Optical purity determination of threo-methylphenidate hydrochloride using a chiral europium nuclear magnetic resonance (NMR) shift reagent.


:A 1H-NMR spectroscopic method for the determination of the optical purity of threo-methylphenidate hydrochloride is presented. Complexation of the free-base form of the substrate with a chiral Eu(III) shift reagent resulted in two distinct enantiomeric ester methyl proton signals of utility for quantitative work. The accuracy of the method was validated by analyzing six synthetic mixtures of various proportions of (+)- and (-)-threo-methylphenidate. In addition to yielding assay results that were in close agreement with the known weights of each enantiomer in the mixture, and a mean recovery of the (-)-threo-enantiomer of better than 99.0%, the proposed method was capable of accurately measuring as little as 2% of one enantiomer in the presence of the other.


Pharm Res


Pharmaceutical research


Hanna GM,Lau-Cam CA




Has Abstract


1990-07-01 00:00:00












  • Aerosol dispersion of respirable particles in narrow size distributions produced by jet-milling and spray-drying techniques.

    abstract:PURPOSE:To examine the effect of particle size and morphology on aerosol dispersion using jet-milled and spray-dried mannitol particles in narrow size distributions within the respirable range. METHODS:Particle size and morphology were examined by laser diffraction and scanning electron microscopy, respectively. Aeros...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Louey MD,Van Oort M,Hickey AJ

    更新日期:2004-07-01 00:00:00

  • A Simple and Improved Active Loading Method to Efficiently Encapsulate Staurosporine into Lipid-Based Nanoparticles for Enhanced Therapy of Multidrug Resistant Cancer.

    abstract:PURPOSE:This study was aimed at developing a new active loading method to stably encapsulate staurosporine (STS), a water insoluble drug, into lipid-based nanoparticles (LNPs) for drug targeting to tumors. METHODS:A limited amount of DMSO was included during the active loading process to prevent precipitation and faci...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Tang WL,Chen WC,Roy A,Undzys E,Li SD

    更新日期:2016-05-01 00:00:00

  • Spray-Drying, Solvent-Casting and Freeze-Drying Techniques: a Comparative Study on their Suitability for the Enhancement of Drug Dissolution Rates.

    abstract:PURPOSE:Solid dispersions (SDs) represent the most common formulation technique used to increase the dissolution rate of a drug. In this work, the three most common methods used to prepare SDs, namely spray-drying, solvent-casting and freeze-drying, have been compared in order to investigate their effect on increasing ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: De Mohac LM,Caruana R,Cavallaro G,Giammona G,Licciardi M

    更新日期:2020-02-19 00:00:00

  • The preparation and evaluation of a tablet dosage form of cyclosporine in dogs.

    abstract::Cyclosporine (CsA) is commercially available for oral administration as a solution in olive oil with alcohol and an emulsifier. To improve its variable absorption and low patient acceptability, several oral formulations were prepared and tested in vitro and in vivo in dogs. A tablet formulation prepared by direct comp...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Abdallah HY,Mayersohn M

    更新日期:1991-04-01 00:00:00

  • Uptake of colchicine, a microtubular system disrupting agent, by isolated rat hepatocytes.

    abstract::The possible mechanism of hepatic uptake of colchicine (CLC), a microtubule system disrupting agent, was examined using isolated rat hepatocytes. The existence of a carrier-mediated active transport system for CLC was demonstrated. This transport system is saturable, is affected by metabolic inhibitors (dinitrophenol,...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Wierzba K,Sugiyama Y,Okudaira K,Iga T,Hanano M

    更新日期:1989-03-01 00:00:00

  • Intestinal absorption of miltefosine: contribution of passive paracellular transport.

    abstract:PURPOSE:This study aimed to characterize the transepithelial transport of miltefosine (HePC), the first orally effective drug against visceral leishmaniasis, across the intestinal barrier to further understand its oral absorption mechanism. MATERIALS AND METHODS:Caco-2 cell monolayers were used as an in vitro model of...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Ménez C,Buyse M,Dugave C,Farinotti R,Barratt G

    更新日期:2007-03-01 00:00:00

  • Amplification of butyrylcholinesterase and acetylcholinesterase genes in normal and tumor tissues: putative relationship to organophosphorous poisoning.

