Abstract:
:High-quality and well-annotated biorepositories are needed to better understand the pathophysiology and biologic mechanisms of chronic pancreatitis (CP) and its consequences. We report a methodology for the development of a robust standard operating procedure (SOP) for a biorepository based on the experience of the clinical centers within the consortium to study Chronic Pancreatitis, Diabetes and Pancreas Cancer Clinical Centers (CPDPC), supported by the National Cancer Institute and the National Institute for Diabetes and Digestive and Kidney Diseases as a unique multidisciplinary model to study CP, diabetes, and pancreatic cancer in both children and adults. Standard operating procedures from the CPDPC centers were evaluated and consolidated. The literature was reviewed for standard biorepository operating procedures that facilitated downstream molecular analysis. The existing literature on biobanking practices was harmonized with the SOPs from the clinical centers to produce a biorepository for pancreatic research. This article reports the methods and basic principles behind the creation of SOPs to develop a biorepository for the CPDPC. These will serve as a guide for investigators developing biorepositories in pancreas research. Rigorous and meticulous adherence to standardized biospecimen collection will facilitate investigations to better understand the pathophysiology and biologic mechanisms of CP, diabetes, and pancreatic cancer.
journal_name
Pancreasjournal_title
Pancreasauthors
Fisher WE,Cruz-Monserrate Z,McElhany AL,Lesinski GB,Hart PA,Ghosh R,Van Buren G,Fishman DS,Rinaudo JAS,Serrano J,Srivastava S,Mace T,Topazian M,Feng Z,Yadav D,Pandol SJ,Hughes SJ,Liu RY,Lu E,Orr R,Whitcomb DC,Abdoi
10.1097/MPA.0000000000001171subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1213-1221issue
10eissn
0885-3177issn
1536-4828pii
00006676-201811000-00009journal_volume
47pub_type
杂志文章相关文献
PANCREAS文献大全abstract::Cystic lesions of the pancreas include inflammatory pseudocysts, serous cystadenomas, and mucinous tumors, some of which are malignant. Preoperative clinical and radiological parameters are unreliable and may result in incorrect diagnosis and inappropriate treatment. Cyst fluid analysis for cytology, viscosity, carcin...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199505000-00004
更新日期:1995-05-01 00:00:00
abstract::Human pure pancreatic juice (PPJ) and serum were analyzed for free fatty acids (FFAs) to study whether the damage to pancreatic cell membranes in pancreatitis is reflected as abnormal FFAs concentration and composition. Patients consisted of 13 normal controls, 7 patients with acute pancreatitis (AP) in remission, and...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-198804000-00017
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVES:This analysis investigated nomogram use to evaluate metastatic pancreatic cancer prognosis. METHODS:Thirty-four baseline factors were examined in the Metastatic Pancreatic Adenocarcinoma Clinical Trial (MPACT) (nab-paclitaxel plus gemcitabine vs gemcitabine) data set. Factors significantly (P < 0.1) associa...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001563
更新日期:2020-07-01 00:00:00
abstract::This study was performed to assess the effects of misoprostol, a synthetic prostaglandin E1 analog, on cerulein-induced pancreatitis. Per group of 10 each, male Wistar rats received either cerulein (2.5 micrograms/kg/h subcutaneously), cerulein and misoprostol (500 micrograms/kg intraperitoneally at 0 and 4 h), or sal...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199003000-00008
更新日期:1990-03-01 00:00:00
abstract:OBJECTIVES:We evaluated carcinoid syndrome (CS) symptoms and the real-world effectiveness of telotristat ethyl (TE) among patients with ≤3 bowel movements (BM) per day. METHODS:Patients with CS initiating TE between March and November 2017 could participate in a nurse support program collecting demographic and CS symp...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001496
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:Previously, we showed that diazoxide (DZ), an effective ischemic preconditioning agent, protected rodent pancreas against ischemia-reperfusion injury. Here, we further investigate whether DZ supplementation to University of Wisconsin (UW) solution during pancreas procurement and islet isolation has similar c...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001557
更新日期:2020-05-01 00:00:00
abstract::Pancreatic acinar cells within the islets of Langerhans in human pancreas were investigated both histopathologically and electron microscopically. Acinar cells inside the islets of Langerhans were observed in all 50 adult cases investigated. Between such acinar cells and endocrine tissues, no reticular fiber was obser...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199409000-00004
更新日期:1994-09-01 00:00:00
abstract:OBJECTIVES:Inducible nitric oxide synthase (iNOS) activity is increased in experimental acute pancreatitis. The aim of this study was to evaluate treatment with the selective iNOS inhibitors AR-C (AR-C102222AA) and L-NIL (L-N6-(1-iminoethyl)-lysine) in experimental acute pancreatitis. METHODS:Acute pancreatitis was in...
