BmNPV-miR-415 up-regulates the expression of TOR2 via Bmo-miR-5738.

Abstract:

:MicroRNAs (miRNAs) have emerged as key players in host-pathogen interaction and many virus-encoded miRNAs have been identified (computationally and/or experimentally) in a variety of organisms. A novel Bombyx mori nucleopolyhedrosis virus (BmNPV)-encoded miRNA miR-415 was previously identified through high-throughput sequencing. In this study, a BmNPV-miR-415 expression vector was constructed and transfected into BmN cells. The differentially expressed protein target of rapamycin isoform 2 (TOR2) was observed through two-dimensional gel electrophoresis and mass spectrometry. Results showed that TOR2 is not directly a target gene of BmNPV-miR-415, but its expression is up-regulated by BmNPV-miR-415 via Bmo-miR-5738, which could be induced by BmNPV.

journal_name

Saudi J Biol Sci

authors

Cao X,Huang Y,Xia D,Qiu Z,Shen X,Guo X,Zhao Q

doi

10.1016/j.sjbs.2015.09.020

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

1614-1619

issue

7

eissn

1319-562X

issn

2213-7106

pii

S1319-562X(15)00215-6

journal_volume

24

pub_type

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