Unusual cryptosporidiosis cases in Swedish patients: extended molecular characterization of Cryptosporidium viatorum and Cryptosporidium chipmunk genotype I.


:Most human cases of cryptosporidiosis are caused by Cryptosporidium parvum or Cryptosporidium hominis, but the use of molecular diagnostic methods has revealed that several other less common species or genotypes can also be involved. Here, we describe two unusual causes of cryptosporidiosis, one being the recently described species Cryptosporidium viatorum and the other Cryptosporidium chipmunk genotype I. Two Swedish patients who were infected with C. viatorum had travelled to Kenya and Guatemala, respectively, and two others had been infected with Cryptosporidium chipmunk genotype I in Sweden. None of these four patients were immunocompromised, and all four showed classical symptoms of cryptosporidiosis. We performed extensive molecular characterization, including analysis of four loci. The two C. viatorum isolates were found to differ slightly at the 70-kDa heat shock protein locus, which may indicate a local geographical variation in this species that has previously been described exclusively on the Indian subcontinent.






Lebbad M,Beser J,Insulander M,Karlsson L,Mattsson JG,Svenungsson B,Axén C




Has Abstract


2013-12-01 00:00:00














  • Effect of acute phase proteins, especially alpha 2-macroglobulin, on granuloma formation around Schistosoma mansoni eggs in the rat.

    abstract::A new model for the study of granuloma formation in the liver is described. Rats received an injection of 20,000 Schistosoma mansoni eggs in the portal vein and granuloma formation was evaluated at 3, 5 and 7 weeks post-injection. Liver collagen was estimated at the same time and serum procollagen III peptide, a marke...


    pub_type: 杂志文章


    authors: Van Gool J,Van Tiel D,Doenhoff MJ,Van Vugt H

    更新日期:1991-02-01 00:00:00

  • Visualization of Toxoplasma gondii stage conversion by expression of stage-specific dual fluorescent proteins.

    abstract::To recognize the stage conversion of Toxoplasma gondii between tachyzoite and bradyzoite in live host cells, a transgenic T. gondii line, which expressed stage-specific red and green fluorescence, was constructed. T. gondii PLK strain tachyzoites were stably transformed with genes encoding red fluorescent protein (DsR...


    pub_type: 杂志文章


    authors: Unno A,Suzuki K,Batanova T,Cha SY,Jang HK,Kitoh K,Takashima Y

    更新日期:2009-05-01 00:00:00

  • Integrating the landscape epidemiology and genetics of RNA viruses: rabies in domestic dogs as a model.

    abstract::Landscape epidemiology and landscape genetics combine advances in molecular techniques, spatial analyses and epidemiological models to generate a more real-world understanding of infectious disease dynamics and provide powerful new tools for the study of RNA viruses. Using dog rabies as a model we have identified how ...


    pub_type: 杂志文章,评审


    authors: Brunker K,Hampson K,Horton DL,Biek R

    更新日期:2012-12-01 00:00:00

  • Purification of a 64 kDa antigen from Trypanosoma evansi that exhibits cross-reactivity with Trypanosoma vivax.

    abstract::Trypanosoma evansi and Trypanosoma vivax are the most extensively distributed trypanosomes responsible for diseases in livestock. Western blot and indirect immunofluorescence assays revealed a high immunological cross-reaction between these two parasites. An antigen with an apparent molecular mass of 64 kDa (p64), whi...


    pub_type: 杂志文章


    authors: Uzcanga G,Mendoza M,Aso PM,Bubis J

    更新日期:2002-03-01 00:00:00

  • The effect of drugs on the cell cycle of Giardia intestinalis.

    abstract::Flow cytometric analysis of the binucleated protozoan parasite Giardia intestinalis gave DNA histograms with a broad G1 peak and a definable G2 + M peak with twice the DNA content of G1. Twenty-four hour treatment with metronidazole arrested cell cycle progression of susceptible trophozoites in the G2 + M phase, but h...


    pub_type: 杂志文章


    authors: Hoyne GF,Boreham PF,Parsons PG,Ward C,Biggs B

    更新日期:1989-12-01 00:00:00

  • Eimeria tenella in chickens: development of resistance to quinolone anticoccidial drugs.

    abstract::The development of drug resistance by the present Houghton strain of Eimeria tenella to the quinolones, methyl benzoquate and buquinolate, was found to take place after a single experimental passage. The development of resistance was independent of drug selection pressure and showed cross resistance to other quinolone...


    pub_type: 杂志文章


    authors: Chapman HD

    更新日期:1975-08-01 00:00:00

  • Criteria for a proof of migration routes of immature parasites inside hosts exemplified by studies of Strongyloides ratti in the rat.