journal_title:Pancreas
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Recently, the landscape of the diagnosis and treatment of patients with well-differentiated gastrointestinal/pancreatic neuroendocrine tumors (previously referred to as carcinoid tumors) has changed dramatically. We will need to work with all of the stakeholders including clinicians, patients, regulatory agencies, and...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000620
更新日期:2016-07-01 00:00:00
abstract::The effects of manganese (Mn2+) on phosphorylation activity in the rat pancreas were examined in an in vitro phosphorylation assay and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of endogenous proteins. Several histones were phosphorylated in the presence of pancreatic supernatant obtained ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199009000-00015
更新日期:1990-09-01 00:00:00
abstract:OBJECTIVES:This study aimed to evaluate magnetic resonance imaging findings of autoimmune pancreatitis (AIP) and to find radiological patterns that could differentiate type 1 and type 2 AIP. METHODS:Eighty-four patients with diagnosis of AIP were enrolled. Image analysis included pancreatic signal intensity abnormalit...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001142
更新日期:2018-10-01 00:00:00
abstract:OBJECTIVE:Systemic complications in alcoholic pancreatitis are supposed to be aggravated by inflammatory liver damage. Resident macrophages including hepatic Kupffer cells play a pivotal role in mediating systemic complications in severe necrotizing pancreatitis (SNP). The aim of this study was to evaluate the effects ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181bd6470
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVES:Cyclooxygenase 2 (COX-2) and vascular endothelial growth factor (VEGF), often coexpressed in cancer, are associated with poor prognosis. However, results from pancreatic cancer trials of their inhibitors were disappointing. This study delineated the role of COX-2 and nonsteroidal anti-inflammatory drugs in a...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181c6575d
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:Pancreatic ischemia-reperfusion (IR) has a key role in pancreas surgery and transplantation. Most experimental models evaluate the normothermic phase of the IR. We proposed a hypothermic model of pancreas IR to evaluate the benefic effects of the cold ischemic phase. METHODS:We performed a reproducible model...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000150
更新日期:2014-08-01 00:00:00
abstract::In this article, we introduce our rapid-production model for pancreatic duct adenocarcinomas and describe the mechanisms of pancreatic duct carcinogenesis so far elucidated in Syrian golden hamsters. It is evident that a series of histogenetic steps are involved, leading from hyperplasia through atypical hyperplasia t...
journal_title:Pancreas
pub_type: 杂志文章,评审
doi:10.1097/00006676-199804000-00015
更新日期:1998-04-01 00:00:00
abstract::Computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET) are sophisticated modalities typically used in the second-line diagnosis following routine clinical practice. Among them, CT is regarded as the standard imaging in diagnosing pancreatic cancer at present in Japan due to ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200404000-00011
更新日期:2004-04-01 00:00:00
abstract:OBJECTIVES:The contribution of overexpressed microRNA-21 and -221 (miR-21 and miR-221) to the malignant phenotype was determined by inhibiting these miRNAs using antisense oligonucleotides. METHODS:The effects of antisense to miR-21 and miR-221 on cell proliferation, cell cycle arrest, induction of apoptosis, combinat...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181ba82e1
更新日期:2009-10-01 00:00:00
abstract::Free radicals have been implicated in beta-cell destruction. pancreatic antioxidant enzymatic defenses provide significant endogenous protection from these highly cytotoxic effector molecules. In our colony of BB rats, the reliability of peripheral blood lymphocyte count (PBLC) as a predictor of diabetes onset was ass...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199501000-00007
更新日期:1995-01-01 00:00:00
abstract::The recent publication of evidence-based guidelines by the American Gastroenterological Association for the management of the incidental pancreatic cyst has stimulated considerable discussion among clinicians and researchers. The guidelines examined the evidence in an accompanying technical review defining current dia...