    abstract::Cholinesterases are ubiquitous carboxylesterase type B enzymes capable of hydrolyzing the neurotransmitter acetylcholine which are transiently expressed in multiple germline, embryonic, and tumor cells. The acute poisoning effects of various organophosphorous compounds are generally attributed to their irreversible co...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章,评审


    authors: Soreq H,Zakut H

    更新日期:1990-01-01 00:00:00

  • A spectroscopic investigation of losartan polymorphs.

    abstract::Losartan, an antihypertensive agent in clinical development, was found to exist in two enantiotropic polymorphic forms, a low-temperature stable form (Form I) and a high-temperature stable form (Form II), the temperatures at which they are stable being related to the transition temperature. X-ray powder diffraction pa...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Raghavan K,Dwivedi A,Campbell GC Jr,Johnston E,Levorse D,McCauley J,Hussain M

    更新日期:1993-06-01 00:00:00

  • Epidermal iontophoresis: I. Development of the ionic mobility-pore model.

    abstract:PURPOSE:An integrated ionic mobility-pore model for epidermal iontophoresis is developed from theoretical considerations using both the free volume and pore restriction forms of the model for a range of solute radii (rj) approaching the pore radii (rp) as well as approximation of the pore restriction form for rj/rp < 0...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Roberts MS,Lai PM,Anissimov YG

    更新日期:1998-10-01 00:00:00

  • Dual Receptor Targeting Cell Penetrating Peptide Modified Liposome for Glioma and Breast Cancer Postoperative Recurrence Therapy.

    abstract:PURPOSE:Cell penetrating peptides (CPPs) were widely used as motifs for drug delivery to tumor. In former study, an RGD reverse sequence dGR was used to develop active-targeting liposome R8dGR-Lip, which showed well penetrating ability and treatment efficiency on glioma model. However, recurrence after tumor resection ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Qiu Y,Yu Q,Liu Y,Tang J,Wang X,Lu Z,Xu Z,He Q

    更新日期:2018-04-26 00:00:00

  • Development of a Two-Dimensional Model for Predicting Transdermal Permeation with the Follicular Pathway: Demonstration with a Caffeine Study.

    abstract:PURPOSE:The development of a new two-dimensional (2D) model to predict follicular permeation, with integration into a recently reported multi-scale model of transdermal permeation is presented. METHODS:The follicular pathway is modelled by diffusion in sebum. The mass transfer and partition properties of solutes in li...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Kattou P,Lian G,Glavin S,Sorrell I,Chen T

    更新日期:2017-10-01 00:00:00

  • Correction to: A Semi-Physiologically Based Pharmacokinetic Model Describing the Altered Metabolism of Midazolam Due to Inflammation in Mice.

    abstract::One of the authors has his name incorrectly indexed in PubMed and SpringerLink as "Laird Forrest M" (last name "Laird Forrest"). His name should index as "Forrest M. Laird" with last name as "Forrest". ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章,已发布勘误


    authors: Varkhede N,Patel N,Chang W,Ruterbories K,Forrest ML

    更新日期:2018-07-25 00:00:00

  • Fuzzy modeling of skin permeability coefficients.

    abstract:PURPOSE:The purpose of this work was to determine whether a new modeling methodology using fuzzy logic c an predict skin permeability coefficients that are given compound descriptors that have been proven to affect percutaneous penetration. METHOD:Three fuzzy inference models were developed using subtractive clusterin...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Pannier AK,Brand RM,Jones DD

    更新日期:2003-02-01 00:00:00

  • Validation of a peptide map for recombinant porcine growth hormone and application to stability assessment.

    abstract::A reverse-phase HPLC method for the analysis of tryptic digests of recombinant porcine growth hormone (pGH) has been developed and validated. Digestion was performed at 4 degrees C for a 20-hr period with TPCK-treated trypsin at a 1:20 (w/w) trypsin:pGH ratio. Gradient elution HPLC, using an Aquapore RP300 C8 column, ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Charman SA,McCrossin LE,Charman WN

    更新日期:1993-10-01 00:00:00

  • Design and Synthesis of Polymer Prodrugs for Improving Water-Solubility, Pharmacokinetic Behavior and Antitumor Efficacy of TXA9.

