Optical purity determination of threo-methylphenidate hydrochloride using a chiral europium nuclear magnetic resonance (NMR) shift reagent.

Abstract:

:A 1H-NMR spectroscopic method for the determination of the optical purity of threo-methylphenidate hydrochloride is presented. Complexation of the free-base form of the substrate with a chiral Eu(III) shift reagent resulted in two distinct enantiomeric ester methyl proton signals of utility for quantitative work. The accuracy of the method was validated by analyzing six synthetic mixtures of various proportions of (+)- and (-)-threo-methylphenidate. In addition to yielding assay results that were in close agreement with the known weights of each enantiomer in the mixture, and a mean recovery of the (-)-threo-enantiomer of better than 99.0%, the proposed method was capable of accurately measuring as little as 2% of one enantiomer in the presence of the other.

journal_name

Pharm Res

journal_title

Pharmaceutical research

authors

Hanna GM,Lau-Cam CA

doi

10.1023/a:1015815605583

subject

Has Abstract

pub_date

1990-07-01 00:00:00

pages

726-9

issue

7

eissn

0724-8741

issn

1573-904X

journal_volume

7

pub_type

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