Inhibition by gossypol of cyclic AMP production in mouse Leydig cells.

Abstract:

:Gossypol has been shown to inhibit steroidogenesis in leydig cells. This study examined the mechanism of this action by investigating the effect of gossypol on leydig cell hCG receptor binding, adenylate cyclase activity and cyclic AMP production in leydig cells. Gossypol had no effect on hCG binding to cell membranes but inhibited LH-stimulated cyclic AMP production in whole purified leydig cells. It also reduced the stimulation caused by LH, Gpp(NH)p, forskolin and fluoride in membranes from leydig cells and also from liver. It is therefore possible that gossypol affects cyclic AMP production at the level of ATP conversion to cyclic AMP, although effects on the G-protein and catalytic subunit cannot be ruled out.

journal_name

Contraception

journal_title

Contraception

authors

Pearce S,Sufi SB,O'Shaughnessy PJ,Donaldson A,Jeffcoate SL

doi

10.1016/s0010-7824(86)80019-1

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

639-46

issue

6

eissn

0010-7824

issn

1879-0518

pii

S0010-7824(86)80019-1

journal_volume

34

pub_type

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    pub_type: 杂志文章

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