    abstract::The first rigorous proof applicable to the migration pathway of an infective juvenile macroparasite inside its host is presented. Third-stage larvae of a homogonic strain of Strongyloides ratti applied in exact doses of less than 20 to the skin of the flank of young rats were recovered 16-40 h later in the naso-fronta...


    pub_type: 杂志文章


    authors: Tindall NR,Wilson PA

    更新日期:1988-06-01 00:00:00

  • Costs of resistance in insect-parasite and insect-parasitoid interactions.

    abstract::Most, if not all, organisms face attack by natural enemies and will be selected to evolve some form of defence. Resistance may have costs as well as its obvious benefits. These costs may be associated with actual defence or with the maintenance of the defensive machinery irrespective of whether a challenge occurs. In ...


    pub_type: 杂志文章,评审


    authors: Kraaijeveld AR,Ferrari J,Godfray HC

    更新日期:2002-01-01 00:00:00

  • Cytosolic glutathione S-transferases of Oesophagostomum dentatum.

    abstract::Oesophagostomum dentatum stages were investigated for glutathione S-transferase (GST) expression at the protein and mRNA levels. GST activity was detected in all stages (infectious and parasitic stages including third- and fourth-stage larvae of different ages as well as males and females) and could be dose-dependentl...


    pub_type: 杂志文章


    authors: Joachim A,Ruttkowski B

    更新日期:2008-09-01 00:00:00

  • The effect of non-immune stresses on the development of Strongyloides ratti.

    abstract::Strongyloides ratti is a parasitic nematode of rats. The host immune response against S. ratti affects the development of its free-living generation, favouring the development of free-living adult males and females at the expense of directly developing, infective 3rd-stage larvae. However, how the host immune response...


    pub_type: 杂志文章


    authors: Crook M,Viney ME

    更新日期:2005-09-01 00:00:00

  • Development of a practical immunochromatographic test with recombinant P50 for the diagnosis of Babesia gibsoni infection in dogs.

    abstract::An immunochromatographic test (ICT), using recombinant truncated P50 (P50t), for the detection of antibodies to Babesia gibsoni was developed and evaluated. Whereas all sera from specific pathogen-free dogs were clearly negative, all sera from dogs experimentally infected with B. gibsoni were clearly positive in the I...


    pub_type: 杂志文章


    authors: Verdida RA,Xuan X,Fukumoto S,Huang X,Zhou J,Igarashi I,Claveria FG,Nagasawa H

    更新日期:2005-12-01 00:00:00

  • Hymenolepis nana: the fine structure of the embryonic envelopes.

    abstract::The fine structure of the envelopes surrounding hatched and unhatched oncospheres of Hymenolepis nana has been investigated by transmission and scanning electron microscopy (SEM), together with light microscope histochemical observations of JB-4 embedded material. The oncosphere is surrounded by 3 layers--the capsule,...


    pub_type: 杂志文章


    authors: Fairweather I,Threadgold LT

    更新日期:1981-06-01 00:00:00

  • Age, acquired immunity and the risk of visceral leishmaniasis: a prospective study in Iran.

    abstract::In order to investigate the phenomenon of age-related immunity to visceral leishmaniasis, a 1 year prospective survey was carried out on 5671 people in a Leishmania infantum focus in north-west Iran. The average incidence rate of infection since 1985 was 2.8%/year with all ages equally at risk. One in 13 infections in...


    pub_type: 杂志文章


    authors: Davies CR,Mazloumi Gavgani AS

    更新日期:1999-09-01 00:00:00

  • Trypanosoma cruzi heparin-binding proteins present a flagellar membrane localization and serine proteinase activity.

    abstract::Heparin-binding proteins (HBPs) play a key role in Trypanosoma cruzi-host cell interactions. HBPs recognize heparan sulfate (HS) at the host cell surface and are able to induce the cytoadherence and invasion of this parasite. Herein, we analysed the biochemical properties of the HBPs and also evaluated the expression ...


    pub_type: 杂志文章


    authors: Oliveira FO Jr,Alves CR,Silva FS,Côrtes LM,Toma L,Bouças RI,Aguilar T,Nader HB,Pereira MC

    更新日期:2013-02-01 00:00:00

  • Overlapping antigenic repertoires of variant antigens expressed on the surface of erythrocytes infected by Plasmodium falciparum.

    abstract::Antibodies against variable antigens expressed on the surface of Plasmodium falciparum-infected erythrocytes are believed to be important for protection against malaria. A target for these antibodies is the P. falciparum erythrocyte membrane protein 1, PfEMP1, which is encoded by around 50 var genes and undergoes clon...


