MiR-409-3p and MiR-1896 co-operatively participate in IL-17-induced inflammatory cytokine production in astrocytes and pathogenesis of EAE mice via targeting SOCS3/STAT3 signaling.

Abstract:

:Th17 cells and interleukin-17 (IL-17) have been found to play an important role in the pathology of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Response to IL-17, reactive astrocytes accompany with immune cells infiltration and axonal damage in MS/EAE. However, the role and the regulatory mechanism of IL-17-activated astrocytes in inflammation and in the EAE process still remain largely unknown. Here, we elucidated that miR-409-3p and miR-1896, as co-upregulated microRNAs in activated astrocytes and in EAE mice, targeted suppressor of cytokine signaling proteins 3 (SOCS3). Overexpression of miR-409-3p or miR-1896 significantly reduced SOCS3 expression and increased phosphorylation of STAT3 as well as induced the inflammatory cytokines production (IL-1β, IL-6, IP-10, MCP-1, and KC), CD4+ T cells migration and demyelination, in turn aggravating EAE development. Importantly, the effects of co-overexpression of miR-409-3p and miR-1896 in vitro or in vivo are strongly co-operative. In contrast, simultaneously silenced miR-409-3p and miR-1896 co-operatively ameliorates inflammation and demyelination in the central nervous system of EAE mice. Collectively, our findings highlight that miR-409-3p and miR-1896 co-ordinately promote the production of inflammatory cytokines in reactive astrocytes through the SOCS3/STAT3 pathway and enhance reactive astrocyte-directed chemotaxis of CD4+ T cells, leading to aggravate pathogenesis in EAE mice. Co-inhibition of miR-409-3p and miR-1896 may be a therapeutic target for treating MS and neuroinflammation.

journal_name

Glia

journal_title

Glia

authors

Liu X,Zhou F,Yang Y,Wang W,Niu L,Zuo D,Li X,Hua H,Zhang B,Kou Y,Guo J,Kong F,Pan W,Gao D,Meves JM,Sun H,Xue M,Zhang Q,Wang Y,Tang R

doi

10.1002/glia.23530

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

101-112

issue

1

eissn

0894-1491

issn

1098-1136

journal_volume

67

pub_type

杂志文章

相关文献

GLIA文献大全
  • Differential contributions of microglial and neuronal IKKβ to synaptic plasticity and associative learning in alert behaving mice.

    abstract::Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning, and memory is not fully understood. In this study, we used mice with partial depletion of IκB kinase β, the main activating kinase in the in...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22756

    authors: Kyrargyri V,Vega-Flores G,Gruart A,Delgado-García JM,Probert L

    更新日期:2015-04-01 00:00:00

  • Repetitive injury and absence of monocytes promote astrocyte self-renewal and neurological recovery.

    abstract::Unlike microglia and NG2 glia, astrocytes are incapable of migrating to sites of injury in the posttraumatic cerebral cortex, instead relying on proliferation to replenish their numbers and distribution in the affected region. However, neither the spectrum of their proliferative repertoire nor their postinjury distrib...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23893

    authors: Lange Canhos L,Chen M,Falk S,Popper B,Straub T,Götz M,Sirko S

    更新日期:2021-01-01 00:00:00

  • Müller cell swelling, glutamate uptake, and excitotoxic neurodegeneration in the isolated rat retina.

    abstract::We characterized morphological effects of the endogenous excitotoxin, glutamate in ex vivo retinal segments prepared from 30-day-old rats. Initial changes induced by glutamate consisted of reversible, sodium-dependent Müller cell swelling. This glial swelling was mimicked by glutamate transport substrates but not by i...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Izumi Y,Kirby CO,Benz AM,Olney JW,Zorumski CF

    更新日期:1999-02-15 00:00:00

  • Epidermal growth factor receptor in proliferating reactive glia following transient focal ischemia in the rat brain.

    abstract::Severe transient focal cerebral ischemia causes brain infarction with a strong glial reaction. We have studied whether postischemic reactive glial cells express epidermal growth factor receptor (EGFR) following middle cerebral artery occlusion in the rat. We have also looked for signs of proliferating activity, as EGF...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(199806)23:2<120::aid-glia3

    authors: Planas AM,Justicia C,Soriano MA,Ferrer I

    更新日期:1998-06-01 00:00:00

  • Effect of anandamide uptake inhibition in the production of nitric oxide and in the release of cytokines in astrocyte cultures.