    abstract:PURPOSE:TXA9, a novel cardiac glycoside, has a potent anti-proliferative effect against A549 human lung cancer cells, however, possesses a poor water-solubility and a rapid metabolic rate in vivo which limited the further development of TXA9. To overcome the shortcomings of TXA9, four polymer prodrugs of TXA9 were desi...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Li Y,Ye C,Cai C,Zhao M,Han N,Liu Z,Zhai J,Yin J

    更新日期:2020-03-12 00:00:00

  • In situ determination of delavirdine mesylate particle size in solid oral dosage forms.

    abstract:PURPOSE:The in-situ particle size of delavirdine mesylate in dry mix and tablets was determined. METHODS:Optical microscopy and fluorescence microscopy combined with image analysis were used for qualitative and quantitative measurements. RESULTS:Using optical microscopy, it was demonstrated qualitatively that fragmen...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: White JG

    更新日期:1999-04-01 00:00:00

  • Mechanical properties of dry and wet cellulosic and acrylic films prepared from aqueous colloidal polymer dispersions used in the coating of solid dosage forms.

    abstract::The mechanical properties of dry and wet polymeric films prepared from various aqueous polymeric dispersions were evaluated by a puncture test. They were studied with respect to type of polymer dispersion [cellulosic: Aquacoat and Surelease; acrylic: Eudragit NE, L, RS, and RL 30 D], plasticizer type (water-soluble or...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Bodmeier R,Paeratakul O

    更新日期:1994-06-01 00:00:00

  • Lipid absorption triggers drug supersaturation at the intestinal unstirred water layer and promotes drug absorption from mixed micelles.

    abstract:PURPOSE:To evaluate the potential for the acidic intestinal unstirred water layer (UWL) to induce drug supersaturation and enhance drug absorption from intestinal mixed micelles, via the promotion of fatty acid absorption. METHODS:Using a single-pass rat jejunal perfusion model, the absorptive-flux of cinnarizine and ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Yeap YY,Trevaskis NL,Porter CJ

    更新日期:2013-12-01 00:00:00

  • The effect of cyclodextrins on the stability of peptides in nasal enzymic systems.

    abstract::Leucine enkephalin (YGGFL) undergoes rapid degradation in sheep nasal mucosa to yield GGFL which is further degraded to FL. The activity of the nasal mucosal homogenate against YGGFL and GGFL (t1/2 12 and 7 min) was significantly greater than that observed with a nasal wash fluid (t1/2 40 and 13 min). The effect of cy...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Irwin WJ,Dwivedi AK,Holbrook PA,Dey MJ

    更新日期:1994-12-01 00:00:00

  • Systemic administration of TerplexDNA system: pharmacokinetics and gene expression.

    abstract:PURPOSE:The aim of this study is to extend our previous studies to investigate the TerplexDNA synthetic gene carrier system in pharmacokinetics, biodistribution, and gene expression in major organs after systemic administration. METHODS:The stability of the TerplexDNA system was analyzed in vitro with a serum incubati...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Yu L,Suh H,Koh JJ,Kim SW

    更新日期:2001-09-01 00:00:00

  • Pharmacodynamics of insulin following intravenous and enteral administrations of porcine-zinc insulin to rats.

    abstract::Previous work from this laboratory showed site-dependent variations in the apparent permeability of insulin as measured using the everted rat gut sac technique, with the greatest permeability in the distal jejunum and the lowest in the duodenum (5). To quantify better the rate and extent of insulin absorption from the...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Schilling RJ,Mitra AK

    更新日期:1992-08-01 00:00:00

  • Polymeric materials for theranostic applications.

    abstract::Nanotechnology has continuously contributed to the fast development of diagnostic and therapeutic agents. Theranostic nanomedicine has encompassed the ongoing efforts on concurrent molecular imaging of biomarkers, delivery of therapeutic agents, and monitoring of therapy response. Among these formulations, polymer-bas...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章,评审


    authors: Wang Z,Niu G,Chen X

    更新日期:2014-06-01 00:00:00

  • Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function.