    pub_type: 杂志文章


    authors: Giha HA,Staalsoe T,Dodoo D,Elhassan IM,Roper C,Satti GM,Arnot DE,Hviid L,Theander TG

    更新日期:1999-07-01 00:00:00

  • Triphasic locomotor response of a plant-parasitic nematode to avermectin: inhibition by the GABA antagonists bicuculline and picrotoxin.

    abstract::The response of the plant parasitic nematode, Meloidogyne incognita (J2 stage) to avermectin B2a-23-one is triphasic, comprising an initial loss of locomotor activity where the juveniles remain sensitive to touch, a recovery phase and a final loss of activity where the juveniles are relatively insensitive to touch. In...


    pub_type: 杂志文章


    authors: Wright DJ,Birtle AJ,Roberts IT

    更新日期:1984-04-01 00:00:00

  • Low levels of transforming growth factor-beta (TGF-beta) and reduced suppression of Th2-mediated inflammation in hyperreactive human onchocerciasis.

    abstract::Th2-biased inflammation with eosinophilia and IgE production is a hallmark of helminth infections. It is pronounced in hyperreactive onchocerciasis patients ('sowda' or 'local form'), who efficiently kill microfilariae resulting in severe dermatitis and lymphadenitis. In contrast, hyporeactive patients ('generalised f...


    pub_type: 杂志文章


    authors: Korten S,Hoerauf A,Kaifi JT,Büttner DW

    更新日期:2011-01-01 00:00:00

  • Oral oocyst-induced mouse model of toxoplasmosis: effect of infection with Toxoplasma gondii strains of different genotypes, dose, and mouse strains (transgenic, out-bred, in-bred) on pathogenesis and mortality.

    abstract::Humans and other hosts acquire Toxoplasma gondii infection by ingesting tissue cysts in undercooked meat, or by food or drink contaminated with oocysts. Currently, there is no vaccine to prevent clinical disease due this parasite in humans, although, various T. gondii vaccine candidates are being developed. Mice are g...


    pub_type: 杂志文章


    authors: Dubey JP,Ferreira LR,Martins J,McLeod R

    更新日期:2012-01-01 00:00:00

  • Increased levels of thiols protect antimony unresponsive Leishmania donovani field isolates against reactive oxygen species generated by trivalent antimony.

    abstract::The current trend of antimony (Sb) unresponsiveness in the Indian subcontinent is a major impediment to effective chemotherapy of visceral leishmaniasis (VL). Although contributory mechanisms studied in laboratory-raised Sb-R parasites include an up-regulation of drug efflux pumps and increased thiols, their role in c...


    pub_type: 杂志文章


    authors: Mandal G,Wyllie S,Singh N,Sundar S,Fairlamb AH,Chatterjee M

    更新日期:2007-11-01 00:00:00

  • Characterization of Theileria parva which infects waterbuck (Kobus defassa).

    abstract::Theileria-free waterbuck (Kobus defassa) born in captivity were successfully infected with Theileria parva sporozoites derived from ticks infected by feeding on African buffalo (Syncerus caffer). All waterbuck underwent mild infections with the development of sporadic schizont and piroplasm parasitosis when inoculated...


    pub_type: 杂志文章


    authors: Stagg DA,Bishop RP,Morzaria SP,Shaw MK,Wesonga D,Orinda GO,Grootenhuis JG,Molyneux DH,Young AS

    更新日期:1994-06-01 00:00:00

  • Purification and characterization of a 47 kDa protease from Schistosoma mansoni cercarial secretion.

    abstract::Fractionation of Schistosoma mansoni cercariae gland secretion on a Sephadex G-150 column followed by a Superose-12 column in an FPLC system, isolated a 47 kDa protease which migrated as a single band on SDS-PAGE gels. A monoclonal antibody (MAb) was produced which recognizes only the 47 kDa protease, and an immuno-af...


    pub_type: 杂志文章


    authors: Chavez-Olortegui C,Resende M,Tavares CA

    更新日期:1992-10-01 00:00:00

  • A panel of antigens of muscle larvae of Trichinella spiralis and T. pseudospiralis as revealed by two-dimensional western blot and immunoelectron microscopy.

    abstract::This study characterized antigens of Trichinella spiralis and T. pseudospiralis muscle larvae recognized by mice infected with the worms. Two-dimensional (2-D) Western blot analysis revealed some profile of antigenic peptides including: (1) molecular weight (MW); (2) isoelectric points (pI), (3) reactivity to well-def...


    pub_type: 杂志文章


    authors: Wu Z,Nagano I,Takahashi Y

    更新日期:1999-06-01 00:00:00

  • Differentially expressed parasite genes involved in host recognition and invasion of the triactinomyxon stage of Myxobolus cerebralis (Myxozoa).