    abstract::Astrocytes play a key role regulating aspects of inflammation in the central nervous system (CNS). Several enzymes, such as the inducible nitric oxide synthase (iNOS) or the cyclooxygenase-2 (COX-2), along with different inflammatory mediators such as the free radical nitric oxide (NO) or proinflammatory cytokines, ha...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20229

    authors: Ortega-Gutiérrez S,Molina-Holgado E,Guaza C

    更新日期:2005-11-01 00:00:00

  • Gap junction channels coordinate the propagation of intercellular Ca2+ signals generated by P2Y receptor activation.

    abstract::Astrocytes express gap junction proteins and multiple types of P2Y receptors (P2YRs) that contribute to the propagation of intercellular Ca(2+) waves (ICW). To gain access to the role played by gap junctional communication in ICW propagation generated by P2YR activation, we selectively expressed P2Y(1,2,4)R subtypes a...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20071

    authors: Suadicani SO,Flores CE,Urban-Maldonado M,Beelitz M,Scemes E

    更新日期:2004-11-15 00:00:00

  • Differential regulation of trophic and proinflammatory microglial effectors is dependent on severity of neuronal injury.

    abstract::Microglial activation has been reported to promote neurotoxicity and also neuroprotective effects. A possible contributor to this dichotomy of responses may be the degree to which proximal neurons are injured. The aim of this study was to determine whether varying the severity of neuronal injury influenced whether mic...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20610

    authors: Lai AY,Todd KG

    更新日期:2008-02-01 00:00:00

  • Membrane-initiated effects of progesterone on calcium dependent signaling and activation of VEGF gene expression in retinal glial cells.

    abstract::Neurosteroids, such as progesterone, influence central nervous system development and function by regulating a broad spectrum of physiological processes. Here, we investigated membrane-initiated actions of progesterone in the retina and identified the membrane-associated progesterone receptor component 1 (PGRMC1). We ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20523

    authors: Swiatek-De Lange M,Stampfl A,Hauck SM,Zischka H,Gloeckner CJ,Deeg CA,Ueffing M

    更新日期:2007-08-01 00:00:00

  • Distribution of Aquaporin 4 in rodent spinal cord: relationship with astrocyte markers and chondroitin sulfate proteoglycans.

    abstract::Water balance between cells and extracellular compartments is essential for proper functioning of the central nervous system, as demonstrated by its perturbations in pathological conditions. Aquaporin 4 (AQP4) is the predominant water channel in brain and spinal cord, where it is present mainly on astrocytic endfeet c...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20196

    authors: Vitellaro-Zuccarello L,Mazzetti S,Bosisio P,Monti C,De Biasi S

    更新日期:2005-08-01 00:00:00

  • Role of glial amino acid transporters in synaptic transmission and brain energetics.

    abstract::This article reviews how the uptake of neurotransmitter by glial amino acid transporters limits the spatial spread of transmitter to preserve the independent operation of nearby synapses, temporally shapes postsynaptic currents, and regulates the effects of tonic transmitter release. We demonstrate the importance of a...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.20027

    authors: Marcaggi P,Attwell D

    更新日期:2004-08-15 00:00:00

  • Sub-micromolar increase in [Ca(2+)](i) triggers delayed exocytosis of ATP in cultured astrocytes.

    abstract::Astrocytes release a variety of transmitter molecules, which mediate communication between glial cells in the brain and modulate synaptic transmission. ATP is a major glia-derived transmitter, but the mechanisms and kinetics of ATP release from astrocytes remain largely unknown. Here, we combined epifluorescence and t...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20590

    authors: Pryazhnikov E,Khiroug L

    更新日期:2008-01-01 00:00:00

  • Bradykinin induces inositol 1,4,5-trisphosphate-dependent hyperpolarization in K+ M-current-deficient hybrid NL308 cells: comparison with NG108-15 neuroblastoma x glioma hybrid cells.

    abstract::External application of bradykinin (BK) to mouse neuroblastoma X mouse fibroblast hybrid NL308 cells and mouse neuroblastoma X rat glioma hybrid NG108-15 cells produced a transient outward (hyperpolarizing) current. In NG108-15 cells, BK also induced an inward (depolarizing) current associated with a decrease in input...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440030102

    authors: Higashida H,Okano Y,Hoshi N,Yada Y,Yokoyama S,Asaga T,Fu T,Nozawa Y

    更新日期:1990-01-01 00:00:00

  • The enteric glia: identity and functions.

    abstract::Enteric glial cells were first described at the end of the 19th century, but they attracted more interest from researchers only in the last decades of the 20th. Although, they have a different embryological origin, the enteric GLIA share many characteristics with astrocytes, the main glial cell type of the central ner...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.22795

    authors: Coelho-Aguiar Jde M,Bon-Frauches AC,Gomes AL,Veríssimo CP,Aguiar DP,Matias D,Thomasi BB,Gomes AS,Brito GA,Moura-Neto V

