Abstract:
:The efficiency and high specificity of tobacco etch virus protease (TEVp) has made it widely used for cleavage of recombinant fusion proteins. However, TEVp suffers from a few intrinsic defects such as self-cleavage, poorly expressed in E. coli and less soluble. So some mutants were designed to improve it, such as S219V, T17S/N68D/I77V and L56V/S135G etc. MD simulations for the WT TEVp and its mutants were performed to explore the underlying dynamic effects of mutations on TEVp. Although the globular domains are fairly conserved, the three mutations have diverse effects on the dynamics properties of TEVp, including the elongation of β-sheet, conversion of loop to helix and the flexibility of active core. Our present study indicates that the three mutants for TEVp can change their secondary structure and tend to form more helixes and sheets to improve stability. The study also helps us to understand the effects of some mutations on TEVp, provides us insights into the change of them at the atomic level and gives a potential rational method to design an improved protein.
journal_name
J Mol Modeljournal_title
Journal of molecular modelingauthors
Wang Y,Zhu GF,Ren SY,Han YG,Luo Y,Du LFdoi
10.1007/s00894-013-1930-9subject
Has Abstractpub_date
2013-11-01 00:00:00pages
4865-75issue
11eissn
1610-2940issn
0948-5023journal_volume
19pub_type
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journal_title:Journal of molecular modeling
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journal_title:Journal of molecular modeling
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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更新日期:2019-08-19 00:00:00
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journal_title:Journal of molecular modeling
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更新日期:2014-03-01 00:00:00
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更新日期:2009-04-01 00:00:00
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更新日期:2013-05-01 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
doi:10.1007/s00894-008-0285-0
更新日期:2008-08-01 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
doi:10.1007/s00894-017-3236-9
更新日期:2017-03-01 00:00:00
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更新日期:2017-11-04 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Journal of molecular modeling
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更新日期:2016-05-01 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
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更新日期:2011-12-01 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
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更新日期:2018-06-14 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
doi:10.1007/s00894-011-1015-6
更新日期:2011-12-01 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
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更新日期:2019-06-10 00:00:00
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journal_title:Journal of molecular modeling
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更新日期:2017-07-01 00:00:00
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journal_title:Journal of molecular modeling
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更新日期:2013-12-01 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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journal_title:Journal of molecular modeling
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更新日期:2015-04-01 00:00:00
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journal_title:Journal of molecular modeling
pub_type: 杂志文章
doi:10.1007/s00894-013-1816-x
更新日期:2013-06-01 00:00:00