Abstract:
PURPOSE:Voltage-gated potassium ion channels have long been implicated in gastrointestinal motility. Recent studies have highlighted the role of voltage-gated channel subfamily G member 3 (KCNG3) and 4 (KCNG4) genes in the electrical functioning of interstitial cells of Cajal and PDGFRα+ cells of the mouse colon. We designed this study to investigate KCNG3 and KCNG4 expression in the normal human colon and in Hirschsprung's disease (HSCR). METHODS:HSCR tissue specimens (n = 6) were collected at the time of pull-through surgery, while control samples were obtained at the time of colostomy closure in patients with imperforate anus (n = 6). qRT-PCR analysis was undertaken to quantify KCNG3 and KCNG4 gene expression, and immunolabelling of KCNG3 and KCNG4 proteins was visualized using confocal microscopy. RESULTS:qRT-PCR analysis revealed significant downregulation of the KCNG3 and KCNG4 genes in both aganglionic and ganglionic HSCR specimens compared to controls (p < 0.05). Confocal microscopy revealed KCNG3 and KCNG4 expression within neurons, ICC and PDGFRα+ cells of the myenteric plexus and smooth muscle layers, with a reduction in both proteins in aganglionic and ganglionic HSCR colon compared to controls. CONCLUSION:KCNG3 and KCNG4 gene expression is significantly downregulated in HSCR colon, suggesting a role for these genes in colonic motility. KCNG3 and KCNG4 downregulation within ganglionic specimens highlights the physiologically abnormal nature of this segment in HSCR patients.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
O'Donnell AM,Nakamura H,Tomuschat C,Marayati NF,Puri Pdoi
10.1007/s00383-018-4394-2subject
Has Abstractpub_date
2019-02-01 00:00:00pages
193-197issue
2eissn
0179-0358issn
1437-9813pii
10.1007/s00383-018-4394-2journal_volume
35pub_type
杂志文章abstract::Migration of particulate matter from plastic tubing and solid plastic implants has been documented in a number of studies, including some with the use of cardiac bypass, haemodialysis, and pump-assisted intravenous infusions. In order to ascertain whether silicone embolisation occurs when children have an Ivac 560 pum...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01194802
更新日期:1997-01-01 00:00:00
abstract:OBJECTIVE:Surgical repair of pectus excavatum (PE) in childhood is a well-established procedure. Previously used operative techniques to correct PE were largely based on the Ravitch technique. Since about 10 years, the minimally invasive repair (MIRPE) by Nuss is well established. Conservative treatment with the vacuum...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2843-7
更新日期:2011-06-01 00:00:00
abstract:PURPOSE:To study the effects of frenulotomy on nipple pain, latch and the success in exclusive breastfeeding (EBF) at 3-month follow-up. METHODS:A prospective cross-sectional study of 328 mother-infant pairs with both tongue-tie and breastfeeding problems was performed. Nipple pain and latch were evaluated using numer...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3952-8
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abstract:AIM:Although liver abscess (LA) is prevalent worldwide, especially in developing countries, there is a paucity of data regarding the protocol for management of LA in children. The aim of this study was to analyze the outcome of a protocol-based management of pediatric LA from a single institution. MATERIALS AND METHOD...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-4009-8
更新日期:2017-02-01 00:00:00
abstract::A case of an epigastric giant-cell fibroblastoma is reported in a 6-year-old girl who had undergone a bone-marrow transplant for severe combined immunodeficiency secondary to adenosine deaminase deficiency. A small subcutaneous nodule had been excised from the epigastrium at age 12 months. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0827-y
更新日期:2003-08-01 00:00:00
abstract::In many very-low-birth-weight (VLBW) infants the ductus arteriosus fails to close spontaneously, and they subsequently develop signs and symptoms of poor tissue perfusion and heart failure. This study evalutes the results of early surgical closure of patent ductus arteriosus (PDA). We retrospectively reviewed the reco...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000515
更新日期:2001-07-01 00:00:00
abstract::Congenital hepatic cysts are rare lesions of infancy. While operative management and outcomes have been extensively studied in adult patients with hepatic cysts, data in pediatric patients are limited. We discuss our experience in an infant and review relevant literature regarding operative technique and surgical outc...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-013-3312-x
更新日期:2013-08-01 00:00:00
abstract::Since January 2006, robotic assistance has been used for performing minimal invasive laparoscopic fundoplications in children. These patients were compared with those operated on with either the open surgical technique or the laparoscopic procedure. The first six children operated on with a fundoplication using the op...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1817-2
更新日期:2007-02-01 00:00:00
abstract::Cryptorchidism is a common paediatric complaint and is traditionally managed surgically by orchidopexy. We aimed to prospectively review the success of the Bianchi high scrotal incision technique for performing orchidopexy in boys with palpable non-descended testis. A total of 206 orchidopexies were performed in 85 pa...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2149-1
更新日期:2008-06-01 00:00:00
abstract::Incarcerated inguinal hernias in infants are commonly encountered. Reduction with intravenous sedation using morphine and related drugs carries risks of apnoea and/or respiratory arrest, especially in small, ex-premature babies who have an increased incidence of incarceration and thus opiate use is best avoided. Cauda...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1534-2