    更新日期:2015-06-01 00:00:00

  • Increased glutamate uptake and glutamine synthetase activity in neuronal cell cultures surviving chronic hypoxia.

    abstract::To examine the neurochemical effects of chronic hypoxia on immature nervous tissue in vitro, mixed neuronal-glial cell cultures derived from fetal mice were exposed to 5% O2 for 24 or 48 h. Those cultures subjected to longer hypoxia manifested improved neuronal survival compared to those with the shorter insult, both ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440030506

    authors: Sher PK,Hu SX

    更新日期:1990-01-01 00:00:00

  • Downregulation of Kir4.1 inward rectifying potassium channel subunits by RNAi impairs potassium transfer and glutamate uptake by cultured cortical astrocytes.

    abstract::Glial cell-mediated potassium and glutamate homeostases play important roles in the regulation of neuronal excitability. Diminished potassium and glutamate buffering capabilities of astrocytes result in hyperexcitability of neurons and abnormal synaptic transmission. The role of the different K+ channels in maintainin...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20455

    authors: Kucheryavykh YV,Kucheryavykh LY,Nichols CG,Maldonado HM,Baksi K,Reichenbach A,Skatchkov SN,Eaton MJ

    更新日期:2007-02-01 00:00:00

  • Blood monocytes and spleen macrophages differentiate into microglia-like cells on monolayers of astrocytes: membrane currents.

    abstract::Microglia, the resident macrophages of the central nervous system (CNS), can be distinguished from most other cells of the myelomonocytic lineage by a distinct pattern of membrane currents. In the accompanying paper we have shown that the characteristic morphological feature of microglia, ramification, develops both i...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440120403

    authors: Schmidtmayer J,Jacobsen C,Miksch G,Sievers J

    更新日期:1994-12-01 00:00:00

  • Type I interferon pathway in CNS homeostasis and neurological disorders.

    abstract::Type I interferons (IFNs), IFN-α and IFN-β, represent the major effector cytokines of the host immune response against viruses and other intracellular pathogens. These cytokines are produced via activation of numerous pattern recognition receptors, including the Toll-like receptor signaling network, retinoic acid-indu...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.23154

    authors: Blank T,Prinz M

    更新日期:2017-09-01 00:00:00

  • Astrocytes, the rising stars of the glioblastoma microenvironment.

    abstract::Glioblastoma (GBM) is an aggressive primary tumor, causing thousands of deaths worldwide every year. The mean survival of patients with GBM remains below 20 months despite current available therapies. GBM cells' interactions with their stromal counterparts are crucial for tumor development. Astrocytes are glial cells ...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.23520

    authors: Brandao M,Simon T,Critchley G,Giamas G

    更新日期:2019-05-01 00:00:00

  • Analysis of ion channel expression by astrocytes in red nucleus brain stem slices of the rat.

    abstract::The red nucleus (RN) has been widely used to study the formation and remodeling of synaptic connections during development and in post-lesion plasticity. Since glial cells are thought to contribute to synaptic plasticity, and information on functional properties of brain stem glia is missing, we analyzed voltage-gated...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Akopian G,Kuprijanova E,Kressin K,Steinhüser C

    更新日期:1997-03-01 00:00:00

  • Age-related changes in astrocytic and ependymal cells of the subventricular zone.

    abstract::Neurogenesis persists in the adult subventricular zone (SVZ) of the mammalian brain. During aging, the SVZ neurogenic capacity undergoes a progressive decline, which is attributed to a decrease in the population of neural stem cells (NSCs). However, the behavior of the NSCs that remain in the aged brain is not fully u...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22642

    authors: Capilla-Gonzalez V,Cebrian-Silla A,Guerrero-Cazares H,Garcia-Verdugo JM,Quiñones-Hinojosa A

    更新日期:2014-05-01 00:00:00

  • Patterned distribution of immunoreactive astroglial processes in the striate (V1) cortex of New World monkeys.

    abstract::The possibility of a modular organization of non-neuronal elements was analyzed in the opercular region of the striate neocortex in adult New World monkeys. For this purpose, and in order to follow possible correlations in the general organization of neuronal and astroglial elements, immunocytochemical procedures for ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Colombo JA,Schleicher A,Zilles K

    更新日期:1999-01-01 00:00:00

  • Transport of L-arginine in cultured glial cells.

