Abstract：
:This present study reports that reactive oxygen species (ROS), which mainly consist of hydroxyl (OH) and superoxide (O2(-)) radicals generated by the photocatalysis of TiO2 nanoparticles (TiO2 NPs) in suspension, can be quantitatively measured by the colorimetric probe-assisted spectrometric method without any interference by the TiO2 NPs. The interference effect of the TiO2 NPs with UV-vis spectroscopy was excluded by the selective separation of p-nitrosodimethylaniline (RNO) and tetrazolium salt (XTT) (probe compounds for OH and O2(-), respectively) from the TiO2 NPs in the suspension by centrifugation at a specified ionic strength adjusted with phosphate buffer (KH2PO4/NaOH, PB) solution. This colorimetric probe-assisted spectrometric method was also successfully applied to measure the steady-state production of photogenerated ROS in a TiO2 NPs suspension.
journal_name
Chemospherejournal_title
Chemosphereauthors
Kim C,Park HJ,Cha S,Yoon Jdoi
10.1016/j.chemosphere.2013.07.023subject
Has Abstractpub_date
2013-11-01 00:00:00pages
2011-5issue
9eissn
0045-6535issn
1879-1298pii
S0045-6535(13)01007-2journal_volume
93pub_type
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