    abstract:PURPOSE:In this study, we aimed to illustrate the utility of fractional radiofrequency (RF) that generated microchannels in the skin, allowing delivery of peptide and siRNA via the skin. The mechanisms involved in the correlation between macromolecule permeation and skin structure were also elucidated. METHODS:The mor...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Lee WR,Shen SC,Sun CK,Aljuffali IA,Suen SY,Lin YK,Wang JJ,Fang JY

    更新日期:2015-05-01 00:00:00

  • Sustainable Dissolution Performance of a Carrier Tailored Electrospun.

    abstract:PURPOSE:This study aims to conduct an impact investigation in the hydrophobic-hydrophilic balance as an important factor for dissolution improvement of a hydrophilic carrier-based solid dispersion system. METHODS:Polymeric carriers with different hydrophobic to hydrophilic ratios were used to prepare several electrosp...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Teoh XY,Yeoh Y,Yoong LK,Chan SY

    更新日期:2020-01-07 00:00:00

  • Influence of process conditions on the crystallization and transition of metastable mannitol forms in protein formulations during lyophilization.

    abstract:PURPOSE:To study the impact of different process conditions and formulation compositions on metastable mannitol forms in protein formulations during lyophilization. METHODS:Mannitol was studied with and without other formulation components. A cryostage was used to mimic the different processing steps during lyophiliza...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Cao W,Xie Y,Krishnan S,Lin H,Ricci M

    更新日期:2013-01-01 00:00:00

  • Assessment of the potential skin irritation of lysine-derivative anionic surfactants using mouse fibroblasts and human keratinocytes as an alternative to animal testing.

    abstract:PURPOSE:The aim of this study was to identify new surfactants with low skin irritant properties for use in pharmaceutical and cosmetic formulations, employing cell culture as an alternative method to in vivo testing. In addition, we sought to establish whether potential cytotoxic properties were related to the size of ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Sanchez L,Mitjans M,Infante MR,Vinardell MP

    更新日期:2004-09-01 00:00:00

  • Nanoparticulate Impurities in Pharmaceutical-Grade Sugars and their Interference with Light Scattering-Based Analysis of Protein Formulations.

    abstract:PURPOSE:In the present study we investigated the root-cause of an interference signal (100-200 nm) of sugar-containing solutions in dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) and its consequences for the analysis of particles in biopharmaceutical drug products. METHODS:Different sugars as ...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Weinbuch D,Cheung JK,Ketelaars J,Filipe V,Hawe A,den Engelsman J,Jiskoot W

    更新日期:2015-07-01 00:00:00

  • A population pharmacokinetic model for montelukast disposition in adults and children.

    abstract:PURPOSE:The purpose was to develop a population pharmacokinetic model for montelukast after intravenous administration. Clinical trial simulations were conducted using the model developed to identify the lowest intravenous dose in 6- to 14-year-old children that would give montelukast systemic exposures that were compa...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章,随机对照试验


    authors: Ramakrishnan R,Migoya E,Knorr B

    更新日期:2005-04-01 00:00:00

  • Alterations in neuronal transport but not blood-brain barrier transport are observed during gamma-hydroxybutyrate (GHB) sedative/hypnotic tolerance.

    abstract:PURPOSE:To investigate if gamma-Hydroxybutyrate (GHB) tolerance is mediated by alterations in GHB systemic pharmacokinetics, transport (blood brain barrier (BBB) and neuronal) or membrane fluidity. MATERIALS AND METHODS:GHB tolerance in rats was attained by repeated GHB administration (5.31 mmol/kg, s.c., QD for 5 day...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Bhattacharya I,Raybon JJ,Boje KM

    更新日期:2006-09-01 00:00:00

  • The effect of engineered mannitol-lactose mixture on dry powder inhaler performance.

    abstract:PURPOSE:To co-crystallise mannitol and lactose with a view to obtaining crystals with more favourable morphological features than either lactose or mannitol alone, suitable for use as carriers in formulations for dry powder inhalers (DPIs) using simultaneous engineering of lactose-mannitol mixtures. METHODS:Mannitol a...

    journal_title:Pharmaceutical research

    pub_type: 杂志文章


    authors: Kaialy W,Larhrib H,Martin GP,Nokhodchi A

    更新日期:2012-08-01 00:00:00