    abstract::Uptake of radiolabelled L-arginine was studied in four different kinds of glial cultures, in astroglia-rich primary cultures derived from neonatal rat and mouse brains, in pure murine astrocyte cultures, and in rat glioma cells C6-BU-1. A saturable component of uptake was found in all cases with KM values between 15 a...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440110307

    authors: Schmidlin A,Wiesinger H

    更新日期:1994-07-01 00:00:00

  • Puma, but not noxa is essential for oligodendroglial cell death.

    abstract::The mechanisms involved in oligodendroglial cell death in human demyelinating diseases are only partly understood. Here, we demonstrate that the BH3 only protein Puma, but not Noxa, is essential for oligodendroglial cell death in toxic demyelination induced by the copper chelator cuprizone. Primary oligodendrocytes de...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22552

    authors: Hagemeier K,Lürbke A,Hucke S,Albrecht S,Preisner A,Klassen E,Hoffmann E,Cui QL,Antel J J,Brück W,Klotz L,Kuhlmann T

    更新日期:2013-10-01 00:00:00

  • Atrial natriuretic peptide-like (ANP-LIR) and ANP prohormone immunoreactive astrocytes and neurons of human cerebral cortex.

    abstract::Atrial natriuretic peptide (ANP) represents a family of related peptides originally isolated from cardiac atria that have potent natriuretic, diuretic, and vasorelaxant properties. ANP has previously been localized in neurons of the rat brain in regions subserving cardiovascular functions and fluid/electrolyte balance...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440120308

    authors: McKenzie JC,Berman NE,Thomas CR,Young JK,Compton LY,Cothran LN,Liu WL,Klein RM

    更新日期:1994-11-01 00:00:00

  • Cathepsin S release from primary cultured microglia is regulated by the P2X7 receptor.

    abstract::Microglia respond rapidly to injury, increasing their synthesis and release of inflammatory mediators, many of which contribute to the maintenance of persistent pain following CNS or PNS injury. We have recently shown that the lysosomal cysteine protease Cathepsin S (CatS) expressed by spinal microglia is vital for th...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.21042

    authors: Clark AK,Wodarski R,Guida F,Sasso O,Malcangio M

    更新日期:2010-11-01 00:00:00

  • Astrocytic GABA receptors.

    abstract::GABA receptors are distributed widely throughout the central nervous system on a variety of cell types. It has become increasingly clear that astrocytes, both in cell culture and tissue slices, express abundant GABAA receptors. In astrocytes, GABA activates Cl(-)-specific channels that are modulated by barbiturates an...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.440110203

    authors: Fraser DD,Mudrick-Donnon LA,MacVicar BA

    更新日期:1994-06-01 00:00:00

  • Beta IV tubulin is selectively expressed by oligodendrocytes in the central nervous system.

    abstract::Oligodendrocyte differentiation and myelination involve dramatic changes in cell signaling pathways, gene expression patterns, cell shape, and cytoskeletal organization. In a pilot study investigating CNS angiogenesis, oligodendrocytes were intensely labeled by antisera directed against the C-terminal of Tie-2, a 140-...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20175

    authors: Terada N,Kidd GJ,Kinter M,Bjartmar C,Moran-Jones K,Trapp BD

    更新日期:2005-05-01 00:00:00

  • Microglia, a potential source of neurons, astrocytes, and oligodendrocytes.

    abstract::Microglia are considered the only cell population of mesodermal origin in the brain, although their role is not fully understood. The present study demonstrated that rat primary microglial cells expressed nestin, A2B5, and O4 antigens, which are markers for oligodendrocyte precursor cells. Based on these findings, we ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.10306

    authors: Yokoyama A,Yang L,Itoh S,Mori K,Tanaka J

    更新日期:2004-01-01 00:00:00

  • Activated glia induce neuron death via MAP kinase signaling pathways involving JNK and p38.

    abstract::Chronic glial activation in neurodegenerative diseases contributes to neuronal dysfunction and neuron loss through production of neuroinflammatory molecules. However, the molecular mechanisms, particularly the signal transduction pathways involved in glia-dependent neuron death, are poorly understood. As a first step ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.10314

    authors: Xie Z,Smith CJ,Van Eldik LJ

    更新日期:2004-01-15 00:00:00

  • Insulin-like growth factor I: a mitogen for rat Schwann cells in the presence of elevated levels of cyclic AMP.

    abstract::To develop effective procedures for improving the regeneration of peripheral nerves and for preventing the formation of neurofibromas, it is necessary to identify the different mitogens that stimulate the proliferation of Schwann cells. Insulin-like growth factor I (IGF-I), which is a potent autocrine growth factor in...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440080403

    authors: Schumacher M,Jung-Testas I,Robel P,Baulieu EE

    更新日期:1993-08-01 00:00:00