of ouabain on the migration of A549 cells was analyzed using the wound healing and transwell chamber m...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1406
更新日期:2013-08-01 00:00:00
abstract::The major aim of the present study was to develop an integrin receptor-targeted liposomal paclitaxel (PTX) to enhance the targeting specificity and therapeutic effect of PTX on hepatocellular carcinoma (HCC) cells. The specific Arg-Gly-Asp (RGD) ligand was conjugated to 1,2-distearoylphosphatidylethanolamine-polyethyl...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3242
更新日期:2015-07-01 00:00:00
abstract::The aim of the present study was to investigate whether oridonin is able to increase the effects of lentinan (LNT) in HepG2 human hepatoblastoma cells by MTT, flow cytometry, reverse transcription-quantitative polymerase chain reaction and western blot analysis. The in vitro results demonstrated that 20 µg/ml of orido...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7485
更新日期:2018-02-01 00:00:00
abstract::Metaplastic carcinoma of the breast (MBC) is a rare and heterogeneous type of neoplasm. Knowledge about its clinical characteristics, prognostic significance and optimal treatment modalities is fragmentary and controversial. The present retrospective study aimed to investigate the prognostic value of the clinicopathol...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6399
更新日期:2017-08-01 00:00:00
abstract::Our previous study demonstrated that the 2-benzyloxybenzaldehyde analog CCY-1a-E2 is a potent compound against HL-60 human leukemia cell lines. To investigate the potential therapeutic application of CCY-1a-E2 for leukemia, we analyzed the antileukemic effects and safety of CCY-1a-E2 in the BALB/c mouse WEHI-3 allogra...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.1079
更新日期:2013-03-01 00:00:00
abstract::In colorectal cancer (CRC), KRAS and BRAF mutations in primary tumors are associated with resistance to anti-epidermal growth factor receptor (anti-EGFR)-based therapies. However, the correlation between KRAS/BRAF mutation in primary tumors and serum has not been well studied. To evaluate the degree of concordance of ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.963
更新日期:2013-01-01 00:00:00
abstract::Lin28 is involved in the progression of several types of tumors. Data collected from clinical trials have suggested that Lin28 expression is correlated with poor prognosis in thyroid carcinoma. The present study was conducted to investigate the association between Lin28 expression and the clinicopathological parameter...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.12358
更新日期:2021-02-01 00:00:00
abstract::Gastric cancer is a pathological process of an accumulation of multigene and multistage mutations. A new gene segment, MDSCBC11, has been previously obtained using a gene chip and is negatively associated with gastric cancer. The present study aimed to clone the full cDNA sequence of the MDSCBC11 segment and to detect...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1595
更新日期:2013-12-01 00:00:00