    abstract::The host recognition and invasion process of Myxobolus cerebralis actinospores (triactinomyxon, TAM) was studied on a genetic level. A small-scale in vitro assay was developed to activate a large number of TAMs simultaneously, and to monitor the host invasion in the absence of live fish. The transcriptomes of non-acti...


    pub_type: 杂志文章


    authors: Eszterbauer E,Kallert DM,Grabner D,El-Matbouli M

    更新日期:2009-04-01 00:00:00

  • Pentastomids and the tetrapod lung.

    abstract::Pentastomids comprise a highly specialized taxon of arthropod-like parasites that probably became adapted to the lungs of amphibians and reptiles early in their long evolutionary history. Few other macroparasites exploit this particular niche. Pentastomids are often large, long-lived and yet they cause little observab...


    pub_type: 杂志文章,评审


    authors: Riley J,Henderson RJ

    更新日期:1999-01-01 00:00:00

  • The capacity of the fungus Duddingtonia flagrans to prevent strongyle infections in foals on pasture.

    abstract::A field trial was conducted to evaluate the potential of the nematode-destroying fungus Duddingtonia flagrans to control free-living stages of horse strongyles. In late Spring 2 groups of horses (yearlings) with mixed infections of strongyles were allowed to contaminate 2 equal-sized pastures. One of the groups (F) re...


    pub_type: 杂志文章


    authors: Larsen M,Nansen P,Grøndahl C,Thamsborg SM,Grønvold J,Wolstrup J,Henriksen SA,Monrad J

    更新日期:1996-07-01 00:00:00

  • In vitro titration of Theileria parva tick derived stabilates.

    abstract::Immunization against the protozoan Theileria parva by infection and treatment has proved to be very efficient for the control of East Coast fever, an acute and often-fatal lymphoproliferative tick-borne disease of cattle in Eastern, Central and Southern Africa. The immunizing dose of live T. parva sporozoites used in ...


    pub_type: 杂志文章


    authors: Marcotty T,Speybroeck N,Berkvens D,Chaka G,Besa R,Madder M,Dolan T,Losson B,Brandt J

    更新日期:2004-02-01 00:00:00

  • The effect of dietary sainfoin ( Onobrychis viciifolia) on local cellular responses to Trichostrongylus colubriformis in sheep.

    abstract::The effect of sainfoin (Onobrychis viciifolia) hay consumption on the pathophysiology and local cellular responses of growing lambs during infection with Trichostrongylus colubriformis was investigated. Thirty-two lambs, 16 weeks of age, were allocated to 1 of 4 treatment groups (n=8) that were offered either grass (G...


    pub_type: 杂志文章


    authors: Ríos-de Alvarez L,Greer AW,Jackson F,Athanasiadou S,Kyriazakis I,Huntley JF

    更新日期:2008-08-01 00:00:00

  • Dynamics of Sept4 expression in fibrotic livers of mice infected with Schistosoma japonicum.

    abstract::n order to investigate the dynamics of Septin4 (Sept4) expression and its function in the formation of fibrotic livers in mice infected with Schistosoma japonicum, we constructed the mouse model of S. japonicum egg-induced liver fibrosis for 24 weeks. Immunohistochemical staining, qRT-PCR and Western blot were used to...


    pub_type: 杂志文章


    authors: Duan YN,Qian HY,Qin YW,Zhu DD,He XX,Zhou Q,Yang YN,Bao J,Feng JR,Sun W,Chen JL

    更新日期:2011-07-01 00:00:00

  • Ammonia-induced cellular and immunological changes in juvenile Cyprinus carpio infected with the blood fluke Sanguinicola inermis.

    abstract::Immunological and structural changes in the thymus and pronephros of Cyprinus carpio infected with the blood fluke, Sanguinicola inermis for 30 days, and exposed to 0.5 mg NH4+/1 for 48 and 168 h were investigated. Ultrastructural observations revealed cell disruption and highly vacuolated cytoplasm in the thymus. Of ...


    pub_type: 杂志文章


    authors: Schuwerack PM,Lewis JW,Hoole D,Morley NJ

    更新日期:2001-03-01 00:00:00

  • Female mice mate preferentially with non-parasitized males.

    abstract::If parasitic infection is a driving force in female mate choice, then females should preferentially select parasite-free males. The role of infection on female mate choice in mammals was assessed using a 3-chambered apparatus. A female CD-1 mouse was allowed to choose between 2 tethered male mice, 1 uninfected and 1 i...


    pub_type: 杂志文章


    authors: Ehman KD,Scott ME

    更新日期:2002-11-01 00:00:00