journal_title:Pancreas
pub_type: 杂志文章,评审
doi:10.1097/MPA.0000000000000832
更新日期:2017-07-01 00:00:00
abstract::Adenosine-5'-O-(2-thiodiphosphate) (ADP beta S), a P2y purinergic agonist, has been shown to be a potent insulin secretagogue on the isolated rat pancreas. In the present work the effects of ADP beta S on insulin somatostatin, and glucagon secretions were investigated in dogs. In vivo, in anesthetized fasted dogs, i.v...
journal_title:Pancreas
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to identify differentially expressed proteins among various stages of pancreatic ductal adenocarcinoma (PDAC) by shotgun proteomics using nano-liquid chromatography coupled tandem mass spectrometry and stable isotope dimethyl labeling. METHODS:Differentially expressed proteins were...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000388
更新日期:2016-01-01 00:00:00
abstract::Pancreatic ductal adenocarcinoma is characterized by a high rate of activating mutations involving codon 12 of the K-ras protooncogene. As a means of ras-targeted intervention, the effects of enhanced Krev-1 gene expression on the growth and tumorigenicity of the hamster pancreatic adenocarcinoma cell line PC-1 were e...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199805000-00006
更新日期:1998-05-01 00:00:00
abstract:INTRODUCTION:Islet cell transplantation offers a new approach for the treatment of diabetes. Before transplantation, immunomodulation procedures are conducted to reduce the immunogenicity of the pancreatic islets. AIM:To study the effect of cyclophosphamide pretreatment and culture on the xenograft survival of monkey ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200204000-00005
更新日期:2002-04-01 00:00:00
abstract::Type I diabetes is characterized by destruction of insulin-producing beta-islet cells in the pancreas resulting in hyperglycemia and associated morbidity. The successful treatment of diabetes by transplanted islets has resulted in renewed efforts to identify methods to augment islet availability. One approach is to id...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200407000-00064
更新日期:2004-07-01 00:00:00
abstract:OBJECTIVE:Our study aim was to determine encapsulated islet graft viability in an omentum pouch and the effect of fibroblast growth factor 1 (FGF-1) released from our redesigned alginate microcapsules on the function of the graft. METHODS:Isolated rat islets were encapsulated in an inner core made with 1.5% low-viscos...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000107
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVE:Epithelial-mesenchymal transition may interfere with the differentiation of cultured pancreatic acinar cells toward endocrine cells. Therefore, it will be important to investigate into detail the reprogramming of human pancreatic acinar cells toward a mesenchymal phenotype: the association with acinoductal tr...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000154
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVES:The objectives of the study were to compare endoscopic ultrasonography (EUS) and magnetic resonance cholangiopancreatography (MRCP) in the etiological diagnosis of patients initially diagnosed with idiopathic acute pancreatitis and to determine the clinical and analytical factors related to the end result of...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e318201654a
更新日期:2011-03-01 00:00:00
abstract::Protein phosphatases have recently been recognized to represent an important, independently regulated portion of cellular signaling cascades. Although reversible phosphorylation of multiple pancreatic proteins has been described, suggesting a role for these enzymes, little is known about the characteristics of protein...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199407000-00002
更新日期:1994-07-01 00:00:00
abstract::We examined whether pancreas grafts preserved by the two-layer method maintained adenine nucleotides pool as substrates for ATP synthesis before reperfusion and had the ability to recover pancreatic tissue perfusion and synthesize ATP promptly after reperfusion. After preservation by the two-layer cold storage method ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199704000-00011
更新日期:1997-04-01 00:00:00
abstract:OBJECTIVES:Glucagon-like peptide-1 (GLP-1) is known to stimulate glucose-dependent insulin production and secretion by pancreatic beta-cells. Preliminary evidence suggests that GLP-1 may also influence endocrine differentiation from pancreatic progenitor cells. Additionally, TGF-beta signaling can also control endocrin...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/01.mpa.0000172566.70619.58
更新日期:2005-08-01 00:00:00