更新日期:2005-09-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3946-6
更新日期:2016-10-01 00:00:00
abstract::Nine babies with pure oesophageal atresia were treated in our institution in the years 1979-1996. All received a feeding gastrostomy as their initial operation. After initiation of gastrostomy feeds seven (78%) developed gastric complications, including two posterior gastric perforations (one fatal). We propose that t...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050640
更新日期:1999-01-01 00:00:00
abstract::The Thomsen-Friedenreich cryptantigen (TCA) is located on the surface of all red cells, but is concealed by a layer of neuraminic acid. When bacteria that produce neuraminidase disrupt this coating, the TCA can be exposed and activated. If blood products containing antibody to the TCA are subsequently administered, ha...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050488
更新日期:1998-12-01 00:00:00
abstract::Polyps in gastrointestinal tract are mostly benign and result from hamartomas or lymphoid hyperplasia of submucosa. They usually occur as isolated lesions in children and are referred to as juvenile polyps. Multiple polyps with inherited origin are called polyposis and can be seen as a part of the syndrome. The polyps...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-020-04621-3
更新日期:2020-04-01 00:00:00
abstract::High ligation of spermatic vessels (HLSV) and low ligation of spermatic vessels (LLSV) have been described in the surgical treatment of high undescended testis. Testicular capillary blood flow utilizing laser Doppler flowmetry after these maneuvers has not been studied thus far; hence, this study was designed. Thirty ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-1018-1
更新日期:2003-07-01 00:00:00
abstract::This article describes five cases of congenital epulis, a rare and benign swelling in the mouth of a newborn, which is not widely known. We present five cases: four cases presented as single pedunculated nodules of the gingiva and in one case two nodules were present. Of all, 50% were located at the maxilla. Excision ...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-008-2304-8
更新日期:2009-02-01 00:00:00
abstract:PURPOSE:For International Neuroblastoma Staging System (INSS) stages III or IV neuroblastoma (intermediate or high risk), complete excision of the primary tumor is not always feasible. Most current studies on the treatment outcome of these patients have reported on the complete excision status. The aim of this study is...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2417-8
更新日期:2009-09-01 00:00:00
abstract:AIM OF THE STUDY:The high morbidity and mortality in congenital diaphragmatic hernia (CDH) is attributed to pulmonary hypoplasia and persistent pulmonary hypertension (PH). PH is characterized by increased pulmonary artery smooth muscle cell (SMC) proliferation, suppressed apoptosis as well as endothelial dysfunction. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3606-7
更新日期:2014-12-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-017-4062-y
更新日期:2017-06-01 00:00:00
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journal_title:Pediatric surgery international
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doi:10.1007/s00383-015-3761-5
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-019-04472-7
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:Recognition of physical child abuse is imperative for ensuring children's safety. Screening tools (ST) may increase identification of physical abuse; however, the extent of their use is unknown. This study assessed use of STs for physical abuse in children's hospitals and determined attitudes regarding STs. ME...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3916-z
更新日期:2016-08-01 00:00:00
abstract::The aim of this study was to determine whether barium enema reduction (BER) is safe and effective in patients with a long duration of intussusception. Over the last 17 years, 104 patients were admitted to our hospital with a diagnosis of intussusception. All except 1 with peritonitis underwent BER primarily. Of the 10...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050526
更新日期:1999-01-01 00:00:00
abstract::It has been assumed that only 10% of medical interventions are supported by solid scientific evidence. The aim of this study was to determine the type of research evidence supporting operations in a tertiary referral paediatric surgical unit. All patients admitted over a 4-week period to two surgical firms were enroll...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050332
更新日期:1998-07-01 00:00:00
abstract:AIM OF STUDY:Few studies have evaluated the incidence of familial vesicoureteral reflux (VUR) among multiple gestation births. The purpose of this study was to determine the incidence and characteristics of VUR in twins in a large cohort of families with VUR. METHODS:Between 1998 and 2010, data were collected on 251 f...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2821-0
更新日期:2011-05-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2899-z
更新日期:2011-08-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3879-0
更新日期:2016-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-05-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050667
更新日期:1999-01-01 00:00:00
abstract::Foreign-body (FB) aspirations account for a reasonable percentage of respiratory emergencies in young children. Whereas 67% of aspirated FBs are of organic origin, metallic-ferromagnetic FBs are observed less frequently. We report the use of a wire with a magnetic tip fitting into the working channel of a rigid bronch...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01076970
更新日期:1997-07-01 00:00:00